Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene

  • Kumar, Pravin (University School of Biotechnology, Guru Gobind Singh Indraprastha University) ;
  • Kaur, Kulwinder (Department of Plant Molecular Biology, University of Delhi South Campus) ;
  • Purty, Ram Singh (University School of Biotechnology, Guru Gobind Singh Indraprastha University) ;
  • Mohan, Madan (Department of Plant Molecular Biology, University of Delhi South Campus) ;
  • Burma, Pradeep Kumar (Department of Genetics, University of Delhi South Campus)
  • Received : 2017.08.31
  • Accepted : 2017.11.29
  • Published : 2017.12.31
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Abstract

The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.

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References

  1. Bae HK, Kang HG, Kim GJ, Eu HJ, Oh SA, Song JT, Chung IK, Eun MY, Park SK (2010) Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility. Plant Breeding 129(6): 647-651 https://doi.org/10.1111/j.1439-0523.2010.01784.x
  2. Bisht NC, Jagannath A, Gupta V, Burma PK, Pental D (2004) A two gene - two promoter system for enhanced expression of a restorer gene (barstar) and development of improved fertility restorer lines for hybrid seed production in crop plants. Molecular Breeding 14(2):129-144 https://doi.org/10.1023/B:MOLB.0000038002.45312.08
  3. Budar F, Pelletier G (2001) Male sterility in plants: Occurrence, determinism, significance and use. Comptes Rendus de l'Academie Des Sciences - Serie III 324(6):543-550 https://doi.org/10.1016/S0764-4469(01)01324-5
  4. Cao B, Huang Z, Chen G, Lei J (2010) Restoring pollen fertility in transgenic male-sterile eggplant by Cre/loxp-mediated site-specific recombination system. Genetics and Molecular Biology 33(2):298-307 https://doi.org/10.1590/S1415-47572010005000043
  5. Dellaporta S L, Wood J, Hicks JB (1983) A Plant DNA Mini preparation : Version II. Plant Mol. Biol. Report 1:19-21 https://doi.org/10.1007/BF02712670
  6. Deveshwar P, Bovill WD, Sharma R, Able JA, Kapoor S (2011) Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice. BMC Plant Biology 11(1):78 https://doi.org/10.1186/1471-2229-11-78
  7. Ding Z, Wu X, Wang T (2002) The rice tapetum-specific gene RA39 encodes a type I ribosome-inactivating protein. Sexual Plant Reproduction 15(4):205-212 https://doi.org/10.1007/s00497-002-0156-2
  8. Harvey MJ (2004) The use of cytoplasmic male sterility for hybrid seed production. In: Molecular Biology and Biotechnology of Plant Organelles: Chloroplasts and Mitochondria (Daniel H and Chase C):623-634
  9. Heslop-Harrison J, Heslop-Harrison Y (1970) Evaluation of pollen viability by enzymatically induced fluorescence, intracellular hydrolysis of fluorescein diacetate. Stain Technol 145:115-120
  10. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal: For Cell and Molecular Biology 6(2):271-282 https://doi.org/10.1046/j.1365-313X.1994.6020271.x
  11. Huang J-Z, E Z-G, Zhang H-L, Shu Q-Y (2014) Workable male sterility systems for hybrid rice: Genetics, biochemistry, molecular biology, and utilization. Rice 7(1):13 https://doi.org/10.1186/s12284-014-0013-6
  12. Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Burma PK, Pental D (2001) The use of a Spacer DNA fragment insulates the tissue-specific expression of a cytotoxic gene (barnase) and allows high-frequency generation of transgenic male sterile lines in Brassica juncea L. Molecular Breeding 8(1):11-23 https://doi.org/10.1023/A:1011916216191
  13. Jeon JS, Chung YY, Lee S, Yi G H, Oh BG, An G (1999) Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza sativa L.). Plant Molecular Biology 39(1):35-44 https://doi.org/10.1023/A:1006157603096
  14. Kempe K, Gils M (2011) Pollination control technologies for hybrid breeding. Molecular Breeding 27(4):417-437 https://doi.org/10.1007/s11032-011-9555-0
  15. Khurana R, Kapoor S, Tyagi A K (2012) Anthology of Anther/Pollen-Specific Promoters and Transcription Factors. Critical Reviews in Plant Sciences 31(5):359-390 https://doi.org/10.1080/07352689.2012.664986
  16. Khurana R, Kathuria H, Mukhopadhyay A, Kapoor S, Tyagi AK (2013) A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis. Biotechnology Letters 35(3):455-462 https://doi.org/10.1007/s10529-012-1100-7
