• 한국연초학회지
• /
• 제4권1호
• /
• pp.79-81
• /
• 1982

Cytoplasmic male- sterile Hyangchio, derived from male- sterile Burley 21 containing N. megalosiphon cycloplasm, and male- fertile Hyangchio (Korean local aromatic variety) were evaluated in replicated pot trials in green-house. Male- sterile Hyangchio was comparable to the male- fertile Hyangchio for six agronomic traits and total alkaloids content. The only significant difference was that the male- sterile Hyangchio flowered 2.3 days later than the male- fertile Hyangchio. M ale- sterile Hyangchio has increased the yield than the male- fertile Hyangchio significantly in case of non- topped.

• 한국작물학회지
• /
• 제31권1호
• /
• pp.74-77
• /
• 1986

단순열성 유전자에 의한 GMS민 IR36ms에 가야벼, 남풍벼, 신광벼 및 수원 296 등 4개 한국 수도품종을 각각 4회 연속 Bulk로 교잡시킨 BC$_3$F$_2$에서 웅성불임 개체를 얻어서 여기에 각 반복친을 1회 더 Backcross시킨 BC$_4$F$_2$세대에서 웅성불임 개체들을 선별하였다. 여기에 각 반복친을 교잡시켜 4품종의 Msms 유전자형을 얻고 이를 다시 BC$_4$F$_2$에서 선발한 웅성불임 개체(msms)에 교잡시키므로서 가야벼ms, 남풍벼ms, 신광벼ms 및 수원 296ms를 육성하였다. 이들 4개 웅성불임 계통들의 주당수수, 수당영화수, 출수기 및 수장은 모품종과 차이가 있었으나 웅성불임 계통들의 간장은 모품종에 비하여 단축되었다. 동일한 웅성불임 유전자를 가진 GMS 계통도 반복친에 따라 자연교잡 정도의 현저한 차이가 있었다. 가야벼ms는 11.5%, 남풍벼 ms는 13.1%, 신광벼ms는 1.9% 그리고 수원 296ms는 12.7%의 자연교잡율을 나타내었다. 화분친으로부터 15~90cm 거리 내에서는 가야벼 mS 및 남풍벼ms의 자연교잡율의 거리에 따른 차가 인정되지 않았다.

• 한국육종학회지
• /
• 제42권3호
• /
• pp.298-301
• /
• 2010

'원예30002'는 웅성불임계통으로 초형은 반개장형, 구색은 황갈색이며 도복기는 5월 6일로 비교적 도복기가 빠른 조생 계통이다. 초장은 43 cm, 엽수는 7매이며 엽초경과 엽초장은 각각 15.5 cm, 18 cm이다. 구중은 283 g으로 대구형이며 구형지수는 93으로 고구형 계통이다. 저장성은 중간정도이나 내한성이 강한 특성이 있다. 채종 특성으로 채종 모구의 개화기는 5월 24일이며 화경수는 3개로 비교적 적고 화구폭은 90 mm이며 100% 웅성불임이다.

• 한국식물병리학회지
• /
• 제14권3호
• /
• pp.212-216
• /
• 1998

Capsicum pepper selections fro PI163192, PI241670, PI244670, PI271322, PI308787, PI322719, and PI369994 were confirmed to be non-hypersensitively resistant to race 3 of Xanthomonas campestris pv. vesicatoria. A resistant cultivar,‘SR’, was shown to be hypersensitive. Four Koran local cultivars, a cytoplasmic male sterile line (A-line) and its maintainer (B-line) were highly susceptible. The resistant selections and cultivars were crossed with a male sterile A-line (Smsms) and fertility of their F1 hybrids was examined by observing he pollen production, testing pollen germination, and quantifying the amount of pollen produced per anther to identify the genotype interacting with the male sterile cytoplasm. The seven resistant PI selections turned out to be restorers (N(S)MsMs) and‘SR’to be a maintainer (Nmsms).

• 한국작물학회지
• /
• 제30권4호
• /
• pp.427-430
• /
• 1985

수도의 1대잡종 종자생산을 위한 기초적인 정보를 얻고자 한국품종 배경의 세포질적 유전적 웅성불임계통 수원296A, 수원304A 및 수원310A와 유전적 웅성불임계통 수원296GMS 등과 대조품종인 중공의 세포질적 유전적 웅성불임계통 Zhenshan97A를 공시하여 주두노출정도, 시간별 개영수 및 개영각도 변화 등을 조사하고 자연교잡률과 관련시켜 검토한 바 다음과 같은 결과를 얻었다. 1. 한국품종을 배경으로 한 웅성불임계통들의 주두노출 정도가 중공의 CGMS Zhenshan97A 보다 불량하였고 특히 Japonica형 CGMS인 수원304A의 주두노출이 불량하였다. 2. 시간별 개영수의 Peak의 조만과 유무는 자연교잡률과 관련이 적었다. 3. 개영각이 크고 장시간 개영이 지속되는 웅성불임계통의 자연교잡이 높았다. 개영각이 작으면서 장시간 개영되어도 효과가 적었다. 4. 동일품종을 배경으로 했을 경우 GMS가 CGMS보다 자연교잡에 유리했다. 5. 웅성불임계통은 정상계통에 비해 개영 지속시간이 길었다.

