• 대한의생명과학회지
• /
• 제12권1호
• /
• pp.53-56
• /
• 2006

The suprachiasmatic nucleus (SCN) of the anterior hypothalamus is the circadian pacemaker entrained to the 24-hr day by environmental time cues. Major circadian genes such as mPeriod ($mPer1{\sim}3$) and mCryptochrome ($mCry1{\sim}2$) are actively transcribed by the action of CLOCK/BMAL heterodimers, and in turn, these are being suppressed by the mPER/mCRY complex. In the study, the locomotor activity rhythms of mPer1 Knockout (KO) mice are measured, and the expression profiles of Heat Shock Protein 105kDa (HSP 105) genes in the SCN were measured by in situ hybridization. In agreement with previous reports, the locomotor activity rhythm of mPer1 KO mice was much shorter than that of wildtype. In addition, the total bout of activity of mPer1 KO was less in comparison to control mice. The expression of HSP 105 in the SCN of mPer1 KO mice was ranged from CT6 to CT22, with a peak level at CT14, implying that the gene are under the control of circadian clock. However, the expression of HSP 105 in the SCN of wildtype could not be detected in our study. Further analysis will reveal the direct or indirect regulation by mPer1 on the expression in the SCN and the role of the gene in the circadian clock.

• 한국패류학회지
• /
• 제31권2호
• /
• pp.93-101
• /
• 2015

본 연구는 3년산 북방전복을 해상가두리에서 1년 동안 수용밀도별로 양식순기에 따라 사육하여 성장 및 생존율 조사를 통해 적정 수용밀도를 구명하고 생산성을 향상시키고자 수행하였다. 해상가두리 ($2.4{\times}1.2m$) 내 수용밀도는 셀터 단면적 (square meter, $m^2$, sq m) 당 점유율로 15, 30, 45, 60 percentage (= per)/sq m로 설정하여 2반복으로 실시하였다. 사육기간 동안 수온은 $8.2^{\circ}C-22.1^{\circ}C$, 염분은 평균 $33.5{\pm}0.6psu$, 용존산소는 평균 $7.87{\pm}0.86mg/L$이었다. 월별 각장변화에서는 2014년 5월, 7월부터 10월, 2015년 4월에 15 per/sq m, 30 per/sq m가 45 per/sq m, 60 per/sq m보다 유의적으로 높았다 (P < 0.05). 각장의 FML, AGR, DGR, SGR과 각폭의 FMB, AGR, DGR은 15 per/sq m와 30 per/sq m는 45 per/sq m, 60 per/sq m보다 유의적으로 높았고 (P < 0.05), 각폭의 SGR에서 60 per/sq m는 모든 실험구 보다 낮았다 (P < 0.05). 중량에 대한 FMW, WG, DWG, SWG에서도 같은 결과를 나타내었다 (P < 0.05). 생존율에서는 15 per/sq m, 30 per/sq m가 50%이상으로 45 per/sq m, 60 per/sq m 보다 유의적으로 높았다 (P < 0.05). 따라서, 본 실험결과 70 mm 이상의 3년산 북방전복의 해상가두리 내 적정 수용밀도는 셀터 총 단면적 기준으로 15 per/sq m ($2.4{\times}2.4m$, 1칸 당 354마리) 가 빠른 성장과 생존율을 위한 적정 수용밀도지만 경제성을 고려할 경우 30 per/sq m ($2.4{\times}2.4m$, 1칸 당 710마리) 수용해야 한다.

• Animal cells and systems
• /
• 제9권3호
• /
• pp.153-160
• /
• 2005

Most living organisms exhibit the circadian rhythm in their physiology and behavior. Recent identification of several clock genes in mammals has led to the molecular understanding of how these components generate and maintain the circadian rhythm. Many reports have implicated the photic induction of either mPer1 or mPer2 in the hypothalamic region called the suprachiasmatic nucleus (SCN) to phase shift the brain clock. It is now established that peripheral tissues other than the brain also express these clock genes and that the clock machinery in these tissues work in a similar way to the SCN clock. To determine the role of the two canonical clock genes, mPer1 and mPer2, in the peripheral clock shift, stable HEK293EcR cell lines that can be induced and stably express these proteins were prepared. By regulating the expression of these proteins, it could be shown that induction of the clock genes, either mPer1 or mPer2 alone is not sufficient to cause clock phase shifting in these cells. Our real-time PCR analysis on these cells indicates that the induction of mPER proteins dampens the expression of the clock-specific transcription factor mBmal1. Altogether, our present data suggest that mPer1 and mPer2 may not function in clock shift or take part in differential roles on the peripheral circadian clock.

