YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effects of Tributyltin Acetate on Dopamine Biosynthesis in PC12 Cells

Tributyltin 화합물이 PC12 세포의 Dopamine 생합성 저해작용에 미치는 영향

  • Kim Yu-Mi (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Jae-Joon (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee Myung-Koo (College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2006.04.01
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Abstract

The effects of tributyltin acetate (TBTA), one of the endocrine-disrupting organotin compounds, on dopamine biosynthesis in PC12 cells were investigated. Treatment of PC12 cells with TBTA at $0.05\sim0.25{\mu}M$ significantly decreased the intracellular dopamine content in a concentration-dependent manner ($IC_{50}$ value, $0.17{\mu}M$). Under these conditions, tyrosine hydroxylase (TH) activity and TH mRNA level were also decreased by $0.1{\mu}M$ TBTA at 24 h, and recovered there-after. In addition, treatment with L-DOPA at 20 and $50 {\mu}M$ increased the intracellular dopamine content in PC12 cells and the increase in dopamine content by L-DOPA was significantly abolished by TBTA at $0.1\sim0.2{\mu}M$. These results indicate that TBTA at $0.1\sim0.2{\mu}M$ causes the decrease in the basal dopamine content and abolishes the increase in dopamine content in L-DOPA-treated cells in part by the inhibition of TH gene expression and activity.

Keywords

References

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