• Journal of Plant Biotechnology
• /
• 제44권4호
• /
• pp.364-371
• /
• 2017

The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.

• 원예과학기술지
• /
• 제29권1호
• /
• pp.53-60
• /
• 2011

Cytoplasmic male sterility (CMS) induced by mutant mitochondria genome, has been used for commercial seed production of $F_1$ hybrid cultivars in diverse crops. In pepper (Capsicum annuum L.), two sterile cytoplasm specific gene organization, atp6-2 and coxII were identified. An open reading frame, orf456 nearby coxII gene has been speculated to induce male sterility (MS) by mutagenic analysis. Moreover, molecular markers for atp6-2 and coxII of mitochondrial genotype (mitotype) were developed. However, the Cytoplasmic MS specific markers, atp6SCAR and coxIISCAR markers appeared in both N and S cytoplasms when polymerase chain reaction (PCR) cycles prolonged more than 40 cycles. Since the reported molecular markers were dominant markers, the presence of the faint sterile-specific band in normal cytoplasm may lead to the mis-classification of pepper breeding lines. To solve this problem, one common forward primer and two different reverse primers specific to normal coxII and sterile orf456 genes were designed after analyzing their gene organizations. By using these three primers, N and S coxII specific bands were co-amplified in male-sterile lines, but only normal coxII specific band was amplified in maintainer lines. Since the reverse primer for sterile coxII was specifically designed 275 bp downstream of orf456, relatively stable PCR amplification patterns were observed regardless of the number of PCR cycles. These primer sets easily identified different mitotypes among the divergent breeding lines, commercial cultivars and diverse germplasms.

• 한국육종학회지
• /
• 제42권3호
• /
• pp.294-297
• /
• 2010

양파 중간모본 웅성불임 계통 '원예30001'은 2000년 Ginque 자식 집단에서 웅성불임개체를 선발하여 고정종 자식계통 YG-1-1을 교배하여 2002년에 유지계통을 선발하고 2009년까지 4회 여교잡을 하여 계통의 안정성을 확인한 후 MMS17로 명명하여 2009년에 직무육성신품종 심사를 거쳐 2010년에 국립종자원에 '원예30001'로 명명하여 품종보호 출원하였다. '원예30001'은 웅성불임(CMS-S)계통으로 초형은 반개장형이며 도복기는 5월 20일이다. 초장은 57 cm, 엽수 8매, 엽초경은 15.9 cm로 지상부의 생육이 왕성하며 구중은 260 g, 구형지수 89인 원형 황색 양파계통이다. 노균병에 중간 정도의 저항성이 있으나 내한성과 저장성은 강하다. 모구의 채종 특성으로 개화기는 5월 23일이며 구당 화경수는 6개, 화경 장은 135 cm이며 화구 폭은 84 mm이며 100% 웅성불임 이다.

• 방사선산업학회지
• /
• 제4권3호
• /
• pp.239-246
• /
• 2010

The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ($^{60}Co$) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from $M_1$ generation at the doses (10 to 50 Gy). We also found that some $M_1$ plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using $KI-I_2$ solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutations like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses.

• 원예과학기술지
• /
• 제30권1호
• /
• pp.73-78
• /
• 2012

KC00256, KC00406, KC00462, KC00463, KC00820, and KC00821, the genetic resources that have previously been reported as moderately resistant to Phytophthora capsici, as well as the line KC01322, a new source of moderate resistance introduced from Laos, were tested against two strains (Pc003 and Pc005) of P. capsici. We also determined the nuclear restorer genotypes of these lines, in regards to their interaction with cytoplasmic male sterility, through crossing the resources with cytoplasmic male sterile Punggok-A (Srfrf) and determining the fertility of the $F_1$ hybrids. The studied lines exhibited a low level of resistance to both the strains of P. capsici compared to highly resistant CM334, but their response was fairly consistent for both P. capsici strains. KC00406, KC00462, KC00463, and KC01322 produced stable, male fertile $F_1$ plants indicating that they are restorers with genotype N(S)RfRf. KC00821 produced male sterile $F_1$ plants and was identified as a maintainer with genotype Nrfrf. The $F_1$ plants of the KC00820 cross, however, set a few male fertile flowers in the greenhouse at seedling stage, then became male sterile after being transplanted to the plastic greenhouse soil in May and remained so to the end of the growing season. Therefore, KC00820 is an unstable maintainer with genotype Nrfrf. The moderate resistance exhibited by these genetic resources may be integrated into breeding programs aimed at promoting higher levels resistance via recurrent selection or hybridization.