• Journal of Animal Science and Technology
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• v.53 no.1
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• pp.67-74
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• 2011

The aim of this study was to understand the management level of pathogenic bacteria in HACCP system implemented animal farms. Microbial samples were collected from manure, floor, compost depot, manure on belt, low milk tank, dust in laying house and egg collector in HACCP system implemented Korean beef cattle, dairy cattle, swine, and laying Hens farms. O157, O111 and O26 strains of E. coli were not detected in HACCP system implemented Korean beef cattle farm. The detection rate of E. coli from manure and floor in HACCP system implemented cattle farms (Korean beef cattle and dairy farm) was lower than those of non-HACCP system implemented cattle farm. Salmonella spp. was detected in HACCP system implemented cattle farms (Korean beef cattle and dairy farm). Compared with pervious studies, lower detection rate of Salmonella spp. at floor and compost depot in HACCP system implemented swine and commercial layer farms were indicated. In conclusion, implementation of HACCP system in animal farms would enhance the management level of biological hazard compare to normal animal farms.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.313-320
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• 2009

This experiment was conducted to evaluate the effect of tillage system and fertilizer type on the forage yield and quality of Italian ryegrass. This experiment was a $2{\times}2$ factorial arrangement for two tillage systems (tillage and no-tillage) and two fertilizer type (chemical and manure compost). The plant height, dry matter (DM) content, DM yield, TDN (total digestible nutrients) yield, and CP (crude protein) yield have significant differences in tillage system of Italian ryegrass. Italian ryegrass cultivated with tillage (plow and rotary till) had lower plant height, DM yield, TDN yield and CP yield than no-tillage, while its DM content showed the opposite results. However, there were no significant differences in the results on fertilizer types. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were significantly higher in no-tillage compared to tillage. However, there was no significant difference in CP content on tillage system. TDN and relative feed value (RFV) were higher in tillage than no-tillage system (p<0.01). The TDN content was lower when using manure as fertilizer. No significant effects were observed for NDF and RFV in fertilizer type of Italian ryegrass. Based on the results of this study, tillage system and fertilizer type affected forage quality as well as forage yield. Forage yield of no-tillage was higher compared to tillage.

• Korean Journal of Organic Agriculture
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• v.7 no.1
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• pp.35-48
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• 1998

The total amount of dry matter for the green manure crops was great wheat> rye> barley> Italian ryegrass in that order. The green manure crope were verified to have the reducing effect of injury of successive croppingin peper, mainly reducing the occurance of the most important pepper disease, Phytophthora capsisi, and enhancing the pepper quality in the fruit length and diameter. The direct seeding using current commercial pepper variety was proved as not economical one. In the first year of compost application, the growth and yield of red pepper were rather somewhat decreasing compared with those of check plot applied with organic fertilizers. compst application increased the content of organic matter in soil, which suggested compost could be applied for the sustainable purposes. In preference analysis about taking the new technique, the smaller farmer's cultivation area the more they wanted to accept the LISA farming, compared with the conventional one, could be possible to save 12% in the inorganic fertilizer expenses but wasted 412% ant 163% in both organic fertilizer and operator labor expenses, respectively. At the same time, the LISA decreased 15% in production cost but increased 225% and 139% in organic fertilizer quantity and operator labor hours. Since there was a great deal of difference in technological and economic factors from two farming methods, LISA multi-goal decision modeling is further required.

• Journal of the Korean Society of Mineral and Energy Resources Engineers
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• v.55 no.6
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• pp.527-537
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• 2018

Column tests of a sulfate reducing bacteria (SRB) bioreactor were conducted to determine the design factors for sulfate-rich mine drainage. Various substrates were applied to the bioreactor, including cow manure and its mixture with a mushroom compost, with rice straw and limestone as subsidiary materials. This procedure provided a removal efficiency of up to 82% of the total sulfur with the mixture of cow manure (70%), mushroom compost (10%) and rice straw (20%), and higher efficiencies were observed after 2 days of retention time. In the downflow condition of the flow direction, oxygen supply and re-oxidation of the sulfates occurred, causing a decrease in sulfate removal efficiency. The addition of an inorganic sludge containing heavy metals, which was intended for production of metal-sulfides in the bioreactor, had a negative effect on the long-term operation owing to arsenic release and toxicity to the SRB. The results thus show that a bioreactor using a mixed substrate with cow manure and operating in the downflow direction could reduce sulfates and total dissolved sulfur content; this process confirms the applicability of the SRB bioreactor to sulfate-rich saline drainage.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1032-1036
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• 2012

