• The Korean Journal of Malacology
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• v.31 no.2
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• pp.93-101
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• 2015

The effects of different stocking densities on the growth and survival rate of the 3-year-old pacific abalone, Haliotis dicus hannai were investigated in marine net cage for a year. Stocking densities in net cage ($2.4{\times}1.2m$) was set 15, 30, 45 and 60 percentage (= per)/sq m (square meter, $m^2$) with share to cross-sectional area per shelter. The water temperature during the testing period was $8.2^{\circ}C-22.1^{\circ}C$, and salinity is $33.5{\pm}0.6psu$, and dissolved oxygen is $7.87{\pm}0.86mg/L$. In the shell length (initial size : $71.50{\pm}2.28mm$) growth and shell breadth (initial size : $46.43{\pm}2.28mm$) of the test abalones, the absolute growth rate (ARG), daily growth rate (DGR) and specific growth rates (SGR) of the 15 per/sq m and 30 per/sq m were higher than those of 45 per/sq m and 60 per/sq m density group (P < 0.05). Also in the weight (initial weight : $35.7{\pm}8.1g$), it showed the same results. In survival rates, it were that 15 per/sq m and 30 per/sq m is significantly higher than 45 per/sq m and 60 per/sq m. Therefore, it was that the 15 per/sq m is optimized stocking density in marine net cages about the 3-year-old pacific abalone over 70 mm size. The result shown that total cross-sectional area under the shelter is based on 15 per/sq m ($2.4{\times}2.4m$, 354 number in a net cage) is suitable for fast growth and survival. But if the economy consider, optimized stocking density would be appropriate to accept 30 per/sq m ($2.4{\times}2.4m$, 710 number in a net cage).

• Journal of Korean Library and Information Science Society
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• v.23
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• pp.363-404
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• 1995

This study is to set up a model of minimum and optimum standards for college and university library building areas in Korea. The results of this study are summarized as follows: 1. minimum standards(proposal) At first, Areas needed by factors of space component are as follows: User space --- 0.45 $m^{2}$ per student. Collection space --- 0.0107 $m^{2}$ per volume Staff space --- 10.1 $m^{2}$ per person Space attached to user, collection and staff space --- 5% of the sum of user, collection and staff areas(0.041 $m^{2}$ per student). Nonassignable space --- 25% of the sum of user, collection and staff areas (0.21 $m^{2}$ per student). Next, the formula to calculate the total area of the college and university library building is as follows: N = 0.45T $m^{2}$(a) + 0.0107V $m^{2}$(b) + 10.1S $m^{2}$(c) + 0.05(a+b+c) $m^{2}$, NS = 0.25N $m^{2}$. 2. Optimum standards(proposal) At first, Areas needed by factors of space component are as follows: User spae --- 0.64 $m^{2}$) per student. Collection space --- 0.01 $m^{2}$ per volume Staff space --- 9.7 $m^{2}$ per person Space attached to user, collection and staff space --- 5% of the sum of user, collection and staff areas(0.073 $m^{2}$ per student). Nonassignable space --- 25% of the sum of user, collection and staff areas(0.38 $m^{2}$ per student). Next, the formula to calculate the total area of the college and university library building is as follows: N = 0.64T $m^{2}$(a) + 0.01V $m^{2}$(b) + 9.7S $m^{2}$(c) + 0.05(a+b+c) $m^{2}$, NS = 0.25N $m^{2}$.

• Molecules and Cells
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• v.22 no.3
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• pp.275-284
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• 2006

The molecular components that generate and maintain circadian rhythms of physiology and behavior in mammals are present both in the brain (suprachiasmatic nucleus; SCN) and in peripheral tissues. Examination of mice with targeted disruptions of either mPer1 or mPer2 has shown that these two genes have key roles in the SCN circadian clock. Here we show that loss of the clock gene mPer2 affects forced locomotor performance in mice without altering muscle contractility. A proteomic analysis revealed that the anterior tibialis muscles of the mPer2 knockout mice had higher levels of glycolytic enzymes such as triose phosphate isomerase and enolase than those of either the wild type or mPer1 knockout mice. In addition, the level of expression of HSP90 in the mPer2 mutant mice was also significantly higher than in wildtype mice. These results suggest that the reduced locomotor endurance of the mPer2 knockout mice reflects a greater dependence on anaerobic metabolism under stress conditions, and that the two canonical clock genes, mPer1 and mPer2, play distinct roles in the physiology of skeletal muscle.

