• Advances in pediatric surgery
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• v.9 no.2
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• pp.94-97
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• 2003

The teratoma is a unique complex neoplasm and is one of the most frequent pediatric tumors originated from the extragonadal germ cells. Mature teratoma is composed of mature differentiated tissues, while immature teratoma always contains embryonic tissues of variable degrees of immaturity, especially in the neuroepithelial elements. Diagnosis of teratoma is relatively easy by conventional radiologic study, but the immaturity can be identified only by histopathological examination. Between January 1993 to December 2002, 63 cases of teratoma were operated and analysed retrospectively at the Chonnam University Hospital Female to male ratio was about 3:1 and age distribution was relatively even. Among 63 cases, gonadal teratoma was the most common (52.4%), followed by sacrococcygeal (25.4%), retroperitoneal (9.5%) and mediastinal teratoma (9.5%). Fifty-six cases were mature teratomas and seven were immature teratomas. Alpha-fetoprotein (AFP) was elevated in 4 of 6 immature cases, but in 2 of 51 mature ones Elevated AFP progressively returned to normal range by 1 month after operation in all. Complete excision of the mass was performed, and major complication was not noticed. In five immature cases, PEB chemotherapy (Cisplatin, Etoposide, Bleomycin) was performed. Two of 2 cases in histological grade II were well tolerated to the aggressive chemotherapy. One of three cases in grade III expired due to severe bone marrow depression, and two of them expired by tumor recurrences. In conclusion, immature teratoma in histological grade III showed high potentiality of recurrence. Therefore, postoperative chemotherapy has to be applied to the high graded immature tumors.

• Journal of the Korean Society of Food Science and Nutrition
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• v.11 no.1
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• pp.15-20
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• 1982

The fatty acid composition of human milk was determined in 20 milk samples (ten colostrum and 10 mature) obtained at different stages of lactation. Human colostrum contained a lower percentage of total lipids than mature milk. In comparison with mature milk, human colostrum was characterized by a lower percentage of saturated fatty acids, a higher percentage of monounsaturated fatty acids and a higher percentage of extra-long-chain polyunsaturated fatty acid metabolites of both linoleic acid ($\omega$ 6 series) and linolenic acid ($\omega$ 3 series). The linoleic acid levels reported here are considerably higher than those reported previously in Korea. This shift has paralleled an increase in the use of vegetable oils in Korean diet. The human mature milk differed from marketing cow's milk in fatty acid composition, as it had a lower content of short-, medium-and long-chain saturated fatty acids and a higher content of long-chain monounsaturated and polyunsaturated fatty acids.

• Food Science and Preservation
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• v.9 no.2
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• pp.148-153
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• 2002

Effect of the thickness of low density polyethylene(LDPE) Film as packaging material on changes in physicochemical characteristics of mature-green mume (Prunus mume Sieb. et Zucc) fruits during storage at different temperatures(0, 10 and 25$\^{C}$) were investigated. Fruit samples stored in pouches with 30㎛ thickness of LDPE film maintained well not only external qualities such as Shape and firmness, but also internal chemical properties such as pH, acidity, the content of soluble solid and chlorophyll, and color, comparing with samples stored without packaging material or in other pouches with different film thickness(20 and 40㎛). From the results, it was found that the maintenance of quality of mature-green mume fruits during storage at 0, 10 and 25$\^{C}$ closely related to the changes in respiratory conditions for fruits affected by the film thickness of the packaging material.

• Animal cells and systems
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• v.16 no.2
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• pp.162-171
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• 2012

We investigated food webs of a $Salix$ $subfragilis$-dominated wetland in the Janghang wetland in the Hangang estuary, which is very close to the Demilitarized Zone, along the west coast of Korea. Our study focused on understanding sesarmine crab ($Sesarma$ $dehaani$)-related food webs in a $S.$ $subfragilis$ forest. For our study, we used carbon and nitrogen stable isotopes and fatty acid biomarkers. We collected samples of plants, animals, and detrital sediment from four quadrats ($5{\times}5m^2$) set in the $S.$ $subfragilis$ community. Samples were collected from September 2006 to June 2009, except during the winter hibernation period of $S.$ $dehaani$. In the wet season, the sediment showed relatively high ${\delta}^{13}C$ and low ${\delta}^{15}N$ signatures compared with relatively low ${\delta}13C$ and high ${\delta}15N$ signatures in the dry season. Mature $S.$ $dehaani$ appeared to feed on fresh leaves and other carbon sources, such as immature individuals or fish, in addition to detrital sediment, which appeared to be the main carbon source for immature crabs. Principal component analysis of fatty acid biomarkers of $S.$ $dehaani$ showed a clear difference between immature individuals (10-30 mm) and mature ones (larger than 30 mm), indicating that the main food source for immature crabs was detrital sediment, whereas mature crabs foraged plants in addition to consuming detrital sediment. On the basis of our results from stable isotope and fatty acid analyses, mature $S.$ $dehaani$ appeared to feed on detrital sediment and fresh leaves of $S.$ $subfragilis$ in summer in addition to engaging in cannibalism of immature individuals.

