• Journal of Plant Biology
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• 제34권3호
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• pp.185-190
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• 1991

Hypocotyl-derived callus cultures of Brassica pekinensis cv. Chungseng were grown on Murashige-Skoog medium containing NaCl, Na2SO4 and mannitol to clarify the effect of salts on callus growth, proline accumulation and water relations. Na2SO4 was more inhibitory than NaCl when survival rate, growth and fresh:dry weight ratios of established callus were measured. Fresh weight in 0.25% NaCl treatment was more than twice as increasable in comparison to the same concentration of Na2SO4 or control. Proline concentration was increased on either salt, attained at highest levels at the 5th subculture, and fluctuated as affected by both salts or mannitol. Concentrations of redeucing sugars were sharply increased after 2 or 3 times of subculture and decreased by increasing subculture. The water and osmotic potential in callus reached a maximum negative value after two weeks in culture, regardless of salt type and mannitol and tugor remained relatively constant on both salt and mannitol treatments.

• 한국자기공명학회논문지
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• 제19권1호
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• pp.42-48
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• 2015

The mannitol transporter Enzyme $II^{Mtl}$ of the bacterial phosphotransferase system has two cytoplasmic phosphoryl transfer domains $IIA^{Mtl}$ and $IIB^{Mtl}$. The two domains are linked by a flexible peptide linker in mesophilic bacterial strains, whereas they are expressed as separated domains in thermophilic strains. Here, we carried out backbone assignment of $IIA^{Mtl}$ from thermophilic Thermoanaerobacter Tencongensis using a suite of heteronuclear triple resonance NMR spectroscopy. We have completed 94% of the backbone assignment, and obtained secondary structural information based on torsion angles derived from the chemical shifts. $IIA^{Mtl}$ of Thermoanaerobacter Tencongensis is predicted to have six ${\beta}$ strands and six ${\alpha}$ helices, which is analogous to $IIA^{Mtl}$ of Escherichia coli.

• 대한미생물학회지
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• 제20권1호
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• pp.13-23
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• 1985

This investigation was performed to isolate and identify Staphylococcus sp. from air at the 25 sites in one hospital in Daegu. Drug sensitivity tests bacteriologically were also studied. The results are summarized as follows: 1. In gram staining of isolated colonies, the number of staphylococci strains was 959(70.2%) among 1367 collected in the morning, and 653(66.2%) among 987 collected in the afternoon. 2. The number of isolates was slightly higher in samples collected from the outpatient treatment rooms and wards in the main building than in samples from the other sites. 3. In biochemical tests of 1612 strains of isolates gram positive cocci, the number of coagulase positive strains was 584(36.2%) and coagulase negative 1028(63.8%). In experiments on growth on mannitol agar containing 7.5% NaCl and mannitol fermentation tests, the number of mannitol fermentation strains was 423(30.8%) and nonfermenter was 951(59.2%). 4. In antibiotic sensitivity tests of 746 identified strains of staphylococci, coagulase positive strains showed resistance in. higher portions to penicillin(89.7%), chloramphenicol(64.6%), gentamycin (52.1%) and tetracycline(45.2%), whereas in lower portions to kanamycin(39.2%) and apmicillin(33.1%). 5. Among coagulase negative and mannitol fermenting strains, higher portions showed resistance to chloramphenicol(76.5%), penicillin(69.8%) and ampicillin(63.6%), whereas lower portions showed resistance to kanamycin(48.8%), tetracycline(39.5%) and gentamycin(34.0%). 6. The number of strains showing multiple resistance to above 6 antibiotics were 15(52(19.8%) among coagulase positive and mannitol non-fermenter and 36(22.2%) among coagulase negative and mannitol fermenter.

• 한국수산과학회지
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• 제2권1호
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• pp.71-82
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• 1969