• Animal cells and systems
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• v.9 no.3
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• pp.153-160
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• 2005

Most living organisms exhibit the circadian rhythm in their physiology and behavior. Recent identification of several clock genes in mammals has led to the molecular understanding of how these components generate and maintain the circadian rhythm. Many reports have implicated the photic induction of either mPer1 or mPer2 in the hypothalamic region called the suprachiasmatic nucleus (SCN) to phase shift the brain clock. It is now established that peripheral tissues other than the brain also express these clock genes and that the clock machinery in these tissues work in a similar way to the SCN clock. To determine the role of the two canonical clock genes, mPer1 and mPer2, in the peripheral clock shift, stable HEK293EcR cell lines that can be induced and stably express these proteins were prepared. By regulating the expression of these proteins, it could be shown that induction of the clock genes, either mPer1 or mPer2 alone is not sufficient to cause clock phase shifting in these cells. Our real-time PCR analysis on these cells indicates that the induction of mPER proteins dampens the expression of the clock-specific transcription factor mBmal1. Altogether, our present data suggest that mPer1 and mPer2 may not function in clock shift or take part in differential roles on the peripheral circadian clock.

• Journal of the Korean Institute of Educational Facilities
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• v.13 no.3
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• pp.48-55
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• 2006

This paper considered the situation of the school building facilities in the university classified by the campus characteristics. Data were collected from 224 universities campus of 180 4 year colleges. The results are as follows: 1) The floor area of school building facilities increases continuously in 2001-2003 in the campuses studied. The floor area of school building facilities per 1 student increase $15.85m^2$ in 2001, $17.62m^2$ in 2002, and $17.79m^2$ in 2003. 2) The floor area of school building facilities is distributed widely, also the floor area of school building facilities is below $130,000m^2$ and the floor area per 1 student is below $23m^2$ in 75% of the campuses studied. 3) The floor area of school building facilities per 1 student increases $16.07m^2$ in 2001, $17.41m^2$ in 2002 and $17.62m^2$ in 2003. 4) The floor area of school building facilities per 1 student is large but tends to decrease every year in the university of education. Medical, arts and physical type campus has the large floor area per 1 student and the distribution of area is wide owing to the campus having a hospital. The floor area per 1 student is large and the distribution of area is wide in the sub campus.

• The Korean Journal of Malacology
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• v.30 no.3
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• pp.219-226
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• 2014

The effects of different stocking densities on the growth and survival rate of the abalone, Haliotis discus hannai, were investigated in marine net cage for two years. Stocking density was set 15, 30, 45 and 60 percentage $(=per.)/m^2$ with share to cross-sectional area per shelter. The primary rearing period (PRP) and the secondary rearing period (SRP) were conducted by a year. One year mean water temperature of PRP and SRS showed the difference about $2^{\circ}C$. In the growth (initial mean shell length of abalone : $36.14{\pm}2.28mm$) of PRP, the absolute growth rate (ARG), daily growth rate (DGR) and specific growth rate (SGR) of the $15per./m^2$ were higher than those of density groups (P < 0.05). Survival rates of all density groups were showed no significant difference. In the growth (mean shell length of abalone : $55.26{\pm}6.93mm$) of SRP, ARG, DGR and SGR of stocking density groups showed no significant difference except for $45per./m^2$ density group. Survival rate in the low-density (15, $30per./m^2$) was more than 70%, and those of the high-density (45, $60per./m^2$) were less than 31% and 9%, respectively. These results showed that the appropriate stocking density for $15per./m^2$ was seven hundred fifty number per one net cage ($2.4{\times}2.4m$), during PRP using 3-4 cm abalone in length. Also for the secondary rearing period, the optimal stocking density (shell length 5-6 cm of abalone) consider with the economical efficiency was determined to be $30per./m^2$, resulting the productivity improved.

