• Applied Microscopy
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• 제42권2호
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• pp.67-76
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• 2012

고강성 나노섬유 생산이 가능한 전기방사장치 방사노즐의 설계를 위해 자연을 모방하는 공학적 방법론을 도입하여, 무당거미(Nephila clavata L.Koch) 대병상선의 토사관과 그 생체방적 시스템이 지닌 미세구조적 특성을 고해상도의 전자현미경 관찰을 통해 분석하였다. 자연계에서 가장 강도가 높은 드래그라인을 생성, 분비하는 대병상선의 토사관은 bullet type spigot을 통해 전방적돌기 표면에 개구되어 있었으며, 신축성 구조를 지닌 토사관 말단마디의 노즐을 통해 고체상의 실크가 생성되었다. 분비낭과 토사관 사이를 연결한 분비관은 루프를 형성한 후 방적돌기에 수납되었고, 분비관 내강의 직경은 점진적으로 축소되어 노즐을 통해 방사되는 실크의 직경은 펀넬 부위의 1/10 이하인 것으로 관찰되었다. 한편, 실크 중합과정에서 수분을 제거하기 위한 큐티클의 특수구조가 관찰되었는데, 분비낭의 펀넬 부위에서는 해면상 큐티클 구조가 그리고 분비관의 말단부에서는 비후된 subcuticle 구조와 함께 잘 발달된 상피의 미세융모 층이 확인되었다. 또한 분비관의 내강부 큐티클 표면에서 확인된 나선형 강선구조는 실크 전구물질의 신속한 유동을 촉진하는 장치일 것으로 해석되었다.

• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.857-864
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• 2002

The aim of this study is to investigate the end of filaments of the different toothbrushes in the market through the stereomicroscope and to evaluate the % of rounded-end filaments considered to be acceptable. 9 brands, total 11 type toothbrushes were tested. 2 toothbrushes of each type which is marked as rounded-end filaments were tested. The toothbrushes which are not marked as rounded-end filaments were excluded. The domestic as well as foreign toothbrushes which are familiar to consumers were tested. 2 tufts of each toothbrushes were cut and examined by stereomicroscope using $40{\times}$ magnification. The procedure was carried out with blind-technique, and the digital photographs were taken. Besides the % of rounded-end filaments, total tufts number, material of the tuft, stiffness, and other special characteristics were recorded. By the classification of Silverstone and Featherstone, rounded-end filaments were examined and counted. The results shows that there are different range of rounded-end filaments according to the toothbrush types(17.7%-91.2%). Atman toothbrush has the most rounded-end filaments(91.2%) among the observed toothbrushes, and the Advantage Plus(Ora1-B) has the next(86.75%). E-Clean #411 has the least(17.70%) and E-Clean #410 of the same brand has also low % rounded-end filaments(20.60%). While G.U.M #409(Butler) has 67.90% rounded-end filaments, G.U.M #471 of the Same brand has comparative low 41.83% rounded-end filaments. 4 types of total 11 have the rounded-end filaments over 80%, however other 4 types have under even 50%. Considering that the correct brushing habit with a toothbrush which has rounded-end filaments can protect the gingival injury and tooth abrasion, it is thought that we dentists need to give the correct information about toothbrush to the patients

• Journal of Periodontal and Implant Science
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• 제31권4호
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• pp.811-822
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• 2001

