• 대한족부족관절학회지
• /
• 제18권3호
• /
• pp.100-107
• /
• 2014

Purpose: The purpose of this study is to evaluate the efficacy of mesenchymal stem cell (MSC) isolation by the magnetic-activated cell sorting (MACS) method in tendon tissue-derived cells compared to the colony picking method for isolation of MSCs by picking colony-forming cells. Materials and Methods: Human tendon-derived cells were isolated by enzyme digestion using normal tendon tissues from three donors. We used the magnetic kit and well-known MSC markers (CD90 or CD105) to isolate MSCs in tendon-derived cells using MACS. Cloning cylinders were used to isolate colony-forming cells having MSC characteristics in tendon-derived cells. Colony-forming unit-fibroblast (CFU-F) assay was used to evaluate the self-renewal capacity of cells isolated using the colony picking method or MACS. For comparison of differentiation potentials into osteogenic or adipogenic lineage between two groups, alizarin red S and oil red O staining were performed at 14 days after induction of differentiation in vitro. Results: Flow cytometry results showed that early passage tendon-derived cells expressed CD44 in 99.13%, CD90 in 56.51%, and CD105 in 86.19%. In the CFU-F assay, CD90+ or CD105+ cells isolated with MACS showed larger colony formation in size than cells isolated using the colony picking method. We also observed that CD90+ or CD105+ cells were constantly differentiated into both osteogenic and adipogenic lineages in cells from all donors, whereas cells isolated using the colony picking method were heterogeneous in differentiation potentials to the osteogenic and adipogenic lineages. Conclusion: CD90+ or CD105+ cells isolated using MACS showed superior MSC characteristics in the self-renewal and multi-differentiation capacities compared with cells isolated using the colony picking method.

• 한국결정성장학회지
• /
• 제10권1호
• /
• pp.17-22
• /
• 2000

수직보자력이 크고 CoCrPt에 비하여 noise 저감 효과가 큰 것으로 알려진 CoCrPtTa계 합금박막의 수직이방성을 더욱 개선하기 위해 Ti를 우선 증착하였다. 본 연구에서는 Ti 두께 변화에 따른 비교적 높은 수직보자력치를 보였던 $CoCr_{16.2}Pt_{10.8}Ta_4$Ti/glass 시편에서 자기적 특성과 자구형상의 변화를 살펴보았다. 또한 VSM(Vibrating Sample Magnetometer)을 이용하여 M-H loop를 측정하였으며 자구형상은 MFM(Magnetic Force Microscopy)로 관찰하였다. 육방정 Ti가 하지층으로서 우선증착되면 bare glass 직접 증착되는 경우보다 CoCrPtTa 자성층의 수직이방성에 현저한 향상을 가져왔으며 Ti의 두께가 두꺼울수록 c-축 배향성도 개선되었다. MFM 결과에 의하면 Ti두레가 20 nm에서 90 nm로 증가함에 따라 자구형상이 연속적인 stripe type에서 mass type으로 분절된 형태로 변하였다. 이는 Ti의 증착이 비자성 Cr 편석 거동에 영향을 미쳐 자화반전시 인접 columnar grain의 자화벡터들간의 상호교환작용을 억제하는 자기적 분리 효과에 기여했음을 의미한다. 아울러 이와 같은 거동은 Ti가 CoCr계 자성층의 수직이방성을 개선하는데 있어서 형상자기이방성적인 측면에서의 기여가 현저함을 의미하기도 한다.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2011년도 임시총회 및 추계학술발표회
• /
• pp.20-20
• /
• 2011

In this study, we analyzed 80%MeOH extract of fruits of sorbaria sorbifolia var. stellipila MAX. to measure the total antioxidant capacity by oxygen radical absorbance capacity (ORAC) assay, individual flavonoid content by high-performance liquid chromatography (HPLC). n-Hexane ($1.02{\pm}0.036$), $CH_2Cl_2$ ($0.95{\pm}0.025$), EtOAc ($1.94{\pm}0.065$), n-BuOH ($1.98{\pm}0.054$), D.W. ($1.2{\pm}0.032$) fractions were examined antioxidative activity by ORAC assay. It was revealed that EtOAc($1.94{\pm}0.065$), n-BuOH($1.98{\pm}0.054$) fractions had significant antioxidative activity. The isolation and separation were facilitated using open column chromatography, while separation, purification and identification were accomplished by using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR).

