• Journal of the Korean Electrochemical Society
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• v.10 no.4
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• pp.301-305
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• 2007

The performance of polymer electrolyte membrane fuel cell could be decreased due to coolant leaked from connection part. Micro pump was used to put small amount of coolant and investigate the effect on fuel cell. The stoichiometric ratio of hydrogen/air was 1.5/2.0, both side of gas was fully humidified, and current density of $400mA/cm^2$ was used as standard condition in this experiment. Constant current method was used to check performance recovery from coolant effect in 3 cell stack. The performance was recovered when coolant was injected in cathode side. On the other hand, the performance was not recovered when coolant was injected in anode side. Ethylene glycol could be converted to CO in oxidation process and cause poisoning effect on platinum catalyst or be adhered on GDL and cause gas diffusion block effect resulting performance decrease. Water with nitrogen gas was supplied in anode side to check performance recovery. Polarization curve, cyclic voltammetry, electrochemical impedance spectroscopy was used to check performance, and gas chromatography was used to check coolant concentration. Constant current method was not enough in full recovery of performance. However, water injection method was proved good method in full recovery of performance.

• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.1-10
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• 2008

The seasonal variations of water quality and periphyton were investigated in an artificial stream (the Cheonggyecheon Stream) flowing through the Seoul City. TP showed a longitudinal gradient: 10 to $59{\mu}gP\;L^{-1}$ in the upper stream sites, and 15 to $90{\mu}gP\;L^{-1}$ in downstream sites. POP was a major form of TP in the water, occupying over 60%, while the proportion of DIP was less than 10% except for St. 4. N/P atomic ratio ranged from 78 to 554, which implies phosphorus would limit algal growth more than nitrogen. The biomass of periphyton did not show much difference among sites, and it was relatively higher in spring and fall season $(10\sim20{\mu}gChl\;{\alpha}cm^{-2})$ and lower in August $(<5{\mu}gChl\;{\alpha}cm^{-2})$, possibly because biofilms were washed off during spates of summer monsoon. Cyanobacteria was the dominant taxon in the periphyton community throughout the year. The periphyton standing crop can be classified as a nuisance level. It seems that phosphorus level is sufficiently high even though the input water is treated chemically, and modest water velocity $(20\sim90cm\;sec^{-1})$ and rocky bottom provide optimal conditions for periphyton growth.

• Korean Journal of Ecology and Environment
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• v.34 no.1 s.93
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• pp.30-44
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• 2001

In this study limnological characteristics of Lake Juam was surveyed from June 1993 to May 1994 in order to provides important information regarding water resources. Secchi disc transparency, epilimnetic chlorophyll a (chi-a), total nitrogen (TN), total phosphorus (TP) concentration and primary productivity were in the range of $2.0{\sim}4.5\;m$, $0.9{\sim}13.6\;mgChl/m^3$, 0.78$\{sim}$2.32 mgN/l, $11{\sim}56\;mgP/m^3$, $270{\sim}2.160\;mgCm^{-2}\;day^{-1}$, respectively. On the basis of TP, Chl-a and Secchi disc depth, the trophic state of Lake Juam can be classied as mesotrophic lake. The phosphorus inputs from non-point sources are concentrated in heavy rain episodes during the monsoon season. As a result, phosphorus concentration are higher in summer than in winter. TP loading from the watershed were estimated to be $0.9\;gPm^{-2}yr^{-1}$, which correspond to a boundary of the critical loading ($1.0\;gPm^{-2}yr^{-1}$) for eutrophication. From the results of the algal assay, both phosphous and nitrogen act as limiting nutrients in algal growth. The seasonal succession of phytoplankton community structure in Lake Juam was similar to that observed in other temperate lakes. Diatoms (Asterionella formosa and Aulacoseira granulate var. angustissima)fujacofeira BraHuJafa uar. aHgusHrsiaia) weredominant in spring and winter, cyanobacteria) were dominant in warm season. The organic carbon, nitrogen and phosphorus content of lake sediment were $9.5{\sim}14.0\;mgC/g$, $1.01{\sim}1.82\;mgN/g$ and $0.51{\sim}0.65\;mgP/g$, respectively. The allochthonous organic carbon loading from the watershed and autochthonous organic carbon loading by primary production of phytoplankton were determined to be 1,122 tC/yr and 6,718 tC/yr, respectively. To prevent eutrophication of Lake Juam, nutrient management of watershed should be focus on reduction of fertilizer application, proper treatment of manure, and conservation of topsoil as well as point source.