• Biomolecules & Therapeutics
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• v.19 no.3
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• pp.308-314
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• 2011

It has been shown that circadian genes not only play an important role on circadian rhythms, but also participate in other physiological and pathological activities, such as drug dependence, cancer development and radiation injury. The Per2, an indispensable component of the circadian clock, not only modulates circadian oscillations, but also regulates organic function. In the present study, we applied mPER2-upregulated NIH3T3 cells to reveal the relationship of mPer2 and the cells damaged by ultraviolet C (UVC). NIH3T3 cells at the peak of the expression of mPer2 induced by phorbol 12-myristate 13-acetate (PMA) demonstrated little damage by UVC evaluated by MTT assay, cell growth curves and cell colony-forming assay, compared with that at the nadir of the expression of mPer2. Overexpression of mPER2, accompanied p53 upregulated, also demonstrated protective effect on NIH3T3 cells damaged by UVC. These results suggest that mPer2 plays a protective effect on cells damaged by UVC, whose mechanism may be involved in upregulated p53.

• Journal of Life Science
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• v.27 no.5
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• pp.501-508
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• 2017

Bone morphogenetic proteins (BMPs) are multifunctional cytokines that play important roles in a variety of cellular functions. Among BMP family members, BMP2 efficiently promotes osteoblast differentiation through Smad-mediated runt-related transcription factor 2 (Runx2) expression. Several recent studies suggest that BMPs are associated with clock genes, in particular Bmal1. Bmal1 protein heterodimerizes with Clock protein and then induces period 1 (Per1) expression. However, the role of Per1 on osteoblast differentiation remains unclear. In this study, we investigated whether Per1 is involved in osteoblast differentiation. MC3T3-E1 cells were treated with BMP2 for induction of osteoblastic differentiation. Osteogenic maker gene and Per1 mRNA expression were measured using real-time PCR. Interestingly, BMP2 treatment induced Per1 mRNA expression in MC3T3-E1 cells. To further investigate the function of Per1 on osteoblast differentiation, MC3T3-E1 cells were transiently transfected with pCMV-Per1. Per1 overexpression increased Runx2 mRNA and protein levels. Also, mRNA expression and promoter activity of osteocalcin were upregulated by Per1 overexpression. To investigate the effect of interaction between Per1 and osteogenic condition, MC3T3-E1 cells were cultured in osteogenic medium containing ascorbic acid and ${\beta}$-glycerophosphate. Osteogenic medium-induced ALP staining level and mineralization were synergistically increased by overexpression of Per1. Taken together, these results demonstrate that Per1 is a positive regulator of osteoblast differentiation.

• Journal of Cadastre & Land InformatiX
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• v.45 no.2
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• pp.217-235
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• 2015

The study has analyzed the determinants of retail rents for the franchised coffee shop with the Multi-Regression analysis in Seoul. Central place, agglomeration, and demand-externality theories provide the basis for empirical models. The data used in this study consisted of 2,000 retail units in Seoul. As the results, the results show that coffee shop of retail sales per $3.3m^2$ and characteristic of retail trade-areas, and characteristic of coffee shop affects on the coffee shop's rents per $3.3m^2$. Coffee Shop's sales are the major determinant of coffee shop's rents. Also, coffee shop's goodwill per $3.3m^2$ and rental bond per $3.3m^2$ are positively correlated with a coffee shop's rents. Additionally, number of wholesale company and manufacturers in the retail trade-areas influences coffee shop's rent per $3.3m^2$. In summary, the study shows that coffee shop's brand identity, location and retail mix in the retail trade areas affects a coffee shop's rent per $3.3m^2$.

• Journal of Animal Environmental Science
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• v.5 no.3
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• pp.149-156
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• 1999

The experiment was conducted to determine the optimum stocking density for Korean Native Chicken. The experiment was carried out with 810 Korean Native Chickens for 16weeks from April 22. 1987 to August 11. 1987. The chickens were housed in pens with varying stocking densities; T1(20 birds per 3.3$m^2$), T2(30 birds per 3.3$m^2$), T3(40 birds per 3.3$m^2$), T4(50 birds per 3.3$m^2$), T5(60 birds per 3.3$m^2$) and T6(70 birds per 3.3$m^2$). Each treatment contained three replicates. At the end of the trial, the average body weight of T1 was significantly heavier than that of T5 (P<0.05), but there were no statistical differences among the treatments in fed intake. The feed conversion of T3 was improved significantly in comparison with that of T5(P<0.05), and the viability of T1 showed a significantly difference with that of T5, T1 showed the highest production number, whereas T5 the lowest one.