Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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Isolation, structure elucidation and physicochemical properties of novel antibiotic polypeptide, $\varepsilon-(L-\beta-Iysine)$ polypeptide from Streptomyces sp. DWGS2

  • Donghyuk Shin (Department of Chemistry and Applied Chemistry, College of Science and Technology, Hanyang University) ;
  • Kim, Daesung (Department of Chemistry and Applied Chemistry, College of Science and Technology, Hanyang University) ;
  • Lee, Deoggeun (Central Research Laboratories, Dong-Wha Pharm. Ind. Co., LTD) ;
  • Lee, Hyeongkyu (Natural Product Biosynthesis Research Unit, Korea Research Institute of Bioscience ＆ Biotechnology) ;
  • Hoshik Won (Department of Chemistry and Applied Chemistry, College of Science and Technology, Hanyang University)
  • Published : 2002.06.01
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Abstract

During the screening of material which has the antimicrobial activity against aminoglycoside-resistant bacteria, A new material $\varepsilon$-(L-$\beta$-Iysine) polypeptide from a culture medium of Streptomyces sp.(DWGS2) was isolated, and the structure and the physicochemical properties of the new material were elucidated. The new material was separated by column chromatography of the culture medium using Dowex1$\times$2, Silica gel, and Sephadex LH20 etc. The chemical structure and molecular weight were determined with the data of various NMR experiments, MALDI mass, and ESI mass experiments. The antimicrobial activity of $\varepsilon$-(L-$\beta$-Iysine) polypeptide is not only better than equal to the activity of known aminoglycoside type of antibiotics(MIC=3.125 - 6.25ug/mL) but also effective against aminoglycoside-resistant bacteria and fungi. If the mechanism of antimicrobial activity against aminoglycoside- resistant bacteria is figured out, the $\varepsilon$-(L-$\beta$-Iysine) polypeptide can be utilized for the treatment of diseases caused by aminoglycoside-resistant bacteria.

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