• Korean journal of applied entomology
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• v.49 no.4
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• pp.409-416
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• 2010

An entomopathogenic bacterium, Photorhabdus temperata ssp. temperata (Ptt), suppresses insect immune responses and facilitates its symbiotic nematode development in target insects. The immunosuppressive activity of Ptt enhances pathogenicity of various microbial pesticides including Bacillus thuringiensis (Bt). This study was performed to select a cheap and efficient bacterial culture medium for large scale culturing of the bacteria. Relatively cheap industrial bacterial culture media (MY and M2) were compared to two research media, Luria-Bertani (LB) and tryptic soy broth (TSB). In all tested media, a constant initial population of Ptt multiplied and reached a stationary phase at 48 h. However, more bacterial colony densities were detected in LB and TSB at the stationary phase compared to two industrial media. All bacterial culture broth gave significant synergism to Bt pathogenicity against third instars of the diamondback moth, Plutella xylostella. Production of bacterial metabolites extracted by either hexane or ethyl acetate did not show any significant difference in total mass among four culture media. Reverse phase HPLC separated the four bacterial metabolites, which were not much different in quantities among four bacterial culture broths. This study suggests that two industrial bacterial culture media can be used to economically culture Ptt in a large scale.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.469-477
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• 2012

Ostrinia larvae feed the pods and stem of red bean and seriously damage the bean production from farmers. In this study we investigated biological and developmental characteristics including field collection, host feeding preference, artificial rearing diet, morphological and molecular taxonomical identification, and pheromone analysis for an Ostrinia sp. in Korea. The male adults have massive tibia in the middle legs and 3-lobed uncus in the genitalia. The partial nucleotide sequences of mitochondrial cytochrome oxidase I (COI) and II (COII) were not corresponded to those DNA sequences from other Ostrinia species reported previously in Japan and China. Host plants for this species are also different from the previous species reported. In the gas chromatography (GC) analyses, (Z)-9-tetradecenyl acetate was not detected from the pheromone gland of our species while the component as a sex pheromone was found in O. zaguliaevi and O. zealis, With taken results, we conclude this Ostrinia species in Korea is Ostrinia scapulalis or closely related species. When larvae collected in a fall were incubated in the outdoor condition, they emerged to adult between June and July in the next year. The result indicates that the winter diapause could be started in late larval stage. In addition, we developed a semi-synthetic artificial diet adopted for mass rearing of the O. scapulalis in laboratory.

• Korean journal of communication and information
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• v.71
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• pp.194-223
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• 2015

This study explores a value chain structure of TV program including news, drama, and comedy. The purpose of this research focused on factor analysis and the relationship among viewer's program selection motivations. This research explores correlation between personal value and viewing motivation. This study was to identify the value structure of respondent on TV program(news, drama, comedy) based on means-end chain theory. The research used structured APT laddering questions and 251 data was analysed. Through such analysis, category difference by stage and relationship difference were identified and hierarchical value map was compared. There are four different value ladders: first is attributes, functional consequences, psychological consequences, and final value. The result shows that on news program the basic function is viewers are want to visual factor and quickly acquire social news and they pursue a value of personal social relationship. Whereas, on drama program, the viewers are reflected by around person, and they selected a program based on closed related person. In addition, the viewers are influenced by program's social nomination, production's brand in drama, and performer's nomination, producer and program prominence on comedy. The program selection is highly correlated on program selection's credibility, vital energetic life, and social relationship. The results shows that there was no significant difference between low involvement group and high involvement group for main category involvement group respondents.

• Journal of Science and Technology Studies
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• v.1 no.2 s.2
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• pp.343-369
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• 2001

Understanding of the social perception of biotechnology would facilitate the public awareness and debate over the social implications of biotechnology, leading to strengthened basis for social consensus. As a part of the ELSI (Ehical, Legal and Social Implications) project in Korea, the authors have launched a series of social surveys on the social perception of biotechnology. This article is based on the analysis of the first survey, conducted in October 2001. The data were collected through telephone survey on 500 adult respondents nationwide selected by a stratified sampling method. The survey addresses the following questions: What is the present state of public awareness and attitude toward a variety of medical and social applications of biotechnology, such as genetic testing, prenatal genetic screening and testing, xenotransplant, genetic screening for employment, central collection and management of genetic information, and GM food? What factors are related with this perception? The analysis shows that a majority of respondents are in favor of the medical applications. Concerning the social applications and GM food, however, the respondents express a high level of negative attitude a with significant portion of 'do not know' responses. The public perception of the biotechnology is not crystallized in coherent manner yet. The public perception is strongly influenced by mass media, which tend to deliver rather positive information on biotechnology. The analysis suggests that the production and dissemination of diverse information should be activated to reach a sound decision on controversial issues surrounding the development of biotechnology both at individual and societal level as well.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.46-52
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• 2003

