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Optimization of Synthesis Condition of Monolithic Sorbent Using Response Surface Methodology (반응 표면 분석법을 이용한 일체형 흡착제의 합성 조건 최적화)

  • Park, Ha Eun;Row, Kyung Ho
    • Applied Chemistry for Engineering
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    • v.24 no.3
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    • pp.299-304
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    • 2013
  • A 17-run Box-Behnken design was used to optimize the synthesis conditions of a monolithic sorbent. The effects of the amount of monomer (mL), crosslink (mL) and porogen (mL) were investigated. The experimental data were fitted to a second-order polynomial equation by the multiple regression analysis and examined using statistical methods. The adjusted coefficient of determination ($R^2$) of the model was 0.9915. The probability value (p < 0.0001) demonstrated a high significance for the regression model. A mean amount of polymer as 2120.15 mg was produced under the following optimum synthesis conditions: the optimized volumes of monomer, crosslink and porogen are 0.30, 1.40, and 1.47 mL, respectively. This was in good agreement with the predicted model value.

Comparision of the Activity and Characteristics of Lactate Dehydrogenase Isolated from Different Parts of Soybean Seedling (발아초기의 콩 부위별 Lactate Dehydrogenase 활성변화 및 효소성질 비교)

  • Lee, Hyo-Sa;Jun, Tae-Hong
    • Applied Biological Chemistry
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    • v.26 no.1
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    • pp.28-34
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    • 1983
  • The change of lactate dehydrogenase(LDH) activity and the possibility of the existence of LDH. isozyme were examined with different parts of soybean sprout. The enzyme activity was little changed in cotyledons throughout the early stagy of germination. However, hypocotyls and roots showed the continuous decline of the enzyme activity since the radicle emerged from seeds. It was found that LDH from hypocotyls. and roots was unstable as compared with LDH from cotyledons, even at low temperature. The enzyme from hypocotyls and roots was not purified with a good yield when the purification procedure developed for LDH from cotyledons. was employed. LDH from hypocotyls and roots has the Rm value of 0.29, and 0.25 from cotyledons. The apparent Km value for LDH from cotyledons was 0.45mM with sodium pyruvate, while crude homogenate of hypocotyls or roots showed biphasic phenomenon with two Km values 0.014 and 0.45mM. The results indicate the possibility that crude homogenate of hypocotyls or roots may contain a different LDH isozyme from the LDH of soybean reported previously.

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Inhibitory Effects of Coptisine on Monoamine Oxidase Activity

  • Lee, Myung-Koo;Lee, Kyong-Soon;Kim, Hack-Seang;Hong, Seung-Soo;Ro, Jai-Seup
    • Natural Product Sciences
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    • v.6 no.2
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    • pp.70-72
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    • 2000
  • The effects of coptisine on monoamine oxidase (EC 1.4.3.4; MAO) activity in mouse brain were investigated. Coptisine showed an inhibitory effect on MAO activity with a concentration-dependent manner. Coptisine exhibited 51.0% inhibition of MAO activity at $9\;{\mu}M$. The $IC_{50}$ value of coptisine was $8.7\;{\mu}M$. Coptisine inhibited MAO activity competitively with kynuramine as a substrate. The $K_i$ value of coptisine was $4.1\;{\mu}M$. These results indicate that coptisine functions to regulate the catecholamine content at biologically active sites.

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A study for Decision Method of Human Body Safety according to Power Load Fluctuation of the Chechon Area (제천지역 전력부하변동에 따른 인체 전 판단 방법에 대한 연구)

  • 김상철;김두현
    • Journal of the Korean Society of Safety
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    • v.15 no.4
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    • pp.69-77
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    • 2000
  • This paper presents a study for decision method of human body safety according to power load fluctuation of the Chechon area. To cope with substantial electromagnetics analysis, the safety assessments were analyzed the double 154 [kV] T/L, 345 [kV] T/L, Chechon-Ichon, Chechon-Youngju, respectively. As the results of case study, in case of 345 [kV] T/L, the electric field value was 11.4927 [kV/m], magnetic field value was 0.4622 [G] at the point about 7 [m] away from the line in severest case. The results showed that the membership of a proposition fuzzy '11.4937 [kV/m] is hazardous' is 0.7063 for the severest case in case of 345 [kV] T/L. As the analytic results using the fuzzy qualifier term, the membership in case of very false is 0.0862 and fairly false is 0.5419, respectively.

