• Title/Summary/Keyword: luxS

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Illuminance Effects Affecting to Cognitive Ability of the Elderly (고령자의 인지력에 미치는 조도의 영향)

  • Kim, Myung-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.3
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    • pp.507-512
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    • 2019
  • To study how illuminance affects cognitive ability of the elderly, the elderly's EEG, concentration, HRV and vibra image were measured in a test room with temperature $25[^{\circ}C]$, relative humidity 50[RH%] and air flow speed 0.02[m/sec] by varying illuminance to 100[lux], 300[lux], 600[lux], 1000[lux] and 1500[lux]. Ten active elderly males were selected as subjects. Experiment condition was fixed as 1met of activity amount where the subject is seated and relaxed with cloth amount of 0.7clo. As a result, 1000[lux] was found out to be the most pleasant illuminance for the elderly, because $M{\beta}$ increased by 66.35%, and $S{\alpha}$ increased by 31.57% when the elderly was under 1000[lux] of illuminance. Also, concentration under 1000[lux] increased by 8.83% compared to 100[lux], and the pattern of concentration maintained uniformly. SDNN increased by 74.94% under 1000[lux] compared to 100[lux]. Nervousness decreased by 97.23% under 1000[lux] compared to 100[lux]. Moreover, HRT notably increased and aggression remarkably decreased under illuminance of 1000[lux]. Thus, based on the fact that comfort, concentration and heart stability of the elderly reach the highest under 1000[lux], it is determined that the illuminance has to be considered foremost in designing the elderly's welfare facilities to raise their safety and level of independence.

Expression of the Genes Involved in the Synthesis of Riboflavin from Photobacterium species of Bioluminescent Marine Bacteria (해양 발광 박테리아 Photobacterium Species의 Riboflavin 생합성에 관여하는 유전자들의 발현)

  • 이찬용
    • Korean Journal of Microbiology
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    • v.36 no.1
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    • pp.1-7
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    • 2000
  • The genes involved in riboflavin synthesis (ribI, II, III, and IV) were found immediately downstream of luxG in the lux operon from Photobacterium species. The single stranded DNA containing the intergenic region of lux genes and rib genes from Photobacterium phosphoreum was fully protected by P. phosphoreum mRNA from the S1 nuclease mapping assay suggesting that a transcriptional terminator was not present in the region. In addition, the levels of riboflavin synthase activity in P. phosphoreum was increased during the development of bacterial bioluminescence in the same fashion as the luciferase and fatty acid reductase activities. Insertion of the Photobacterium leiognathi DNA extending from luxB to ribII, between a strong lux promoter and a reporter gene (chloramphenicol acetyltransferase, CAT) and transferred by conjugation into P. leiognathi, did not affect expression of reporter gene. Moreover the CAT gene was not expressed in an analogous construct missing the lux promoter indicating that a promoter was not present in this region. Based on the data here, it can be concluded that the lux genes and rib genes in Photobacterium species are under common regulation.

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The Study on Envronmental Sanitation for Night High School. -Illuminate- (야간 고등학교의 환경위생학적 조사 -조명을 중심으로-)

  • 김난천;오석흔
    • Journal of Environmental Health Sciences
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    • v.5 no.1
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    • pp.40-45
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    • 1978
  • We have selected 36 schools of a total of night high schools for boys and girls in seoul and measured intensity of illumination of the classroom, The corridors and the stairs that students study and live, with priority given to an illumination, a primary factor of environmental sanitation of school following is the result. 1. The maximum average intensity of illumination of the classroom is 93.2 Lux, and the minimum average intensity of illumination 39.5 Lux. Mean$\pm$S, is 59.03$\pm$22.8 Lux 2. The maximum average intensity of illumination of the corridor is 39.2 Lux, and the minimum average intensity of illumination 11.1 Lux. 3. The maximum average intensity of illumination of the stair is 11.58 Lux, and the minimum average intensity, of illumination 4.92 Lux, mean$\pm$S.D is 7.88$\pm$10.0Lux. 4. Schools with tile illumination facilities more than 50 Lux are 63.8% and less than 50 Lux are 36.2%. 5. Schools with 9-11 facilities of a source of light per classroom by a fluorescent lamp are the most as 30.50%. 6. As for the corridor, schools with the illumination equipment less than 10 Lux are 27.8%, are more than 10 Lux 72.2% 7. As for the stairs schools with the illumination equipment less than 10 Lux are 77.8%, and more than 10 Lux 10 Lux 22.2%.

