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Inhibitory Effects of Tributyltin Acetate on Dopamine Biosynthesis in PC12 Cells  

Kim Yu-Mi (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
Lee Jae-Joon (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
Lee Myung-Koo (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
YAKHAK HOEJI / v.50, no.2, 2006 , pp. 105-110 More about this Journal
Abstract
The effects of tributyltin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with TBTA at $0.05\sim0.25{\mu}M$ significantly decreased the intracellular dopamine content in a concentration-dependent manner ($IC_{50}$ value, $0.17{\mu}M$). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by $0.1{\mu}M$ TBTA at 24 h, and recovered there-after. In addition, treatment with L-DOPA at 20 and $50 {\mu}M$ increased the intracellular dopamine content in PC12 cells and the increase in dopamine content by L-DOPA was significantly abolished by TBTA at $0.1\sim0.2{\mu}M$. These results indicate that TBTA at $0.1\sim0.2{\mu}M$ causes the decrease in the basal dopamine content and abolishes the increase in dopamine content in L-DOPA-treated cells in part by the inhibition of TH gene expression and activity.
Keywords
tributyltin acetate; dopamine content; tyrosine hydroxylase; PC12 cells;
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