• Title/Summary/Keyword: marine bio

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Gross Abnormalities in Tilapia

  • Tave, Douglas;Jo, Jae-Yoon;Kim, Dong-Soo
    • Fisheries and Aquatic Sciences
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    • v.14 no.2
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    • pp.148-160
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    • 2011
  • Thirty-one gross abnormalities that have been observed in tilapia are described: 10 fin, five eye, five jaw, four body shape, three head, two yolk sac, one operculum, and conjoined twins. Twenty-one have been described in published papers; the others were obtained from a survey. Breeding experiments revealed that three were heritable, while six were not heritable. Five could be caused by a bacterial infection, and one could be produced by a fungus. Four deformities were in offspring of males that had been injected with methyl methane sulphonate. Three were produced when sperm was treated with methyl methane sulphonate. Six were observed during sex reversal studies, and one was found following heat shock of fertilized eggs. Three were observed in polluted river water. The cause of other deformities is not known.

Development of Biorefinery Process using Microalgae (미세조류를 이용한 바이오리파이너리 공정의 개발)

  • Shin, Hyun-Jae;Park, Ju-Hyun;Jung, Won-Kyo;Cho, Hoon;Kim, Si-Wouk
    • Journal of the Korean Society for Precision Engineering
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    • v.28 no.2
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    • pp.154-167
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    • 2011
  • Recently, microalgae have been focused on potential biomass for bio-diesel and biorefinery process. The aim of this paper is to review the biorefinery process including biodesel using microalgae as a microreactor. The state-of-the-art of biodiesel and biorefinery research such as extraction and reaction process as well as byproducts utilization is described. In addition, we suggest possibility for develop bioactive substances and their industrial products from byproducts of microalgae massively obtained after bio-diesel extraction.

Purification and Characterization of Extracellular Protease form Psychrotrophic Antarctic Bacteria (남극에서 분리한 저온성 세균 유래 단백질 분해 효소)

  • 조기웅;방지헌;홍혜원;박승일;이윤호
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.254-259
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    • 2002
  • A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.

Antioxidant and Antibacterial Activities of Chitosan-Phloroglucinol Conjugate

  • Lee, Dae-Sung;Cho, Young-Sook;Je, Jae-Young
    • Fisheries and Aquatic Sciences
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    • v.16 no.4
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    • pp.229-235
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    • 2013
  • In an effort to develop biopolymer-based antioxidant and antibacterial materials, a chitosan-phloroglucinol conjugate was prepared and cellular antioxidant activity and minimum inhibitory concentration against foodborne pathogens and methicillin-resistant Staphylococcus aureus (MRSA) evaluated. The chitosan-phloroglucinol conjugate showed higher antioxidant activities than the unmodified chitosan (P < 0.05). The chitosan-phloroglucinol conjugate showed 62.29% reactive oxygen species scavenging activity, 56.11% lipid peroxidation inhibition activity, and 2.21-fold increase of glutathione expression in mouse macrophage cells. Additionally, the chitosan-phloroglucinol conjugate exhibited higher antibacterial activities than the unmodified chitosan, and the chitosan-phloroglucinol conjugate showed fourfold higher antibacterial activities against MRSA and clinical isolates and twofold higher activities against foodborne pathogens compared to the unmodified chitosan.

Hot water extract of Loliolus beka attenuates methylglyoxal-induced advanced glycation end products formation in human umbilical vein endothelial cells

  • Cha, Seon-Heui;Jun, Hee-Sook
    • Fisheries and Aquatic Sciences
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    • v.25 no.10
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    • pp.517-524
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    • 2022
  • Over production of methylglyoxal (MGO) a highly reactive dicarbonyl compound, has been associated in progressive diabetes with vascular complication. Therefore, we investigated whether hot water extract of Loliolus beka meat (LBM-HWE) presents a preserve effect against MGO-induced cellular damage in human umbilical vein endothelial cells (HUVECs). The LBM-HWE extract showed to inhibit MGO-induced cytotoxicity. Additionally, the LBM-HWE reduced mRNA expression of pro-inflammatory cytokines, and reduced MGO-induced advanced glycation end product (AGEs) formation. Furthermore, LBM-HWE induced glyoxalase-1 mRNA expression and reduced MGO-induced carbonyl protein formation in HUVECs. The results implicate that LBM-HWE has protective ability against MGO-induced HUVECs toxicity by preventing AGEs formation. In conclusion, LBM-HWE could be used as a potential treatment material for the prevention of vascular complications of diabetes.

Long arm octopus (Octopus minor) extract prevents eye injury caused by particulate matter exposure in zebrafish (Danio rerio) embryos

  • Thilini Ranasinghe;Seon-Heui Cha
    • Fisheries and Aquatic Sciences
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    • v.27 no.2
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    • pp.111-121
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    • 2024
  • Particulate matter (PM) is a mixture of microscopic solid inhalable particles including airborne liquid droplets, and it is implicated with several diseases. The eye does not have a protective barrier among the human organs, consequently it get directly exposed to environmental substances such as PM. The scarcity of treatments for damage to the eyesight and the vision and eye structure being closely related to the structure and function of the central nervous system highlights the cruciality of novel therapeutics. In this study was conducted using in vivo zebrafish vertebrate model which is a useful model due to the conserved genes between human. We found that protective effect of Octopus minor extract against particulate matter-induced adverse effects on eye development in zebrafish (Danio rerio) embryos by regulating antioxidant and anti-inflammatory mRNA expression.

