• Title/Summary/Keyword: marine photobioreactor

Search Result 18, Processing Time 0.022 seconds

Enhancing Photon Utilization Efficiency for Astaxanthin Production from Haematococcus lacustris Using a Split-Column Photobioreactor

  • Kim, Z-Hun;Park, Hanwool;Lee, Ho-Sang;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
    • /
    • v.26 no.7
    • /
    • pp.1285-1289
    • /
    • 2016
  • A split-column photobioreactor (SC-PBR), consisting of two bubble columns with different sizes, was developed to enhance the photon utilization efficiency in an astaxanthin production process from Haematococcus lacustris. Among the two columns, only the smaller column of SC-PBR was illuminated. Astaxanthin productivities and photon efficiencies of the SC-PBRs were compared with a standard bubble-column PBR (BC-PBR). Astaxanthin productivity of SC-PBR was improved by 28%, and the photon utilization efficiencies were 28-366% higher than the original BC-PBR. The results clearly show that the effective light regime of SC-PBR could enhance the production of astaxanthin.

Effect of Liquid Circulation Velocity and Cell Density on the Growth of Parietochloris incisa in Flat Plate Photobioreactors

  • Changhai Wang;Yingying Sun;Ronglian Xing;Liqin Sun
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.10 no.2
    • /
    • pp.103-108
    • /
    • 2005
  • For more accurately describing the durations of the light and the dark phases of micro-algal cells over the whole light-dark cycle, and probing into the relationship between the liquid circulation time or velocity, the aeration rate and cell density, a series of experiments was carried out in 10 cm light-path flat plate photobioreactors. The results indicated that the liquid flow in the flat plate photobioreactor could be described by liquid dynamic equations, and a high biomass output, higher content and productivity of arachidonic acid, $70.10\;gm^{-2}d^{-1},\;9.62\%$ and 510.3 mg/L, respectively, were obtained under the optimal culture conditions.

Comparison of Biomass Productivity of the Microalgae, Tetraselmis sp. KCTC12236BP, in Polyvinyl Chloride Marine Photobioreactor and High Density Polyethylene Marine Photobioreactor (폴리비닐클로라이드 해양광생물반응기와 고밀도 폴리에틸렌 해양광생물반응기에서 미세조류, Tetraselmis sp. KCTC12236BP의 생산성 비교)

  • Jung, Seung-Gyun;Kim, Su-Kwon;Bun, Moon-Sup;Cho, Yonghee;Shin, Dong-Woo;Kim, Z-Hun;Lim, Sang-Min;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
    • /
    • v.8 no.1
    • /
    • pp.18-23
    • /
    • 2016
  • It is important to design photobioreactor by cheap material for economical microalgal biomass production. In this study, two types of marine photobioreactors (MPBR), made by either polyvinyl chloride (MPBR-PVC) or high density poly ethylene (MPBR-HDPE), are used and performance of these were compared. Tetraselmis sp. KCTC 12236BP is a green marine alga that isolated from Ganghwa Island, Korea, and the strain was used for marine cultivations using MPBR-PVC and MPBR-HDPE. The cultivations were performed three times in the spring season of 2012 using MPBR-PVC and of 2013 using MPBR-HDPE in the coastal area of Young Heung Island. As the results, MPBR-PVC shows higher biomass productivities than MPBR-HDPE, due to its high light transmittance. In the cultivations using MPBR-PVC, the average sea water temperature was $11.5^{\circ}C$ during the first experiment and $16.5^{\circ}C$ during the second and third experiments. Average light intensities during three times for experiments were 407.5, 268.1 and $273.0{\mu}{\cdot}E{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. The maximum fresh cell weight and average biomass productivity were $1.2g{\cdot}L^{-1}$ and $0.12g{\cdot}L^{-1}{\cdot}day^{-1}$. These results showed that Tetraselmis sp. KCTC12236BP were adapted well with the environmental conditions from ocean, and grow in the MPBR-PVC and MPBR-HDPE.