최근 임플란트를 원하고, 필요로 하는 환자가 증가하면서 술전의 정확한 진단이 보다 중요해졌고 다양한 진단방법이 소개되고 이용되어져 왔다. 그 중 보편적으로 가장 널리 사용되어지는 방법이 방사선 촬영법이고 그 중에서도 파노라마방사선사진이다. 하지만 파노라마방사선사진의 용이함과 간편성에도 불구하고 그 방법 자체가 가지고 있는 한계점 때문에 CT/MPR(Computer Tomography/Multiplanar Reformatting)이 일부 환자에서는 반드시 필요하다는 주장이 대다수이다. 본 연구의 목적은 하악 구치부에 임플란트 시술을 받은 환자를 대상으로 파노라마방사선사진의 확대율을 알아보고 파노라마방사선사진과 CT/MPR에서 치조정-하악관간 거리가 실제 어느 정도 차이를 보이는지, 그리고 CT/MPR에 대한 파노라마방사선사진의 확대율에 환자의 하악골의 경사도와 하악관의 해부학적 위치가 어떤 영향을 미치는지를 알아보는 것이다. 본 연구는 서울대학 치과병원 치주과를 내원하여 하악구치부에 Dental Implant 시술을 받은 15명의 환자, 32개 부위를 대상으로, 임플란트 시술을 위한 술전 파노라마방사선사진과 CT/MPR, 술후의 파노라마방사선사진, CT/MPR상을 자료로, 임플란트가 식립될 위치에서 치조정에서 하악관의 길이, 하악골의 협설폭경, 하악골의 설측면에서 하악관의 수평길이, 임플란트 매식체의 설측에서 하악관까지의 수평거리를 계측하였고 이 해부학적 계측치들이 CT/MPR에 대한 파노라마방사선사진의 확대율과 어떤 상관관계에 있는지 Pearson's correlation coefficient test를 이용해 살펴보았다. 1. CT/MPR에 대한 파노라마방사선사진의 확대율은 CT/MPR상에서 하악골 단면의 협설폭경(Pearson test에서의 상관관계 분석시, 상관계수 r=-0.604), 임플란트 매식체의 설측에서 하악관까지의 수평거리(r=-0.515), 하악골의 설측면에서 하악관까지의 수평거리(r=-0.640)와 뚜렷한 음적 선형관계가 있었다. 2. CT/MPR에 대한 파노라마방사선사진의 확대율은 CT/MPR상에서 하악골의 장축의 각도와는 뚜렷한 양적 선형관계(Pearson test에서의 상관관계 분석시, 상관계수 r=-0.446)가 있었다. 3. 여러 요소중 CT/MPR에 대한 파노라마방사선사진의 확대율과 가장 유의성있는 연관성을 보이는 것은 CT/MPR상에서 하악골의 설측면에서 하악관까지의 수평거리였다(r=-0.640) 4. CT/MPR에 대한 파노라마방사선사진의 확대율은 일반적으로 생각되어지는 파노라마방사선사진의 확대율 125%보다 큰, 약 135%로 나왔다. 이상의 결과로 볼 때, 하악골의 설측면에서 하악관까지의 수평거리가 CT/MPR에 대한 파노라마방사선사진의 확대율에 가장 영향을 미치는 요소로 추정되며 확대율은 125%보다 높은 것으로 나타났다.

• Journal of Periodontal and Implant Science
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• 제31권4호
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• pp.749-763
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• 2001

Osseointegrated titanium implants have become an integral therapy for the replacement of teeth lost. For dental implant materials, titanium, hydroxyapatite and alumina oxide have been used, which of them, titanium implants are in wide use today. Titanium is known for its high corrosion resistance and biocompatability, because of the high stability of oxide layer mainly consists of $TiO_2$. With the development of peri-implantitis, the implant surface is changed in surface topography and element composition. None of the treatments for cleaning and detoxification of implant surface is efficient to remove surface contamination from contaminated titanium implants to such extent that the original surface elemental composition. In this sights, the purpose of this study was to evaluate rough surface titanium implants by means of scanning electron microscopy(SEM) and X-ray photoelectron spectroscopy(XPS) with respect to surface appearance and surface elemental composition. Moreover, it was also the aim to get the base for treatments of peri-implantitis. For the SEM and XPS study, rough surface titanium models were fabricated for control group. Six experimental groups were evaluated: 1) long-time room exposure, 2 ) air-powder abrasive cleaning for 1min, 3) burnishing in citric acid(pH1) for 1min, 4) burnishing in citric acid for 3min, 5) burnishing in tetracycline for 1min, 6) burnishing in tetracycline for 3min. All experimental treatments were followed by 1min of rinsing with distilled water. The results were as follows: 1. SEM observations of all experimental groups showed that any changes in surface topography were not detected when compared with control group. (750 X magnification) 2. XPS analysis showed that in all experimental groups, titanium and oxygen were increased and carbon was decreased, when compared with control group. 3. XPS analysis showed that the level of titanium, oxygen and carbon in the experimental group 3(citric acid treatment for 1min, followed by 1min of distilled water irrigation) reached to the level of control group. 4. XPS analysis showed that significant differences were not detected between the experimental group 1 and the other experimental groups except of experimental group 3. The Ti. level of experimental group 2, airpowder abrasive treatment for lmin followed by 1min of saline irrigation, was lower than the Ti. level of tetracycline treated groups, experimental group 5 and 6. From the result of this study, it may be concluded that the 1min of citric acid treatment followed by same time of rinsing with distilled water gave the best results from elemental points of view, and can be used safely to treat peri-implantitis.