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Quality Characteristics and Antioxidant Activities of Cookies containing Teff (Eragrostis tef) Flour (Teff (Eragrostis tef) 분말을 첨가한 Gluten-free 쿠키의 품질 특성 및 항산화 활성 비교)

  • Joung, Ki Youeng;Song, Ka-Young;O, Hyeonbin;Zhang, Yangyang;Shin, So Yeon;Kim, Young-Soon
    • The Korean Journal of Food And Nutrition
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    • v.30 no.3
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    • pp.501-509
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    • 2017
  • This study investigated the quality characteristics and antioxidant activities of gluten-free cookies containing teff flour. By substituting 0% (control), 25% (TF25), 50% (TF50), 75% (TF75), and 100% (TF100) of wheat flour with teff flour, five samples were produced. Baking loss rate was the highest in TF25 at 13.76% and the lowest in TF75 at 4.03%. Spread factor was significantly higher in cookies made with teff flour (83.00~85.00) than in the control (81.33) (p<0.05). There was no significant difference in density among the samples at 1.17~1.25 g/mL (p<0.05); however, pH significantly decreased at 6.42~6.04 (p<0.05). While the L-value and b-value significantly decreased with the amount of teff flour, the a-value significantly increased (p<0.05). The ${\Delta}E$ value was the highest in the control at 31.31 and the lowest in TF100 at 58.69. Hardness was the highest in the control at 42.04 N than in cookies containing teff flour. The content of polyphenols was the highest in TF100 at $3.37{\mu}g\;GAE/mg$ and the lowest in the control at $1.32{\mu}g\;GAE/mg$. The content of flavonoids was the highest in TF100 at $3.66{\mu}g\;QE/mg$ and the lowest in controls at $0.45{\mu}g\;QE/mg$. The value of DPPH $IC_{50}$ was the highest in the control at $3,723.00{\mu}g/mL$ and the lowest in TF 100 at $405.27{\mu}g/mL$. The value of ABTS $IC_{50}$ was the highest in the control at $1,822.32{\mu}g/mL$ and the lowest in TF100 at $529.30{\mu}g/mL$. In sensory evaluation, while control, TF75, and TF100 had a higher score in appearance at 5.52~5.60, all samples had no significant differences in flavor, sweetness, savory taste, chewiness, and overall acceptability (p<0.05). These results showed that the gluten-free cookies containing teff flour can improve the quality characteristics and antioxidant activities of a cookie. We concluded that gluten-free cookies containing 100% teff flour are desirable.

Effect of Various Gluten-free Flours on Quality Characteristics and Antioxidant Activities of Cookies (전분의 종류를 달리하여 제조한 Gluten-free 쿠키의 품질특성 및 항산화 활성 비교)

