• Title/Summary/Keyword: mMT

Search Result 1,178, Processing Time 0.03 seconds

Analyses of Hydrology and Groundwater Level Fluctuation in Granite Aquifer with Tunnel Excavation (터널 굴착에 의한 화강암 대수층의 수리 수문 및 지하수위변동 분석)

  • Chung, Sang-Yong;Kim, Byung-Woo;Kang, Dong-Hwan;Shim, Byoung-Ohan;Cheong, Sang-Won
    • The Journal of Engineering Geology
    • /
    • v.17 no.4
    • /
    • pp.643-653
    • /
    • 2007
  • Average hydraulic conductivity was $2.64{\times}10^{-8}m/sec$ average RQD was 78%, average porosity was 0.51%, and range of groundwater level was $77.06{\sim}125.97m$ by measured in 8 boreholes at the Surak Mt. tunnel area. Groundwater level of two peaks in the Surak Mt. tunnel area were estimated through linear regression analysis for groundwater level versus elevation. And, average horizontal hydraulic gradient in the Surak Mt. tunnel area was calculated 0.267. Minimum, maximum, and average hydraulic conductivities that estimated by field tests were $5.56{\times}10^{-9}m/sec,\;6.12{\times}10^{-8}m/sec,\;and\;2.64{\times}10^{-8}m/sec$, respectively. Groundwater discharge rates per 1 meter that estimated using minimum, maximum, and average hydraulic conductivities and average horizontal hydraulic gradient were $0.00585m^2/day,\;0.06434m^2/day,\;and\;0.02775m^2/day$, respectively. Pure groundwater recharge rate per unit recharge area was calculated 223.96 mm/yr through water balance analysis. Prediction simulation of groundwater level fluctuation with minimum, maximum, and average hydraulic conductivities were conducted. Discharge rate into the Surak Mt. tunnel for minimum hydraulic conductivity was small, but groundwaer drawdown was highly. Discharge rate into the Surak Mt. tunnel for maximum hydraulic conductivity was higher, but groundwaer level was recovered quickly.

Expression of Vitellogenin Gene by Androgens in Rasinbow Trout, Oncorhynchus mykiss (웅성호르몬에 의한 무지개송어의 vitellogenin 유전자 발현)

  • 권혁추;윤종만;이종영
    • Journal of Aquaculture
    • /
    • v.13 no.1
    • /
    • pp.79-85
    • /
    • 2000
  • The effects of estrogen and androgens on Vg gene expression were examined in primary hepatocyte culture and livers of the immature male trout. Specific primers of Vg cDNA were designed with already reported Vg gene nucleotide sequences. PCR product was sequenced and verified with Vg cDNA of rainbow trout. Total RNA was extracted from the cultured hepatocytes and livers of steroid-treated rainbow trout and then it was analyzed by reverse transcriptase- polymerase chain reaction (RT-PCR) analysis. The Vg mRNA and Vg protein synthesis were increased in rainbow trout in vivo and in vitro with E$_2$ and methyltestosterone (MT) There were dose and time-related effects of E$_2$ and MT on vitellogesis. Androgens such as progesterone androsterone and testosterone also stimulated Vg mRNA expression in vitro. The results show that androgens as well as E$_2$ can induce expression of Vg mRNA in trout in vivo and in vitro.

  • PDF

Cytoprotective Effect by Antioxidant Activity of Codonopsis lanceolata and Platycodon grandiflorum Ethyl Acetate Fraction in Human HepG2 Cells (인간 HepG2 세포에서 더덕 및 도라지 에틸아세테이트 분획물의 항산화 효과에 의한 세포보호 효과)

  • Kim, Soo-Hyun;Choi, Hyun-Jin;Oh, Hyun-Taek;Chung, Mi-Ja;Cui, Cheng-Bi;Ham, Seung-Shi
    • Korean Journal of Food Science and Technology
    • /
    • v.40 no.6
    • /
    • pp.696-701
    • /
    • 2008
  • The objective of this study was to determine whether Codonopsis lanceolata or Platycodon grandiflorum ethyl acetate fraction (CLEA or PGEA) protect cells against sodium nitroprusside (SNP)-induced oxidative stress via the expression of various antioxidant systems. The HepG2 cells exposed for 24 hr to 0.5 mM SNP showed a reduction in the cell viability by an MTT assay. Pretreatment with CLEA and PGEA resulted in an inhibition of SNP-induced cell death. In addition, the effects of CLEA and PGEA on the expression of antioxidant systems via RT-PCR analyses was assessed. The levels of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD) and metallothionein (MT)-1A mRNA were increased after 24 hr of CLEA exposure. The levels of Mn superoxide dismutase CAT, G6PD, MT-1A, and MT-2A mRNA were increased after PGEA treatment. In conclusion, CLEA and PGEA exert indirect antioxidant effects, perhaps via the induction of a variety of antioxidant systems which, may protect cells against oxidative stress.

