• Title/Summary/Keyword: magnetic separation

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Utilization of functionalized magnetic nanoparticles for high throughput DNA separation

  • Jang, Jeong-Ho
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.11a
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    • pp.12.2-12.2
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    • 2009
  • The work describes anoptimized process to highly efficient and convenient preparation in highthroughput magnetic human DNA separation with chemically functionalizedsilica-coated magnetic nanoparticles. The effect of nanoparticle's size and the surface's hydrophilicity change were studied for magnetic DNA separation process, inwhich the optimum efficiency was explored via the function of the amino-groupnumbers, particle size, the amount of the nanoparticles used, and theconcentration of NaCl salt. The DNA adsorption yields were high in terms of theamount of triamino-functionalized nanoparticles used, and the average particlesize was 25 nm. The adsorption efficiency of aminofunctionalized nanoparticleswas the 4-5 times (80-100%) higher compared to silica-coated nanoparticles only(10-20%). DNA desorption efficiency showed an optimum level of over 0.7 M ofthe NaCl concentration. To elucidate the agglomeration of nanoparticles afterelectrostatic interaction, the Guinier plots were calculated from small angleX-ray diffractions in a comparison of the results of electron diffraction TEM,and confocal laser scanning microscopy. Additionally, the direct separation ofhuman genomic DNA was achieved from human saliva and whole blood with highefficiency.

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Mineralogy of Sea Sand Near Ongjingun through the Separation Processes (옹진군 해사의 선별공정에 따른 광물학적 특성)

  • Chae, Soo-Chun;Shin, Hee-Young;Bae, In-Kook;Kwon, Sung-Won;Lee, Chun-Oh;Kim, Jung-Yoon;Jang, Young-Nam
    • Journal of the Mineralogical Society of Korea
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    • v.24 no.1
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    • pp.1-17
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    • 2011
  • Mineralogical study was carried out for heavy minerals in the sea sand near Ongjingun bay, Kyonggi-do separated using the gravity and magnetic separators. Ilmenite, zircon and minor monazite and garnet were valuable minerals with gangue minerals of quartz, K-feldspar, plagioclase, muscovite, hornblende, epidote and chlorite. Quantitative analysis with SIROQUANT program showed that the contents of ilmenite separated with the gravity separation (the shaking table separation), the 1st step magnetic separation (rare earth magnetic separation) and the 2nd step magnetic separation (the Eddy current magnetic separation) were increased into 0.8, 18.3, and 48.7%, respectively. The content of ilmenite, monazite and zircon were recalculated based on the chemical composition of the representative and heavy fraction products of raw sand, the 1 step and 2 step gravity separations, and the 1 step and 2 step magnetic separations. The content increased to 0.23, 0.55, 5.22, 16.17, and 44.99% in ilmenite, 0.11, 0.02, 0.16, 0.51, and 1.19% in monazite. Although the zircon content did not differ over the processes (0.13, 0.12, 0.11, 0.15, and 0.10%), the improved recovery of zircon is expected by applying sieving process because of its high content (27%) in the fine grain size fraction (< 140#) of the 2 step gravity separation.

Treatment of rolling cooling waste water by superconductor HGMS method (초전도 자기분리에 의한 냉연공정 폐수처리)

  • Kim, Tae-Hyung;Ha, Dong-Woo;Oh, Sang-Soo;Kim, Young-Hun;Ha, Tae-Wook
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.295-295
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    • 2008
  • This study introduced waste water treatment method applied superconductor HGMS(High Gradient Magnetic Separation). HGMS method treat high efficient method for various waste water. we have surveyed superconducting magnetic separation technology and reviewed the status of related industries using applied superconductivity. We fabricated the prototypes of magnetic matrix filter consisting of stainless steel mesh, which is a core component in the magnetic separation system. In our basic preliminary experiment using HGMS, it was made clear that the fine para-magnetic particles in the rolling colling wasted water obtained from rolling process of POSCO can be separated with high efficiency.

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Separation Between Soil Particles and Magnetic Beads by Magnetic Force (자력을 이용한 토양입자와 마이크로자성체의 분리 연구)

  • So, Hyung-Suk;Shin, Hyun-Chul;Yoo, Yeong-Seok
    • Journal of the Korean Magnetics Society
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    • v.15 no.2
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    • pp.76-80
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    • 2005
  • It was evaluated whether magnetic beads able to add the functionality of environment purification can be employed in processing soil pollutants. In this study, the micro scale magnetic beads containing carboxyl groups were mixed with water and the soil $(<0.025{\cal}mm) filtered through a sieve, and then it was agitated before isolating the magnetic substances by the use of outer magnetic force. The factors considered at this step were the ratio of soil to magnetic beads, ratio of soil to water, size of the tube where the reaction occur, and intensity of the magnetic force. From the separation experiment between soil and magnetic beads, it was concluded that the magnetic beads and water quantity have an impact on the degree of separation, yet the size of the tube and magnetic force does not have a considerable effect upon that in this small-scaled experiment. Through this experiment, the reaction conditions were optimized to achieve $90\~100\%$ of separation. Therefore, it was concluded that when the functionalized magnetic beads is introduced to environmental processing, it is able to be adopted to the soil processing as well as the water processing.

