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The Anticancer Properties of Lunasin Peptide from Aged Callus Induced by the Soybean Tissue Culture  

Park, Jae-Ho (School of Bioresources, Andong National University)
Jeong, Jin-Boo (School of Bioresources, Andong National University)
De Lumen, Ben O. (Division of Nutritional Science & Toxicology, University of California)
Jeong, Hyung-Jin (School of Bioresources, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.20, no.6, 2007 , pp. 518-523 More about this Journal
Abstract
Lunasin is small subunit peptide of coded from Gm2S-1 gene in soybean. It has been previously demonstrated that lunasin is a novel and promising cancer preventive peptide. Lunasin peptide is found only in the seed and not other tissues. And lunasin peptide starts to appear at 5 weeks after flowering and remains in the mature seed. We report here firstly lunasin peptide identified from soybean callus induced by the tissue culture and demonstrate its anticancer properties. The lunasin was identified and purified from soybean callus aged for 6 months. The callus lunasin($1{\mu}M$) inhibited the acetylation of histone H3 and H4 by 58.8% and 56.5%, respectively. And it fully inhibited foci formation compared to the values of the positive control(no lunasin) and negative control(no MCA). Purified lunasin was able to internalize into the cell and localized in the nucleus.
Keywords
Lunasin; Gm2S-1; Embryo culture; Histone acetylation;
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1 De Lumen, B.O., A.F. Galvez, J.M. Revilleza and D.C. Krenz. 1999. In Chemicals via Higher Plant Bioengineering, Advances in Experimental Medicine and Biology; Shahidi, et al., Eds.; Kluwer Academic: New York 464: 117-126
2 De Lumen, B.O. 2005. Lunasin: A Cancer-preventive soy peptide, Nutr. Rev. 63: 16-21   DOI
3 Galvez, A.F. and De Lumen B.O. 1997. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat. Biotechnol. 17: 495-500
4 Lam, Y., A.F. Galvez and B.O. De Lumen. 2003. Lunasin suppresses EIA-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr. Cancer 47(1): 88-94   DOI   ScienceOn
5 Tanner, K.G., M.R. Langer, Y. Kim and J.M. Denu. 2000. Kinetic mechanism of the histone acetyltransferase GCN5 from yeast. J. BioI. Chem. 29(275): 22048-22055
6 Yasui W., N. Oue, S. Ono, Y. Mitani, R. Ito and H. Nakayama. 2003. Histone acetylation and gastrointestinal carcinogenesis. Ann. N.Y. Acad. Sci. 983: 220-231   DOI
7 Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254   DOI   ScienceOn
8 Jeong, H.J., J.B. Jeong, D.S. Kim and B.O. De Lumen. 2007. Inhibition of Core Histone Acetylation by the Cancer Preventive Peptide Lunasin. J. Agric. Food Chem. 55: 632-637   DOI   ScienceOn
9 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497   DOI
10 Galvez, A.F., M. Jamela, R. Revilleza and B.O. De Lumen. 1997. A novel methionine-rich protein from soybean cotyledon: cloning and characterization of eDNA. Plant Physiol. 114: 1567-1569   DOI
11 Kim, Y., K.G. Tanner and L.M. Denu. 2000. A continuous, nonradioactive assay for histone acetyltransferases. Anal. Biochem. 280: 308-314   DOI   ScienceOn
12 Jeong, H.J., J.H. Park, Y. Lam and B.O. De Lumen. 2003. Characterization of lunasin isolated from soybean. J. Agric. Food Chem. 51: 7901-7906   DOI
13 Luo, R.X. and D.C. Dean. 1999. Chromatin Remodeling and Transcriptional Regulation. J. Natl. Cancer lnst. 91: 1288-1294   DOI
14 Shogren-Knaak, M., H. Ishii, J.M. Sun, M.J. Pazin, J.R. Davie and C.L. Peterson. 2006. Histone H4-K16 acetylation controls chromatin structure and protein interactions, Science 311: 844-847   DOI   ScienceOn
15 Galvez, A.F., N. Chen, J. Macasieb and B.O. De Lumen. 2001. Chemopreventive property of a soybean peptide that binds to deacetylated histones and inhibits histone acetylation. Cancer Res. 61: 7473-7478
16 Hunter, G.A. and G.C. Ferreira. 1995. Continuous spectrophotometric assay for 5-aminolevulinate synthase that utilizes substrate cycling. Anal. Biochem. 226: 221-224   DOI   ScienceOn