• 대한치과보철학회지
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• 제43권5호
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• pp.611-621
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• 2005

State of problem: Zirconia all-ceramic restoration fabricated with CAM system is on an increasing trend in dentistry. However, evaluation of the marginal and internal fits of zirconia bridge seldomly have been reported. Purpose: This study was to evaluate the at of margin and internal surface in posterior 3-unit zirconia bridge framework fabricated with CAM system(DeguDent, Germany). Material and Method: Preparations of secondary premolar and secondary molar on artificial resin model were performed for fabrication of 3-unit posterior bridge framework. Fits of 5 zirconia bridge framework were compared with 5 precious ceramo-metal alloy framework(V-GnathosPlus, Metalor, Switzerland), and prepared margins were designed to chamfer and shoulder finishing line. Each framework was cemented to epoxy resin model with reinforced glass ionomer(FujiCEM, GC Co., Japan), embedded in acrylic resin and sectioned in two planes, mesio-distal and buccolingual. Samples were divided into six pieces by sectioning and had two pieces of each surface(i.e mesial, distal, buccal and lingual surface) per abutment, so there were eight measuring points in each abutment. External gap was measured at the margin and internal gaps were measured at the margin, axial and occlusal surface. Gaps were observed under the measuring microscope(Compact measuring microscope STM5; Olympus, Japan) at a magnification of $\times100$. T-test were used to determine the statistic significance of the different gaps between zirconia and metal framework. Results and Conclusion: 1. External and internal marginal gaps of zirconia and metal framework were in clinically acceptable range. External marginal gaps were not different significantly between zirconia$(81.9{\mu}m)$ and metal $(81.3{\mu}m)$ framework and internal marginal gaps of zirconia $(44.6{\mu}m)$ were smaller than those of metal framework $(58.6{\mu}m)$. 2. Internal axial gaps of zirconia framework$(96.7{\mu}m)$ were larger than those of metal frame-work$(78.1{\mu}m)$ significantly and adversely, internal occlusal gaps of zirconia frame-work$(89.4{\mu}m)$ were smaller than those of metal framework $(104.9{\mu}m)$ significantly. 3. There were no significant differences in external and internal marginal gaps between chamfer and shoulder finish line when zirconia frameworks were fabricated.

• Journal of Magnetics
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• 제19권2호
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• pp.185-191
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• 2014

In this study we evaluated that flow rate changes affect the (time of flight) TOF image and contrast-enhanced (CE) in a three-dimensional TOF angiography. We used a 3.0T MR System, a nonpulsatile flow rate model. Saline was used as a fluid injected at a flow rate of 11.4 cm/sec by auto injector. The fluid signal strength, phantom body signal strength and background signal strength were measured at 1, 5, 10, 15, 20 and 25-th cross-section in the experienced images and then they were used to determine signal-to-noise ratio and contrast-to-noise ratio. The inlet, middle and outlet length were measured using coronal images obtained through the maximum intensity projection method. As a result, the length of inner cavity was 2.66 mm with no difference among the inlet, middle and outlet length. We also could know that the magnification rate is 49-55.6% in inlet part, 49-59% in middle part and 49-59% in outlet part, and so the image is generally larger than in the actual measurement. Signal-to-noise ratio and contrast-to-noise ratio were negatively correlated with the fluid velocity and so we could see that signal-to-noise ratio and contrast-to-noise ratio are reduced by faster fluid velocity. Signal-to-noise ratio was 42.2-52.5 in 5-25th section and contrast-to-noise ratio was from 34.0-46.1 also not different, but there was a difference in the 1st section. The smallest 3D TOF MRA measure was $2.51{\pm}0.12mm$ with a flow velocity of 40 cm/s. Consequently, 3D TOF MRA tests show that the faster fluid velocity decreases the signal-to-noise ratio and contrast-to-noise ratio, and basically it can be determined that 3D TOF MRA and 3D CE MRA are displayed larger than in the actual measurement.