A Study on the Removal Efficiency of Heavy Metals in Daenam Mine Agricultural Soil Using Heavy metal Properties by Physical separation (대남광산 농경지 토양 내 중금속 특성에 따른 물리적 선별 처리효율에 관한 연구)

  • ParK, Chan Oh;Hong, Dong-Ho;Lee, Jai-Young;Lee, Young Jae;Lee, Jin-Soo
    • Journal of Soil and Groundwater Environment
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    • v.18 no.5
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    • pp.46-55
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    • 2013
  • The main objective was to evaluate the efficiencies of different separation techniques, such as gravity separation, magnetic separation, and aerial separation. Zinc and cadmium removal efficiencies by gravity separation and magnetic separation were 28.3~29.3% and 19.1%, respectively, and were higher than the efficiency obtained by aerial separation. Results showed that the combination of gravity separation and magnetic separation in series which was to maximize the removal efficiencies gave removal efficiency of 21.5~38.7% for zinc and 22.1~23.4% for cadmium. The mass of soil meeting the regulation standards for zinc and cadmium after retrieval from the combined separation process accounted for approximately 80% of the treated soil that would be reusable without the pre-treatment procedure as the neutralization process using in the soil washing method. Physical separation techniques utilizing heavy metal properties are the alternative method to remediate heavy-metal contaminated soils in environmental and economic aspects.

Buffer-Optimized High Gradient Magnetic Separation: Target Cell Capture Efficiency is Predicted by Linear Bead-Capture Theory

  • Waseem, Shahid;Udomsangpetch, Rachanee;Bhakdi, Sebastian C.
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.125-132
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    • 2016
  • High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a bead-capture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency ($E_t$) in a highly significant manner.

Study on separation of nonferrous metal utilizing magneto-Archimedes method

  • Ito, Yusuke;Akiyama, Yoko
    • Progress in Superconductivity and Cryogenics
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    • v.21 no.2
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    • pp.10-14
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    • 2019
  • In order to improve resource value, separation of nonferrous metals obtained from crushed materials of home appliances is required. In this study, we aimed to develop a continuous separation system by magneto-Archimedes method using magnetic fluid as a medium and the permanent magnet as a magnetic field source. Firstly, the separation conditions were examined in which only copper is settled and the difference in levitation positions between aluminum and other metals are over 1 cm. Based on the results, levitation experiment of each metal and separation experiment from the mixture of nonferrous metals were confirmed. The separation experiment showed that the continuous separation of copper and aluminum from a mixture of nonferrous metals is possible.

Separation of Magnetic/non-Magnetic Particles by an Electromagnetic Fluidized Bed (전자석 유동층에 의한 자성/비자성 입자의 분리)

  • 김용하;서인국
    • Resources Recycling
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    • v.6 no.1
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    • pp.17-22
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    • 1997
  • An electromagnetic fluidized bed was proposed for the continuous separation of magnetic particles from the fine a admixtures with nonHmagnetic particles. The effects of operating variables on the magnetic fraction in the separated p particles were examined, including superficial gas velocity, mixing fraction of magnetic particles (= 100-mixing fraction of n non-magnetic particles) in the admixture, and electric current supplied to the electwmagnet. It was found that the s separation was possible when a magnetic force formed by the electromagnets works on the magnetic particles over the hydrodynamic force caused by a gas stream for fluidizing the fine admixture.

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Synthesis and Phosphorus Adsorption Characteristics of Zirconium Magnetic Adsorbent Having Magnetic Separation Capability (자기분리가 가능한 지르코늄 자성 흡착제의 합성과 인 흡착 특성)

  • Lim, Dae-Seok;Kim, Yeon-Hyung;Kim, Dong-Rak;Lee, Tae-Gu;Lim, Hak-Sang
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.3
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    • pp.431-442
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    • 2012
  • The purpose of this study, is to separate magnetic separation devices using permanent magnets by using magnetization characteristics remaining in treated water after adsorption and synthesizing phosphorus adsorbent capable of magnetic separation for efficient removal of phosphorus. The synthesis of the adsorbent which set Zirconium(Zr) having high friendly features for phosphorus as an element, and by synthesizing Iron Oxide($Fe_3O_4$, another name of $Fe_3O_4$ is magnetite) being able to grant magnetism to Zirconium Sulfate($Zr(SO_4)_2$), zirconium magnetic adsorbent(ZM) were manufactured. In order to consider the phosphorus adsorption characteristics of adsorbent ZM, batch adsorption experiment was performed, and based on the results, pH effect, adsorption isotherm, adsorption kinetics, and magnetic separation have been explore. As the experiment result, adsorbent ZM showed a tendency that the adsorption number was decreased rapidly at pH 13; however, it was showed a high amount of phosphorus removal in other range and it showed the highest amount of phosphorus removal in pH 6 of neutral range. In addtion, the Langmuir adsorption isotherm model is matched well, and D-R adsorption isotherm model is ranged 14.43kJ/mol indicating ion exchange mechanism. The result shown adsorption kinetics match well to the Pseudo-second-order kinetic model. The adsorbent ZM's capablility of regenerating NaOH and $H_2SO_4$, was high selectivity on the phosphorus without impacts on the other anions. The results of applying the treated water after adsorption of phosphorus to the magnetic separation device by using permanent magnets, shows that capture of the adsorbent by the magnetization filter was perfect. And they show the possibility of utilization on the phosphorus removal in water.