• Korean Journal of Microbiology
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• v.30 no.5
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• pp.403-409
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• 1992

For the development of a glucanolytic yeast strain. the seceretion of endo-1.4-$\beta$-D-glucanase (CMCase) of Bacillus subtilis was performed in yeast using glucoamylase gene (STA1) of Saccharomyces diastaticus. A 1.7 kb-DNA fragment of STA1 gene containing authentic promoter, signal sequence, threonine serine-rich (TS) region and N-terminal region (98 amino acids) of mature glucoamylase was ligated to YEp 24. E. coli-yeast shuttle vector. And then. CMCase structural gene of B. subtilis was fused in frame with the 1.7 kb-DNA fragment of STA1 gene, resulting in recombinant plasmid pYES('24. Yeast transformant harboring pYESC24 had no CMCase activity. So. we deleted TS region and N-terminal region of mature glucoamylase existing between signal sequence and CMCase structural gene in pYESC24. consequently constructed recombinant plasmid pYESC11. The yeast transformed with the newly constructed recombinant plasmid pYESC11 efficiently secreted CMCase to extracellular medium. After 4 days culture. total CMCase activity of this transformant was 44.7 units/ml and over 93% of total CMCase activity was detected in culture supernatant.

• The Korean Journal of Ecology
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• v.18 no.1
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• pp.189-201
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• 1995

In order to clarify regeneration processes and mechanisms of the disturbed Pinus densiflora forest, responses of Pinus densiflora to gap formed by disturbance were analysed by growth of saplings and mature and growth equations were obtained from branch growth of mature trees and height growth of saplings, and age distribution of saplings and young trees recruited within gap was analysed in relation to gap age. Height growth of saplings within gaps was accelerated after gap formation. Such abrupt increases of growth of saplings after the gap formation might be resulted in the difference of growth of saplings between gap and non-gap areas. In fact, height and diameter of saplings in the central part of gap were larger than those of saplings in marginal parts of gap and non-gap area. However, density of saplings was not different in both parts. In addition, growth of annual rings of mature trees bordering on gap also increased after gap formation. Branch growth of mature trees bodering on gap was 6.3 - 6.5 cm /year and the mean radius of gaps created by death of only one canopy tree was about 3 m. Therefore, for those gaps to be closed by branch growth it will take 46 years. Growth of saplings within gap showed exponential equation. Fifty years will be required for the saplings to enter the forest canopy by the exponential growth equation. Therefore, gap created by only one tree might be closed by branch growth of surrounding canopy trees in advance of being done by height growth of saplings. But gaps created by death of trees more than 2 will be closed by the growth of saplings. Among the regenerating saplings and young trees within gaps, individuals established in advance of gap formation were more than those established after the gap formation. From these results, it was assumed that the disturbed Pinus densiflora forests in these sites were regenerated by height growth of saplings recruited in advance of gap formation.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.55-64
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• 2012

Ultrastructural studies of germ cell development during spermatogenesis and taxonomic values in mature sperm morphology of Argopecten irradians irradians were investigated by transmission electron microscopic observations. In the early stage of spermatid during spermiogenesis, a few granules and proacrosomal granules are formed by the Golgi complex. In the late stage of spermatid during spermiogenesis, a proacrosomal vesicle becomes an acrosomal vesicle in the acrosome through spermiogenesis. The sperm is approximately $ 45-48{\mu}m$ in length including a jar-shaped sperm nucleus (about $1.45{\mu}m$ long), an acrosome (about $0.34{\mu}m$ long) and tail flagellum. The axoneme of the sperm tail shows a 9+2 structure. As one of common characteristics of mature sperm morphologies in Pectinidae species in subclass Pteriomorphia, mature spermatozoon consists of the cone-shaped acrosomal vesicle and subacrosomal material on the invaginated jar-shaped nucleus. The acrosomal vesicle of this species is composed of electron high dense opaque part (material) from the base to the tip, as have seen in the species in the subclass Pteriomorphia. Exceptionally, five mitochondria are found in the sperm midpiece of this species, unlike four in most species of Pectinidae in subclass Pteriomorphia. However, the acrosomal vesicle of spermatozoa of A. irradians irradians resemble to those of other investigated Pectinidae species in subclass Pteriomorphia. Therefore, we can use sperm morphology as a tool in the resolution of taxonomic relationships within the Pectinidae species. These morphological charateristics of acrosomal vesicle belong to the family Pectinidae in the subclass Pteriomorphia.