• Restorative Dentistry and Endodontics
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• 제24권3호
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• pp.453-464
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• 1999

The non-vital bleaching technique has been used widely as a very effective treatment method on discolored non-vital teeth. But periodontal tissue deterioration and cervical external root resorption have been reported because of the high toxicity of hydrogen peroxide in bleaching agents. So in previous studies, placement of base over the root canal obturation prior to bleaching has been suggested in order to prevent microleakage of bleaching agents, however, the effectiveness of base is still controversial. The purpose of this study was to evaluate the effects of base and root canal sealer on prevention of leakage of bleaching agents in non-vital bleaching. Fifty-two extracted sound teeth with single root were used. For root canal obturation, Tubuli seal$^{(R)}$(Kerr Co., USA) was used in 39 teeth and in others, AH-26$^{(R)}$(De Trey Dentsply, Inc., Switzerland) was used as a root canal sealer. 26 teeth among the teeth obturated with Tubuli seal$^{(R)}$ were divided into two groups, and Dentin cement$^{(R)}$(GC corp., Japan) and JRM$^{(R)}$(De Trey Dentsply, Inc. Germany) were used in each group as a intracanal base. In all teeth, non-vital bleaching using bleaching agent mixed with methylene blue dye was performed and all specimens were stored in $37^{\circ}C$ water bath for 72 hours. After sectioning longitudinally, the depth of dye leakage was measured with digital vernier calipers under the stereobinocular microscope using ${\times}40$ magnification. It can be concluded as follows: 1. The microleakage of bleaching agent was observed ill all groups regardless of type of the base and the sealer. 2. The microleakage in the groups using AH-26$^{(R)}$ as a sealer was significantly reduced (p<0.05). 3. In the groups with intracanal base, micro leakage was observed through almost the whole depth of the base and there was no significant difference between Dentin cement$^{(R)}$ and IRM$^{(R)}$ group(p>0.05). In conclusion, all the basing materials and the sealers in this study did not prevent the microleakage of bleaching agent. Therefore further studies and attempts to seal off the pulp chamber will be necessary.

• 한국안광학회지
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• 제13권2호
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• pp.17-22
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• 2008

목적: X선용 545 nm 형광판을 위한 결상광학계 개발. 방법: 캐논(Canon)사 CX2-70 모델을 참조하여 설계 기준을 정한 다음 Sigma 2000 광학 설계 프로그램으로 X선용 545 nm 형광판을 위한 결상광학계를 설계 제작하였다. 결과: 새로 설계 제작되어진 X선용 545 nm 형광판 결상광학계의 배율은 -0.225배이고, 이미지 크기는 90 mm${\times}$90 mm이며, 분해선폭은 30% MTF 값 기준으로 0.033 mm인 특성을 보여준다. 이는 캐논 CX2-70 모델보다 큰 화면으로 판독을 할 수 있고, 판독의 분해능도 우수함을 의미한다. 또한 이미지 측 NA값이 -0.196으로 캐논 CX2-70 모 델 -0.139보다 약$\sqrt{2}$배 크게 하였고, 물체 측 NA값도 0.044로 캐논 CX2-70 모델 0.022보다 약 2배 크게 하였다. 이는 필름의 감도를 4배 증가 시킬 수 있는 것을 의미하며 X선의 피폭시간을 1/4로 줄일 수 있음을 뜻한다. 결론: 이미지 크기가 90 mm${\times}$90 mm이고, 15 lines/mm에서 MTF 30% 이상인 특성을 가지며, 캐논 CX2-70 모델에 비해 X선 피폭시간을 1/4로 줄일 수 있는 새로운 X선용 545 nm 형광판 결상광학계를 설계 제작하였다.

