• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.87-87
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• 2001

도살장에서 구입한 소의 난소로부터 회수한 미성숙 난포란을 체외에서 성숙, 수정시킨 후, 2~8세포기 수정란을 CR$_1$aa 체외배양액에 일정량의 aesculetin, taurine을 첨가하여 체외배양을 실시한 두 처리구간의 항산화 효과를 비교 검토하고, aesculetin과 taurine에 growth factor(EGF, PDGF)를 첨가배양하여 체외수정란의 체외발육에 항산화제와 growth factor의 상호작용 효과를 검토하였다. 대조구, aesculetin(1$\mu\textrm{g}$/$m\ell$) 및 taurine(2.5mM)을 첨가하여 체외수정란을 체외배양시킨 결과 상실배이상 발육된 체외발육율은 각각 46.5%, 64.3% 및 60.5%로서 대조구보다 aesculetin과 taurine 첨가구가 유의하에 높은 성적을 얻어, aesculetin과 taurine이 체외수정란의 체외발육에 높은 효과를 나타냈다. 대조구, aesculetin (1$\mu\textrm{g}$/$m\ell$) ＋ PDGF (1ng/$m\ell$) 첨가구, aesculetin (l$\mu\textrm{g}$/$m\ell$) ＋ EGF (10ng/$m\ell$)첨가구, taurine (2.5mM) ＋PDGF (1ng/$m\ell$) 첨가구 및 taurine (2.5mM)＋EGF (10ng/$m\ell$) 첨가구에서 배반포기 발육율은 각각 46.0%, 70.0%, 58.0%, 56.0 및 66.0%로써 처리구가 무첨가구에 비해 유의하게 높은 성적을 얻어(P<0.05), 항산화제와 growth factor의 첨가 배양이 체외수정란의 체외발육에 상승효과가 있음이 입증되었다. 배양액에 천연추출 aesculetin과 상품화된 aesculetin을 첨가하여 체외배양을 실시한 결과 배반포기 발육율은 대조구, 천연추출 aesculetin 및 상품화된 aesculetin 첨가구에서 각각 38.6%, 54.6% 및 53.5%로써 첨가구가 무첨가구보다 높은 성적을 얻어, aesculetin의 항산화 효과가 입증되었다(P<0.05). 그러나 모든 처리구에서 배반포까지 발육된 체외수정란의 세포수에는 커다란 차이가 인정되지 않았다.

• Journal of Interdisciplinary Genomics
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• 제5권2호
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• pp.35-41
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• 2023

Background: Ca²⁺ signaling plays a vital role in neuronal signaling and altered Ca²⁺ homeostasis in Parkinson's disease (PD). Overexpression of αSYN significantly promote the Ca²⁺-Calmodulin (CaM) activity and subsequent nuclear translocation of nuclear factor of activated T cells (NFAT) transcription factor in dopaminergic neurons of midbrain. However, the exact role of Ca²⁺-CaM and NFAT in PD pathology is yet to be elucidated. Methods: We designed the CaM-NFAT-oligodeoxynucleotide (ODN), a synthetic short DNA containing complementary sequence for NFAT transcription factor and CaM mRNA. Then, the effect of CaM-NFAT-ODN on 1-methyl-4-phenylpyridinium (MPP⁺)-mediated neurotoxicity was investigated in mimic PD model in vitro. Results: First, the expression of αSYN and CaM was strongly increased in substantia nigra (SN) of PD and the expression of tyrosine hydroxylase (TH) was strongly increased in control SN. Additionally, the expression of apoptosis marker proteins was strongly increased in SN of PD. Transfection of CaM-NFAT-ODN repressed CaM and pNFAT, the target genes of this ODN in rat embryo primary mesencephalic neurons. It also reduced ERK phosphorylation, a downstream target of these genes. These results demonstrated that CaM-NFAT-ODN operated successfully in rat embryo primary mesencephalic neurons. Transfection of CaM-NFAT-ODN repressed TH reduction, αSYN accumulation, and apoptosis by MPP⁺-induced neurotoxicity response through Ca²⁺ signaling and mitogen-activated protein kinases (MAPK) signaling. Conclusion: Synthetic CaM-NFAT-ODN has substantial therapeutic feasibility for the treatment of neurodegenerative diseases.

• 동의생리병리학회지
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• 제36권2호
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• pp.66-72
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• 2022

Flos Lonicerae Japonicae (the flower buds of Lonicera japonica Thunberg) has been used as an antibacterial and antiviral drug in Korean Medicine. The aim of this study is to evaluate the effect of Flos Lonicerae Japonicae water extract (FL) on the production of cytokines in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). After 24 h treatment, the production of various cytokines from RAW 264.7 was measured with multiplex cytokine assay using Bio-Plex 200 suspension array system. FL at concentrations of 50, 100, and 200 ㎍/mL significantly inhibited productions of tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1β, and MIP-2 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly inhibited productions of leukemia inhibitory factor, LIX (CXCL5), and RANTES in LPS-stimulated RAW 264.7 cells; FL at concentrations of 200 ㎍/mL significantly inhibited productions of granulocyte-macrophage colony-stimulating factor and macrophage colony-stimulating factor in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50 and 100 ㎍/mL significantly increased productions of interleukin (IL)-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50, 100, and 200 ㎍/mL significantly increased productions of IL-6 and interferon gamma-induced protein-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly increased productions of monocyte chemoattractant protein-1 in LPS-stimulated RAW 264.7 cells. Taken together, these data mean that FL might modulate productions of cytokines, chemokines, and growth factor in LPS-stimulated macrophages. Further study needs to verify the exact mechanism for modulatory activities of FL with macrophages.

