• Geophysics and Geophysical Exploration
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• v.11 no.1
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• pp.68-77
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• 2008

We have developed an inversion algorithm for loop-loop electromagnetic (EM) data, based on the localised non-linear or extended Born approximation to the solution of the 2.5D integral equation describing an EM scattering problem. Source and receiver configuration may be horizontal co-planar (HCP) or vertical co-planar (VCP). Both multi-frequency and multi-separation data can be incorporated. Our inversion code runs on a PC platform without heavy computational load. For the sake of stable and high-resolution performance of the inversion, we implemented an algorithm determining an optimum spatially varying Lagrangian multiplier as a function of sensitivity distribution, through parameter resolution matrix and Backus-Gilbert spread function analysis. Considering that the different source-receiver orientation characteristics cause inconsistent sensitivities to the resistivity structure in simultaneous inversion of HCP and VCP data, which affects the stability and resolution of the inversion result, we adapted a weighting scheme based on the variances of misfits between the measured and calculated datasets. The accuracy of the modelling code that we have developed has been proven over the frequency, conductivity, and geometric ranges typically used in a loop-loop EM system through comparison with 2.5D finite-element modelling results. We first applied the inversion to synthetic data, from a model with resistive as well as conductive inhomogeneities embedded in a homogeneous half-space, to validate its performance. Applying the inversion to field data and comparing the result with that of dc resistivity data, we conclude that the newly developed algorithm provides a reasonable image of the subsurface.

• Journal of Korean Society of Transportation
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• v.22 no.7 s.78
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• pp.99-106
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• 2004

This paper presents some comparisons between Cournot-Nash and Stackelberg game in bi-level program, composed of both upper level program and lower level one. The upper level can be formulated to optimize a specific objective function, while the lower formulated to express travelers' behavior patterns corresponding to the design parameter of upper level problem. This kind of hi-level program is to determine a design parameter, which leads the road network to an optimal state. Bi-level program includes traffic signal control, traffic information provision, congestion charge and new transportation mode introduction as well as road expansion. From the view point of game theory, many existing algorithms for bi-level program such as IOA (Iterative Optimization Assignment) or IEA (Iterative Estimation Assignment) belong to Cournot-Nash game. But sensitivity-based algorithms belongs to Stackelberg one because they consider the reaction of the lower level program. These two game models would be compared by using an example network and show some results that there is no superiority between the models in deterministic case, but in stochastic case Stackelberg approach is better than that of Cournot-Nash one as we expect.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.9
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• pp.1239-1246
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• 2016

The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.833-841
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• 2013

In the case of composite material, a variety of characteristics were expressed depending on the materials that were composed of. In this study, the materials showing non-linear shear behavior were investigated among FRP composite. Each specimen was designed and analyzed according to ASTM D4255 method: regulations on the 2-rail. The dependent variables included in this experiment were a variety of fiber, fiber volume ratio, fiber array direction, temperature, material homogeneity. For determination of characteristics based on the fiber array, fiber array direction of 0, 30, 45, and 60 degrees were selected for test specimen. Temperature of 25, 40, 60, and $80^{\circ}C$ were considered for investigation of FRP materials'shear behavior based on the external temperature. Nonlinear shear behavior was observed throughout the FRP composite material in this study. Also, using vinyl ester resins, high fiber volume ratio, and fiber array direction of 45 degree appeared to show the most prominent nonlinear shear behavior. As for the findings related to the temperature change, non-linear behavior was decreased as the external temperature increased. For factory manufactured product, non-linear behavior was relatively at parity in comparison to the behavior found in the hand lay-up FRP composite specimen.

• KSBB Journal
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• v.21 no.4
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• pp.279-285
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• 2006

We investigated the effects of immobilization chemistry on the yield of immobilization and the bioactivity of the immobilized enzymes. Trypsin as a model protein and macroporous polymer beads(Toyopearl AF 650M, Tosho Co., Japan) was used as a model matrix. Four methods were used to immobilize trypsin; covalent conjugation by reductive amination(at pH 10.0 and pH 4.0) and affinity interaction via streptavidin-biotin, and double-affinity interaction via biotin-streptavidin-biotin system. The covalent conjugation immobilized $3{\sim}4$ mg/ml-gel, ca. 3-fold higher than the affinity method. However, the specific activity of the covalently(pH 10.0) and affinity-immobilized trypsin(via streptavidin-biotin) are ca. 37% and 50%, respectively, of that of the soluble enzyme(on the low-molecular-weight BAPNA substrate). When the molecular size of a substrate increased, the affinity-immobilized trypsin showed higher clavage activity on insulin and BSA. This result seemed to indicate the streptavidin-biotin system allowed more steric flexibility of the immobilized trypsin in its interaction with a substrate molecule. To confirm this, we studied the molecular flexibility of immobilized trypsin using quartz crystal microbalance-dissipation. Self-assembled monolayers were formed on the Q-sensor surface by aminoalkanethiols, and gultaraldehyde was attached to the SAMs. Trypsin was immobilized in two ways: reductive amination(at pH 10.0) and the streptavidin-biotin system. The dissipation shift of the affinity-immobilized trypsin was $0.8{\times}10^{-6}$, whereas that of the covalently attached enzyme was almost zero. This result confirmed that the streptavidin-biotin system allowed higher molecular flexibility. These results suggested that the bioactivity of the immobilized enzyme be strongly dependent on its molecular flexibility.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.3
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• pp.16-20
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• 2018