This experiment was conducted to investigate the propagation for mass production of Acanthopanax koreanum NAKAI utilized for health food and medicine material. Germination rate of A. koreanum seeds were 64% when seeds were stored at 15 for 60 days and then were treated with 50 ppm of kinetin and dormancy were broken at 5 for 60 days. Rooting rates of green-wood cuttings treated with IBA 100 ppm, NAA 50 ppm, and IAA 100 ppm were 61.7, 56.7, and 60.0%, respectively. Rooting rates of greenwood cuttings treated with Rooton in scoria + horticulture media and volcanic ash were 76.7 and 66.7%, respectively. Survival rate of seedlings planted on Aug. 10 was highest (96%) under 75% shading net while shoot growth was best under 55% shading net. Survival rate of seedlings planted on May 2 was highest (91%) 55% shading net, and shoot growth was also best 55% under shading net.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.1
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• pp.73-80
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• 2004

In this paper, the implementation and embedding method of the existing air-filled waveguide-filters at millimeter-wave on general PCB substrate is introduced by systematically inserting the vias inside waveguide and mathematically manipulating the simple equations obtained ken the classical circular-post waveguide filter design. All the metal structures placed vertically such as side wall fur perfect ground plane and circular-post for signal control in the air-filled WR-22 waveguide are replaced with several types of via for constructing the bandpass-filter. Side wall and poles inside waveguide are realized by placing a series array of via and tuning the via diameter. The lengths of x, y, z axis are reduced in proportion to root square of employed substrate dielectric constant and especially the length of z axis can be more reduced due to the characteristics of the wave propagation. Because the mass production on PCB is possible without fabricating a large-scaled metal waveguide of WR-22 as input/output ports at millimeter-wave regime, the manufacturing cost is reduced considerably. Finally, when using multilayer process like LTCC for small-sized module, it is one of advantages to use only one layer f3r the filter fabrication. To evaluate the validity of this novel technique, order-3 Chebyshev BPF(Bandpass-Filter) centered at 40 GHz-band with a 2.5 ％ FBW (Fractional Bandwidth) were used. The employed substrate has relative dielectric constant of 2.2 and thickness of 10 mil of Rogers RT/Duroid 5880. Accroding to design and measurement results, a good performance of insertion loss of 2 ㏈ and return loss of -30 ㏈ is achieved at full input/output ports.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.584-592
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• 2004

A thermostable $\beta$-glycosidase gene, tfi $\beta$-gly, was cloned from the genomic library of Thermus filiformis Wai33 A1. ifi $\beta$-gly consists of 1,296 bp nucleotide sequence and encodes a polypeptide of 431 amino acids. It shares a strong amino acid sequence similarity with the $\beta$-glycosidases from other Thermus spp. belonging to the glycosyl hydrolase family 1. In the present study, the enzyme was overexpressed in Escherichia coli BL21 (DE3) using the pET21b(+) vector system. The recombinant enzyme was purified to homogeneity by heat treatment and a $Ni^{2+}$-affinity chromatography. Polyacrylamide gel electrophoresis (PAGE) showed that the recombinant Tfi $\beta$-glycosidase was a monomeric form with molecular mass of 49 kDa. The temperature and pH range for optimal activity of the purified enzyme were 80- $90^{\circ}C$ and 5.0-6.0, respectively. Ninety-three percent of the enzyme activity was remained at $70^{\circ}C$ after 12 h, and its half-life at $80^{\circ}C$ was 6 h, indicating that Tfi $\beta$-glycosidase is highly thermostable. Based on its K_m$, or $K_{cat}K_m$, ratio, Tfi $\beta$-glycosidase appeared to have higher affinity for $\beta$-D-glucoside than for $\beta$-D-galactoside, however, $K_{cat} for \beta$-D-galactoside was much higher than that for $\beta$-D-glucoside. The activity for lactose hydrolysis was proportionally increased at $70^{\circ}C$ and pH 7.0 without substrate inhibition until reaching 250 mM lactose concentration. The specific activity of Tfi TEX>$\beta$-glycosidase on 138 mM lactose at $70{^\circ}C$ and pH 7.0 was 134.9 U/mg. Consequently, this newly cloned enzyme appears to have a valuable advantage of conducting biotechnological processes at elevated temperature during milk pasteurization in the production of low-lactose milk.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7729-7735
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• 2015