• Molecules and Cells
• /
• 제22권3호
• /
• pp.275-284
• /
• 2006

The molecular components that generate and maintain circadian rhythms of physiology and behavior in mammals are present both in the brain (suprachiasmatic nucleus; SCN) and in peripheral tissues. Examination of mice with targeted disruptions of either mPer1 or mPer2 has shown that these two genes have key roles in the SCN circadian clock. Here we show that loss of the clock gene mPer2 affects forced locomotor performance in mice without altering muscle contractility. A proteomic analysis revealed that the anterior tibialis muscles of the mPer2 knockout mice had higher levels of glycolytic enzymes such as triose phosphate isomerase and enolase than those of either the wild type or mPer1 knockout mice. In addition, the level of expression of HSP90 in the mPer2 mutant mice was also significantly higher than in wildtype mice. These results suggest that the reduced locomotor endurance of the mPer2 knockout mice reflects a greater dependence on anaerobic metabolism under stress conditions, and that the two canonical clock genes, mPer1 and mPer2, play distinct roles in the physiology of skeletal muscle.

• 한국도서관정보학회지
• /
• 제23권
• /
• pp.363-404
• /
• 1995

This study is to set up a model of minimum and optimum standards for college and university library building areas in Korea. The results of this study are summarized as follows: 1. minimum standards(proposal) At first, Areas needed by factors of space component are as follows: User space --- 0.45 $m^{2}$ per student. Collection space --- 0.0107 $m^{2}$ per volume Staff space --- 10.1 $m^{2}$ per person Space attached to user, collection and staff space --- 5% of the sum of user, collection and staff areas(0.041 $m^{2}$ per student). Nonassignable space --- 25% of the sum of user, collection and staff areas (0.21 $m^{2}$ per student). Next, the formula to calculate the total area of the college and university library building is as follows: N = 0.45T $m^{2}$(a) + 0.0107V $m^{2}$(b) + 10.1S $m^{2}$(c) + 0.05(a+b+c) $m^{2}$, NS = 0.25N $m^{2}$. 2. Optimum standards(proposal) At first, Areas needed by factors of space component are as follows: User spae --- 0.64 $m^{2}$) per student. Collection space --- 0.01 $m^{2}$ per volume Staff space --- 9.7 $m^{2}$ per person Space attached to user, collection and staff space --- 5% of the sum of user, collection and staff areas(0.073 $m^{2}$ per student). Nonassignable space --- 25% of the sum of user, collection and staff areas(0.38 $m^{2}$ per student). Next, the formula to calculate the total area of the college and university library building is as follows: N = 0.64T $m^{2}$(a) + 0.01V $m^{2}$(b) + 9.7S $m^{2}$(c) + 0.05(a+b+c) $m^{2}$, NS = 0.25N $m^{2}$.

• 생명과학회지
• /
• 제27권5호
• /
• pp.501-508
• /
• 2017

Bone morphogenetic proteins (BMPs)는 다양한 세포기능을 조절하는 중요한 사이토카인 중 하나이다. 최근 BMP와 일주기 유전자들이 연관되어 있다는 연구결과들이 보고되고 있지만 조골세포에서 일주기 유전자인 Per1의 역할은 아직 명확하지 않다. 본 연구에서는 조골세포 분화에서 Per1의 역할을 조사하였다. MC3T3-E1 세포에서 BMP2 처리에 의해 Per1 mRNA 발현과 luciferase 활성이 증가하는 것을 확인하였다. 또한 Per1 과발현 실험을 통해서 Per1 유전자가 Runx2, ALP, OC의 발현을 증가시켰으며 ascorbic acid와 ${\beta}$-glycerophosphate에 의한 ALP 염색과 석회화가 Per1 과발현에 의해 더욱 증가하는 것을 확인하였다. 이상의 결과는 일주기 리듬을 조절하는 Per1 유전자가 조골세포의 분화를 촉진하는 인자로 작용함을 시사한다.