  • Joung, Ki Youeng;Song, Ka-Young;O, Hyeonbin;Zhang, Yangyang;Shin, So Yeon;Kim, Young-Soon
    • Korean journal of food and cookery science
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    • v.33 no.2
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    • pp.127-136
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    • 2017
  • Purpose: This study examined the effect of different gluten-free flours on the properties of cookies. Methods: Each gluten-free cookie was made from wheat, brown rice, buckwheat, corn, sorghum, teff, or black rice. Results: Baking loss was highest on sorghum (18.85%) and the lowest on teff (12.73%). The spread factor was highest on brown rice (8.20) and lowest on corn (7.60). The density was similar in all samples (1 g/mL). pH was the highest on buckwheat (6.45) and lowest on wheat (5.96). While L-value, a-value and b-value were lowest on black rice, the ${\Delta}E-value$ was the highest on black rice (35.57). The hardness was highest on wheat (30.28 N) and lowest on teff (14.87 N). The polyphenol and flavonoid content was the highest on buckwheat ($25.97{\mu}g\;GAE/mg$) and the flavonoid content was the highest on black rice ($24.33{\mu}g\;QE/mg$). The DPPH IC50 value was highest on wheat ($352.41{\mu}g/mL$) and lowest on black rice ($33.59{\mu}g/mL$). The ABTS $IC_{50}$ value was highest on wheat ($349.30{\mu}g/mL$) and lowest on black rice ($57.72{\mu}g/mL$). The results of the sensory properties revealed color to be highest on corn (7.33). The top grain was similar in all the samples (5.43-6.57). Flavor was highest on black rice and teff (6.43-6.48). The softness was the highest on wheat, sorghum, teff, and black rice (6.48-7.05). Sweetness was highest on sorghum, teff, and black rice (6.19-6.71). The overall preference also was highest on sorghum, teff, and black rice (6.14-6.48). Conclusion: Gluten-free flours can improve the quality characteristics and antioxidant activities of cookies.

A Survey of Direct Normal Insolation Resources for the Construction of Solar Thermal Power Generation Sites in Korea (국내 태양열발전단지 건설을 위한 법선면 직달일사량 자원조사)

  • Jo, Dok-Ki;Kang, Young-Heack;Auh, Chung-Moo
    • Journal of the Korean Solar Energy Society
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    • v.25 no.1
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    • pp.11-18
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    • 2005
  • Since the direct normal insolation is a main factor for designing any solar thermal power system, it is necessary to evaluate its characteristics all over the country. We have begun collecting direct normal insolation data since December 1990 at 16 different locations and considerable effort has been made for constructing a standard value from measured data at each station. KIER(Korea Institute of Energy Research's new data will be extensively used by solar thermal concentrating system users or designers as well as by research institutes. From the results, we can conclude that 1) Yearly mean $5.4kWh/m^2/day$ of the direct normal insolation was evaluated for clear day all over 16 areas in Korea. 2) Clear day's direct normal insolation of spring and summer were $5.53kWh/m^2/day$ and $5.84kWh/m^2/day$, and for fall and winter their values were $5.3kWh/m^2/day$ and $4.94kWh/m^2/day$ respectively. So, spring and summer were higher, and fall and winter were lower than the yearly mean value.

Serum Alpha-Fetoprotein Levels in Health Checkup Adults (건강 검진자들의 혈청 Alpha-Fetoprotein 농도)

  • Kim, Yoohyun
    • Korean Journal of Clinical Laboratory Science
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    • v.40 no.2
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    • pp.86-93
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    • 2008
  • This study was performed to investigation of the serum alpha-fetoprotein (AFP) levels in healthy adults. A total of 2,160 (male 1,415, female 745) health checkup adults were examined for AFP levels by chemiluminescence immunoassay (CLIA) method, during the period from September, 2007 to August, 2008. The mean serum AFP level was 2.168 (0.605~20.35) ng/mL, and it was 2.309 (0.605~20.35) ng/mL in male, 1.906 (0.605~10.36) ng/mL in female, respectively. 1,816 (male 1,109, female 709) healthy adults were screened for the absence of viral hepatitis and normal alanine amino transferase (ALT) levels. The mean serum AFP level of healthy adult was 2.041 (0.605~7.83) ng/mL, and it was 2.181 (0.605~7.83) ng/mL in male, 1.822 (0.605~6.55) ng/mL in female, respectively. Serum AFP increased with age group, there was a higher level in male compared to female. These results suggests that the use of reference value of AFP in healthy adults in the Jeonbuk. With the reference value now defined, it becomes possible to compare levels in different populations.