Improvement of Unsaturated Fatty Acid Production from Porphyridium cruentum Using a Two-Phase Culture System in a Photobioreactor with Light-Emitting Diodes (LEDs)

  • Kim, So Hee;Lee, Ui Hun;Lee, Sang Baek;Jeong, Gwi-Taek;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
    • /
    • v.31 no.3
    • /
    • pp.456-463
    • /
    • 2021
  • In this study, the culture conditions for Porphyridium cruentum were optimized to obtain the maximum biomass and lipid productions. The eicosapentaenoic acid content was increased by pH optimization. P. cruentum was cultured with modified F/2 medium in 14-L photobioreactors using a two-phase culture system, in which the green (520 nm) and red (625 nm) light-emitting diodes (LEDs) were used during the first and second phases for biomass production and lipid production, respectively. Various parameters, including aeration rate, light intensity, photoperiod, and pH were optimized. The maximum biomass concentration of 0.91 g dcw/l was obtained with an aeration rate of 0.75 vvm, a light intensity of 300 μmol m-2s-1, and a photoperiod of 24:0 h. The maximum lipid production of 51.8% (w/w) was obtained with a light intensity of 400 μmol m-2s-1 and a photoperiod of 18:6 h. Additionally, the eicosapentaenoic acid and unsaturated fatty acid contents reached 30.6% to 56.2% at pH 6.0.

Verification of a Relationship between Ultraviolet Radiation and Initial Microalgal Cell Density Using a Floating Marine Photobioreactor (부유형 해양 광생물반응기를 이용한 자외선과 초기 미세조류 접종 농도와의 상관관계 규명)

  • Kim, Z-Hun;Park, Hanwool;Jung, Seong-Gyun;Kim, Su-Kwon;Kim, Hee-Yun;Park, Yong Sung;Hong, Han Ma Roo;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
    • /
    • v.7 no.2
    • /
    • pp.52-57
    • /
    • 2015
  • The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off film were placed in an outdoor rectangular tank containing 200 L of water. At the lower ICDs, 0.01 and 0.05 g/L, biomass productivities in the PBRs without UV cut-off film decreased by $278{\pm}21%$ and $222{\pm}3%$ compared with those with the film, respectively. In contrast, the presence of UV cut-off film did not have a significant effect on biomass productivities at the higher ICDs, 0.25 and 1.25 g/L. When the differences in biomass productivity made by the UV cut-off film were plotted against the sum of cell projection area per light receiving area of the PBR, the results revealed that the inhibitory effect of UV on biomass productivity can be negligible when the sum of cell projection area is equal to the light receiving area of the PBR. These results show that photoinhibition caused by UV radiation could be eliminated via operating the PBR with a proper ICD.

Optimal Culture Conditions for Marine Chlorella in a Vertical Tubular Photobioreactor System (해수산 Chlorella의 최적 배양 조건에 관한 연구)

  • LIM Jin-Young;CHO Man-Gi;HAN Bong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.31 no.1
    • /
    • pp.139-142
    • /
    • 1998
  • To obtain informations for construction of a mass culture system, factors affecting on the specific growth rate of marine Chlorella sp. purchased from the Chungmu Laboratory of the South Sea Fisheries Institute, the National Fisheries Research and Development Agency were investigated using a vertical tubular photobioreactor (VT-PBR) system. Optimal temperature, illumination intensity, air- and $CO_{2-}$ flow rate for Chlorella sp. were $20^{\circ}C$, 6,000 lux, 0,56 vvm and 0.028 vvm, respectively.