A bulking agent is an essential material for composting of high-moisture livestock manure such as pig slurry. Sawdust has been used as the most popular bulking agent but resources are limited in Korea. In this study, the feasibility of rice hull as a bulking agent was examined for composting of solid pig manure. The solid pig manure was mixed with 15% sawdust (PM+SD15), 15% rice hull (PM+RH15), 10% sawdust and 5% rice hull (PM+SD10+RH5), and 5% sawdust and 10% rice hull (PM+SD5+RH10) based on fresh weight. These mixtures were composted for 35 days. The average temperature of the composting file for 35 days was higher in PM+SD10+RH5 and PM+SD5+RH10 than in PM+SD15 and PM+RH15. The mass loss of PM+SD10+RH5 and PM+SD5+RH10 were 36.7 and 36.4%, respectively, which were higher than that of PM+SD15 and PM+RH15. After composting, organic matter content and organic matter/nitrogen ratio in all treatments met the official standards of commercial fertilizers. We concluded that rice hull may be a good bulking agent for pig manure composting when it is used in mixture with sawdust.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.3
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• pp.88-92
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• 2001

This study was carried out to investigate about the effect of organic fertilizers(Pig manure fermentation compost; P, Organic fertilizer; B, F, Compost; D, and microorganism; M) on the growth and yield of the chinese cabbage and lactuca. At the standard applicated-amounts plot of organic fertilizer have appeared the best yield, but another the excess amount plot came into existence damage of fertilizer. However, according to the co-application of microorganism appeared certainly decreasing damage of fertilizers. Also at the same fertilizer and applicated-amounts plots were the higher than an-application of microorganism on the growth and yield. In conclusion, the excess application of organic fertilizer should be occurred fertilizer damages and increased on the growth and yield by use of microorganism.

• Journal of the Korean GEO-environmental Society
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• v.11 no.11
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• pp.27-36
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• 2010

This study was conducted to evaluate physicochemical parameters; temperature, pH, C/N ratio, water content, organic contents and volume in a pilot-scale(capacity : $100m^3$) ultra thermophilic aerobic composting. There were three types input: municipal wasted sludge, livestock manure and slurry, and food waste produced in Jung-Eb city. Each target material was carried out by the first fermentation(organic waste + seed culture) and the second one(organic waste + seed culture + recycle compost), respectively. During composting, only with supply of air and mixing, the temperature increased $90{\sim}105^{\circ}C$ after every mixing in both periods. The changes of pH, $O_2$, $CO_2$ and $NH_3$ represented typical organic decomposition pattern by microorganisms. Also, all other physicochemical parameters of ultra thermophilic aerobic composting process showed similar or better performance than these of general aerobic composting. Heavy metal concentration of fermented compost adapted to compost fertilizer regulation standard in the heavy metal and hazardous analysis.

• Journal of the Korea Organic Resources Recycling Association
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• v.28 no.4
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• pp.53-68
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• 2020

The concentrations of ammonia, hydrogen sulfide and fine dust were measured in the compost facility of a full-time Hanwoo breeding farms. The experiments were conducted in stack type composting facility(T1) and mechanical-stirred composting facilities(T2, T3). In the stack type composting facility, the highest temperature of compost pile was 46℃, and in the two mechanical-stirred composting facilities, it rose to 63℃ and 68℃, respectively. The concentrations of PM2.5 at T1, T2 were 15 ㎍/㎥ and 5~10 ㎍/㎥, respectively. And the concentration of PM2.5 at T3 was below 10 ㎍/㎥. The highest concentration of ammonia generated at T1 was 4 ppm, but no hydrogen sulfide was detected. The ammonia concentrations at T2 and T3 were 3 ppm and 4 ppm, respectively. However, hydrogen sulfide was not detected in both facilities. At T3, the ammonia concentration increased to 65 ppm at the point where the compost was stirred with a mechanical agitator. During composting period, the pH of the compost pile decreased from 9.06 to 8.94 and then increased to 9.14 as the composting period elapsed. The NaCl content of compost was 0.09% after composting process was complete. Moisture content of compost decreased from 65.9% to 62% as composting progressed. As composting proceeded, the content of volatile solids decreased from 65.6% to 64.7% and the content of TKN decreased from 1.327% to 1.095%.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1651-1658
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• 2013

Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollutants from wastewater under thermophilic conditions. We therefore isolated thermophilic facultative bacterial strains from wood chips that had been composted with swine manure under aerated high temperature conditions so as to identify strains with denitrifying ability. Nine different colonies were screened and 3 long rod-shaped bacterial strains designated as SG-01, SG-02, and SG-03 were selected. The strain SG-01 could be differentiated from SG-02 and SG-03 on the basis of the method that it used for sugar utilization. The 16S rRNA genes of this strain also had high sequence similarity with Geobacillus thermodenitrificans $465^T$ (99.6%). The optimal growth temperatures ($55^{\circ}C$), pH values (pH 7.0), and NaCl concentrations (1%) required for the growth of strain SG-01 were established. This strain reduced 1.18 mM nitrate and 1.45 mM nitrite in LB broth after 48 h of incubation. These results suggest that the G. thermodenitrificans SG-01 strain may be useful in the removal of nitrates and nitrites from wastewater generated as a result of livestock farming.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.888-895
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• 2015

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.