• 제목/요약/키워드: magnetic separation

검색결과 407건 처리시간 0.027초

Utilization of functionalized magnetic nanoparticles for high throughput DNA separation

  • 장정호
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2009년도 추계학술발표대회
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    • pp.12.2-12.2
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    • 2009
  • The work describes anoptimized process to highly efficient and convenient preparation in highthroughput magnetic human DNA separation with chemically functionalizedsilica-coated magnetic nanoparticles. The effect of nanoparticle's size and the surface's hydrophilicity change were studied for magnetic DNA separation process, inwhich the optimum efficiency was explored via the function of the amino-groupnumbers, particle size, the amount of the nanoparticles used, and theconcentration of NaCl salt. The DNA adsorption yields were high in terms of theamount of triamino-functionalized nanoparticles used, and the average particlesize was 25 nm. The adsorption efficiency of aminofunctionalized nanoparticleswas the 4-5 times (80-100%) higher compared to silica-coated nanoparticles only(10-20%). DNA desorption efficiency showed an optimum level of over 0.7 M ofthe NaCl concentration. To elucidate the agglomeration of nanoparticles afterelectrostatic interaction, the Guinier plots were calculated from small angleX-ray diffractions in a comparison of the results of electron diffraction TEM,and confocal laser scanning microscopy. Additionally, the direct separation ofhuman genomic DNA was achieved from human saliva and whole blood with highefficiency.

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옹진군 해사의 선별공정에 따른 광물학적 특성 (Mineralogy of Sea Sand Near Ongjingun through the Separation Processes)

  • 채수천;신희영;배인국;권성원;이춘오;김정윤;장영남
    • 한국광물학회지
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    • 제24권1호
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    • pp.1-17
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    • 2011
  • 경기도 옹진군부근의 근해로부터 채취된 해사로부터 비중 및 자력선별을 통한 중광물의 회수공정을 실시하여, 광물학적 특성을 분석하였다. 옹진군 해사 내에 포함된 중광물로는 티탄철석, 저어콘, 소량의 모나자이트 및 석류석 등이 있으며, 맥석광물로는 석영, K-장석, 사장석, 백운모, 보통각섬석, 녹염석, 녹니석 등이 있다. SIROQUANT 프로그램을 이용한 광물 정량분석 결과, 요동테이블 선별, 영구자석을 이용한 자력선별(rare-earth magnetic separation) 및 전자석 자력선별(Eddy current magnetic separation)로부터 회수한 유용광물인 티탄철석의 함량은 각각 0.8, 18.3, 48.7%로 증가되었다. 또한 원사, 1차 비중, 2차 비중, 1차 자력 및 2차 자력선별 산물 중 대표시료 및 중광물군에 대한 화학분석치로부터 재계산시, 티탄철석 및 모나자이트의 함량은 각각 0.23, 0.55, 5.22, 16.17, 44.99% 및 0.11, 0.02%, 0.16, 0.51, 1.19%로 증가하였다. 그러나 저어콘의 경우에는 0.13, 0.12, 0.11, 0.15, 0.10%로 큰 변화를 보이지 않았지만, 2차 비중선별시료 중 가장 미립인 -140 메시 입단의 경우 0.27%의 높은 값을 보임으로써 입도분리를 통한 선별 시, 회수율 증대가 기대된다.

초전도 자기분리에 의한 냉연공정 폐수처리 (Treatment of rolling cooling waste water by superconductor HGMS method)

  • 김태형;하동우;오상수;김영훈;하태욱
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2008년도 하계학술대회 논문집 Vol.9
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    • pp.295-295
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    • 2008
  • This study introduced waste water treatment method applied superconductor HGMS(High Gradient Magnetic Separation). HGMS method treat high efficient method for various waste water. we have surveyed superconducting magnetic separation technology and reviewed the status of related industries using applied superconductivity. We fabricated the prototypes of magnetic matrix filter consisting of stainless steel mesh, which is a core component in the magnetic separation system. In our basic preliminary experiment using HGMS, it was made clear that the fine para-magnetic particles in the rolling colling wasted water obtained from rolling process of POSCO can be separated with high efficiency.

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자력을 이용한 토양입자와 마이크로자성체의 분리 연구 (Separation Between Soil Particles and Magnetic Beads by Magnetic Force)

  • 서형석;신현철;유영석
    • 한국자기학회지
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    • 제15권2호
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    • pp.76-80
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    • 2005
  • 환경정화 등의 기능성을 부여할 수 있는 고분자자성체가 토양 오염물처리에 사용될 수 있는지 여부를 살펴보았다. 본 연구에서는 수산화기를 지닌 마이크로 고분자자성체를 분체로 걸러진 토양$(<0.025{\cal}mm)$, 물과 함께 혼합하고 교반시킨 후 1.2 Tesla의 자력을 지닌 자석으로 자성체를 분리하였다. 이때 고려되어진 인자는 토양과 고분자자성체의 비율, 토양과 물의 비율, 반응용기의 크기 그리고 자력의 크기였다. 토양과 고분자자성체의 분리실험 결과, 고분자자성체와 물의 양이 분리도에 영향을 미친 반면 반응용기의 크기와 자력은 이 소규모의 실험에서 별다른 영향이 없었다. 본 실험을 통하여 전체적으로 반응조건을 최적화시켜 $90{\~}100{\%}$의 분리도를 달성할 수 있었다. 이로서 기능성고분자를 환경 처리에 적용함에 있어서, 수처리 뿐만 아니라 토양처리에도 사용이 가능함을 확인하였다.

