• YAKHAK HOEJI
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• v.50 no.2
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• pp.105-110
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• 2006

The effects of tributyltin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with TBTA at $0.05\sim0.25{\mu}M$ significantly decreased the intracellular dopamine content in a concentration-dependent manner ($IC_{50}$ value, $0.17{\mu}M$). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by $0.1{\mu}M$ TBTA at 24 h, and recovered there-after. In addition, treatment with L-DOPA at 20 and $50 {\mu}M$ increased the intracellular dopamine content in PC12 cells and the increase in dopamine content by L-DOPA was significantly abolished by TBTA at $0.1\sim0.2{\mu}M$. These results indicate that TBTA at $0.1\sim0.2{\mu}M$ causes the decrease in the basal dopamine content and abolishes the increase in dopamine content in L-DOPA-treated cells in part by the inhibition of TH gene expression and activity.

• Korean Journal of Pharmacognosy
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• v.41 no.3
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• pp.221-226
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• 2010

The effects of sesamin on dopamine biosynthesis in PC12 cells were investigated. Sesamin at concentration ranges of 20-75 ${\mu}M$ significantly increased intracellular dopamine levels and tyrosine hydroxylase (TH) activities at 24 h: 50 ${\mu}M$ sesamin increased dopamine levels to 132% and TH activities to 128% of control levels. Sesamin (50 ${\mu}M$) induced the phosphorylation of TH, cyclic AMP-dependent protein kinase (PKA) and cyclic AMP-response element binding protein (CREB) for 0.5-24 h. Sesamin (50 ${\mu}M$) also increased the mRNA levels of TH and CREB for 3-24 h. In addition, sesamin (50 ${\mu}M$) associated with L-DOPA (50 and 100 ${\mu}M$) further increased the intracellular levels of dopamine for 24 h compared to L-DOPA alone. These results suggest that sesamin enhances dopamine biosynthesis and L-DOPA-induced increase in dopamine levels by inducing TH activity and TH gene expression, which is mediated by PKA-CREB systems in PC12 cells. Therefore, sesamin could serve as an adjuvant phytonutrient for neurodegenerative diseases.

• Korean Journal of Veterinary Service
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• v.41 no.4
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• pp.239-244
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• 2018

Mycobacterium tuberculosis (M. tuberculosis) complex is the causative agent of tuberculosis (TB) in humans and bovine TB in mammalian hosts and grows very slowly. Selenium is a central molecule in nitrogen metabolism and an essential ingredient for all living cells and glutamic acid. The effects of selenium on the growth of M. tuberculosis, a representative slow-growing Mycobacterium species, were investigated and measured using the BacT Alert 3D System (MB/BacT System). Sodium selenate, at a final concentration of $10{\mu}g/mL$, reduced the average time-to detection (TTD) to 197.2 hours (95% confidence interval (CI), 179.6~214.8) from 225.1 hours (95% CI, 218~232.0) in the control culture media (P<0.05). The TTD did not increase with $\text\tiny{L}$-glutamate concentrations up to $10{\mu}g/mL$, but a significant reduction in the TTD was observed in the presence of $20{\mu}g/mL$ ${\text\tiny{L}}$-glutamate in culture media (P<0.05). In conclusion, selenate and ${\text\tiny{L}}$-glutamate enhance the growth of M. tuberculosis.

• Journal of Environmental Health Sciences
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• v.24 no.3
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• pp.54-62
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• 1998

20 bacterial strains capable of growing on phenol minimal medium were isolated from soil and wastewater by the enrichment culture technique, and among them, one isolate which was the best in the cell growth was selected and identified as Bacillus sp. SH3 by its characteristics. Strain SH3 could grow with phenol as the sole carbon source up to 15 mM, but did not grow in minimal medium containing above 20 mM of phenol. The optimal conditions of temperature and initial pH for growth and phenol degradation were 30$^{\circ}$C and 7.5, respectively. This strain could grow on various aromatic compounds such as catechol, protocatechuic acid, gentisic acid, o-, m-, p-cresol, benzoic acid, p-hydroxybenzoic acid, anthranilic acid, phenyl acetate and pentachlorophenol, and the growth-limiting log P value of strain SH3 on organic solvents was 3.1. In batch culture, strain SH3 degraded 97% of 10 mM phenol in 48 hours. In continuous culture under the conditions of 20 mM of influent phenol concentration and 0.050 hr$^{-1}$ of dilution rate, the treatment rate of phenol was 94%.

