• Title/Summary/Keyword: luxS

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A Bioassay for Chemicals Affecting Plant Pigment Biosynthesis: Greening Assay (식물색소 관여형 화합물의 생물검정법으로서 Greening Assay)

  • Kim, J.S.;Kim, T.J.;Hong, K.S.;Hwang, I.T.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.214-220
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    • 1990
  • To establish a greening assay for screening, and physiological and biochemical studies of the compounds affecting biosynthesis of plant pigments, were conducted on environmental factors, and on ways of incubation and illumination which affect plant greening. Greening was good when both cucumber and barley were grown for 5 to 6 days at $25^{\circ}C$ in darkness, when adaxial sides of cucumber cotyledons were contacted with the solution, and when barley leaf fragments were taken 0.5 to 2.0cm from the leaf tip. Potassium phosphate buffer(pH 6.0) at 10mM was most desirable for plant greening. The speed of greening during illumination was increased as the temperature increased from $15^{\circ}C$ to $35^{\circ}C$. The responses were sensitive more in cucumber than in barley, and in chlorophyll biosynthesis than in carotenoid biosythesis. The content of chlorophyll was greatest at the light intensity of 5000 and 1000 lux for cucumber and barley, respectively, but the biosynthesis of carotenoids were greatest at the light intensity higher than for chlorophyll. In use of solvents for dissolving chemicals, acetone, ethyl alcohol and DMSO at 10, 0.1 and 2.5% or less, respectively, did not affect the biosynthesis of plant pigments. $pI_{50}$ values were calculated for chemicals affecting pigment biosynthesis.

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Effects of Daylength Extension by Red Light in Strawberry Cultivation (적색광에 의한 딸기재배의 일장연장 효과)

  • Hong, Seung-Chang;Kim, Min-Kyeong;Kim, Myung-Hyun;Choe, Soon-Kun;Eo, Jin-Woo;Jung, Goo-Bok;So, Kyu-Ho
    • Korean Journal of Environmental Agriculture
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    • v.33 no.4
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    • pp.358-363
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    • 2014
  • BACKGROUND: Many strawberry growers are utilizing daylength extension by using incandescent bulb or fluorescent lamp to break dormancy of strawberry induced by low temperature and short day conditions. Conventional incandescent bulb and fluorescent lamp consume a lot of electricity and have short longevity. Red light known for most efficient wavelength for daylength extension light of short-day plant and long-day plant. This study was conducted to verify the effects of red light to enhance growth and to increase production of strawberry (Fragaria ${\times}$ ananassa Duch. cvs. "Seolhyang") METHODS AND RESULTS: Three red light (660nm) of 0.70, 0.87, and $1.05{\mu}mol/m^2/s$ (PAR) and conventional incandescent bulb of 40 Lux were treated respectively under the pot experiment. All treatment irradiated from 18:00 to 24:00 for 6 hours. Red light treatment tend to increase leaf stem number, flower stem number, weight of flower stem, crown weight, root weight, and leaf area of strawberry then incandescent bulb treatment. In field experiment, red light of $0.7{\mu}mol/m^2/s$ (PAR) and conventional incandescent bulb of 40 Lux were irradiated respectively. Field experiment showed that the leaf number, leaf weight, and crown weight of strawberry increased than those of incandescent bulb control with red LED of $0.7{\mu}mol/m^2/s$ (PAR). Red LED treatment increased the fruit number over 15g than incandescent bulb. Furthermore, red LED treatment decreased fruit number below 15g of strawberry than incandescent bulb treatment. Therefore, We believed that red LED treatment increased marketable fruit number by increment of weight of each fruit. Consequently, marketable fruit number, fruit weight, and fruit production of strawberry were increased than those of incandescent bulb by 5 %, 2.9 %, and 8.5 % respectively, but not showed significantly differences. CONCLUSION: These results presumably due to directly enhanced photosynthesis of strawberry leaves and activated action of Pfr phytochrome form by red light. In conclusion, red LED of 660nm could be used for daylength extension light source to enhance production of strawberry.

Illumination Simulation of the Daylight using AGI S/W Program (AGI 프로그램을 활용한 자연광 조도시뮬레이션)

  • Lee, Boong-Joo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.58-62
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    • 2017
  • In this study, the design conditions for the Korean-style glass greenhouse structure has been reduced to achieve the most efficient use of natural light. The AGI program was simulated for the optimal conditions of daylight in a glass greenhouse. From the results of daylight simulation, the axis position of the glass greenhouse's roof was not an important factor in the daylight effects regarding illumination and uniformity. In summer, there were long periods of daylight and high illumination levels. The illumination value of daylighting increased with increasing glass transparency value, and the illumination value was greatest at 14:00 hours. At this time, the rate of light variation according to the glass transparency was 89 [lux/%]. In addition, the optimal design conditions for the glass greenhouse were established, which were a $30[^{\circ}]$ or $150[^{\circ}]$ installation angle and higher transmittance of glass.

