Browse > Article
http://dx.doi.org/10.14406/acu.2019.025

Protective Effect of Herbal Mixture Including Lycii Fructus on Hepatotoxicity Induced by Thioacetamide in Mice  

Kim, Yong-Min (Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University)
Hwang, Dong-Suk (Robin Korean Medical Clinic)
Kwak, Byeong-Mun (Department of Meridian and Acupoint, College of Korean Medicine, Semyung University)
Kim, Ee-Hwa (Department of Meridian and Acupoint, College of Korean Medicine, Semyung University)
Publication Information
Korean Journal of Acupuncture / v.36, no.4, 2019 , pp. 221-229 More about this Journal
Abstract
Objectives : This study investigated the hepatoprotective effect of herbal mixture including Lycii fructus (HML) in thioacetamide (TAA)-induced hepatotoxicity in mice. Methods : To confirm the liver protective effect, induced by TAA for 3 days injection at 100 mg/kg mice, HML were treated for 8 weeks at 300 mg/kg/day, 1000 mg/kg/day. Positive control was treated silymarin 50 mg/kg/day after TAA injection. The changes of mortality rate, clinical signs, organ weight, relative liver, blood chemistry and histopathological findings were analyzed after experiment. Results : Body weight gain was observed in all groups, but TAA treated group at 4th week and all treated groups decreased weight compared to the untreated group. As a result of organ weight measurement, organ weight gain due to hepatic injury was observed statistically significantly in TAA-treated group and TAA+Silymarin treated group, and the herbal mixture-treated group showed a tendency to decrease compared to the TAA treated group. Blood biochemistry showed that total cholesterol and very low density lipoprotein cholesterol decreased statistically in TAA+low-dose and high dose herbal mixture treated group compared to the TAA-treated group. Histopathological examination showed that liver abnormalities were not observed in untreated group, liver fibrosis was observed in liver injury with TAA treated and herbal mixture treated group. And, TAA+high dose herbal mixture group showed relaxation tendency on liver calcification compared to the TAA treated group. Conclusions : According to the above results, HML provided hepatoprotective effects on the hepatic injury by reduction of inflammatory responses.
Keywords
liver injury; hepatitis; thioacetamide; lycii fructus;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Jeong JS, Shin MK. Dictionary of Korean Traditional Medicine, Youngrim-sa, Seoul. : 1990 : 386-88
2 Zhao YZ, Lee SH, Huh JW, Ra JC, Sohn DH. Hepatoprotecive Effects of Alnus japonica Extract on Experimental Liver Injury Models. Yakhak Hoeji. 2012 ; 56(2) : 99-107.
3 Lee TJ, Min KJ. The effect of Allium sativum L. extract on hepatic function in rats with CCl4-induced (hepatic) injury. J Korea Aca Ind Soc. 2010 ; 11(5) : 1936-42. https://doi.org/10.5762/KAIS. 2010.11.5.1936
4 Kim NJ, Youn WG, Hong ND. Pharmological effect of Lycium chinese. Kor J Pharmacogn. 1994 ; 25(3) : 264.
5 Kim BW, Roh KS. Study on the activity of GOT and GPT in the hepatotoxic rat treated Lycium chinese. Kor J Biomed Lab Sci. 2000 ; 6(3) : 187-92.
6 Kim JS, Hong HS. Construction of Model for Health- related Quality of Life of Liver Cirrhosis Patients. J Korean Bio Nur Sci. 2014 ; 16(4) : 292-99. https://doi.org/10.7586/jkbns.2014.16.4.292   DOI
7 Seung HS, Woo HJ. Inhibitory Effect of YBR on Hepatic Fibrogenesis. Korean J Orient Int Med. 2010 ; 31(2) : 314-30.
8 Kim KJ, Shin MR, Kim SH, Kim SJ, Lee AR, O JK, et al. Protective effect of Thongyuhwalhyeol-tang on liver injury in thioacetamide- induced rat. Korean J Herbology. 2018 ; 33(1) : 37-46. https://doi.org/10.6116/kjh.2018.33.1.37
9 Hunter AL, Hosche MA, Neal RA. Thioacetamide-induced hepatic necrosis. Involvement of the mixed-function oxidase enzyme system. J Pharmacol Exp. Ther. 1977 ; 200(2) : 439-48.