Steel Fiber reinforced self-compacting concrete (SFRSCC) has been widely used in a number of structures, such as ordinary civil infrastructures, sky scrapers, nuclear power plants, hospitals, dams, channels and etc. Thanks to its short and discrete reinforcing fibers, its performance, including tensile strength, ductility, toughness and flexural strength gets much better in comparison with ordinary self-compacting concrete (SCC) without any reinforcing fibers. Despite all these aforementioned advantages of SFRSCC, its performance highly depends on fiber's orientation. In case of short discrete fibers, the orientation of fibers is completely random and cannot be controlled during pumping process. If fibers distribution inside hardened state concrete are randomly distributed, it leads to less resistance potential of concrete element, especially in terms of flexural and tensile strength. The maximum expected strength may not be achieved. Therefore, fiber alignment has been considered as one of the important factors in SFRSCC. To address this issue, this study investigates the effects of concrete matrix's density and inlet velocity on fiber alignment during the pumping process using a finite element method.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.360-365
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• 2007

The studies for the derivatization of 3-monochloropropane-1,2-diol (3-MCPD) were performed mainly as acylation with HFBI (heptafluorobutyrylimidazole), alkylation with PBA (phenylboric acid) and silylation with BSTFA (N,O-bis[trimethylsilyl]trifluoroacetamide). Also silylation with MTBSTFA(N-methyl-N-[tert.-butyldimethylsilyl] trifluoroacetamide) and acylation with MBTFA (N-Methyl-bis[trifluoro-acetamide]) were also considered. Except the TBDMS derivative of 3-MCPD, all the derivatives were detected well. The derivatives of 3-MCPD with HFBI, PBA and BSTFA showed below 10 ${\mu}g/kg$ which was sensitive enough to satisfy Korea maximum residue limit 0.3 mg/kg. Among the tested adsorbents, Extrelut20 and Florisil were evaluated as the proper adsorbents to eliminate the soy sauce matrix for 3-MCPD. Ethyl acetate was the most efficient eluent with good recovery rate. The desired surrogate compound and internal standard were 1,2-butanediol and 1,2-dibromo-3-chloropropane, respectively. The limit of detection for PB-MCPD and TMS-MCPD were 10.16 and 7.06 ${\mu}g/kg$ on GC/MSD, respectively. HFB-MCPD derivative showed the lowest detection limits 2.98 and 5.32 ${\mu}g/kg$ by GC/ECD and GC/MSD, respectively.

• Journal of Life Science
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• v.24 no.4
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• pp.437-446
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• 2014

Problems appear when triptans are taken orally. For example, the bioavailability of triptan is reduced by the digestive system, and the drug level in the blood reduces rapidly over time; there is also a possibility of gastrointestinal disorder. To improve side effects, a transdermal patch has been prepared in hydrogel form. The polymer matrix that makes up the hydrogel uses PVA; PEG is used as an additive to induce inter/intra hydrogen bonding of the PVA and almotriptan drug is added. In addition, to accelerate micro-phase separation between PVA chains, liquid nitrogen is used. In FT-IR analysis, the absorption bands of PVA, PEG, and almotriptan were found. The degree of crystallinity, the water uptake ability and tensile strength were increased with increasing PEG content. In drug release tests, the amount of drug released increased depending on the PEG content. In this study, hydrogels with 10 wt% PEG showed better performance in drug release. Approximately 60% of the total drug amount was released in 2 hr, and the drug continued to release for 1 day. Thus, the prepared hydrogel patch is suitable as a transdermal formulation for the second dose administration of triptans to patients who require recurrent migraine treatment within 24 hr after the first administration.

• Journal of the Korean Regional Science Association
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• v.33 no.1
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• pp.29-41
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• 2017

This study proposes an integrated accessibility measurement algorithm, which is applied to the Seoul Metropolitan public transportation system consisting of bus and subway networks, and analyzes the result. We construct a public transportation network graph linking bus-subway networks and take the time distance as the link weight in the graph. We develop a time-distance algorithm to measure the time distance between each pair of transit stations based on the T-card transaction database. The average travel time between nodes has been computed via the shortest-path algorithm applied to the time-distance matrix, which is obtained from the average speed of each transit route in the T-card transaction database. Here the walking time between nodes is also taken into account if walking is involved. The integrated time-distance accessibility of each node in the Seoul Metropolitan public transportation system has been computed from the T-card data of 2013. We make a comparison between the results and those of the bus system and of the subway system, and analyze the spatial patterns. This study is the first attempt to measure the integrated time-distance accessibility for the Seoul Metropolitan public transportation system consisting of 16,277 nodes with 600 bus routes and 16 subway lines.

• Analytical Science and Technology
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• v.29 no.6
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• pp.261-268
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• 2016

TXRF is a powerful technique for the soil sample analysis due to its ability to conduct quantitative analysis of powder sample without complicated pre-treatment processes. The conventional internal standard method used for this technique suffers from relatively low accuracy because of varying matrix effects of soil. In order to improve the accuracy, external standard method was applied to analyze two types of soil samples; acid-dissolutionized soil solution and detergent-suspended soil powder. Individual ICP-AES/MS grade standards were mixed, diluted and measured to create standard curves, but applying these curves for analyzing the soil solution sample did not make any improvement in comparison with the internal standard method. On the other hand, standard curves were created with using standard soil powders for the analysis of soil powder samples, and we found that this method increased the accuracy significantly relative to the internal standard method. Especially, Al, Fe, K, Ca, Ti, Ba, Mn, Sr, Rb, Cu was measured with relatively high accuracy (relative error = ${\pm}20%$).