• Journal of environmental and Sanitary engineering
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• v.21 no.1 s.59
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• pp.12-20
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• 2006

This paper was conducted concerning the degree of indoor air quality in public transport vehicles such as taxicabs, buses and subway trains, as sampled through the active participation of drivers and passengers in Seoul between 13th August 2005 and 2nd November 2005. The results were summarized as follows: 1. Among the measured substances especially respirable particulate matters ($PM_{10}$), total bacteria counts (TBC) and carbon dioxide ($CO_2$) exceeded the standard level of $150{\mu}g/m^3,\;800CFU/m^3$ and 1000ppm. 2. The concentration of carbon dioxide ($CO_2$) in taxi recorded 2491ppm, which is more than the standard amount of 1000ppm. This level was comparatively higher than all other public transportation methods. Total bacteria counts (TBC) in bus and subway recorded $1082CFU/m^3\;and\;1856CFU/m^3$, respectively. 3. The drivers who regularly work long hours showed the higher concern about contamination of the air inside the public transport vehicles and they considered it to be worse than the air outside. In contrast, the general public showed less concern about the air quality inside the public transport vehicles. However, they too acknowledged that the quality of the air inside the public transport vehicles was poor. In regards to the degree of indoor air quality in the public transport vehicles, a counterplan must be implemented urgently to effectively combat the excessive levels of $PM_{10}$, microorganism and $CO_2$. We need to gather more conclusive evidence pertaining to other possible contaminants and influencing factors.

• Journal of Dairy Science and Biotechnology
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• v.35 no.2
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• pp.99-104
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• 2017

The global consumption of fructose in the form of high-fructose corn syrup (HFCS) has increased considerably over the past several decades. The current intake of HFCS exceeds that of other major caloric sweeteners such as sucrose. Fructose has potentially adverse effects on human metabolism. Therefore, the objective of this study was to analyze the monosaccharide contents of 13 sweetened milk-based beverages by using high-performance liquid chromatography (HPLC). The total monosaccharide (fructose and glucose) contents of various sweetened milk-based beverages ranged from 0.60 g/100 mL (cheese) to 26.03 g/100 mL (maple caramel snack), while milk showed only 0 g/100 mL monosaccharides. The fructose-to-glucose ratio of various popular sweetened milk-based beverages ranged from not-detected (ND) to 2.24 g/100 mL, but the content of glucose was higher than that of fructose in only three samples, namely, strawberry flavored milk, shrimp chips, and maple caramel snack. The analyzed results revealed important basic information about monosaccharides in various milk-based sweetened beverages, presenting ideas for future fructose research.

• Korean Journal of Adult Nursing
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• v.20 no.1
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• pp.91-101
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• 2008

Purpose: The purpose of this study was to investigate the relationships between a experience of falling, fear of falling, depression, and perceived health status in urban areas. Methods: After obtaining Institutional Review Board (IRB) approval, a one-time, face-to-face, and private interview was conducted with each participant who was eligible and agreed to participate in this study from May 2007 to August 2007 by trained graduate-level nursing students. The questionnaires consisted of Fall Efficacy Scale, K-GDS, and SF-36. The collected data were analyzed with SPSS/PC 12.0 program, which was used for frequency, percentage, mean, standard deviation, t-test, and hierarchical regression. Results: The major findings of this study were as follows; 1) approximately 9% of participants had fallen within one year. Study participants reported moderate fear of falling(M=43.80); moderate physical health (M=42.31) and mental health(M=46.05); and low depression status(M=10.38). 2) there were significant differences in fear of falling according to gender, experience of falling, exercise, and depression. 3) significant factors influencing on fear of falling were experience of falling and physical health status. Conclusion: The results of this study indicate that nurses working closely with older adults should be taught the impact of previous falls and physical health status on fear of falling and need to reinforce exercise behavior for older adults who are vulnerable to fear of falling.