• The Korean Journal of Ecology
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• v.25 no.4
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• pp.261-265
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• 2002

In order to elucidate the characteristics of standing crop biomass, production and nutrient content of dead bole in mature ecosystem, we surveyed the dynamics of decaying bole of old-aged deciduous forest in 1993 and 2002 in Kwangnung Experimetal Forest Station. In addition, we and estimated annual bole production, water content, wood density and nutrient content and compared the results with that of temperate ecosystem. Total dead wood biomass was estimated to be 5.6ton/ha in 1993 and 17.6 ton/ha in 2002. Standing dead tree accounted for a total of 1.1 ton/ha in 1993 and 4.8 ton/ha in 2002, which was 20% and 27$\%$ of the sum of dead bole mass in 1993 and 2002, respectively. Annual production of bole biomass was 1.3 ton/ha/yr. These values fall into the low range of dead wood biomass for the mature temperate ecosystems. Tree species composing standing bole was mainly Quercus and Carpinus trees. This bole species composition resembles alive species composition of this forest. Water content of bole increased as positive logarithmically, but wood density of bole decreased as negative exponentially along with the progress of decay. N, P, Ca and Mg concentrations in decaying boles generally increased with decay, except for K. Annual nutrient input via dead bole is 1.6 kg/ha/yr for N, 0.04 kg/ha/yr for P, 1.0 kg/ha/yr for K, 1.7 kg/ha/yr for Ca and 0.3 kg/ha/yr for Mg, respectively.

• Journal of the Korean Wood Science and Technology
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• v.21 no.2
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• pp.73-80
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• 1993

Pinus koraiensis is one of the most important reforestation species which had widely been planted around the country. Therefore, there are great research needs on the fundamental properties of wood to extend the frequency of uses and adequate availabilities. Research results on the characteristics of anatomical wood properties and changes are summarized as follows: 1. At a horizontal direction of tree trunk, the tracheid length at outer wood gradually becomes shorter as it reaches to pith. The tracheid length having between 32 and 42 annual rings showed over 2.5 times longer than that of pith, and also its length increases from the bottom to the top of the stem. 2. The tracheid diameters in latewood showed a little fluctuations, and the shortest near to the pith in earlywood. The tracheid diameters in earlywood are more than 2 times those of latewood, and the differences between the mature and juvenile wood in earlywood are less than 10${\mu}m$. 3. The tracheid wall thickness in early wood showed a little fluctuations. On the contrary, tracheid wall thickness in latewood rapidly increases from the pith to the bark. The tracheid wall of mature wood showed 10 to 20% thicker than that of juvenile wood. 4. The fibril angle at the secondary walls of tracheid within an annual ring showed higher degree in earlywood than that of latewood. The highest tracheid fibril angle was observed at around the pith of both earlywood and latewood. Then, fibril angle slowly decreased toward outer wood, and was stabilized after 15 years. 5. Structural boundaries between mature and juvenile wood from Pinus koraiensis are divided by 13 to 19 annual ring and distance of 5 to 8cm from the pith.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.123-128
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• 2017

Hangover due to alcohol consumption causes social and physical problems. There is a growing interest in edible insects worldwide. We have previously published a new technology to make hard mature silkworm, Bombyx mori, into edible form, steamed and freeze-dried mature silkworm larval powder (SMSP). In this study, AIN-76 or SMSP (0.1 and 1 g/kg rat body weight) containing diets in SD rats were pretreated for 2 weeks, and ethanol (3 g/kg rat body weight) was administered as an oral gavage and sacrificed after 3 hours. As a result, blood alcohol and aldehyde levels were significantly decreased in SMSP fed rats. In addition, liver injury markers, alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly decreased in SMSP group compared to ethanol group. $TNF-{\alpha}$, an inflammatory cytokine, and malondialdehyde (MDA), an oxidative stress marker, also showed a dose-dependent decrease in the group receiving SMSP. Conclusively, consumption of SMSP not only reduced hangover induced by ethanol, but also decreased liver damage, oxidative stress, and inflammatory response.