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http://dx.doi.org/10.7732/kjpr.2011.24.3.298

Immunomodulatory Effects of Supplementation with Extracts from the Marine Brown Alga Eisenia bicyclis on Macrophages  

NamKoong, Seung (Department of Physical Therapy, Kangwon National University)
Kang, Se-Chan (Department of Natural Medicine Resources, Semyung University)
Do, Hang (College of Pharmacy, Sungkyunkwan University)
Jang, Ki-Hyo (Department of Food and Nutrition, Kangwon National University)
Jang, Seon-A (Department of Herbal Medicine Resource, Kangwon National University)
Choung, Myoung-Gun (Department of Herbal Medicine Resource, Kangwon National University)
Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.24, no.3, 2011 , pp. 298-303 More about this Journal
Abstract
A large number of edible seaweeds are consumed by the coastal peoples of Asia. Some of them are used in traditional remedies in many parts of the world. In this study we investigated effects of supplementation with ethyl acetate extracts of the brown alga Eisenia bicyclis (EBE) on rat macrophage to evaluate the possibilities as immune-modulators. Twelve male SD rats were divided into two groups and the treatments were as follows: A, no Eisenia bicyclis extract (EBE) intake and distilled water ; B, oral supplemented with EBE 200 mg/kg. After 5 weeks of supplementation, rats were sacrificed to assess the effect on peritoneal macrophage functions. We showed no increasing effects on tumoricidal activity, phagocytic activity and NO production in macrophages in EBE supplementation group. However, EBE supplementation suppressed NO-iNOS production and p65 translocation into the nucleus in LPS-stimulated macrophages. Overall, these results suggest that the supplementation of EBE might have an anti-inflammatory effects on NO-iNOS production in macrophages throughout the inhibition of NF-${\kappa}B$ activation.
Keywords
Eisenia bicyclis; iNOS; Macrophage; Nitric oxide; p65;
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1 Okimura, T., M. Ogawa and T. Yamauchi. 1986. Stress and immune responses. III. Effect of resistant stress on delayed type hypersensitivity (DTH) response, natural killer (NK) activity and phagocytosis in mice. Japanese J. Pharmacol. 41: 229-235.   DOI
2 Speziali, E., A.F. Santiago, R.M. Fernandes, N.M. Vaz, J.S. Menezes and A.M. Faria. 2009. Specific immune responses but not basal functions of B and T cells are impaired in aged mice. Cell. Immunol. 256:1-5.   DOI
3 Verstovsek, S., D.L. Maccubbin, M.J. Ehrke and E. Mihich. 1992. Tumoricidal activation of murine resident peritoneal macrophages by interleukin-2 and tumor necrosis factor. Cancer Res. 52:3880-3885.
4 Kang, K., H. J. Hwang, D. H. Hong, Y. Park, S. H. Kim, B. H. Lee and H. C. Shin. 2004. Antioxidant and antiinflammatory activities of ventol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture. Res. Commun. Mol. Pathol. Pharmacol. 115-116:77-95.
5 Barnes, P.J. 2008. Role of GATA-3 in allergic diseases. Curr. Mol. Med. 8:330-334.   DOI   ScienceOn
6 Bredt, D.S. and S.H. Snyder. 1994. Nitric oxide: a physiologic messenger molecule. Annu. Rev. Biochem. 63:175-195.   DOI   ScienceOn
7 Ding, A.H., C.F. Nathan and D.J. Stuer. 1988. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141:2407-2412.
8 Fidler, I.J. and E.S. Kleinerman. 1983. Therapy of cancer metastasis by systemic activation of macrophages: from the bench to the clinic. Res. Immunol. 144:284-287.
9 Hahn, H. and S.H. Kaufmann. 1981. The role of cell mediated immunity in bacterial infections. Rev. Infect. Dis. 3:1221-1250.   DOI
10 Yang, L., L. Cohn, D.H. Zhang, R. Homer, A. Ray and P. Ray P. 1998. Essential role of nuclear $factor-{\kappa}B$ in the induction of eosinophilia in allergic airway inflammation. J. Exp. Med. 188:1739-1750.   DOI   ScienceOn
11 Hemmi, H. and N. Ishida. 1980. The immune response of splenic lymphocytes after cimicifugoside treatment in vitro and pretreatment in vivo. J. Pharmacobiodyn. 3:643-648.   DOI
12 Karin, M. and A. Lin. 2002. $NF-{\kappa}B$ at the crossroads of life and death. Nat. Immunol. 3:221-227.
13 Keller, R. and R. Keist. 1989. Abilities of activated macrophages to manifest tumoricidal activity and to generate reactive nitrogen intermediates: a comparative study in vitro and ex vivo. Biochem. Biophys. Res. Commun. 164:968-973.   DOI   ScienceOn
14 Keller, R., R. Keist and K. Frei. 1990. Lymphokines and bacteria that induce tumoricidal activity, trigger a different secretory response in macrophages. Eur. J. Immunol. 20:695-698.   DOI   ScienceOn
15 Hibbs, J.B. Jr., R.R. Taintor and Z. Vavrin. 1987. Macrophage cytotoxicity: role for L-arginine deaminase and imino nitrogen oxidation to nitrite. Science 235:473-476.   DOI
16 Hibbs, J.B. Jr,, R.R. Taintor, Z. Vavrin and E.M. Rachlin. 1998 Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157:87-94.
17 Hu, T., D. Liu, Y. Chen, J. Wu and S. Wang. 2010. Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro. Int. J. Biol. Macromol. 46:193-198.   DOI   ScienceOn
18 Klimp, A.H., E.G. de Vries, G.L. Scherphof and T. Daemen. 2002. A potential role of macrophage activation in the treatment of cancer. Crit. Rev. Oncol. Hematol. 44:143-161.   DOI   ScienceOn
19 Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63.   DOI
20 Nathan, C.F. 1987. Secretory products of macrophages. J. Clin. Invest. 79:319-326.   DOI   ScienceOn
21 Joe, M.J., S.N. Kim, H.Y. Choi, W.S. Shin, G.M. Park, D.W. Kang and Y.K. Kim. 2006. The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts. Biol. Pharm. Bull. 29:1735-1739.   DOI   ScienceOn
22 Adams, D.O. and T.A. Hamilton. 1984. The cell biology of macrophage activation. Annu. Rev. Immunol. 2:283-318.   DOI   ScienceOn