• Journal of radiological science and technology
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• v.44 no.5
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• pp.495-502
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• 2021

This study aims to provide basic data for elderly health insurance policy and medical radiation safety management by analyzing the general radiography usage and exposure dose of the elderly in Korea. The effective dose for each general radiography was calculated using the ALARA-GR program for 260 general radiography codes selected from 'National Health Insurance Care Benefit Cost'. The usage of general radiography was analyzed in the 2016 elderly patient data of the Health Insurance Review and Assessment Service, and the effective dose for each general radiography was applied. The general radiography usage and exposure dose per person aged 65 years and over was 6.47 cases and 0.56 mSv. Females showed higher value than males as 7.15 cases and 0.66 mSv(p<.001). By age, those between 75 and 79 showed the highest number as 6.97 cases and 0.62 mSv(p<.001). Those who were supported by Medical Aid showed higher value than those who were insured by National Health Insurance as 8.82 cases and 0.76 mSv(p<.001). In addition, the ratio by radiography was in the order of Chest 20.85%, Knee Joint 15.58%, and L-spine 14.67%, and the exposure dose was L-spine 29.40%, Chest 15.82%, Abdomen 7.97%, and Entire Spine 7.20%. General radiography, which is widely used due to the high frequency of diseases in the elderly population should be taken into consideration when establishing health insurance policies. In addition, it is necessary to check whether the general radiography with high exposure dose is performed as a routine examination without considering medical necessity.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.2
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• pp.231-241
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• 1998

To assess biological indoor air quality in hospital, concentrations of viable airborne microbes were determined at intensive care unit(ICU), patient room (PR), outpatient waiting room(OPWR) in hospitals of large(1000 beds), middle(500 beds), small(100 beds) hospitals, respectively. Gram positive bacteria, gram negative bacteria, fungi were sampled using suctional sampling method by RCS sampler (Reuter centrifugal air sampler) and RCS GK-A agar plate. In gram positive bacteria groups, CNS(Coagulase Negative Staphylococcus), Micrococcus, Lactobacillus, S. aureus, Enterococcus, St. viridans identified. In gram negative bacteria groups, A. baumannii, Kl. peumoniae and E. coli were identified, and Penicillium was identified in fugi groups. Results of the study were as follows. 1. The highest concentrations of airborne microbes was $971CFU/m^3$ at 5:00 PM in small hospital patient room, and average concentrations of airborne microbes in large, middle and small hospitals were $282CFU/m^3$, $289CFU/m^3$ and $625CFU/m^3$, respectively. Average concentrations of airborne microbes in office(control) was $90CFU/m^3$. Thus, the small hospital showed the worst condition. 2. Representatives of 8 different genera were identified in 150 samples. The most frequently isolated organisms were Staphylococcus (73.0%), Micrococcus (20.7%) and Lactobacillus (4.7%), respectively. Pathogenic microbes isolated were A. baumannii, E. coli, Enterococcus, Kl. peumoniae, S. aureus, St. viridans and Penicillium as fungi. In office, no pathogenic microbes were identified. Average concentrations of airborne pathogenic microbes in large, middle and small hospital were $5CFU/m^3$ (2%), $11CFU/m^3$ (4%) and $12CFU/m^3$ (2%), respectively. Thus, condition in a large hospital was better than those in a middle and a small hospital.

• Parasites, Hosts and Diseases
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• v.56 no.2
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• pp.147-152
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• 2018

This prospective study was aimed to detect acute and chronic ocular toxoplasmosis by comparison of anti-Toxoplasma gondii IgM and IgG antibody levels and IgG avidity test. One hundred and seventeen patients with ocular toxoplasmosis (OT) who referred to the Farabi Eye Hospital, Tehran, Iran were included in this study. Of the patients, 77 cases were positive for anti-T. gondii IgG, and 8 cases were positive for anti-T. gondii IgM. IgG avidity test revealed 11, 4, and 102 cases were low, intermediate, and high, respectively, and 6.8% and 9.4% of cases were positive for IgM and IgG avidity tests, respectively (P=0.632). Agreement (Kappa value) between paired tests IgG-IgM, IgG-IgG avidity, and IgM-IgG avidity was 0.080, 0.099, and 0.721, respectively (P<0.05). This study showed that conventional serologic tests (IgM and IgG levels) and IgG avidity correlate well each other and can be used to differentiate recent infections from old OT. It seems that reactivated old infections rather than recently acquired infections are majority of Iranian OT patients.