• Journal of information and communication convergence engineering
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• 제18권3호
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• pp.188-193
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• 2020

In this paper, we examine whether the 3D image experience through a light-field display device showed the difference in the arousal of the user compared with the 2D image experience. For our experiment, the Looking Glass^TM (LG) was used as a lightfield display device that provided 3D images, and 2D images were provided by digital and printed images. The subject's facial behavior during each media experience was recorded for analysis and the degree of arousal was measured by FaceReader^TM. As a result, the first image presented in the first order among the three kinds of images showed that there was a statistical difference in the degree of arousal between the three media. However, no significant differences were found between the three media in the other images. This may be because the arousal did not increase from the experience of the second image through the LG, owing to habituation. In conclusion, the 3D imaging experience may appear in the beginning, but does not continue.

• BMB Reports
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• 제47권12호
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• pp.691-696
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• 2014

${\alpha}$-Enolase is a glycolytic enzyme and a surface receptor for plasminogen. ${\alpha}$-Enolase-bound plasminogen promotes tumor cell invasion and cancer metastasis by activating plasmin and consequently degrading the extracellular matrix degradation. Therefore, ${\alpha}$-enolase and plasminogen are novel targets for cancer therapy. We found that the amino acid sequence of a peptide purified from enzymatic hydrolysates of seahorse has striking similarities to that of ${\alpha}$-enolase. In this study, we report that this peptide competes with cellular ${\alpha}$-enolase for plasminogen binding and suppresses urokinase plasminogen activator (uPA)-mediated activation of plasminogen, which results in decreased invasive migration of HT1080 fibrosarcoma cells. In addition, the peptide treatment decreased the expression levels of uPA compared to that of untreated controls. These results provide new insight into the mechanism by which the seahorse-derived peptide suppresses invasive properties of human cancer cells. Our findings suggest that this peptide could emerge as a potential therapeutic agent for cancer.

• Animal cells and systems
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• 제4권4호
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• pp.341-345
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• 2000

It has been very difficult to develop and evaluate efficient fish vaccines because fish immune cells have not been properly characterized. In this study, we investigated the cell-mediated immunological properties of B- and T-like cells in Nile tilapia (Oreochromis nilotica). Surface immunoglobulin negative ($slg^{-}$) cell population proliferated in response to mammalian T-cell mitogens PHA and Con A, while surface immunoglobulin positive ($slg^{+}$) cells responded to the B-cell mitogen LPS. The slg$^{[-10]}$ cells from hemocyanin (HC)-immunized Tilapia, compared to the non-immunized control, reacted more to PHA than to Con A. Unexpectedly, antigen (Ag)-specific response was observed in both $slg^{-}$ and $slg^{-}$cells. Regardless of HC immunization, whole leukocytes from 8 head kidney of fish showed natural killer (NK)cell activity. Especially, NK cell activity was much higher in slg$^{[-10]}$ cells than in slg$^{+}$cells, indicating the possibility that fish NK cells were not at least associated with slg$^{+}$ cell population and not activated by Ag. Further understanding of functional fish immune cells will help to evaluate and develop effective vaccines for fishes and to monitor the course of therapy In infected fishes.hes.

• 한국해양바이오학회지
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• 제2권1호
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• pp.48-54
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• 2007

바실러스 폴리퍼멘티쿠스 에스씨디는 활성아포를 형성하여 장까지 도달하는 특성 때문에 장기적인 장 질환 치료에 사용되어져 왔다. 뿐만 아니라 강력한 항생물질을 생산하는 장점을 가지고 있다. 현 연구는 바실러스 폴리퍼멘티쿠스 에스씨디와 콩을 함께 발효시킨 것 (BPFS)과 그것의 생균제 특성에 관하여 조사하였다. 바실러스 폴리퍼멘티쿠스 에스씨디는 와 BPFS는 병원성 그람양성 (스트렙토코커스 파라우베리스, 스트렙토코커스 이니에, 락도코커스 가르비에), 그람 음성 (플렉시박터 트렉튜오서스, 비브리오 하베이, 비브리오 불니피쿠스, 비브리오 오다리), 곰팡이 (아스파라질러스 니거, 아스파라질러스 오리자에)에 이르기까지 넓은 범위의 항균성을 가졌다. BPFS에 의한 생존율 증가와 부영양화로 인한 수질오염의 정화작용을 관찰하기 위해서 통영 앞바다 양식장에서 조피볼락을 사용해서 현장 실험을 하였다. 그 결과, 해양 병원성 유해 박테리아에 노출시키고 일반 사료만 복용시킨 군에서는 99%의 폐사율을 보였으나, BPFS를 사료와 함께 복용시킨 군에서는 먹이 유인효과에 의한 성장률의 증가와 부영양화에 의한 수질오염을 정화하는 작용이 관찰되었다. 따라서 BPFS는 양식업에서 항생물질 대체제, 사료첨가제, 부영양화에 의한 정화제로서 개발하여 산업화하기 용이한 후보물질이라고 사료된다.