• Korean Journal of Ecology and Environment
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• v.38 no.4 s.114
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• pp.461-474
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• 2005

Physicochemical parameters, plankton community structure, and sediment were surveyed from 1988 to 2002, at two months interval, in a eutrophic coastal lagoon (Lake Songji, Korea). The lake basin is separated from the sea by a narrow sand dune, and a shallow sill divides the lake basin into two sub-basins. The stable stratifications and chemoclines are maintained all through the year at 1-2 m depth. DO was often very low (<1 $mgO_2\;{\cdot}\;L^{-1}$) in the monimolimnion. Secchi disc transparency was in the range of 0.5-2.7 m. TP, TN, and Chl. a concentration in the mixolimnion were 0.015-0.396 $mgP\;{\cdot}\;L^{-1}$), 0.223-3.521 $mgN\;{\cdot}\;L^{-1}$, and 0.5-129.8 mg ${\cdot}\;m^{-3}$, respectively. TSI was in the eutrophic range of 54 to 62. Sediment was composed of silt and coarse silt. COD, TP, and TN content of the sediment were 51.4-116.9 $mgO_2\;{\cdot}\;gdw^{-1}$, 0.04-1.46 $mgP\;{\cdot}\;gdw^{-1}$ and, 0.12-1.03 $mgN\;{\cdot}\;gdw^{-1}$, respectively. The 49 phytoplankton species were identified. The maximum phytoplankton abundance obscured the lake in September 2001 (max. density: 23,350 cells ${\cdot}\;mL^{-1}$. The Chlorophyte Schroederia judayi was dominant species in summer (max. density: 20,417 cells ${\cdot}\;mL^{-1}$). The lake showed unique limnological features of a brackish lagoon in respect to biological community, chemical characteristics, and physical phenomena.

• Korean Journal of Ecology and Environment
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• v.34 no.1 s.93
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• pp.20-29
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• 2001

The amount, chemical composition and optical property of extracellular dissolved organic carbon (EOC) by phytoplankton were examined using axenic cultures of Microcystis aeruginosa, Anabaena flos-aquae, and Oscillatoria agardhii. The extracellular organic matter was categorized into five fractions (hydrophobic acids; AHSs, hydrophobic neutrals; HoNs, hydrophilic acids; HiAs, hydrophilic bases; HiBs, and hydrophilic neutrals; HiNs) using three adsorbent resins(XAD-8, cation, and anion). The release pattern and chemical composition of EOC varied with algal species and their growth phases. Percentage of extracellular release increased with age in all cultures. HiAs were the dominant component of EOC in all cultures, whereas the proportion of HiAs decreased with age in all cultures. In contrast, the proportions of HiBs and HiNs increased as cultures aged. In particular, the HiN fraction increased from 0% to 44% of EOC in M. aeruginosa and from 3.0% to 28% in A. flos-aquae, respectively. The proportion of AHSs was higher in the cultures of A. flos-aquae(7.5${\sim}$16%) and O. agardhii (8.7${\sim}$16%) than M. aeruginosa(0.2${\sim}$2.5%). The proportions of AHSs increased with culture age in M. aeruginosa and O. agardhii, but decreased in A. flos-aquae. The specific UV absorbance also varied among species; 1.9 Abs${\cdot}$cm$^{-1}$/mgC${\cdot}$L$^{-1}$ for M. aeruginosa, 3.7 Abs${\cdot}$cm$^{-1}$/mgC${\cdot}$L$^{-1}$ for A. flos-aquae, and 13.0 Abs${\cdot}$cm$^{-1}$/mgC${\cdot}$L^{-1}$ for O. agardhii. The results of this study indicates that DOC excreted by three blue-green algae differed with species and the growth phase.