  • PDF

Specific Growth Rates of Microalgae in Different Types of Model Photobioreactors (모형 배양조 형태에 따른 단세포 조류의 비증식속도)

  • KWAK Jung-Ki;KIM Hyun-Ju;LEE Ji-Hyun;SHIN Ga-Hee;CHO Man-Gi;HAN Bong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.31 no.4
    • /
    • pp.477-482
    • /
    • 1998
  • In the aquaculture industry, a photobioreactor (Pbr) with high productivity is a prerequisite for mass production of Chlorella sp., a feeding fry for Rotifer (Brachinous plicatilis). To enhance the productivity of Chlorella sp., model Pbrs such as Cylinder type, Spherical surface type, Half-spherical surface type, Plate type, Raceway pond type and Water-wheel type Pbr with different values of surface area exposed to light/culture volume (S/V) were manufactured, and the maximum specific growth rate (${\mu}_{max}$) and productivity of Chlorella vulgaris 211-11b at $25^{\circ}C$, pH 7.0 and 12,000 lux were compared each other. The ${\mu}_{max}$ and productivity were not proportional to S/V. Among the 6 model Pbrs, Half-spherical surface type Pbr showed the highest ${\mu}_{max}$ and productivity as 2.206 ($day^{-1}$) and 0.247($g^{{\ell}-1}day^{-1}$).

  • PDF

Seasonal Assessment of Biomass and Fatty Acid Productivity by Tetraselmis sp. in the Ocean Using Semi-Permeable Membrane Photobioreactors

  • Kim, Z-Hun;Park, Hanwool;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
    • /
    • v.26 no.6
    • /
    • pp.1098-1102
    • /
    • 2016
  • A green microalga, Tetraselmis sp., was cultivated in the coastal seawater of Young-Heung Island using semi-permeable membrane photobioreactors (SPM-PBRs) in different seasons. The microalgae in the SPM-PBRs were able to grow on nutrients diffused into the PBRs from the surrounding seawater through SPMs. The biomass productivity varied depending on the ion permeabilities of the SPMs and environmental conditions, whereas the quality and quantity of fatty acids were constant. The temperature of seawater had a greater influence than solar radiation did on productivity of Tetraselmis sp. in SPM-PBRs. SPM-PBRs could provide technologies for concurrent algal biomass and fatty acids production, and eutrophication reduction in the ocean.

Content of Phycocyanins and Growth of Spirulina platensis with Culture Conditions (배양 조건에 따른 Spirulina piatensis의 성장 및 phycocyanin 함량 변화)

  • JOO Dong Sik;JUNG Chung Kuk;LEE Chang Ho;CHO Soon Yeong
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.33 no.5
    • /
    • pp.475-481
    • /
    • 2000
  • The growth of the microalgal Spiulina platensis in a batch photobioreactor had been studied to determine the influence of temperature, light intensity, and culture medium on the growth and c-phycocyanin content of the biomass. The most favorable conditions for high biomass and c-phycocyanin production were as follows: light intensity of 3500 lux, temperature of $35^{\circ}C,\;NaHCO_3\;of\;1.0{\%}$ for pH control, $0.2{\~}0.3{\%}\;Na_2CO_3$ for carbon source, and $0.2{\~}0.3{\%}\;NaCO_3$ for nitrogen source. The c-phycocyanin and chlorophyll content on most favorable condition were about $11{\%},\;1.0{\%}$, respectively.

  • PDF

Enhanced Biomass Productivity of Freshwater microalga, Parachlorella kessleri for Fixation of Atmospheric CO2 Using Optimal Culture Conditions (최적 배양 조건을 이용한 CO2 제거 목적의 담수 미세조류 Parachlorella kessleri의 바이오매스 생산성 향상)

  • Z-Hun Kim;Sun Woo Hong;Jinu Kim;Byungrak Son;Mi-Kyung Kim;Yong Hwan Kim;Jin Hyun Seol;Su-Hwan Cheon
    • Journal of Marine Bioscience and Biotechnology
    • /
    • v.16 no.1
    • /
    • pp.36-44
    • /
    • 2024
  • This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO2. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO2 fixation by increasing the availability of CO2(HCO3-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m2/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO2 availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO2 fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.