10 Fitzhugh OG, Nelson AA. Liver tumors in rats fed thiourea or thioacetamide. Science. 1948 ; 108(2014) : 626-28.   DOI
11 Robenek H, Themunn H. Effects of chronic administration of thioacetamide on the structure of bile canaliculi and tight junctions in the rat liver as reealed by freeze-fracturing. Virchows Arch. 1979 ; 321(1) : 57-61.
12 Satyabhama S, Padmanaban G. Effects of thioacetamide on cytochrome P450 synthesis in rat liver. Biochem J. 1984 ; 218(2) : 371-77.   DOI
13 Nikolaev V, Kermova M, Naydenova E, Ivanov E, Adjarov D. Biochemical Changes in the rat after chonic thiacetamide intoxication. Toxicology. 1998 ; 48(1) : 81-5.   DOI
14 Moller A, Dargel R. Structural and functional impairment of mitochondria from rat livers chronically injured by thioacetamide. Acta Pharmacol Toxicol. 1984 ; 55(2) : 126-32.   DOI
15 Landon EJ, Naukam RJ, Sastry BVR. Effects of calcium channel blocking agents on calcium and centrilobular necrosis in the liver of rats treated with hepatotoxic agents. Biochem Pharmacol. 1986 ; 35(4) : 597-600.
16 Gallagher CH, Gupta DN, Judah JD, Rees KR. Biochemical changes in liver in acute thioacetamide intoxication. J Pathol. 1956 ; 72(1) : 193-96.   DOI
17 Dwivedi Y, Rastogi R, Sharma SK, Gang NK, Dhawan BN. Picroliv affords protection against thioacetamide-induced hepatic damage in rats. Planta Med. 1991 ; 57(1) : 25-28.   DOI
18 Yoon CG, Jeon TW, Oh MJ, Lee GH, Jeon JH. Effect of the ethanol extract of Lycium chinese on the oxgen free radical and alcohol metabolizing enzyme activities in rats. J Korean Soc Food Sci Nurt. 2000 ; 29(2) : 268-73.
19 Yang YK, Wang L, Kwon O. Protective effects of Acanthopanax koreanum Kakai extract against carbon tetrachloride-induced liver injury in Sprague- Dawley rats. J Nutr Health. 2014 ; 47(2) : 106-12. https://dx.doi.org/10.6116/kjh.2016.31.6.37.   DOI
20 Lee JY, Seo BI, Roh SS. Improving effect of Artemisiae Capillaris Herba extract in reflux esophagitis rats. Kor. J. Herbol. 2016 ; 31(6) : 37-44. https://dx.doi.org/10.6116/kjh.2016.31.6.37.   DOI
21 Zimmerman T. Franke H, Dargel R. Studies on lipid and lipoprotein metabolism in rat liver cirrhosis induced by different regiments of thioacetamide administration. Exp Pathol. 1986 ; 30(2) : 109-17.   DOI
22 Porter WR, Gudzinowicz MJ, Neal RA. Thioacetamide-induced hepatic necrosis. II. Pharmacokinetics of thioacetamide and thioacetamide-s-oxide in the rat. J Pharmacol Exp Ther. 1979 ; 208(3) : 386-91.
23 Wagner H. Antihepatotoxic flavonoids in plant flavonoids in biology and medicine: biochemical, pharmacological and structure-activity relations. Planta Med. 1992 ; 58(6) : 544-48.   DOI
24 Ramellini G, Meldoles J. Liver protection by silymarin: in vitro effect on dissociated rat hepatocytes. Arzneimittelforschung. 1976 ; 26(1) : 69-73.
25 Valenzuela A, Guerra R, Garrido A. Silybin dihemisuccinate protects rat erythrocytes against phenylhydrazine induced lipid peroxidation and hemolysis. Planta Med. 1987 ; 53(5) : 402-5.   DOI