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Morphometric Study on Fine Needle Aspirates from Follicular Adenoma and Follicular Carcinoma of the Thyroid (세침흡인된 갑상선의 여포상 선종과 여포상 암종의 형태계측학적 연구)

  • Chu, Young-Chae;Cha, Hee-Jung;Min, Soo-Kee;Kim, Joon-Mee;Hwang, Tae-Sook
    • The Korean Journal of Cytopathology
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    • v.9 no.1
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    • pp.63-68
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    • 1998
  • Fine needle aspiration cytology of "cold" nodules of the thyroid has proved to be of great value in their preoperative diagnosis. Most types of thyroid tumors are readily recognizable from characteristic cellular patterns in the smears of needle aspirates. But follicular neoplasms present some problems because the cytomorphology of the adenomas frequently is same as in carcinoma. For differentiation of benign from malignant follicular neoplasms of the thyroid we tested the usefulness of two objective parameters - nuclear area and perimeter - by morphometry. This study was made on fine needle aspirates from 30 cases with cytologic diagnosis of follicular neoplasm of thyroid. The histologic classification was follicular adenoma in 22 cases and follicular carcinoma in 8 cases. As a reference group we used seven caes with nodular hyperplasia. The smears of aspirates were stained by Papanicolaou method. On each slide 200 randomly selected cells with intact nuclei were measured. The mean value of nuclear area are $25.32{\pm}5.50{\mu}m^2,\;34.08{\pm}7.50{\mu}m^2\;and\;39.97{\pm}6.63{\mu}m^2$ in nodular hyperplasia, follicular adenoma, and follicular carcinoma, respectively. The mean value of perimeter are $19.48{\pm}2.26{\mu}m,\;22.95{\pm}2.65{\mu}m\;and\;24.78{\pm}2.23{\mu}m$ in nodular hyperplasia, follicular adenoma and follicular carcinoma, respectively. The mean nuclear areas and perimeters of cells from follicular adenoma were significantly larger than those from nodular hyperplasia (p<0.05). The mean nuclear areas and perimeters of cells from follicular carcinoma were larger than those from follicular adenoma but the differences are not significant statistically(p>0.05). Therefore, morphometric assessment alone is inadequate to predict malignancy in thyroid aspirates.

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Evaluation of Bacteriological Safety for the Shellfish growing Sea waters in Seocheon Area, Korea (충남 서천 패류생산해역에서의 세균학적 위생안전성 평가)

  • Byun, Han-Seok;Song, Ki-Cheol;Lee, Doo-Seog;Shim, Kil-Bo;Lim, Chi-Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.1
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    • pp.25-32
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    • 2010
  • A Sanitary survey in an Important shellfish-growing area of Biin Bay in Seocheon-gun Korea, was conducted to evaluate bay conditions and compliance with the bacteriological criteria for areas designated for the production of shellfish for export. Seawater samples were collected monthly at 55 sampling stations established in the survey area from January 2006 to December 2008. Bacteriological water quality did not change in response to a small rainfall (10.0 mm), but it increased abruptly in response to rainfall to 62.5 mm The most probable number (MPN) value for total coliforms and fecal coliforms in 1, 980 seawater samples ranged from <1.8 to >1, 600 MPN/100mL and from <1.8 to 330 MPN/100mL, respectively. The geometric mean and estimated $90^{th}$ percentile value for total coliforms ranged from 2.0 to 10.3 MPN/100mL and from 7.0 to 42.6 MPN/100mL respectively. The geometric mean and estimated percentile value for fecal coliforms ranged from 1.8 to 4.0 MPN/100mL and from 1.9 to 18.3 MPN/100mL, respectively. Accordingly, the bacteriological water quality of Biin Bay met the National Shellfish Sanitation Program (NSSP) and Korea Shel1fish Sanitation Program(KSSP) criteria for areas designated for shellfish production for export in Korea.