대남광산 농경지 토양 내 중금속 특성에 따른 물리적 선별 처리효율에 관한 연구 (A Study on the Removal Efficiency of Heavy Metals in Daenam Mine Agricultural Soil Using Heavy metal Properties by Physical separation)

  • 박찬오;홍동호;이재영;이영재;이진수
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제18권5호
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    • pp.46-55
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    • 2013
  • The main objective was to evaluate the efficiencies of different separation techniques, such as gravity separation, magnetic separation, and aerial separation. Zinc and cadmium removal efficiencies by gravity separation and magnetic separation were 28.3~29.3% and 19.1%, respectively, and were higher than the efficiency obtained by aerial separation. Results showed that the combination of gravity separation and magnetic separation in series which was to maximize the removal efficiencies gave removal efficiency of 21.5~38.7% for zinc and 22.1~23.4% for cadmium. The mass of soil meeting the regulation standards for zinc and cadmium after retrieval from the combined separation process accounted for approximately 80% of the treated soil that would be reusable without the pre-treatment procedure as the neutralization process using in the soil washing method. Physical separation techniques utilizing heavy metal properties are the alternative method to remediate heavy-metal contaminated soils in environmental and economic aspects.

Buffer-Optimized High Gradient Magnetic Separation: Target Cell Capture Efficiency is Predicted by Linear Bead-Capture Theory

  • Waseem, Shahid;Udomsangpetch, Rachanee;Bhakdi, Sebastian C.
    • Journal of Magnetics
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    • 제21권1호
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    • pp.125-132
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    • 2016
  • High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a bead-capture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency ($E_t$) in a highly significant manner.

Study on separation of nonferrous metal utilizing magneto-Archimedes method

  • Ito, Yusuke;Akiyama, Yoko
    • 한국초전도ㆍ저온공학회논문지
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    • 제21권2호
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    • pp.10-14
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    • 2019
  • In order to improve resource value, separation of nonferrous metals obtained from crushed materials of home appliances is required. In this study, we aimed to develop a continuous separation system by magneto-Archimedes method using magnetic fluid as a medium and the permanent magnet as a magnetic field source. Firstly, the separation conditions were examined in which only copper is settled and the difference in levitation positions between aluminum and other metals are over 1 cm. Based on the results, levitation experiment of each metal and separation experiment from the mixture of nonferrous metals were confirmed. The separation experiment showed that the continuous separation of copper and aluminum from a mixture of nonferrous metals is possible.

전자석 유동층에 의한 자성/비자성 입자의 분리 (Separation of Magnetic/non-Magnetic Particles by an Electromagnetic Fluidized Bed)

  • 김용하;서인국
    • 자원리싸이클링
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    • 제6권1호
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    • pp.17-22
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    • 1997
  • 미립의 자성/비자성 입자의 혼합분체로부터 자성 입자만을 연속적으로 분리할 수 있는 전자석 유동층이 제안되었고 본 선별기에 의해 분리된 입자분중 자성 분율에 대한 공탑 가스유속, 혼합분체내 자성 입자의 분율(=100-비자성 입자의 분율) 및 전자석으로 공급되는 전류 세기와 같은 조업변수의 영향이 규명되었다. 전자석 유동층에 의한 자성 분리는 미립의 혼합분체내 자성입자에 작용하는 전자서의 자력이 분체를 유동화시키는 가스흐름으로 인한 유체력보다 클 때 가능함이 밝혀졌다.

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자기분리가 가능한 지르코늄 자성 흡착제의 합성과 인 흡착 특성 (Synthesis and Phosphorus Adsorption Characteristics of Zirconium Magnetic Adsorbent Having Magnetic Separation Capability)

  • 임대석;김은형;김동락;이태구;임학상
    • 상하수도학회지
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    • 제26권3호
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    • pp.431-442
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    • 2012
  • The purpose of this study, is to separate magnetic separation devices using permanent magnets by using magnetization characteristics remaining in treated water after adsorption and synthesizing phosphorus adsorbent capable of magnetic separation for efficient removal of phosphorus. The synthesis of the adsorbent which set Zirconium(Zr) having high friendly features for phosphorus as an element, and by synthesizing Iron Oxide($Fe_3O_4$, another name of $Fe_3O_4$ is magnetite) being able to grant magnetism to Zirconium Sulfate($Zr(SO_4)_2$), zirconium magnetic adsorbent(ZM) were manufactured. In order to consider the phosphorus adsorption characteristics of adsorbent ZM, batch adsorption experiment was performed, and based on the results, pH effect, adsorption isotherm, adsorption kinetics, and magnetic separation have been explore. As the experiment result, adsorbent ZM showed a tendency that the adsorption number was decreased rapidly at pH 13; however, it was showed a high amount of phosphorus removal in other range and it showed the highest amount of phosphorus removal in pH 6 of neutral range. In addtion, the Langmuir adsorption isotherm model is matched well, and D-R adsorption isotherm model is ranged 14.43kJ/mol indicating ion exchange mechanism. The result shown adsorption kinetics match well to the Pseudo-second-order kinetic model. The adsorbent ZM's capablility of regenerating NaOH and $H_2SO_4$, was high selectivity on the phosphorus without impacts on the other anions. The results of applying the treated water after adsorption of phosphorus to the magnetic separation device by using permanent magnets, shows that capture of the adsorbent by the magnetization filter was perfect. And they show the possibility of utilization on the phosphorus removal in water.