• 대한화학회지
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• 제45권3호
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• pp.223-229
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• 2001

흐름주입 장치를 이용하여 화학발광법에 의한 전혈 중의 당을 정량하는 방법에 대하여 연구하였다. 당의 효소반응에서 생성되는 과산화수소에 의하여 424nm에서 발생하는 luminol의 화학발광 세기의 차이를 정량에 이용하였다. 효소반응기는 glucose oxidase를 aminopropyl glass bead에 혼입하여 만들었으며, 흐름셀에서 발생하는 화학발광의 세기는 광섬유 다발을 이용하여 측정하였다. 최적 실험조건을 구하기 위하여 화학발광 시약 및 효소반응기의 pH, 흐름속도 및 온도가 화학발광세기에 미치는 영향을 조사하였다. 최적실험조건에서 구한 검정 곡선은 $1.0{\times}10^{-1}$~ 7.0mM에서 직선성이 성립하였으며, 검출한계는 $6.0{\times}10^{-2}$mM이었다. 본 방법을 전혈 중의 당 정량에 적용하였으며, 그 결과를 기존의 분석법에서 구한 결과와 비교하였다. 또한, 회수율 측정을 통하여 본 방법의 신뢰성을 검증하였다.

• 한국토양비료학회지
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• 제35권6호
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• pp.327-334
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• 2002

우리나라 유통상토에 대한 물리적 표준분석법을 설정하기 위한 연구를 실시하였다. 국내 시중에 유통되고 있는 53개 품목의 원예용 상토와 9개 품목의 수도용 상토를 대상으로 설정된 분석법을 적용하여 실험하였다. 입자밀도의 측정은 가스형 피크노메타 (Micromeritics Co. Model 1305)를 사용하면 속도와 정확성 면에서 매우 유리 하였다. 용적밀도는 구미에서 Sandbox법에 의한 용적밀도를 기본으로 하기 때문에 이와 유사한 성적을 얻을 수 있는 다양한 실험을 한 결과, 추다짐법 (Plunger compaction method)이 가장 유의성 있는 측정 방법으로 밝혀졌다. 한편 가스형 피크노메타법에 의한 우리나라 유통상토의 입자밀도는 원예용이 1.93, 수도용이 $2.43Mg\;m^{-3}$로 나타났으며, 추다짐법에 의한 용적밀도는 원예용이 0.22, 수도용이 $1.01Mg\;m^{-3}$로 각각 나타났다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -1
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• pp.490-493
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• 2002

In this paper, the old Fourier-Motzkin method (abbreviated as the old FH method from now on) is first modified to the form which can derive all minimal vectors as well as all minimal support vectors of nonnegative integer homogeneous solutions (i.e., T-invariants) for a matrix equation $Ax=b=0^{m{\times}1}$, $A\epsilonZ^{m{\times}n}$ and $b\epsilonZ^{m{\times}1}$, of a given Petri net, where the old FM method is a well-known and direct method that can obtain at least all minimal support solutions for $Ax=0^{m{\times}1}$ from the incidence matrix . $A\epsilonZ^{m{\times}n}$, Secondly, for $Ax=b\ne0^{m{\times}n}$ a new extended FM method is given; i.e., all nonnegative integer minimal vectors which contain all minimal support vectors of not only homogeneous but also inhomogeneous solutions are systematically obtained by applying the above modified FH method to the augmented incidence matrix $\tilde{A}$ =〔A,-b〕$\epsilon$ $Z^{m{\times}(n＋1)}$ s.t. $\tilde{A}\tilde{x}$ = 0^{m{\times}1}$ However, note that for this extended FM method we need some criteria to obtain a minimal vector as well as a minimal support vector from both of nonnegative integer homogeneous and inhomogeneous solutions for Ax=b. Then those criteria are also discussed and given in this paper.

• Journal of Ginseng Research
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• 제9권2호
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• pp.154-162
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• 1985

We report a newassay method on the measurement of the catalase activity, whose utilzation value is considered to be remarkable in the field of plant biochemistry. We named this method as a De-Coupling method. The essence of de-coupling method is the separation between the enzyme reaction and the indicator reaction. The optimum condition of the enzyme reaction was found to be following: on addition of 1 ml of substrate (H2O2: 20mM) to the fixture of the crude extract of enzyme (volume: 0.2 ml) and the ammonium phosphate buffer (volume: 1.8 ml; 0.93 M phosphate, 1.6M NHB, 2.5 M methanol, pH 7.0). After 30, 60 and 90 seconds of the enzyme reactions are proceeded, the reactions are terminated by 25% of tai-chloro-acetate (final concentration of 5%), respectively. The precipitated materials by tai-chloro-acetate was removed by the centrifugation (2000g, 10minutes). Formaldehyde produced in the enzymatic reaction was reacted with 2ml of acetylacetone (60mM). The indicator reaction -(HANTSCH REAKT10N)- in which lutidine is formed, was proceeded for 60 minutes at $25^{\circ}C$.

• 충청수학회지
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• 제23권4호
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• pp.845-852
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• 2010

A nonlinear algebraic equation f(x) = 0 is considered to find a root with integer multiplicity $m{\geq}1$. A variant of Newton-secant method for a multiple root is proposed below: for n = 0, 1, $2{\cdots}$ $$x_{n+1}=x_n-\frac{f(x_n)^2}{f^{\prime}(x_n)\{f(x_n)-{\lambda}f(x_n-\frac{f(x_n)}{f^{\prime}(x_n)})\}$$, $$\lambda=\{_{1,\;if\;m=1.}^{(\frac{m}{m-1})^{m-1},\;if\;m{\geq}2$$ It is shown that the method has third-order convergence and its asymptotic error constant is expressed in terms of m. Numerical examples successfully verified the proposed scheme with high-precision Mathematica programming.