Composition of Fatty Acid and the Effect of Environmental Factors on the Population Growth of Scrippsiella trochoidea a Dinoflagellate Responsible for a Red Tide (적조와편모조 Scrippsiella trochoidea 군증식에 미치는 환경요인과 지방산 조성)

  • LIM Wol-Ae;KIM Hak-Gyoon;LEE Won-Jae;LEE Sam-Seuk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.2
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    • pp.198-203
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    • 1993
  • The cyst of Scrippsiella trochoidea from the surface mud in Masan Bay was germinated in the incubator for the culture. This species was one of dinoflagellates responsible for the early spring bloom in the southern coastal water of Korea. The culture experiments were carried out under the various gradients of environmental factors to know their effects on the population growth of this dinoflagellate. With respect to the effects of environmental factors on the growth, it was proved that the maximum cell growth was occurred at 4,000 lux of light intensity, salinity $30\%0$ and temperature $20^{\circ}C$. When 0.25ml/ml of the filtrates of Skeletonema costatum culture medium and the supernatants of soil extracts were added to growth medium as organic growth stimulants, both materials enhanced the population growth. In the fatty acid composition of S. trochoidea, $C_{16:0}$ was the major component, and $C_{18:0},\;C_{18:1},\;C_{22:0}\;and\;C_{22:1}$ were a minor components.

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Influence of the material for preformed moulds on the polymerization temperature of resin materials for temporary FPDs

  • Pott, Philipp-Cornelius;Schmitz-Watjen, Hans;Stiesch, Meike;Eisenburger, Michael
    • The Journal of Advanced Prosthodontics
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    • v.9 no.4
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    • pp.294-301
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    • 2017
  • PURPOSE. Temperature increase of $5.5^{\circ}C$ can cause damage or necrosis of the pulp. Increasing temperature can be caused not only by mechanical factors, e.g. grinding, but also by exothermic polymerization reactions of resin materials. The aim of this study was to evaluate influences of the form material on the intrapulpal temperature during the polymerization of different self-curing resin materials for temporary restorations. MATERIALS AND METHODS. 30 provisonal bridges were made of 5 resin materials: Prevision Temp (Pre), Protemp 4 (Pro), Luxatemp Star (Lux), Structure 3 (Str) and an experimental material (Exp). Moulds made of alginate (A) and of silicone (S) and vacuum formed moulds (V) were used to build 10 bridges each on a special experimental setup. The intrapulpal temperatures of three abutment teeth (a canine, a premolar, and a molar,) were measured during the polymerization every second under isothermal conditions. Comparisons of the maximum temperature ($T_{Max}$) and the time until the maximum temperature ($t_{TMax}$) were performed using ANOVA and Tukey Test. RESULTS. Using alginate as the mould material resulted in a cooling effect for every resin material. Using the vacuum formed mould, $T_{Max}$ increased significantly compared to alginate (P<.001) and silicone (P<.001). In groups Lux, Pro, and Pre, $t_{TMax}$ increased when the vacuum formed moulds were used. In groups Exp and Str, there was no influence of the mould material on $t_{TMax}$. CONCLUSION. All of the mould materials are suitable for clinical use if the intraoral application time does not exceed the manufacturer's instructions for the resin materials.

Solid Phase Synthesis of N-(3-hydroxysulfonyl)-L-homoserine Lactone Derivatives and their Inhibitory Effects on Quorum Sensing Regulation in Vibrio harveyi (고체상 합성법에 의해 합성된 N-(3-hydroxysulfonyl)-L-homoserine Lactone 유사체들의 Vibrio harveyi 쿼럼 센싱에 대한 저해 효과)

  • Kim, Cheol-Jin;Park, Hyung-Yeon;Kim, Jae-Eun;Park, Hee-Jin;Lee, Bon-Su;Choi, Yu-Sang;Lee, Joon-Hee;Yoon, Je-Yong
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.248-257
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    • 2009
  • The inhibitors against Vibrio harveyi quorum sensing (QS) signaling were developed by modifying the molecular structure of the major signal, N-3-hydroxybutanoyl-L-homoserine lactone (3-OH-$C_4$-HSL). A series of structural derivatives, N-(3-hydroxysulfonyl)-L-homoserine lactones (HSHLs) were synthesized by the solid-phase organic synthesis method. The in vivo QS inhibition by these compounds was measured by a bioassay system using the V. harveyi bioluminescence, and all showed significant inhibitory effects. To analyze the interaction between these compounds and LuxN, a 3-OH-$C_4$-HSL receptor protein of V. harveyi, we tentatively determined the putative signal binding domain of LuxN based on the sequence homology with other acyl-HSL binding proteins, and predicted the partial 3-D structure of the putative signal binding domain of LuxN by using ORCHESTRA program, and further estimated the binding poses and energies (docking scores) of 3-OH-$C_4$-HSL and HSHLs within the domain. In comparison of the result from this modeling study with that of in vivo bioassay, we suggest that the in silica interpretation of the interaction between ligands and their receptor proteins can be a valuable way to develop better competitive inhibitors, especially in the case that the structural information of the protein is limited.