The present investigations were carried out with the purpose of making clear the fundamental features of the seasonal variations in chemical composition of the brown algae. Three species of samples, Ecklonia cava, Sargassum sagamianum and Hizikia fusiforme have been analyzed monthly for their contents of total ash, crude protein, alginic acid, mannitol, and laminarin over a year period. Three kinds of samples were collected from the same locality, situated on the southern coast of the Che-ju Island, from September 1966 to August 1967. In addition, the comparative analysis was made on fronds and stipes of the plant for their chemical composition. The results obtained are summarized as follows: 1, In general, the three species examined underwent a similar mode of seasonal variation, and no essential difference was detected among them. 2. The chemical composition of the plant exhibited a considerable difference between the species. The content of total ash in H. fusiforme was remarkably higher than those in the two other species respectively. The alginic acid content was relatively high in S. sagamianum and low in H. fusiforme. The contents of crude protein, mannitol and laminarin were appreciably high in E. cava and low in H. fusiforme in general. 3. The most outstanding feature in the seasonal variation was that, in general, the total ash, crude protein, and alginic acid contents were at a maximum in the winter months while laminarin and mannitol contents were at a minimum. The converse was true in summer. Total ash-Maximum values were observed from December to February and minimum from August till October. Crude Protein-All species exhibited maxima in January and February, and minima from August to October. Alginic acid-Maximum contents occured from January to March and minimum from September to November. Mannitol-The maximum content of mannitol was In May and lune and minimum in January, February and March. Leminerin-Maximum content was in September and October, and minimum in January and February. 4. The wide seasonal variation in chemical constitution occured in the fronds, but the stipes showed a slight seasonal variation. In the chemical composition, the stipe was high in alginic acid, low in mannitol and laminarin. The reverse was in the frond.

• 생물환경조절학회지
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• 제20권2호
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• pp.144-149
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• 2011

본 연구는 품질증진제 처리가 산취(Chamaecereus silvestrii) '희망'의 모구품질과 표피 변화에 미치는 영향을 연구하였다. 생체중은 D-sorbitol 처리구보다 D-mannitol 처리구에서 감소율이 낮아 효과적이었다. Diniconazole 200ppm처리는 산취의 자구생육을 어느 정도(수치화) 억제하여 장기수송(50일)에 더 적합하였으며 자구색의 발현 어느 정도(수치화)에도 효과적이었다. 산취 모구의 표피구조는 비모란에 비해 하피 발달이 저조하고 어느 정도(수치화) 단층이며 세포벽도 얇아 어느 정도(수치화) 수송조건에 불리하였는데 생육의 억제효과가 높은 diniconazole 200ppm + D-mannitol 10,000ppm과 발색효과가 좋은 diniconazole 200ppm + wax 살포가 저장 중의 품질 유지에 적합하다고 판단된다.

• Biomolecules & Therapeutics
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• 제28권2호
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• pp.137-144
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• 2020

Epilepsy is a brain disorder that affects millions of people worldwide and is usually managed using currently available antiepileptic drugs, which result in adverse effects and are ineffective in approximately 20-25% of patients. Thus, there is growing interest in the development of new antiepileptic drugs with fewer side effects. In a previous study, we showed that a Rehmannia glutinosa (RG) water extract has protective effects against electroshock- and pentylenetetrazol (PTZ)-induced seizures, with fewer side effects. In this study, the objective was to identify the RG components that are responsible for its anticonvulsant effects. Initially, a number of RG components (aucubin, acteoside, catalpol, and mannitol) were screened, and the anticonvulsant effects of different doses of catalpol, mannitol, and their combination on electroshock- and chemically (PTZ or strychnine)-induced seizures in mice, were further assessed. Gamma-aminobutyric acid (GABA) receptor binding assay and electroencephalography (EEG) analysis were conducted to identify the potential underlying drug mechanism. Additionally, treated mice were tested using open-field and rotarod tests. Catalpol, mannitol, and their combination increased threshold against electroshock-induced seizures, and decreased the percentage of seizure responses induced by PTZ, a GABA antagonist. GABA receptor binding assay results revealed that catalpol and mannitol are associated with GABA receptor activity, and EEG analysis provided evidence that catalpol and mannitol have anticonvulsant effects against PTZ-induced seizures. In summary, our results indicate that catalpol and mannitol have anticonvulsant properties, and may mediate the protective effects of RG against seizures.