• Korean Journal of Ecology and Environment
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• v.37 no.1 s.106
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• pp.12-25
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• 2004

Physicochemical parameters, plankton biomass, and sediment were surveyed from 1998 to 2000 at two months interval in a eutrophic coastal lagoon(Lake Hwajinpo, Korea). The lake is separated from the sea by a narrow sand dune. Littoral zone is well vegetated with leafing-leaved aquatic plants. The lake basin is divided into two subbasins by a shallow sill. It has intrusion of seawater by permeation and stormy waves. Stable chemoclines are formed by salinity difference at 1m depth all the year round. DO was often very low (< 1 mg$O_2\;L^{-1}$) at hypolimnion. Temperature inversions were observed in November. Nitrate and ammonium concentrations were very low(< (1.1 mgN $L^{-1}$), even though TN was usually 2.0 ${\sim}$ 3.5 mgN $L^{-1}$. TN/TP was generally lower than the Redfield ratio. Transparency was 0.2 ${\sim}$ 1.7 m, and COD, TP, and TN of sediment were 3.1 ${\sim}$ 40.3 mg$O_2\;g^{-1}$, 0.91 ${\sim}$ 1.39 mgP $g^{-1}$, and 0.34 ${\sim}$ 3.07 mgN $g^{-1}$, respectively. Phytoplankton chlorophyll- a concentrations were mostly over 40 mg $m^{-3}$. Two basins showed different phytoplankton communities with Oscillatoria so., Trachelomonas sp., Schizochlamys gelatinosa, and Anabaena spiroides dominant in South basin, and with Trachelomons sp., Schroederia so., schizochlamys gelatinosa, and Trachelomonas sp. dominant in the North basin. The seasonal succession of phytoplankton was very fast, possibly due to sudden changes in physical conditions, such as wind, turbidity, salinity and light.

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.75-80
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• 2001

Purpose: There was little evidence that Tc-99m labeled ciprofloxacin (Infecton) located inside of bacteria. Antimicrobial activity of Infecton could be an indirect evidence of its location. We compared in vitro antimicrobial activities of Infecton and ciprofloxacin. Materials and methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Infecton and ciprofloxacin against three standard strains of bacteria, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were measured using modified broth macrodilution techniques and pour plate methods, respectively. Data were expressed as mean${\pm}$SE (range). Results: MICs of Infecton and ciprofloxacin were $1.12{\pm}0.20\;(0.8{\sim}1.6){\mu}g/ml\;and\;0.35{\pm}0.04\;(0.2{\sim}0.4){\mu}g/ml$ for S. aureus, $0.03{\pm}0.005\;(0.025{\sim}0.05){\mu}g/ml\;and\;0.011{\pm}0.001\;(0.006{\sim}0.012){\mu}g/ml$ for E. coil, and $0.96{\pm}0.16\;(0.8{\sim}1.6){\mu}g/ml)\;and\;0.56{\pm}0.098\;(0.4{\sim}0.8){\mu}g/ml$ for P. aeruginosa, respectively. MBCs of Infecton and ciprofloxacin were $2.56{\pm}0.39\;(1.6{\sim}3.2){\mu}g/ml\;and\;0.88{\pm}0.2\;(0.4{\sim}1.6){\mu}g/ml$ for S. aureus, $0.04{\pm}0.05\;(0.025{\pm}0.05){\mu}g/ml\;and\;0.02{\pm}0.01\;(0.025{\sim}0.05)\;{\sim}g/ml$ for E coli, and $2.24{\pm}0.39\;(1.6{\sim}3.2){\mu}g/ml\;and\;1.44{\pm}0.16\;(0.8{\sim}1.6){\mu}g/ml$ for P. aeruginosa, respectively. Conclusion: Although both MICs and UBCs of Infecton were higher than those of ciprofloxacin, all three standard bacterial strains were sensitive to Infecton. It could be an indirect evidence that Tc-99m Infecton be a specific imaging agent for bacterial infection.

• The Korean Journal of Nuclear Medicine
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• v.34 no.6
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• pp.456-464
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• 2000