A Study on the Variation of Physiology Signals based on EEG with Humidity (습도 변화에 따른 뇌파 기반 생체신호 변화에 관한 연구)

  • Kim, Myung-Ho;Kim, Jung-Min
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.62 no.1
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    • pp.50-55
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    • 2013
  • Subjects with 0.7[clo]'s amount of clothing were estimated on their thermal comfort, concentrativeness, heart rate variability, stress and fatigue degree when given variation in relative humidity to 30, 40, 50, 60, 70, and 80[RH%], in an environmental test room of temperature 25[$^{\circ}C$], illumination 1000[lux] and air velocity 0.02[m/sec], by using EEG, learning ability and HRV. At the result, it was at 50~60[RH%] of relative humidity that subject's thermal comfort and concentrativeness were at the highest while stress were at the lowest, and it was at 60[RH%] of relative humidity that heart rate variability was most stabilized. It was found that when temperature and humidity of the environmental test room are at 25[$^{\circ}C$] and 50~60[RH%], subject's productivity and psychological state are least affected.

Latent Charge Erasing Technique for a-Se Digital X-ray Detector (비정질 셀레늄 디지털 X선 검출기에 대한 잔류 전하 제거 기술)

  • Kang, S.S.;Choi, J.Y.;Park, J.K.;Cho, J.W.;Moon, C.W.;Choi, H.K.;Nam, S.H.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11b
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    • pp.505-508
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    • 2001
  • Currently there is much interested in removing latent charge that is caused to latent image effect and blurring of obtained image as well as reduction of x-ray conversion efficiency in digital radiography system. To remove latent charge a-Se film is irradiated by light with 3500 lux using halogen lamp and optical fiber. We measured dark current and photosensitivity to analyze removing effect of latent charge, then compared with and without light erasing method. The reduction of measured signal due to latent charge effect was 32.5 %, and the removal effect of latent charge by using light erase method was its 95.5 %.

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Measurement of Optical Purity for Commercially Avialable Naproxen Sold in 2013 (2013년 시판된 나프록센의 광학순도 측정)

  • Seo, Hae Chan;Song, Jung Suk;Ryoo, Sang Hyun;Lee, Sang Heon;Ryoo, Dong Hyun;Yu, Jeong Jae;Ryoo, Jae Jeong
    • Journal of the Korean Chemical Society
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    • v.58 no.2
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    • pp.179-185
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    • 2014
  • Commercial (S)-naproxen was racemized under strong basic condition. After checking the peak position of (R)- and (S)-naproxen by analysis the recemized naproxen, optical purity of 19 commercialized naproxens sold in 2013 in Korea were examined by chiral HPLC. The Chiralcel OD-H column, ChiralHyun-LE(S)-1 column and LUX-Cellulose-1 column were used as chiral stationary phases and the mixed eluent of hexane:isopropanol:acetic acid as 100:1:0.1 was used as a mobile phase with a flow rate of 1.0 mL/min. Each data was obtained from an average value of at least three different experiments for each sample and the relative standard deviation of them appeared very small. The average optical purity values obtained from three different chiral columns were very similar and the total average optical purity value (99.32%) of nineteen commercialized naproxens used in this study were larger than those of three years ago (98.17%).

A Study on the Improvement of Aquaculture Security System to Insure the Lawful Evidence of Theft (도적행위의 법적증거확보를 위한 양식장 보안 시스템 개선에 관한 연구)

  • Yim, Jeong-Bin;Nam, Taek-Keun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.13 no.4
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    • pp.55-63
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    • 2007
  • The Group Digital Surveillance System for Fishery Safety and Security (GDSS-F2S) is to provide the target tracking information and the target identification information in order to secure an huge aquaculture farm-field from a thief. The two information, however, is not enough to indict the thief due to the lack of lawful evidences for the crime actions. To overcome this problem, we consider the target image information as one of solutions after discussion with the effective countermeasure tools for the crime actions with scenario-based analysis according to the geological feature of aquaculture farm-field. To capture the real-time image for the trespassing targets in the aquaculture farm-field area, we developed the image capture system which is consists of ultra sensitive CCD(Charge-Coupled Device) camera with 0.0001 Lux and supplementary devices. As results from the field tests for GDSS-F2S with image capture system, the high definite images of the vehicle number plate and shape, person's actions and features are obtainable not only day time but also very dark night without moon light. Thus it is cleary known that the improved GDSS-F2S with image capture system can provide much enough lawful evidences for the crime actions of targets.

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