• 대한미생물학회지
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• 제17권1호
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• pp.67-74
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• 1982

비병원근무자(非病院勤務者)인 부산대학교(釜山大學校) 자연과학대학(自然科學大學) 생물학계열(生物學系列) 18명(名), 미생물학과(微生物學科) 2학년(學年) 31명(名), 미생물학과(微生物學科) 3학년(學年) 37명(名)과 병원근무자(病院勤務者)인 간호과학생(看護科學生) 30명(名), 간호원(看護員) 30명(名), 수술근무간호원(手術勤務看護員) 30명(名), 의사(醫師) 30명(名), 총(總) 256명(名)을 대상(對象)으로 하여 비강(鼻腔)에서 황색포도구균(黃色葡萄球菌)을 분리(分離)하여 얻은 성적(成績)은 다음과 같다. 1. 비병원근무자(非病院勤務者) 대학생(大學生)에서 황색포도구균분리율(黃色葡萄球菌分離率)은 49예(例)(29.7%), 병원근무자(病院勤務者)에서는 67예(例)(55.8%)이었다. 2. 비병원근무자(非病院勤務者) 대학생(大學生)의 비강(鼻腔)에서 분리(分離)한 황색포도구균(黃色葡萄球菌)의 40예(例)에서 coagulase 양성균주(陽性菌株) 29예(例)(72.5%), 음성균주(陰性菌株) 11예(例)(27.5%)이고, mannitol 분해(分解) 양성균주(陽性菌株) 29예(例)(72.5%), 음성균주(陰性菌株) 11예(例)(27.5%)이었다. 3. 병원근무자(病院勤務者)의 비강(鼻腔)에서 분리(分離)한 황색포도구균(黃色葡萄球菌)의 67예(例)에서 coagulase 양성균주(陽性菌株) 59예(例)(58.1%), 음성균주(陰性菌株) 8예(例)(11.9%)이고, mannitol 분해양성균주(分解陽性菌株) 49예(例)(73.1%), 음성균주(陰性菌株) 18예(例)(26.9%)이었다. 4. 분리균주중(分離菌株中)에서 coagulase양성(陽性) 및 mannitol분해양성(分解陽性) 49균주(菌株)에 대(對)한 용혈반응(溶血反應)의 감수성(感受性)의 순위(順位)는 가토(家兎) 40예(例)(81.6%), 모르못트 25예(例)(53.5%), 면양(緬羊) 13예(例)(26.5%)이고, 닭 및 사람의 적혈구(赤血球)에는 용혈반응음성(溶血反應陰性)이었다. 5. 분리균주중(分離菌株中)에서 coagulase양성(陽性) 및 mannitol분해양성(分解陽性) 49균주(菌株)에 대(對)한 용혈반응(溶血反應)은 전주(全株)에 대(對)해서 각각(各各) 음성(陰性)이었다. 6. 화학요법제(化學療法劑)에 대(對)한 분리균주(分離菌株)에 대(對)한 성적(成績)은 비병원근무자(非病院勤務者) 분리균주(分離菌株)는 대부분(大部分) 감수성(感愛性)이 있으나 병원근무자(病院勤務者) 분리균주(分離菌株)는 대부분(大部分) 저항성(抵抗性)을 나타냄을 관찰(觀察)할 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제25권1호
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• pp.143-146
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• 2004

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권4호
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• pp.234-236
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• 2002

A process for the production of mannitol from fructose (5% to 25%) using Leuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out In batch or fed-batch fermentations without aeration at 28$\^{C}$, pH 5.0. When 5% fructose was used In batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the frurtose concentration was Increased to 10%, the yield dropped to 59.6% of the theoretical value. However, In the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fruttose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply the final yield was 83.7% of the theoretical yield. When 20% fructose was used In the same manner, the yield was 89.5% of theoretical yield.

• The Plant Pathology Journal
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• 제27권4호
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• pp.390-395
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• 2011

Fusarium wilt caused by Fusarium oxysporum has become a serious problem world-wide and relies heavily on chemical fungicides. We selected Pseudomonas sp. NJ134 to develop an effective biocontrol strategy. This strain shows strong antagonistic activity against F. oxysporum. Biochemical analyses of ethyl-acetate extracts of NJ134 culture filtrates showed that 2,4-diacetylphloroglucinol (DAPG) was the major compound inhibiting in vitro growth of F. oxysporum. DAPG production was greatly enhanced in the NJ134 strain by adding mannitol to the growth media, and in vitro antagonistic activity against F. oxysporum increased. Bioformulations developed from growth of NJ134 in sterile bean media with mannitol as the carbon source under plastic bags resulted in effective biocontrol efficacy against Fusarium wilt. The efficacy of the bioformulated product depended on the carbon source and dose. Mannitol amendment in the bean-based formulation showed strong effective biocontrol against tomato Fusarium wilt through increased DAPG levels and a higher cell density compared to that in a glucose-amended formulation. These results suggest that this bioformulated product could be a new effective biocontrol system to control Fusarium wilt in the field.