Browse > Article

The Preventive Effects of Lycii fructus Extract Against LPS-induced Acute Hepatotoxicity  

Kang, Kum-Suk (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kwon, Ryun-Hee (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kim, In-Deok (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
YAKHAK HOEJI / v.51, no.5, 2007 , pp. 296-300 More about this Journal
Abstract
The purpose of this study was to investigate the preventive effects of Lycii fructus Extract (LFE) against the acute hepatotoxicity-inducing lipopolysaccharide (LPS) in the liver. LFE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5 ml/kg for 20 days. On the day 21, 1.5 ml/kg of LPS dissolved in saline was injected 4 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), lactate dehydrogenase (LDH) in serum of rats, superoxide dismutase (SOD) in mitochondrial fraction, and malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx) in liver homogenate. LPS-treatment markedly increased the levels of GOT, GPT, LDH and MDA, and significantly decreased those of SOD, CAT and GPx. But LFE-pretreatment decreased the levels of GOT, GPT, LDH and MDA, by 17.7%, 27.5%, 40.7% and 56.9%, respectively and increased those of SOD, CAT and GPx, by 90.5%, 78.9% and 83.8%, respectively. These results showed that the LFE had the preventive effects against the acute hepatotoxicity-inducing LPS in the liver.
Keywords
Lycii fructus; LPS; SOD; hepatotoxicity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Jian, Y. : Modern Study of Chinese Drugs, Clinical Applications. Ancient Book Press of Chinese Medicine, China (1997)
2 Finkelstein, J. D., Kyle, W. E. and Harris, B. J. : Methionine metabolism in mammals. Regulation of homocysteine methyl-transferases in rat tissue. Arch. Biochem. Biophys. 146, 84 (1971)
3 Kim, H. S., Park, Y. S. and Kim, C. I.: Changes of serum lipid profiles after eating Lycii Fructus in rats fed high fat diet. Korean Nutr. Soc. 31, 263 (1998)
4 Kinght, J. A. : The process and theories of aging. Ann. Clin. Lab. Sci. 25, 2289 (1995)
5 Lowry, O. H., Rosenbrough, N. J., Farr, A. S. and Randall, R. J.: Protein Measurenment with Folin phenol reagent. J. Biol. Chem. 193, 256 (1951)
6 Lawrence, R. A. and Burk, R. E : Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71, 952 (1976)
7 Gabriel, L. R and William, R. H. : Principles and Methods of Toxicology. Raben Press, p. 407 (1982)
8 Rosen, D. R., Jakobisiak, M., Hartmann-Petersen, R., Ortega, S. and Johnston, D. J. : Mutations with familial amyotrophic lateral sclerosis. Nature 362, 59 (1993)
9 Gutteridge, J. M. C, Beard, A. P. C. and Quinlan, G. J. : Superoxide-dependent lipid peroxidation. Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun. 117, 901 (1983)
10 Von, Sonntag. : In The Chemical Basis of Radiation of biology, Tylor and Francis. (ed.). London, p. 31 (1987)
11 Yosjikawa, T., Murakami, M., Yoshida, N., Seto, O. and Kondo, M. : Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haemostas. 50, 869 (1983)
12 Aebi, H. : Catalase in vitro, methods. Enzymology 105, 121 (1984)
13 Kim, N. J., Youn, H. G. and Hong, N. D. : Pharmacological effects of Lycium chinensis. Korean Soc. Pharmacog. 25, 264 (1994)