• 대한약리학회지
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• 제31권1호
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• pp.27-37
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• 1995

본 연구에서는 콜린성 기전에 반응하여 분비되는 내피 의존성 이완물질(endothelium-derived relaxing factor, EDRF)나 nitric oxide(NO)가 마취 흰쥐의 뇌혈류 자가조절기전에 관여할 가능성을 관찰하였다. Acetylcholine($10^{-9}-10^{-6}M$)을 포함한 mock 뇌척수액(CSF)을 관류시 뇌연막 동맥은 농도에 의존하여 이완반응 나타내었고(평균; $19.3{\pm}1.7{\mu}m$, n=36), 이러한 이완반응은 $N{\omega}$-nitro-L-arginine(L-NNA, $10^{-5}M$)에 의해서 억제되었을 뿐 아니라 methylene blue($10^{-6}M$)나 oxyhemoglobin($10^{-6}M$)에 의하여도 억제되었다. 한편 이러한 acethlcholine에 의한 뇌연막동맥의 이완반응을 매게하는 무스카린 수용체는 무스카린 수용체 길항제의 봉쇄효과를 관찰한 실험에서 $M_1$과 $M_3$ 아형으로 생각되었다. L-Arginine을 함유한 mock CSF로 관류시 일어나는 일시적인 혈관이완반응은 NY 83583 ($10^{-5}M$)에 강력히 억제되었으나 L-NNA ($10^{-5}M$)에 의해서는 억제되지 아니하였다. 한편 acetylcholine과 L-arginine에 의한 혈관이완반응은 ATP-sensitive $K^+$ 통로 봉쇄제인 glibenclamide에 의해 유의하게 봉쇄되었다. 나아가 뇌연막동맥의 직경 변화를 동맥압의 변화에 대하여 검정한 결과 혈관이완과 혈관수축의 희귀 직선의 경사도는 $10^{-5}M$ L-NNA의 전처치에 의하여 영향을 받지 아니하였으나, $3{\times}10^{-6}M$ glibenclamide에 의해 유의하게 감소되었다. 이러한 결과로 보아 혈압하강에 대해 쥐의 뇌연막동맥에 나타나는 혈관이완반응은 EDRF(NO)에 의해 매개되지 않는다고 사료된다.

• 대한전자공학회논문지SD
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• 제46권8호
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• pp.6-11
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• 2009

VA Mode LC를 사용하는 Full-HD급 LCOS 마이크로디스플레이에서 셀 상하 간격의 변화에 따른 전기광학적 특성의 변화에 관하여 3차원 LC code를 이용하여 연구하였다. 5가지의 다른 셀 상하 간격 (1.4 ${\mu}m$, 1.8 ${\mu}m$, 2.1 ${\mu}m$, 2.4 ${\mu}m$ 그리고 2.8 ${\mu}m$)의 변화에 따라 Reflectance-Voltage 특성, 반사광 및 반사율 분포, Optical Fill Factor 그리고 명암비를 비교하였다. Surface Anchoring 효과에 의해 상하 간격이 증가하면서 반사율은 증가하였으나 Optical Fill Factor와 명암비를 고려할 경우, 중간 수준의 간격인 2.1 ${\mu}m$ 의 특성이 가장 우수함을 확인할 수 있었다. 동일한 구조를 갖고 있는 0.7 인치 LCOS 패널을 제작하여 광학적 측정을 해 본 결과 시뮬레이션 결과와 통일함을 확인할 수 있었다.

• Journal of Microbiology
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• 제39권1호
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• pp.67-73
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• 2001

A validation study was conducted to evaluate the efficacy and mechanism of the cryo-precipitation, monoclonal anti-FVIIIc antibody (mAb) chromatography, Q-Sepharose chromatography, and lyophilization steps involved in the manufacture of high purity factor VIII (GreenMono) from human plasma, in the removal and/or inactivation of hepatitis A virus (HAV). Samples from the relevant stages of the production process were spiked with HAV and subjected to scale-down processes mimicking the manufacture of the high purity factor VIII concentrate. Samples were collected at each step and immediately titrated using a 50% tissue culture infectious dose (TCID$\_$50/) and then the virus reduction factors were evaluated. HAV was effectively partitioned from factor VⅢ during cryo-precipitation with the log reduction factor of 3.2. The mAb chromatography was the most effective step far removal of HAV with the log reduction factor of $\geq$4.3. HAV infectivity was not detected in the fraction of factor VⅢ, while most of HAV infectivity was recovered in the fractions of flow through and wash during mAb chromatography. Q-Sepharose chromatography showed the lowest efficacy for partitioning HAV with the log reduction factor of 0.7. Lyophilization was an effective step in inactivating HAV with the log reduction factor of 2.3. The cumulative lag reduction factor, $\geq$10.5, achieved for tile entire manufacturing process was several magnitudes greater than the potential HAV load of current plasma pools.