  17. Kropff MJ, Cassman KG, Peng S, Setter TL, Mathews RB (1994) Quantitive understanding of rice yield potential. In: K.G. Cassman (Editor), Breaking the Yield barrier. International Rice Research Institute, Los Banos, Philippines: 21-38
  18. Kuriakose B, Arun V, Gnanamanickam SS, Thomas G (2009) Tissue-specific expression in transgenic rice and Arabidopsis thaliana plants of GUS gene driven by the 5′ regulatory sequences of an anther specific rice gene YY2. Plant Science 177(5):390-397 https://doi.org/10.1016/j.plantsci.2009.06.016
  19. Liu J, Yu Y, Lei J, Chen G, Cao B (2009) Study on Agrobacterium-Mediated Transformation of Pepper with Barnase and Cre Gene. Agricultural Sciences in China 8(8):947-955 https://doi.org/10.1016/S1671-2927(08)60299-0
  20. Luo H, Lee JY, Hu Q, Nelson-Vasilchik K, Eitas TK, Lickwar C, Kausch AP, Chandlee JM, Hodges T K (2006) RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Molecular Biology 62(3):397-408 https://doi.org/10.1007/s11103-006-9031-0
  21. Mariani C, Beuckeleer M D, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347(6295):737-741 https://doi.org/10.1038/347737a0
  22. Mariani C, Gossele V, De Beuckeleer M, De Block M, Goldberg R. B, De Greef W, Leemans J (1992) A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357:384-387 https://doi.org/10.1038/357384a0
  23. Park J-I, Hakozaki H, Endo M, Takada Y, Ito H, Uchida M, Okabe T, Watanabe M (2006). Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.). Plant Cell Reports 25(5): 466-74 https://doi.org/10.1007/s00299-005-0077-2
  24. Rai M, Datta K, Parkhi V, Tan J, Oliva N, Chawla HS, Datta SK (2007) Variable T-DNA linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice. Plant Cell Reports 26(8):1221-31 https://doi.org/10.1007/s00299-007-0333-8
  25. Ray K, Bisht NC, Pental D, Burma PK (2007) Development of barnase/barstar transgenic for hybrid seed production in Indian oilseed mustard (Brassica juncea L. Czern and Coss) using a mutant acetolactate synthase gene conferring resistance to imidazolinone-based herbicide 'Persuit'. Current Science 93:1390-1396
  26. Rawat P, Singh AK, Ray K, Chaudhary B, Kumar S, Gautam T, Kanoria S, Kaur G, Kumar P, Pental D, Burma PK (2011) Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics. Journal of Biosciences 36(2): 363-376 https://doi.org/10.1007/s12038-011-9074-5
  27. Rosellini D, Pezzotti M, Veronesi F (2001) Characterization of transgenic male sterility in alfalfa. Euphytica 118(3):313-319 Singh SK, Bhati PK, Sharma A, Sahu V (2015) Super hybrid rice in China and India: Current status and future prospects. Int.J.Agric. Biol. 17:221-232
  28. Swapna L, Khurana R, Vijaya Kumar S, Tyagi AK, Rao KV (2011) Pollen-specific expression of oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and arabidopsis transgenic systems. Molecular Biotechnology 48(1):49-59 https://doi.org/10.1007/s12033-010-9347-5
  29. Tsuchiya T, Toriyama K, Ejiri SI, Hinata K (1994) Molecular characterization of rice genes specifically expressed in the anther tapetum. Plant Molecular Biology 26(6):1737-1746 https://doi.org/10.1007/BF00019488
  30. Wu HM, Cheun AY (2000) Programmed cell death in plant reproduction. Plant Molecular Biology 44(3):267-81 https://doi.org/10.1023/A:1026536324081
  31. Yokoi S, Tsuchiya T, Toriyama, K., Hinata K (1997) Tapetum-specific expression of theOsg6B promoter-$\beta$-glucuronidase gene in transgenic rice. Plant Cell Reports 16:363-367
  32. Yoshida S, Forno DA, Cock JH, Gomez KA (1976) Routine procedure for growing rice plants in culture solution. In: Laboratory manual for physiological studies of rice, International Rice Research Institute, Manila, The Philippines: 61-66
  33. Yuan LP, Fu XQ (1995) Technology of hybrid rice production. Rome: FAO:84
  34. Zhang D, Liang W, Yin C, Zong J, Gu F, Zhang D (2010) OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiology 154(1):149-162 https://doi.org/10.1104/pp.110.158865
  35. Zhang J, Cai L, Cheng J, Mao H, Fan X, Meng Z, Chan KM, Zhang H, Qi J, Ji L, Hong Y (2008) Transgene integration and organization in cotton (Gossypium hirsutum L.) genome. Transgenic Research 17(2):293-306 https://doi.org/10.1007/s11248-007-9101-3