• Nutrition Research and Practice
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• 제12권3호
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• pp.199-207
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• 2018

BACKGROUND/OBJECTIVES: Fermented Laminaria japonica (FL), a type sea tangle used as a functional food ingredient, has been reported to possess cognitive improving properties that may aid in the treatment of common neurodegenerative disorders, such as dementia. MATERIALS/METHODS: We examined the effects of FL on scopolamine (Sco)- and ethanol (EtOH)-induced hippocampus-dependent memory impairment, using the Passive avoidance (PA) and Morris water maze (MWM) tests. To examine the underlying mechanisms associated with neuroprotective effects, we analyzed acetylcholine (ACh) and acetylcholinesterase (AChE) activity, brain tissue expression of muscarinic acetylcholine receptor (mAChR), cAMP response element binding protein (CREB) and extracellular signal-regulated kinases 1/2 (ERK1/2), and immunohistochemical analysis, in the hippocampus of mice, compared to current drug therapy intervention. Biochemical blood analysis was carried out to determine the effects of FL on alanine transaminase (ALT), aspartate transaminase (AST), and triglyceride (TG) and total cholesterol (TC) levels. 7 groups (n = 10) consisted of a control (CON), 3 Sco-induced dementia and 3 EtOH-induced dementia groups, with both dementia group types containing an untreated group (Sco and EtOH); a positive control, orally administered donepezil (Dpz) (4mg/kg) (Sco + Dpz and EtOH + Dpz); and an FL (50 mg/kg) treatment group (Sco + FL50 and EtOH + FL50), orally administered over the 4-week experimental period. RESULTS: FL50 significantly reduced EtOH-induced increase in AST and ALT levels. FL50 treatment reduced EtOH-impaired step-through latency time in the PA test, and Sco- and EtOH-induced dementia escape latency times in the MWM test. Moreover, anticholinergic effects of Sco and EtOH on the brain were reversed by FL50, through the attenuation of AChE activity and elevation of ACh concentration. FL50 elevated ERK1/2 protein expression and increased p-CREB (ser133) in hippocampus brain tissue, according to Western blot and immunohistochemistry analysis, respectively. CONCLUSION: Overall, these results suggest that FL may be considered an efficacious intervention for Sco- and EtOH-induced dementia, in terms of reversing cognitive impairment and neuroplastic dysfunction.

• 한국운동역학회지
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• 제21권4호
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• pp.421-427
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• 2011

The purpose of this study was to analyze the difference between male and female tennis players' two-handed backhand stroke and to provide basic data which helps precise and efficient instruction for the sake of precise postures, enhanced performances and skills. 5 male and 5 female university players were recruited as subjects, and the mean difference between the kinematic variables such as the time from backswing to impact and total swing time, racket head velocity, change of the center of body gravity in two-handed backhand stroke through three-dimensional motion analysis. The test data was analyzed by t-test, and the alpha level of ${\alpha}$=.05 was set for all tests of significance. The findings of the study were as follows; First, there was no difference in the time from backswing to impact and total time of

• Biomolecules & Therapeutics
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• 제26권6호
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• pp.539-545
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• 2018