• Restorative Dentistry and Endodontics
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• 제26권4호
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• pp.307-315
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• 2001

The application of Nd:YAG laser and irrigants to the root surface can change its surface configurations. The purpose of this study was to investigate the effects of Nd:YAG laser and irrigants on the apical seal of obturated canals. In this study, 66 single rooted teeth were randomly assigned to 4 group of 14 teeth each. 8 teeth were served us positive and negative controls. The teeth were divided into 6 groups as follows. Group A: Nd:YAG laser, 5% NaOCl + Rc-prep Group B: Nd:YAG laser, Saline Group C: 5% NaOCl + Rc-prep Group D: Saline Group E: Positive control Group F: Negative control 66 teeth were instrumented using Maillefer ProFile$^{\circledR}$ (Orifice Shapers, .04 taper, .06 taper Dentsply, Switzerland). Two of each group were selected at random, and the canal wall surfaces were examined under a SEM. 12 teeth of each group were obturated using by lateral condensation technique. Specimens were immersed in india ink for 7days, decalcified by 10% nitric acid, dehydrated by 75. 80. 85, 90, 95 and 100% alcohol in order cleared by methyl salicylate and then measured of dye penetration with stereomicroscope($\times$15 magnification) and Image Pro plus. The data were analyzed statistically by one-way ANOVA test and Duncan's Multiple Range test. The results were as follows : 1. The mean leakage was 0.128$\pm$0.376 for group A, 0.237$\pm$0.325 for group B, 0.397$\pm$0.468 for group C, 0.586$\pm$0.402 for group D, and there were statistically significant differences between group A and group D, group B and group D. (p<0.05). 2. Group A had better sealing ability than Group C, but there was statistically no significant differences. (p>0.05). 3. Group B had better sealing ability than Group D and there was statistically significant difference. (p<0.05). 4 Group A had better sealing ability than Group B, but there was statistically no significant difference. (p>0.05). 5. Group C had better sealing ability than Group D, but there was statistically no significant difference. (p>0.05). 6. As a result of observation under SEM, Smear layers were removed in Group A, B. but Smear layers were partially removed and smear plugs were remained in Group C, Smear layers were not removed in Group D. To be specially, Melting of smear layer were showed in Group C. 7. These results suggests that the laser has a potential in reducing the apical microleakage of obturated canals.

• Restorative Dentistry and Endodontics
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• 제27권3호
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• pp.249-256
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• 2002

The use of flowable composite resins as liners in class II packable composite restoration has been suggested by some manufacturers. However, the contributions of this technique are unproven. The purpose of this study was to compare the gingival microleakage in class II packable composite restorations with or without the use of flowable composite resins as liners. Slot cavities were prepared on both proximals of 80 extracted human molars and randomly assigned to 8 groups of 20 each. The gingival margins were located at 1mm above CEJ in 80 cavities (group1-4) and 1mm below CEJ in 80 cavities (group5-8). The prepared teeth were mounted in the customized tray with adjacent teeth to simulate clinical conditions and metallic matrix band (Sectional matrix) and wooden wedges were applied. After acid etching and application of Single Bond, each group was restored with the following materials using incremental placement technique: Group 1,5 (Filtek P60), group 2, 3, 4 and group 6, 7, 8 (AeliteFlo, TetricFlow, Revolution/ Filtek P60). All specimens were thermocycled 500 times between 5$^{\circ}C$ and 55$^{\circ}C$ with 1 mimute dwell time, immersed 2% methylene blue dye for 24 hours and then rinsed with tab water. The specimens were embedded in clear resin and sectioned longitudinally through the center of restoration with a low speed diamond saw. Dye penetration at gingival margin was viewed at 20 magnification and analyzed on a scale of 0 to 4. Kruscal-Wallis One way analysis and Mann-Whitney Rank sum test were used to analyze the results. The results of this study were as follows. 1. The leakage values seen at the enamel margin were significantly lower than those seen at the dentin margin(P<0.05). 2. On the enamel margin, packable composite resins with flowable liners showed lower leakage than those without flowable liners, but there were no significant differences among the four groups(P>0.05). 3. On the dentin margin, four groups demonstrated moderate to severe leakage, and there were no significant differences in leakage values(P>0.05).