Induction of Vitellogenin Synthesis by Androgens in Cultured Hepatocytes of the Eel, Anguilla japonica (간세포 배양을 이용한 뱀장어 Vitellogenin 합성에 대한 웅성호르몬의 영향)

  • 권혁추;박홍양
    • Korean Journal of Animal Reproduction
    • /
    • v.20 no.3
    • /
    • pp.259-269
    • /
    • 1996
  • To establish whether or not androgens is responsible for the induction of vitellogenin(Vg) synthesis and secretion, primary hepatocytes prepared from immature eels were used. The results are follows: 1. Eel hepatocytes were prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in medium for 10 days with minimal cell loss. 2. Estradiol-17$\beta$(E2) alone was insufficient to induce Vg synthesis. The combination of E2 with methyltestosterone(MT) markedly stimulated Vg synthesis. High vg production occurred in MT concentration from 10-6~10-5M in the presence of E2 (10-6M). Testosterone and androsterone were also effective, but progesterone was not effective in inducing Vg synthesis. Neither MT alone nor testosterone and androsterone alone had any effect on Vg synthesis. 3. E2-primed hepatocytes showed Vg synthesis in both media with and without hormones 1 day after culture. In the cultures with the vehicle, MT, or progesterone, the rate of synthesis seemed to decrease with time. But the combination of E2 and MT showed an intense increase in Vg synthesis. Hepatocytes isolated from E2-primed eels also required androgens for continuating of Vg synthesis. 4. These results demonstrate that androgens act together with E2 in synthesis and secretion of eel Vg.

  • PDF

The Energy Flow and Mineral Cycles in a Zoysia japonica and a Miscanthus sinensis Ecosystem on Mt. Kwanak 8. The Cycles of Sulphur (관악산의 잔디와 억새 생태계에 있어서 에너지 흐름과 무기물의 순환 8.황의 순환)

  • 강경미;장남기
    • Asian Journal of Turfgrass Science
    • /
    • v.11 no.4
    • /
    • pp.281-288
    • /
    • 1997
  • This study was performed to find out the mineral cycles of sulphur in dynamic grassland ecosystems in a steady state condition at the northwest side on Mt. Kwanak. The experimental results may be summarized on the communities of a Zoysia japonica and a Miscanthus sinensis as follows. As compared with some properties of the surface soils among two semi-natural grasslands, sulphur war greater quantity in a Miscanthus sinensis than in a Zoysia japonica on Mt. Kwanak . For the case of steady production and release, the raion of annual mineral production to the amount accumulated on the top of mineral soil in a steady state provides the estimates of release constant k. The release constants on sulphur of the litter were 0.54 in a Zoysia japonica and 0.36 in a Miscanthus sinensis grassland. The half times of S required for the release or accumulation of the litter on the grassland were 1.28 years in a Zoysia japonica and 1.93 years in a Miscauthus sinensis The amounts of annual cycles for sulphur in the grassland ecosystem under the steady-state conditions were 20.65g /$m^2$ in a Zoysia japonica and 26.28g /$m^2$ in a Miacanthus sinensis grassland. Key words: Zoysia japonica Miscanthus sinensis Mt. Kwanak, Sulphur, Mineral cycles.

  • PDF

Effects of glycyrrhizinic acid, menthol and GA: Mt (2: 1), GA: Mt (4: 1) and GA: Mt (9: 1) supramolecular compounds on mitochondrial functional activity IN VITRO experiments.

  • L. A., Еttibaeva;U. K., Abdurahmonova;A.D., Matchanov;S., Karshiboev
    • Journal of Integrative Natural Science
    • /
    • v.15 no.4
    • /
    • pp.137-144
    • /
    • 2022
  • This paper presents the effect of the supramolecular complex of GA (Glycyrrhizic acid) and Mt(menthol) and GA: Mt (4: 1) obtained on their basis can restore functional dysfunction of the liver mitochondria in alloxan diabetes, ie, inhibit lipid peroxidation. The hypoglycemic activity and mitochondrial membrane stabilizing properties of the supramolecular compound GA: Mt (4: 1) in alloxan diabetes were more pronounced than those of menthol, GA and its GK: Mt (2: 1) and GA: Mt (9: 1) compounds. According to the results obtained, the concentration of GA did not affect the peroxidation of lipid membranes of the liver mitochondria. However, a concentration of 15 μM of GA was found to reduce LPO (lipid peroxidation) formed by the effect of Fe2+ / ascorbate on the mitochondrial membrane by 58.0 ± 5.0% relative to control. The inhibitory effect of GA and its supramolecular compounds in different proportions with menthol on the peroxidation of lipids in rat heart and brain tissue has been studied

Soil Respiration Characteristics in Pinus densiflora Forests in Republic of Korea: A Case of Mt. Jeombongsan, Mt. Namsan, and Mt. Jirisan (2009~2010) (우리나라 소나무림의 토양호흡 특성: 점봉산, 남산, 지리산 사례 연구 (2009~2010))

