• 동의생리병리학회지
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• 제17권1호
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• pp.91-100
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• 2003

In the present study, we investigated the protective effect of the Lycii Fructus water extracts (LFE) against CCl4-induced hepatotoxicity and the mechanism underlying these protective effects in the rats. The pretreatment of LFE has shown to possess a significant protective effect by lowering the serum alanine and aspartate aminoteansferase (AST and ALT) and alkaline phosphatase (ALP). This hepatoprotective action was confirmed by histological observation, In addition, the pretreatment of LFE prevented the elevation of hepatic malondialdehyde (MDA) formation and the depletion of reduced glutathione (GSH) content and catalase activity in the liver of CC1₄-injected rats. The LFE also displayed hydroxide radical scavenging activity in a dose-dependent manner (IC50 = 83.6 μg/ml), as assayed by electron spin resonance (ESR) spin-trapping technique. Moreover, the expression of cytochrome P450 2E1 (CYP2E1) mRNA, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR), was significantly decreased in the liver of LFE-pretreated rats when compared with that in the liver of control group. Based on these results, it was suggested that the hepatoprotective effects of the LFE may be related to antioxidant effects and regulation of CYP2E1 gene expression.

• 대한한의학방제학회지
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• 제20권1호
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• pp.51-65
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• 2012

Objectives : In this study the antidepressant effects of mixture of Lycii Radicis Cortex and Gardeniae Fructus on the change of HPA-Axis and Catecholamine system was investigated Methods : The forced swimming test was performed. The expression of CRF, c-Fos in the PVN, and TH in the VTA and LC were measured with immunohistochemical method. And the experimental groups were divided into the Extraction after Mix group(A) and Mix after Extraction group(B). The effects of both group were compared. Results : The duration of immobility in the FST was decreased significantly in three groups except B100(P<0.01). B100 was shown the significant increase(P<0.05). The expression of CRF in PVN was decreased significantly in all group(P<0.05~P<0.01). The expression of c-Fos in PVN was decreased in A100, A400 and was increased significantly in B100, B400(P<0.05). The expression of TH in VTA and LC were decreased significantly in all group(P<0.001). Conclusions : Effects of Extraction after Mix group(A) and Mix after Extraction group(B) on the HPA-Axis system and Catecholamine system were validated.

• 한국생활과학회지
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• 제23권6호
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• pp.1217-1229
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• 2014

The purpose of this study was to investigate the quality characteristics and antioxidative effect of Dasik prepared with Lycii fructus extract(LD). LD were added in ratios (w/w) of 0(C), 5(LD1), 10(LD2), and 15%(LD3), and then proximate compositions, physicochemical properties, sensory evaluations and antioxidative effect of the Dasik were measured. LD1~LD3 samples showed higher contents of moisture, crude lipid, crude protein and crude ash as well as pH and sugar content compared to control (p<0.001). The total phenol content of LD samples (LD1, 31.5% ; LD2, 66.1% ; and LD3, 69.7%) was higher than that of control (4.71%). DPPH radical scavenging activity of LD3 showed the highest activity of 65.3%, whereas that of control (27.0%) was the lowest among samples. Additionally, ABTS radical scavenging activity of LD samples was higher than that of control (C, 38.9% ; LD1, 53.6%; LD2, 80.5% ; and LD3, 83.3%). The results of texture analysis on all samples showed that control had the highest but LD3 was the lowest (p<0.001). In sensory evaluation, the scores of appearance, flavor, taste and overall preference for LD2 was significantly higher than the samples(C, LD1, and LD2). Form the findings, this study suggests that 10% addition of LD was effective for preparation of Dasik in the aspects of the consumer acceptability and functionality.

• 약학회지
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• 제51권5호
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• pp.296-300
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• 2007

The purpose of this study was to investigate the preventive effects of Lycii fructus Extract (LFE) against the acute hepatotoxicity-inducing lipopolysaccharide (LPS) in the liver. LFE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. On the day 21, 1.5 ml/kg of LPS dissolved in saline was injected 4 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), lactate dehydrogenase (LDH) in serum of rats, superoxide dismutase (SOD) in mitochondrial fraction, and malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx) in liver homogenate. LPS-treatment markedly increased the levels of GOT, GPT, LDH and MDA, and significantly decreased those of SOD, CAT and GPx. But LFE-pretreatment decreased the levels of GOT, GPT, LDH and MDA, by 17.7%, 27.5%, 40.7% and 56.9%, respectively and increased those of SOD, CAT and GPx, by 90.5%, 78.9% and 83.8%, respectively. These results showed that the LFE had the preventive effects against the acute hepatotoxicity-inducing LPS in the liver.