• 한국자기학회지
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• 제8권6호
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• pp.341-345
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• 1998

상용화된 5종류의 비정질 리본으로 제작한 실린더형 차폐체의 자기차폐효과를 미국재료시험협회(ASTM)에 준하여 연구하였다. 2705M과 2605CO를 이용한 복층 차폐인자는 2705M을 안쪽층으로 한 경우가 2605CO를 안쪽층으로 한 경우보다 상대적으로 2배 이상의 차폐효과를 얻을 수 있었다. 이는 바깥층이 2705M인 경우, 낮은 자기장세기에서 포화되는 2705M이 높은 세기의 자기장을 효과적으로 차폐하지 못하고, 1차적으로 차폐적된 자기장 세기에서 2605CO는 투자율이 낮기 때문이다. 반면 바깥층이 2605CO인 경우, 높은 자기장 세기에서 포화되는 2605CO인 높은 세기의 자기장을 효과적으로 차폐하고 1차적으로 차폐된 자기장 세기에서 2705M의 투자율은 높기 때문이다.

• 대한수학회보
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• 제23권2호
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• pp.117-121
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• 1986

Connes [2] showed that if M is an injective .sigma.-finite factor of type II $I_{1}$ and $B_{\phi}$=C1 for some normal faithful state .phi. on M then M is isomorphic to the Araki-wood factor. In [7], Haagerup has succeeded to show that if M is an injective factor of type II $I_{1}$ with separable predual, then $B_{\phi}$=C1 for every normal faithful state on M. Since injective factors of type II $I_{\lambda}$, 0.leq..lambda.<1, were classified [9], this together classifies all injective factors of type III with separable predual. It is not known for non-injective case. In this paper we consider some conditions under which the bicentralizers be trivial.ivial.

• 대한치과기공학회지
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• 제30권2호
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• pp.105-119
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• 2008

This study explored the status of free time activities among the dental technicians who are working at Seoul, Gyonggido, and Gangwondo. On the basis of this research, we aim to present needs and applicable solutions to improve the working conditions and enhance the quality of life through more effective time management in free time activities that lead to their self development and far more enriched life. 500 subjects were randomly chosen and the questionary sheets were provided. Among them, 305 sheets were returned to be analysed through SPSS Win 10.0 software and the results are as follows; First, mean in the satisfaction of leisure activities was 3.44(SD=.73). Mean in sub categories was as follows; psychological factor(M=3.65, SD=.71), social factor(M=3.54, SD=.75), rest factors(M=3.52, SD=.63), physical factor(M=3.43, SD=.88), other factors(M=3.40, SD=.66), circumstantial factor(M=3.33, SD=.63), educational factor(M=3.24, SD=.73). Second, the most relevant sub categories in the satisfaction of leisure activities were social, educational, physical, and other circumstantial factors by sequence. Factors that affect leisure activities satisfaction were career and status of health, which shows statistical significance(p<.001). That is to say, contention of this study is that the general characteristics are closely related with and affect the types and the level of satisfaction in leisure activities of the subjects. Considering these results, the dental businesses and the associations of dental technicians should recognize the needs to improve the time management through more efficient cooperations between dentists and back office dental team, systemized working time, and introduction of incentive system among others. On the basis of this appreciation, the dental business should provide more time and various opportunities with the dental technicians. Dental technicians also need to reconsider their appreciations of leisure activities, that is, leisure activities are not just spending time doing something but they refresh them and enable them to be more active and faithful to home and work.

• 한국토양환경학회지
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• 제5권3호
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• pp.65-75
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• 2001

본 연구는 제주도의 전체 지하수공 중 수역별로 대표성이 있는 150공을 선정하여 고도에 따른 수질 특성을 파악하는데 목적을 두었다. 수질특성의 평가는 물리.화학적 특성과 통계분석을 이용하였다. Piper diagram에 의한 수질 유형은 표고 50m이하 지역은 $Na^{+}$＋ $K^{+}$-C $l^{-}$ 형태를 보이고 있다. 40~100 m에 위치한 지하수에서는 $Na^{+}$＋ $K^{+}$-HC $O_3$$^{-}$ ＋C $O_3$$^{2-}$ 형태와 일부 $Na^{+}$＋ $K^{+}$-C $l^{-}$ 형태의 군을 이루고 있다. 그리고 100 m이상의 지역에서는 $Na^{+}$＋ $K^{+}$-HC $O_3$$^{-}$ ＋C $O_3$$^{2-}$ 형태에 속하고 있다. 요인분석 결과, 50 m이하와 100 m이상 지역에서 공통적으로 TDS와 오염물질 2개 요인이 추출되었다. 50~100m지역에는 3개의 요인이 추출되었으며, 요인1에는 TDS함량으로 요인2는 오염의 영향과 광물용해로 볼 수가 있다. 그리고 요인 3에는 HC $O_3$$^{-}$ 함량으로 나타났다..