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2000년도 영호남학술대회 논문집
• /
• pp.29-33
• /
• 2000

This paper describes on the temperature characteristics of a SDB(silicon-wafer direct bonding) SOI(silicon-on-insulator) Hall sensor. Using the buried oxide $SiO_2$ as a dielectrical isolation layer, a SDB SOI Hall sensor without pn junction isolation has been fabricated on the Si/$SiO_2$/Si structure. The Hall voltage and the sensitivity of the implemented SOI Hall sensor show good linearity with respect to the applied magnetic flux density and supplied current. In the temperature range of 25 to $300^{\circ}C$, the shifts of TCO(temperature coefficient of the offset voltage) and TCS(temperature coefficient of the product sensitivity) are less than ${\pm}6.7{\times}10^{-3}/^{\circ}C$ and ${\pm}8.2{\times}10^{-4}/^{\circ}C$, respectively. These results indicate that the SDB SOI structure has potential for the development of a silicon Hall sensor with a high-sensitivity and high-temperature operation.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2000년도 하계학술대회 논문집
• /
• pp.565-568
• /
• 2000

This paper describes on the temperature characteristics of a SDB(silicon-wafer direct bonding) SOI(silicon-on-insulator) Hall sensor. Using the buried oxide $SiO_2$ as a dielectrical isolation layer, a SDB SOI Hall sensor without pn junction isolation has been fabricated on the Si/$SiO_2$/Si structure. The Hall voltage and the sensitivity of the implemented SOI Hall sensor show good linearity with respect to the applied magnetic flux density and supplied current. In the temperature range of 25 to $300^{\circ}C$, the shifts of TCO(temperature coefficient of the offset voltage) and TCS(temperature coefficient of the product sensitivity) are less than $\pm 6.7$$\times$$10^{-3}$/$^{\circ}C$ and $\pm 8.2$$\times$$10^{-4}$/$^{\circ}C$respectively. These results indicate that the SDB SOI structure has potential for the development of a silicon Hall sensor with a high-sensitivity and hip high-temperature operation.

• Journal of Applied Biological Chemistry
• /
• 제57권4호
• /
• pp.359-363
• /
• 2014

털부처꽃(Lythrum salicaria L.) 뿌리는 실온에서 80% MeOH로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. 2,2-dipicryl-1-phenylhydrazyl (DPPH) radical 소거능을 이용한 항산화 활성을 측정하여 활성 추적 분획(Activity guided fractionation)에 따라 항산화 활성이 높은 EtOAc 분획에 대하여 silica gel 및 octadecyl $SiO_2$ column chromatography를 반복하여 항산화 활성을 나타내는 화합물 1과 major 화합물 2 및 3을 분리, 정제하였다. NMR, IR, 및 MS 등의 spectrum을 해석하여, Myricetin-3-O-${\beta}$-D-glucopyranoside (1), oleanolic acid (2) 및 betulinic acid (3)로 구조를 결정하였다. 화합물 1은 DPPH radical 소거능을 이용한 항산화 활성을 측정 한 결과 $25{\mu}g/mL$ 농도에서 $74.47{\pm}1.64%$의 저해 값을 가지는 것으로 나타났다.