Purpose: To evaluate the effect of acupuncture on regional cerebral blood flow (rCBF) at acupoints suggested by oriental medicine to be related to the treatment of cerebrovascular diseases. Materials and Methods: Rest/acupuncture-stimulation Tc-99m ECD brain SPECT using a same-dose subtraction method was performed on 54 normal volunteers (34 males, 20 females, age range from 18 to 62 years) using six paradigms: acupuncture at acupoints GV. 20, GV. 26, LI. 4, ST. 36 and SP. 6. In the control study, needle location was chosen on a non-meridian focus 1 cm posterior to the right fibular head. All images were spatially normalized, and the differences between rest and acupuncture stimulation were statistically analyzed using SPM$^{(R)}$ for Windows$^{(R)}$. Results: Acupuncture applied at acupoint GV. 20 increased rCBF in both the anterior frontal lobes, the right frontotemporal lobes, and the left anterior temporal lobe and the left cerebellar hemisphere. Acupuncture at GV 26 increased rCBF in the left prefrontal cortex. Acupuncture at LI. 4 increased rCBF in the left prefrontal and both the inferior frontal lobes, and the left anterior temporal lobe and the left cerebellar hemisphere. Acupuncture at ST. 36 increased rCBF in the left anterior temporal lobe, the right inferior frontal lobes, and the left cerebellum. Acupuncture at SP. 6 increased rCBF in the left inferior frontal and anterior temporal lobes. In the control stimulation, no significant rCBF increase was observed. Conclusion: The results demonstrated a correlation between stimulation at each acupoint with increase in rCBF to the corresponding brain areas.

• The Korean Journal of Nuclear Medicine
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• v.30 no.1
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• pp.95-103
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• 1996

Reverse redistribution(RRD) refers to a perfusion defect that develops or becomes more evident on rest imaging compared with the stress imaging. This phenomenon was not uncommonly noted on myocardial perfusion single photon emission computed tomography (SPECT). However, the clinical significance and pathophysiological mechanism of RRD were unclear. The aim of this study was to evaluate the incidence and clinical significance of RRD on either dipyridamole T1-201 or Tc-99m MIBI myocardial perfusion SPECT. RRD was defined as ${\geq}10%$ decrease in relative T1-201 and Tc-99m MIBI uptakes on rest images compared to the stress images or as an appearance of new perfusion defects on rest images. It was observed in both T1-201 (44/463, 9.5%) and Tc-99m MIBI (124/999, 12.4%) myocardial SPECTs similarly, with an overall incidence of 11.5%(168/1462). Many apparent)y unrelated disease groups showed the finding: post-revascularization(53.9%), coronary artery disease(24.6%), myocardial infarction(12.3%), and those with normal coro-nary arteries (9.2%). Clinical and angiographic characteristics of 65 consecutive patients who underwent coronary arteriography in 168 patients who had RRD on myocardial perfusion SPECT were reviewed. Tc-99m MIBI was used in 44 patients, and T1-201 was used in 21 patients. Of the 81 myocardial segments analyzed which showed RRD, 32 segments(39.5%) were in septum, 24(29.5%) in inferior wallL, 12(14.8%) in anterior wall, 7(8.7%) in apex and 6(7.4%) in lateral wall. There was no clear association between RRD and coronary arterial stenosis or Presence of collateral circulations. Ventriculographical wall motion was evaluated in 27 regions with RRD; it was normal in 12 regions, hypokinetic in 12 regions and dyskinetic in 3 regions. In 14 of 21 patients who showed RRD on T1-201 myocardial SPECT, T1-201 reinjection was performed immediately after the 3-4 hour redistribution studies. Ten of 14 (71.4%) showed enhanced T1-201 activity(${\geq}10%$ increased) after reinjection. We conclude that RRD is not related to mode of stress or radiopharmaceuticals. RRD might represent many inhomogeneous pathophysiological processes.

• The Korean Journal of Nuclear Medicine
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• v.35 no.1
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• pp.33-42
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• 2001

Purpose: The purpose of this study was to evaluate the effect of scatter correction on the assessment of myocardial perfusion and left ventricular function by gated Tc-99m myocardial SPECT. Materials and Methods: Subjects were 11 normal volunteers, 20 patients with non-cardiac chest pain and 13 patients with coronary artery diseases. We classified above 3 groups into normal and diseased groups. Scatter correction was done using dual-energy-window scatter correction method (DEW-SC). We compared acquired counts, image contrast, corrected maximum relative counts, indices of left ventricular function, extent and severity of perfusion defects calculated by 'CEqual program' between scatter non-corrected and corrected images. Results: Scatter corrected studios was lower in counts by $18{\pm}3%$ than uncorrected studies, but image contrast were improved in all cases. Scatter correction using DEW-SC took 3 minutes to complete, and 512 kB memory to store. There were no significant differences among indices of left ventricular function between scatter non-corrected and corrected images. Although extents of perfusion defects were not significantly different, severity was severer in scatter corrected images. Conclusion: Scatter correction using DEW-SC is simple to do, and improves image contrast without changing other indices of myocardial perfusion and function.