• 핵의학기술
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• 제27권1호
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• pp.42-46
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• 2023

Purpose The reference range described in Adrenocorticotropic Hormone reagent used in our laboratory is 10-60 pg/mL at 8 a.m. to 10 a.m., and 6-30 pg/mL at 8 p.m. to 10 p.m. However, in the case of outpatients, blood is mainly collected between 10 a.m. and 6 p.m., accounting for 57.8% of the total. Therefore, This study is intended to help make a more accurate diagnosis by reevaluating the reference range provided by the manufacturer of the Adrenocorticotropic Hormone reagent and setting split-timed reference range. Materials and Methods The patients collected blood before 10 a.m. were group A (68 people), and the patients collected blood after 10 a.m. were set to group B (80 people). A T-test was performed between groups to test their significance. And it was confirmed whether it was necessary to set the gender classification as a subgroup. The method of setting the reference range was calculated by the Bayesian's method and the Hoffmann's method. Results The reference range of Group A was 8.6 to 60.6 pg/mL by the Bayesian's method, and the Hoffmann's method was 3.6 to 61.3 pg/mL. The reference range of Group B was 6.9 to 50.5 pg/mL when applying the Bayesian's method, and the Hoffmann method's was 2.3 to 48.9 pg/mL. Conclusion This study was concluded that it was necessary to set the split-timed reference range. Through this study, the later the blood collection time, the lower the level of Adrenocorticotropic Hormone, indicating that blood collection time is important for patients with clinical significance. If a large number of subjects are selected and supplemented in the future, it is believed that systematic and accurate reference range can be set.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2003년도 ISIS 2003
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• pp.579-582
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• 2003

In this paper, we propose a 4-step inference method needed for constructing a natural language communication system. The method is used to obtain fuzzy quantifier Q′when QA is Fisr τ⇔ Q′(m′A) is mF is m"is τ is inferred (Q, Q′: quantifiers, A: fuzzy subject, m′, m": modifiers, y: fuzzy predicate, τ: truth qualifier). We show that Q′is resolved step by step for two types of Q, including a non-increasing type (few,...) and a non-decreasing type(most,...).

• Journal of information and communication convergence engineering
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• 제8권3호
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• pp.317-322
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• 2010

This paper presents an algorithm generating inverse element over finite fields GF($3^m$), and constructing method of inverse element generator based on inverse element generating algorithm. An inverse computing method of an element over GF($3^m$) which corresponds to a polynomial over GF($3^m$) with order less than equal to m-1. Here, the computation is based on multiplication, square and cube method derived from the mathematics properties over finite fields.

• 정보보호학회논문지
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• 제13권2호
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• pp.127-132
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• 2003

Cryptosystems have received very much attention in recent years as importance of information security is increased. Most of Cryptosystems are defined over finite or Galois fields GF($2^m$) . In particular, the finite field GF($2^m$) is mainly used in public-key cryptosystems. These cryptosystems are constructed over finite field arithmetics, such as addition, subtraction, multiplication, and multiplicative inversion defined over GF($2^m$) . Hence, to implement these cryptosystems efficiently, it is important to carry out these operations defined over GF($2^m$) fast. Among these operations, since multiplicative inversion is much more time-consuming than other operations, it has become the object of lots of investigation. Recently, many methods for computing multiplicative inverses at hi호 speed has been proposed. These methods are based on format's theorem, and reduce the number of required multiplication using normal bases over GF($2^m$) . The method proposed by Itoh and Tsujii[2] among these methods reduced the required number of times of multiplication to O( log m) Also, some methods which improved the Itoh and Tsujii's method were proposed, but these methods have some problems such as complicated decomposition processes. In practical applications, m is frequently selected as a power of 2. In this parer, we propose a fast method for computing multiplicative inverses in GF($2^m$) , where m = ($2^n$) . Our method requires fewer ultiplications than the Itoh and Tsujii's method, and the decomposition process is simpler than other proposed methods.

• 분석과학
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• 제36권4호
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• pp.180-190
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• 2023

The presence of process related impurities in any drug or the drug product was associated with its safety, stability and efficacy. The overall literature survey proved that there is no method published on the assessment of process related impurities in brigatinib. In this study, a simple, reliable and stable HPLC qualitative method was reported for quantification of process related impurities with easy and quick extraction procedure. The impurities along with standard brigatinib was resolved on Lichrospher^® C18 (250 mm × 4.6 mm; 5 ㎛ particle size) column in room temperature using methanol, acetonitrile, pH 4.5 phosphate buffer in 55:25:20 (v/v) at 1.0 mL/min as mobile phase and UV detection at 261 nm. The method produces well resolved peaks at retention time of 4.60 min, 12.28 min, 3.37 min, 7.34 min and 8.39 min respectively for brigatinib, impurity A, B, C and D. The method produces a very sensitive detection limit of 0.0065 ㎍/mL, 0.0068 ㎍/mL, 0.0053 ㎍/mL and 0.0058 ㎍/mL for impurity A, B, C and D respectively with calibration curve linear in the concentration range of 22.5-135 ㎍/mL for brigatinib and 0.0225-0.135 ㎍/mL for impurities. The method produces all the validation parameters under the acceptable level and doesn't produces any considerable changes in peak area response while minor changes in the developed method conditions. The method can effectively resolve the unknown stress degradation products along with known impurities with less % degradation. The method can efficiently resolve and quantify the impurities in formulation and hence can suitable for the routine quality analysis of brigatinib in raw material and formulation.