  • Jae-Ho Lee;Young-Ju Yu;Sang-Hun Lee;Man-Seok Shin;Jae-Seok Lee
    • Korean Journal of Ecology and Environment
    • /
    • v.56 no.4
    • /
    • pp.440-448
    • /
    • 2023
  • This study measured soil respiration in pine forests dominated by Pinus densiflora in Mt. Jeombong, Mt. Namsan, Mt. Jirisan in Republic of Korea from 2009 to 2010. The seasonal variations, along with temperature and soil moisture content, were measured to understand the characteristics at each site. Soil respiration was highest in summer and autumn, closely influenced by the increase in soil temperature. Throughout the measurement period, soil respiration ranged from 205.6 to 312.2 mg CO2 m-2 h-1, with Mt. Namsan showing the highest values and Mt. Jirisan the lowest. A strong correlation was observed between soil respiration and soil temperature, with Q10 values ranging from 2.5 to 3.0. Precipitation significantly affected soil moisture content, and although it appeared to influence soil respiration, no significant correlation was found.

THE FLORA OF BRYOPHYTES ON MT. KWANAK, WITH SOME NEW ADDITIONS TO THE KOREAN FLORA (미기록종을 포함한 관악산의 선태류)

  • Hong, Won-Shic
    • Journal of Plant Biology
    • /
    • v.3 no.2
    • /
    • pp.19-25
    • /
    • 1960
  • 1. This study was made on the flora of bryophytes on Mt. Kwanak, which is situated ($37^{\circ}$30' N. Lat. $127^{\circ}$O'E. Long) to the south of Seoul area. 2. The bryophytic flora of this mountain consists of the following elements. 1) Holarctic elem, Musci………16 species (25.0%), Hepaticae………4 species (28.6%). 2) North Pacific elem. M.………1 species (1.56%), H………O. 3) Cosmopolitan elem. M………10 species (15.65%), H………1 species (7.1%). 4) East-Asiatic elem. M………16 species (25%), H.………4 species (28.6%). 5) Tropical elem. M.………3 species (4.68%), H.………3 species (21.4%). 6) Japane-Korean elem. M.………17 species (26.56%), H.………2 species (14.3%). 7) Endemic elem. M.………1 species (1.56%), H.………O. 3. In the list of bryophytes of Mt. Kwanak, 62 species, 1 variety and 1 subspecies belonging to 47 genera for Musci and 12 species 2 subspecies belonging to 10 genera for Hepaticae were enumerated. Of these the following 5 species are new to Korean flora. 1) Macromitrium japonicum D.M. 2) Entodon arenosus Okam. 3) E. okamurae Broth. 4) Gollania ruginosa (Mitt.) Broth. 5) Temnona birmensis (St.) Hatt.

  • PDF

송백류의 분포를 중심으로 하는 한라산의 수식분포대

  • 엄규백
    • Journal of Plant Biology
    • /
    • v.5 no.2
    • /
    • pp.17-20
    • /
    • 1962
  • Altitudinal zones of Mt. Hanla were geoecologically investigated, and compared with climate index, according to Coniferae distribution. For climatic index, Warmth index was calculated on Mt. Hanla. With the resultsobtained, the altitudinal zones can be classified into the following three zones; Coniferous forest zone: below $43^{\circ}$ (month-degrees) (above 1, 450m) Deci(u)us broad-leaf forest zone: $84^{\circ}$-$43^{\circ}$ (600-1, 450m) Lucidophyllous forest zone: above $84^{\circ}$ (below 600m)

  • PDF

Effects of Acute Metal Exposures on the Viability and mRNA Expression of Metallothionein in Hemibarbus mylodon Fry

  • Bang, In-Chul;Cho, Young-Sun;Lee, Il-Rho;Nam, Yoon-Kwon
    • Korean Journal of Ecology and Environment
    • /
    • v.40 no.4
    • /
    • pp.489-494
    • /
    • 2007
  • Transcriptional modulation of metallothionein (MT) during acute metal exposures (cadmium, copper or zinc) was examined in fry of Hemibarbus mylodon, a threatened fish species in Korean peninsula. Viability of H. mylodon fry was most affected by copper exposure (up to 79% of mortality at 1 ppm for 48 hours) and considerably by cadmium exposure (21 to 54% of mortality). On the other hand, Zn showed the least adverse effect on the viability (0 to 13% of mortality) of this species. Based on the semi-quantitative RT-PCR analysis, the stimulation of MT mRNA in response to metal exposures followed generally in a dose-dependent fashion where cadmium was the most potent inducer for the induction of MT transcripts in fry (up to more than 5-fold) while the lowest response was observed in zinc-exposed group (2-fold at maximum). From the exposure using environmentally realistic doses of cadmium (0 to 0.05 ppm for 24 hours), MT expression at mRNA level was also sensitively modulated toward upregulation up to more than 3-fold as relative to non-exposed control. Results from the present study would be a good basis for understanding the adaptive capacity and stress physiology of this endangered fish species during metal pollution.