As plastic injection mold parts is suitable system mass production making mold. So thick steering wheel parts is desirable to carry out gas injection molding. Gas injection mold is skill to inject nitrogen gas postfilling melting raw material into mold. Gas injection mold have many advantage like retrenchment of material cost, upgrading the guality. etc. It was decided gate position to minimize warpage of parts analysis injection mold process using mold flow software and incase doing gas injection mold using normal p.p material. it occur big warpage. so it is object minimizing warpage of injection parts to change p.p material containing mineral 18% and removing fingering phenomenon trouble as changing gate position. Also in case carrying out gas injection mold, I did comparison and analysis to grasp shape flow in gas setting a standard gate after flowing in raw material. Through this study, I found out changing of thickness by parts shape and it can occur warpage of parts by plastic material even though it carry out gas injection mold and it had a direct influence on trouble of parts by gate position.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.6 s.165
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• pp.879-891
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• 2007

Forecasting is a critical task for fashion companies because of continuous change in fashion and long process lead-time. Therefore, it is of great importance for both scholars and companies to understand how forecasted fashion styles are accepted by consumers. This research aimed to investigate consumer acceptance of fashion styles in Korea. The study examined and compared oversea collections of women's wear to the street fashion in Korea for seven seasons from 02 s/s to 05 s/s. Information on oversea collections were obtained from the magazine, Fashion Show, and the street fashion information from Seoul Fashion Design Center. The results showed that overall trends presented in oversea collections have been well accepted, while acceptance of specific styles or items varied. During the period of this research, sporty style and feminine style were very strong in the street fashion. Many styles and items were modified and selectively accepted probably because of cultural differences and limitations of mass production. Some styles which were presented in oversea collections were not accepted in Korea, and at the same time some cases were observed only in the street fashion in Korea. The results of this study provide guidelines for Korean apparel companies in merchandise planning and empirical findings to deepen the understanding on Korean society with respect to fashion.

• Proceedings of the KSEEG Conference
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• 2002.10a
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• pp.53-67
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• 2002

As in the past, we are concerned today with the magnitudes of mineral resources and the adequacy of these resources to meet future needs. In looking at global resource issues, we should consider the need for the resource, its supply, and the environmental consequences of using it. The need for a resource can become a resource dependency, specially as the global population expands and each of us becomes Increasingly dependent upon hundreds of natural materials. Therefore, our great mineral consumption makes the human population a true “Geologic Force”, which will be even more significant in the future when the global population is projected to reach alarming proportions. Although our supplies of mineral resources probably will be sufficient for the 21st century, the uneven distribution of minerals in the Earth's crust almost certainly will continue to be a major problem. The most likely result will be major shifts in both prices and sources of supply of many mineral resources. As for energy resources, we must avoid an obsessive dependency on one fuel and expand instead to other energy resources. Finally, because the use of resources affects the environment, we need to focus on resource exploitation and global pollution, particularly in regard to ground water and arable land. We must manage our resources so as to be in balance with our environment. And the accelerated industrialization of South Korean economy over the last three decades has resulted in the mass consumption of mineral commodities. South Korea has around 50 useful mineral commodities for the mineral industry, among 330 kinds of minerals described. The component ratio of the mining industry sector of the gross national production(GNP) in South Korea dropped from 1.2% in 1971 to 0.34% in 1997 due to the rapid growth of other industries in the country. During the period from 1971 to 1997, the average growth rate of mineral consumption in South Korea was 9.13% yearly and that of GMP per capita was 14.97%. The mineral consumptions per capita showed a continual increase during the last 30 years as follows(parenthesis: GW per capita); 0.99 metric tons in 1997($289), 3.83 metric tons in 1989($5, 210), 6.11 metric tons in 1995 ($10, 037), and 6.66 metric tons in 1997($9, 511). The total amount of mineral consumption in South Korea was 33 million tons of 32 mineral commodities in 1971, and 306 million metric tons of 47 mineral commodities in 1997.