• Journal of Genetic Medicine
• /
• 제17권2호
• /
• pp.68-72
• /
• 2020

Purpose: Trisomy 21, the cause of Down syndrome (DS) with various medical problems, is the most common aneuploidy during the fetal period. For diagnosis, a non-invasive screening test using maternal blood, which cannot be confirmed and invasive confirmation test with a risk of miscarriage, may be performed. The trophoblast retrieval and isolation of the cervix (TRIC) have been proposed by some researchers as an alternative to overcome the limitations of current tests. We experimented using TRIC to identify the possibility of trisomy 21 for the first time in Asia. Materials and Methods: Three cases of DS were analyzed confirmed by invasive tests (chorionic villus sampling, amniocentesis). All samples of trophoblasts immediately were immersed in phosphate-buffered saline and processed with formalin for fixation. The trophoblasts were isolated using an anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. β-human chorionic gonadotropin (hCG)-expressing cells were considered as trophoblast cells, and the detection rate calculated. DS was confirmed by fluorescence in situ hybridization (FISH). Results: The mean trophoblast detection rate using β-hCG was 78.1%, and the detection rate using FISH was 22.2%. In all cases, the trisomy of chromosome 21 was identified. Conclusion: Trophoblast can be obtained from the five weeks of gestation and has a high detection rate, so it is noted that it can replace the current prenatal genetic test. To realize the clinical application as a prenatal genetic test, we will need additional efforts to identify trisomy 21 as well as other chromosomal abnormalities in future large-scale studies.

• 한국축산식품학회지
• /
• 제40권5호
• /
• pp.852-859
• /
• 2020

The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14 d-old pigs of three breeds [Landrace×Yorkshire×Duroc (LYD), Berkshire, and Korean native pigs] and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 30% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for cluster of differentiation 56 (CD56) and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56⁺/CD29⁺ muscle stem cells was significantly increased (91.5±2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.

• 한국자기공명학회논문지
• /
• 제6권1호
• /
• pp.69-77
• /
• 2002

During the screening of material which has the antimicrobial activity against aminoglycoside-resistant bacteria, A new material $\varepsilon$-(L-$\beta$-Iysine) polypeptide from a culture medium of Streptomyces sp.(DWGS2) was isolated, and the structure and the physicochemical properties of the new material were elucidated. The new material was separated by column chromatography of the culture medium using Dowex1$\times$2, Silica gel, and Sephadex LH20 etc. The chemical structure and molecular weight were determined with the data of various NMR experiments, MALDI mass, and ESI mass experiments. The antimicrobial activity of $\varepsilon$-(L-$\beta$-Iysine) polypeptide is not only better than equal to the activity of known aminoglycoside type of antibiotics(MIC=3.125 - 6.25ug/mL) but also effective against aminoglycoside-resistant bacteria and fungi. If the mechanism of antimicrobial activity against aminoglycoside- resistant bacteria is figured out, the $\varepsilon$-(L-$\beta$-Iysine) polypeptide can be utilized for the treatment of diseases caused by aminoglycoside-resistant bacteria.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2001년도 추계학술대회논문집 II
• /
• pp.1144-1149
• /
• 2001

In this paper, a sliding mode controller of a fluid engine mount using MR (Magneto-Rheological) fluid was discussed When the MR fluid is applied to a fluid mount, resistance of MR fluid can be controlled by electro-magnetic valve installed in the inertia track. Since the rheological property of the MR fluid shows a function of shear rate, the damping characteristics of the mount will be change according to the frequency. Changing an applied magnetic field to the valve changes the property of the mount, such as the resistance of the MR fluid, the notch and the resonant frequencies due to the fluid passing, quantity of the fluid passing, the effective piston area of the volumetric damping and stiffness. Therefore, the fluid mount using MR fluid can be regarded as a variable structure system The sliding mode control known well as a particular type of variable structure control was introduced in this study. The sliding mode control, which has inherent robustness, is also expected to improve the control performance in the engine mount The sliding mode controller for the mount formatted by taking into account the response property with a time constant to MR fluid and the variable mount property. The motion equations of the fluid mount are derived from Newton's law of motion and used in numerical simulation. Numerical simulations illustrate the effectiveness of the sliding mode controller.