Trends in the Korean Government Support for Marine Biotechnology R&D Investment and Its Implication (해양바이오 분야 정부 R&D 지원사업의 동향과 함의)

  • Jang, Duckhee;Doh, Soogwan
    • Ocean and Polar Research
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    • v.40 no.3
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    • pp.177-190
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    • 2018
  • This study seeks to analyze trends in Korean government support for marine biotechnology R&D investment. The quantitative analysis proposed in this paper provides useful data to inform government policy making decisions in relation to R&D investment in the marine biotechnology. Data used in this study is from NTIS (National Science & Technology Information Service) DB. Empirical results indicate that national R&D investment in the area of marine biotechnology has recently been increased in terms of the number of R&D programs and the amount of R&D investment. The results also show that the R&D programs in the area of marine biotechnology involve various Ministries of the government of Korea, although the Ministry of Oceans and Fisheries (MOF) performed a key role in the R&D investment in the marine biotechnology. Finally, the results of social network analysis in this study reveals that most of the R&D programs in the area of marine biotechnology seeks the development of new materials using unique features of marine bioresources.

Spatial distribution of dinoflagellate resting cysts in Yellow Sea surface sediments

  • Hwang, Choul-Hee;Kim, Keun-Yong;Lee, Yoon;Kim, Chang-Hoon
    • ALGAE
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    • v.26 no.1
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    • pp.41-50
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    • 2011
  • Yellow Sea surface sediment samples collected on October 15-31, 2003 were analyzed using the palynological process to investigate the spatial distribution of dinoflagellate resting cysts. The sampling areas comprised four latitudinal transects, the northernmost of which was located off the Shandong Peninsula, China and the southernmost off Jeju Island, Korea. Each transect line was composed of six to nine stations, spanning the distance between the Chinese and Korean coasts. Twenty-five different types of dinoflagellate cysts were identified. Gonyaulax scrippsae, Alexandrium spp. (ellipsoidal type), and G. spinifera were the most dominant at all stations surveyed. Dinoflagellate cysts belonging to the Gonyaulacales comprised over 50% of all cysts collected. The latitudinal distribution trend showed that cyst concentrations along the two middle transects were much higher than those along the two northern and southern transects. Cyst concentrations in the offshore central areas reached their highest values within each transect and gradually decreased toward the Chinese and Korean coasts. Overall, cyst concentrations were markedly elevated in the offshore central Yellow Sea areas and gradually decreased outward in all four directions. This concentric cyst distribution pattern was consistent with the hydrographic features of the Yellow Sea, such as circular current systems, sedimentary properties, and water depth.

Characterization and expression profiles of aquaporins (AQPs) 1a and 3a in mud loach Misgurnus mizolepis after experimental challenges

  • Lee, Sang Yoon;Nam, Yoon Kwon;Kim, Yi Kyung
    • Fisheries and Aquatic Sciences
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    • v.20 no.9
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    • pp.23.1-23.9
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    • 2017
  • Two distinct cDNAs encoding aquaporins (mmAQPs 1a and 3a) were isolated and characterized from mud loach Misgurnus mizolepis. The identified mud loach AQP cDNAs encode for polypeptides of 260 and 302 amino acids. Topology predictions confirmed six putative membrane-spanning domains connected by five loops and the N- and C-terminal domains being cytoplasmic. The mud loach AQPs 1a and 3a showed broad distribution in multiple tissues including immune-responsive tissues as well as osmoregulatory tissues. Hence, the diversity of AQP distribution and expression possibly indicated its differential functions in the regulation of fluid movement in response to environmental stimuli. The transcription of mmAQP genes was differentially modulated by immune challenges. In particular, the mmAQP3a expression level in the liver was more responsive to immune challenges than that of mmAQP1a. Taken together, fish stimulation or infection resulted in significant modulation of mud loach AQP genes, suggesting potential functional roles of these proteins in piscine pathophysiological process.

Effects of Recombinant Aquaporin 3 and Seawater Acclimation on the Expression of Aquaporin 3 and 8 mRNAs in the Parr and Smolt Stages of Rainbow Trout, Oncorhynchus mykiss

  • Kim, Na Na;Choi, Young Jae;Lim, Sang-Gu;Kim, Bong-Seok;Choi, Cheol Young
    • Ocean and Polar Research
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    • v.38 no.2
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    • pp.103-113
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    • 2016
  • This study aimed to examine the role of two aquaporin isoforms (AQP3 and AQP8) in response to the hyperosmotic challenge of transitioning from freshwater (FW) to seawater (SW) during parr and smoltification (smolt) using the rainbow trout, Oncorhynchus mykiss. We examined the changes in the expression of AQPs mRNAs in the gills and intestine of the parr and smolt stages of rainbow trout transferred from FW to SW using quantitative real-time PCR in an osmotically changing environment [FW, SW, and recombinant AQP3 (rAQP3) injection at two dosage rates]. Correspondingly, AQPs were greater during smoltification than during parr stages in the rainbow trout. Plasma osmolality and gill $Na^+/K^+$-ATPase activity increased when the fish were exposed to SW, but these parameters decreased when the fish were exposed to SW following treatment with rAQP3 during the transition to seawater. Our results suggest that AQPs play an important role in water absorbing mechanisms associated with multiple AQP isoforms in a hyperosmotic environment.