14 Kang, K. I., Jung, J. Y., Koh, K. H. and Lee, C. H. : Hepetoprotective effects of Lycium chinense Mill fruit extracts and fresh fruit juice. Korean J. Food Sci.Technol. 38, 99 (2006)   과학기술학회마을
15 조명행 : 기초 독성학, 개정판, 영지문화사, 서울 p. 116 (2004)
16 Charles, A. J. : Immunobiology, 5th, Life Science Publication Co, Seoul p. 76 (2002)
17 Park, K. M., Hwang, J. K., Shin, K. M., Kim, H. S. and Song, J. H.: Detoxicating effects of oriental herb extract mixtures on nicotine and dioxin. Korean J. Food Sci. Technol. 35, 980 (2003)
18 Takaharu, N. and Kiyonori, Y. : Low-dose $\gamma$-ray irradiation reduces oxidative damage induced by $CCl_4$ in mouse liver. Free Radical Biology & Medicine 27, 1324 (1999)
19 Yubin, J. : Pharmacological Action : Application of available composition of traditional Chinese medicine. Heilongjiang Science and Technology Press, Heilongjiang (1995)
20 Ha, B. J., Lee, S. H., Kim, H. J. and Lee, J. Y. : The role of salicornia herbacea in ovariectomy-induced oxidative stress. Biol. Pharm. Bull. 29, 1306 (2006)
21 McPhalen, C. A., Vincent, M. G. and Jansonius, J. N. : X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotransferase. J. Mol. Biol. 225, 495 (1992)
22 김희선, 박영숙, 김창임 : 구기자 섭취에 의한 고지방식이를 하는 흰쥐의 혈중 지질상태 변화. 한국영양학회지 31, 263 (1998)
23 Yoon, C. K., Kim, H. H., Chae, S. N., Oh, M. J. and Lee, G. H.: Hepatic oxygen free radical and alcohol metabolizing enzyme activities in rats fed diets supplementes with Lycium chinense ethanol extract. J. Korean Soc. Food Sci. Nutr. 30, 668 (2001)
24 Fridovich, I.: Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm. 247, 1 (1986)
25 Beauchamp, C. and Fridovich, I. : Superoxide dismutase : improved assays and an assay applicable to acrylamide gel. Anal Biochem. 44, 276 (1971)
26 Cho, J. H., Sin, J. S., Km, E. J., Shin, S. H., Jang, J. Y., Shin, K. S., Kim, Y. B., Kang, J. K. and Hwang, S. Y. : Protective effect of Lycii fructus extract against hepatotoxicity induced by carbon tetrachloride. Korean J. Lab. Ani. Sci. 20, 187 (2004)
27 Kim, K. S., Shim, S. H., Jeong, K. H., Cheong, C. S., Ko, K. H., Park, J. I., Huh, H., Lee, B. J. and Kim, B. K. : Anti-diabetic activity of constituent of Lyii fructus. J. App. Pharmocol. 6, 378 (1998)
28 Lee, B. Y, Kim, E. J., Choi, H. D., Kim, Y. S., Kim, I. H. and Kim, S. S. : Physicochemical properties of Boxthorn (Lycii fructus) hot water extracts by roasting conditions. Korean J. Food Sci. Technol. 27, 768 (1995)
29 Dantzer, R., Bluthe, R. M., Gheusi, G., Cremona, S., Laye, S., Parnet, R and Kelley, K. W. : Molecular basis of sickness, behavior. Annals N. Y. Acad. Sci. 13, 856 (1998)
30 Halliwell, B.: How to charactrize a biological antioxidant. Free Radic. Res. Commun. 9, 598 (1990)
31 Choi, S. I., Lee, Y. M. and Heo, T. R. : Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean J. Biotechnol. Bioeng. 18, 282 (2003)
32 Tainer, J. A., Getzoff, E. D., Richardson, J. S. and Richardson, D. C. : Structure and mechanism of Cu, Zn, superoxide dismutase. Nature 306, 274 (1983)
33 Charles, A. J. : Immunobiology, 5th, Life Science Publication Co, Seoul p. 350 (2002)
34 Kim, H. K., Na, G. M., Ye, S. H. and Han, H. S. : Extraction characteristics and antioxidative activity of Lycium chinense extracts. Korean J. Food Pre. 11, 352 (2004)
35 Jiangsu. : Chinese Medicine Dictionary. Shanghai Science and Technology Press Shanghai, China (1979)