• 한국식품조리과학회지
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• 제21권1호
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• pp.94-102
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• 2005

The purpose of this study was to investigate the antioxidative effect and quality characteristics of cookies made with Lycii fructus powder(LFP). The cookies were stored at $50^{\circ}C$ for 40 days. The LFP additive increased the moisture, ash, crude protein and volume of the cookies. When LFP was added at $5\%$ into the cookies, the spread ratio was higher than for the control, the $10\%$ and the $20\%$ LFP cookies. As more LFP was added, the L-value decreased, and the a--values and b-values increased for the color values. For the textural characteristics, springiness and cohesiveness of the control cookies were the highest among the samples, the other side, brittleness and gumminess were higher in cookies made with LFP than in the control cookies. Overall, the cookies made with $5\%$ LFP were preferred more than the other cookies, as tested forby sensory evaluation. The cookies made with LFP contained mostly palmitic acid (C16:0) followed by oleic acid (C18: 1) and stearic acid (C18:0). The acid value, peroxide value, and thiobarbituric acid value were lower in cookies made with $5\%$ and $10\%$ LFP than in those cookies made with $20\%$ LFP and the control cookies.

• 한국식품조리과학회지
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• 제24권4호
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• pp.480-486
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• 2008

구기자의 수용성 추출물을 첨가한 시럽에 집청한 유과(YU-G)를 제조하여 PE 필름 봉지에 넣고 함기 포장한 후 상온에서 12주간 저장하면서 유과의 품질 특성을 검토하였다. 저장하는 동안 YU-G는 구기자 추출물을 첨가하지 않은 시럽에 집청한 유과(YU-NG)에 비해 수분함량은 높았고 기계적 경도는 낮았다. 저장시 YU-NG와 YU-G의 L값은 감소하고 a값과 b값은 증가하였다. 상온 저장 시 유과의 과산화물가와 산가가 증가하였으며, YU-G는 YUNG에 비해 과산화물가와 산가가 낮았다. 12주 저장 후 YU-G 의 과산화물가는 식품공전 기준치인 40 meq/kg 보다 낮았고 산가는 식품공전기준치인 4 KOH mg/g 보다 낮은 값을 보였으나, YU-NG의 과산화물가는 6주 저장 후, 산가는 10주 저장 후 식품공전 기준치를 초과하였다. 유과의 관능검사 결과, YU-G는 YU-NG보다 저장하는 동안 전반적인 선호도가 높았다.

• Korean Journal of Acupuncture
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• 제36권4호
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• pp.221-229
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• 2019

Objectives : This study investigated the hepatoprotective effect of herbal mixture including Lycii fructus (HML) in thioacetamide (TAA)-induced hepatotoxicity in mice. Methods : To confirm the liver protective effect, induced by TAA for 3 days injection at 100 mg/kg mice, HML were treated for 8 weeks at 300 mg/kg/day, 1000 mg/kg/day. Positive control was treated silymarin 50 mg/kg/day after TAA injection. The changes of mortality rate, clinical signs, organ weight, relative liver, blood chemistry and histopathological findings were analyzed after experiment. Results : Body weight gain was observed in all groups, but TAA treated group at 4th week and all treated groups decreased weight compared to the untreated group. As a result of organ weight measurement, organ weight gain due to hepatic injury was observed statistically significantly in TAA-treated group and TAA+Silymarin treated group, and the herbal mixture-treated group showed a tendency to decrease compared to the TAA treated group. Blood biochemistry showed that total cholesterol and very low density lipoprotein cholesterol decreased statistically in TAA+low-dose and high dose herbal mixture treated group compared to the TAA-treated group. Histopathological examination showed that liver abnormalities were not observed in untreated group, liver fibrosis was observed in liver injury with TAA treated and herbal mixture treated group. And, TAA+high dose herbal mixture group showed relaxation tendency on liver calcification compared to the TAA treated group. Conclusions : According to the above results, HML provided hepatoprotective effects on the hepatic injury by reduction of inflammatory responses.