4-Hydroxy-2-(4-hydroxyphenethyl)isoindoline-1,3-dione (PD1) is a synthetic phthalimide derivative of a marine compound. PD1 has peroxisome proliferator-activated receptor (PPAR) ${\gamma}$ agonistic and anti-inflammatory effects. This study aimed to investigate the effect of PD1 on allergic asthma using rat basophilic leukemia (RBL)-2H3 mast cells and an ovalbumin (OVA)-induced asthma mouse model. In vitro, PD1 suppressed ${\beta}$-hexosaminidase activity in RBL-2H3 cells. In the OVA-induced allergic asthma mouse model, increased inflammatory cells and elevated Th2 and Th1 cytokine levels were observed in bronchoalveolar lavage fluid (BALF) and lung tissue. PD1 administration decreased the numbers of inflammatory cells, especially eosinophils, and reduced the mRNA and protein levels of the Th2 cytokines including interleukin (IL)-4 and IL-13, in BALF and lung tissue. The severity of inflammation and mucin secretion in the lungs of PD1-treated mice was also less. These findings indicate that PD1 could be a potential compound for anti-allergic therapy.

• 한국수산과학회지
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• 제46권5호
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• pp.528-533
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• 2013

This study evaluated the impact of antimicrobial resistant bacteria produced by inland pollution sources on coastal areas, and investigates antimicrobial resistance profiles of bacteria isolated from marine filter feeders. A total of 107 shellfish (short-necked clams Ruditapes philippinarum, ark shells Scapharca broughtonii, and oysters Crassostrea gigas) were collected from the southern coast of Korea, from which 204 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 15 antimicrobial agents used in Korea for clinical or veterinary therapy. Antimicrobial resistance was present in 44.6% of E. coli isolates against at least one antimicrobial agent. The rate of antimicrobial resistance in the Narodo area was higher than isolates from the Gangjinman area and Kamak Bay. E. coli isolates had a higher rate of resistance against: tetracycline (29.9%), streptomycin (25.5%), and trimethoprim (14.2%). Of 204 isolates, 29 (14.2%) were resistant to multiple antimicrobial agents.

• 한국자원식물학회지
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• 제24권3호
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• pp.298-303
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• 2011

A large number of edible seaweeds are consumed by the coastal peoples of Asia. Some of them are used in traditional remedies in many parts of the world. In this study we investigated effects of supplementation with ethyl acetate extracts of the brown alga Eisenia bicyclis (EBE) on rat macrophage to evaluate the possibilities as immune-modulators. Twelve male SD rats were divided into two groups and the treatments were as follows: A, no Eisenia bicyclis extract (EBE) intake and distilled water ; B, oral supplemented with EBE 200 mg/kg. After 5 weeks of supplementation, rats were sacrificed to assess the effect on peritoneal macrophage functions. We showed no increasing effects on tumoricidal activity, phagocytic activity and NO production in macrophages in EBE supplementation group. However, EBE supplementation suppressed NO-iNOS production and p65 translocation into the nucleus in LPS-stimulated macrophages. Overall, these results suggest that the supplementation of EBE might have an anti-inflammatory effects on NO-iNOS production in macrophages throughout the inhibition of NF-${\kappa}B$ activation.

• 한국방사선학회논문지
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• 제14권4호
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• pp.415-424
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• 2020

본 연구는 부산시내에 있는 1차, 2차, 3차 병원에 근무하는 방사선사들을 대상으로 조직몰입을 측정하고, 직무만족 및 직무스트레스가 조직몰입에 미치는 영향에 대해 알고자 설문조사를 실시하고 분석하였다. 그 결과 3차 병원의 직무만족과 조직유효성의 점수가 통계적으로 유의하게 높게 나타났다(p<0.05). 이직경험이 없는 그룹이 조직몰입, 직무만족 및 조직유효성의 점수가 통계적으로 유의하게 높게 나타났다(p<0.05, p<0.01). 직무스트레스는 일반적인 특성에 따른 분석에서 변수 간 통계적으로 유의한 차이는 없었다. 위계적 회귀분석 결과 방사선사의 조직몰입이 직무만족에 가장 높은 영향력을 미치는 것으로 나타났다(β=0.703, p<0.001). 방사선사는 병원 조직 안에서 질병의 진단 및 치료에 있어서 중요한 위치에 있다. 따라서 본 연구의 결과를 바탕으로 방사선사의 직무만족 및 직무스트레스와 조직몰입과의 관계를 분석하여 방사선사들의 조직 몰입을 높일 수 있는 방안을 마련하는데 도움이 될 것으로 사료된다.