• 교육시설
• /
• 제13권3호
• /
• pp.48-55
• /
• 2006

This paper considered the situation of the school building facilities in the university classified by the campus characteristics. Data were collected from 224 universities campus of 180 4 year colleges. The results are as follows: 1) The floor area of school building facilities increases continuously in 2001-2003 in the campuses studied. The floor area of school building facilities per 1 student increase $15.85m^2$ in 2001, $17.62m^2$ in 2002, and $17.79m^2$ in 2003. 2) The floor area of school building facilities is distributed widely, also the floor area of school building facilities is below $130,000m^2$ and the floor area per 1 student is below $23m^2$ in 75% of the campuses studied. 3) The floor area of school building facilities per 1 student increases $16.07m^2$ in 2001, $17.41m^2$ in 2002 and $17.62m^2$ in 2003. 4) The floor area of school building facilities per 1 student is large but tends to decrease every year in the university of education. Medical, arts and physical type campus has the large floor area per 1 student and the distribution of area is wide owing to the campus having a hospital. The floor area per 1 student is large and the distribution of area is wide in the sub campus.

• 한국축산시설환경학회지
• /
• 제5권3호
• /
• pp.149-156
• /
• 1999

The experiment was conducted to determine the optimum stocking density for Korean Native Chicken. The experiment was carried out with 810 Korean Native Chickens for 16weeks from April 22. 1987 to August 11. 1987. The chickens were housed in pens with varying stocking densities; T1(20 birds per 3.3$m^2$), T2(30 birds per 3.3$m^2$), T3(40 birds per 3.3$m^2$), T4(50 birds per 3.3$m^2$), T5(60 birds per 3.3$m^2$) and T6(70 birds per 3.3$m^2$). Each treatment contained three replicates. At the end of the trial, the average body weight of T1 was significantly heavier than that of T5 (P<0.05), but there were no statistical differences among the treatments in fed intake. The feed conversion of T3 was improved significantly in comparison with that of T5(P<0.05), and the viability of T1 showed a significantly difference with that of T5, T1 showed the highest production number, whereas T5 the lowest one.

• 대한의생명과학회지
• /
• 제11권4호
• /
• pp.493-501
• /
• 2005

Circadian rhythms, time on a scale of about 24 hours, are present in a number of organisms including animals, plants, and bacteria. The control of the biochemical, physiological and behavioral processes is regulated by endogenous clocks in the suprachiasmatic nucleus (SCN). At the core of this timing mechanism is molecular machinery that are present both in the brain and in the peripheral tissues throughout the body, and even in a single cultured cell. In this study, we performed two-dimensional gel electrophoresis to figure out any correlation between protein expression patterns and the requirement of two canonical clock proteins, either mPER1 or mPER2, by comparing global protein expression profiles in livers from wildtype or mPer1/mPer2 double mutant mice. We could identify several differentially expressed protein candidates with respect to time and genotypes. Further analysis of these candidate proteins in detail in vivo will lead us to the better understanding of how circadian clock functions in mammals.

• 한국방사선학회논문지
• /
• 제11권5호
• /
• pp.321-328
• /
• 2017

D-Shuttle (Chiyoda Technol Corporation, Tokyo, Japan) 선량계를 이용하여 개인피폭관리 및 자연방사선량의 모니터링을 위한 기초자료를 제공하는데 연구의 목적이 있다. D-Shuttle을 이용하여 선량을 산출하였다. 선량보고서에서 400 일 노출되었을 때에 1.346 mSv 이었고, 연간선량 (annual dose per year)은 1.228 mSv/year, 평균시간선량 (average dose per hour)은 $0.014{\mu}Sv/hr$ 이었다. 국내의 개인 외부피폭선량 (1.295 mSv/year =Korea average natural individual external dose), 국내의 연간부가선량 (additional dose per year)은 -0.0663 mSv/year 이다. D-Shuttle은 방사선모니터링을 위한 개인선량계로 방사선의 검출성능 우수한 기능, 실시간 방사선 피폭관리, 방사선 작업의 경보 기능, 효율적이고 사용이 편리한 개인 방사선선량의 피폭관리로 ALARA에 매우 유용한 선량계로 사용할 수 있다. 방사선작업종사자와 지역주민의 방사선모니터링 측정기기로 병원, 산업, 의료현장, 원전사고 지역과 비파괴 분야의 위험한 지역에서 방사선모니터링으로 활용될 수 있다.