• 한국식품과학회지
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• 제27권5호
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• pp.768-772
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• 1995

건 구기자와 볶음조건을 달리한 볶은 구기자의 열수 추출물에 대하여 여러 가지 이화학적 특성을 조사하였다. 건 구기자의 일반성분은 수분 17.5%, 회분 5.4%, 지방 14.7%, 단백질 18.9%, 섬유질 12.8%, 당질 31.7%였다. 열수 추출시 추출수율은 볶음온도와 시간이 증가할수록 가용성 고형분이 더 빨리 추출되었으며, 색도는 명도가 감소하고 적색도가 증가하여 추출물이 더 진한 갈색을 나타내었고 탁도 역시 증가하였다. pH는 볶음온도와 시간이 증가할수록 5.08에서 4.38로 감소하였고 산도는 0.09%에서 0.16%로 증가하였다. 유리당 함량은 볶음온도와 시간이 증가할수록 감소하였고 특히 glucose함량이 0.45%에서 0.12%로 크게 감소하였다. 지방산 조성은 건 구기자에 비해 볶음온도와 시간이 증가할수록 linoleic acid의 비율이 감소하고 palmitic acid의 비율이 증가하였다. 관능검사 결과는 볶음온도와 시간이 증가할수록 단 냄새, 탄 냄새, 신 맛, 탄 맛, 떫은 맛 등은 증가하였고 단 맛과 구수한 맛은 약간 감소하였다.

• 대한영양사협회학술지
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• 제18권3호
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• pp.213-221
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• 2012

The principal objective of this study was to evaluate the quality characteristics of mungbeanmook prepared with five different levels (0, 5, 10, 15, and 20%) of Gugija (Lycii fructus) infusion. We analyzed Hunter's color values, pH, texture characteristics, and perfomed sensory evaluation of mungbeanmook prepared with Gugija infusion. The pH levels of mungbeanmook ranged between 5.45 and 6.12 and did not show significant differences. As Gugija infusion concentration increased, the color lightness "L" value decreased, whereas redness color "a" value and yellowness color "b" values increased (P<0.05). With regard to mechanical texture properties of the mungbeanmook samples, as the Gugija infusion concentration increased, the scores of hardness, chewiness, and gumminess all significantly decreased (P<0.05) whereas there were no significant differences in springiness or cohesiveness. The results of the sensory evaluation showed that the score of the LFLT10 sample (10% Gugija infusion) was significantly higher than those of others in terms of taste, color, flavor, texture, and overall quality (P<0.05). Taken together, the recommended optimum level of Gugija infusion in mungbeanmook is 10% for sensory evaluation. Gugija infusion can be a useful approach to improving quality of mungbeenmook as a functional food.

• 한방안이비인후피부과학회지
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• 제33권4호
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• pp.44-54
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• 2020

Objectives : In the present study, we investigated whether or not an herb mixture including Lycii fructus(HML) has attenuation effects on light stress in rats. Methods : Light stress was induced by exposure to 300 lx of constant lifht for 24 h and 1mg/kg of caffeine was injected into the rat. Rats were orally treated with distilled water of HML for 21 days at 100mg/kg/d. The changes of mortality rate, clinical signs, body, blood chemistry, melatonin and IL-2 expression were analyzed after experiment. Results : Normal weight changes were observed in all treatment groups. In addition, no changes were observed in dead animals and general symptoms. At the time of autopsy, the appearance of all groups and gross examination of major organs were observed, and no gross findings were observed. Blood biochemical tests showed that total cholesterol and HDL cholesterol levels were statistically significantly decreased in the group treated with HML compared to the stress-induced group. Melatonin was measured in the blood, but there was no significant difference in the group treated with HML compared to the group with stress-induced group, but it tended to increase. IL-2 was measured in the blood, no significant change was observed in the group treated with HML compared to the stress-induced group. Conclusions : According to the above results, HML maybe help with hematological changes expressed by caffeine and light-induced stress.