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Expression and DNA Sequence of the Gene Coding for the lux-specific Fatty Acyl-CoA Reductase from photobacterium phosphoreum

  • Lee, Chan-Yong;Edward A. Meighen
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.80-87
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    • 2000
  • The nucleotide sequence of the luxC gene coding for lux-specific fatty acyl-CoA reductase and the upstream DNA (325bp)of the structural gene from bioluminescent bacterium, Photobacterium phosphoreum, has been deternubed. An open reading frame extending for more than 20 codons in 325 bp DNA upstream of luxC was not present in both directions. The lux gene can be translated into a polypeptide of 54 kDa and the amino acid sequences of lux specific reductases of P. phosphoreum shares 80, 65, 58, and 62% identity with those of the Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, and Xehnorhabdus luminescenens reductases, respectively. Analyses of codon usage, showing that a high frequency (2.3%) of the isoleucine codon, AUA, in the luxC gene compared to that found in Escherichia coli genes (0.2%) and its absence in the luxA and B genes, suggested that the AUA codon may play a modulator role in the expression of lux gene in E. coli. The structural genes (luxC, D, A, B, E) of the P. phosphoreum coding for luciferase (${\alpha}$,${\beta}$) and fatty acid reductase (r, s, t) polypeptides can be expressed exclusively in E. coli under the T7 phage RNA polymerase/promoter system and identificationof the [35S]methionine labelled polypeptide products. The degree of expression of lux genes in analyses of codon usage. High expression of the luxC gene could only be accomplished in a mutant E. coli 43R. Even in crude extracts, the acylated acyl-CoA reductase intermediate as well as acyl-CoA reductrase activities could be readily detected.

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Effect of Scrapping Aerial Mycelia and Light on the Production of Macroconidia and Chlamydospores of Cylindrocarpon destructans Causing Root Rot of Panax ginseng (기중균사 제거와 광처리가 인삼 뿌리썩음병균 Cylindrocarpon destructans의 대형분생포자 및 후막포자 생성에 미치는 영향)

  • Cho Dae-Hui;Yu Yun-Hyun;Ohh Seung-Hwan
    • Journal of Ginseng Research
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    • v.23 no.3 s.55
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    • pp.123-129
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    • 1999
  • Under the light condition of 25,000 Lux (12 hrs dark and light cycle) with scrapping treatment of aerial mycelia of Cylindrocarpon destructans on potato dextrose agar (PDA), V-8 juice agar, and ginseng extract agar, production of the macroconidia was increased to $3.7\~8.1$ fold over them produced in the dark. They were also produced $7.7\~18.0$ times more in the liquid cultures under the light condition than under the dark as well. PDA and V-8 juice agar among the tested were the best for the macroconidium production. On PDA, 1,585 $macroconidia/mm^2$ were produced under the light of 25,000 Lux with scrapping treatment of aerial mycelia of C. destructans, which is 3.2 and 1.4 times more than those produced under 3,000 and 10,000 Lux, respectively. Meanwhile, $20\~99$ macroconidia/$mm^2$ were produced by the non-scrapping under the light condition between 3,000 Lux and 25,000 Lux. The macroconidia were, however, lysed at $6\~7$ days after being incubated under the above range of the light. They were consisted of $1\~3$ cells in a macroconidium while $69.4\~100\%$ of them were the two-celled and the number did not seem to be affected by either the scrapping or the light. Production of chlamydospore converted from mycelia of C. destructans seemed to be promoted by the light and the scrapping as well. The 1,285 chlamydospres/$mm^2$ were produced with the light (25,000 Lux), which is 2.8 and 1.2 times more than those with 3,000 and 10,000 Lux, respectively. Scrapping the aerial mycelia of the cultures increased the chlamydospore formation to 1.9, 2.5 and 1.4 times more than the non-scrapping under the light intensity of 3,000 Lux, 10,000 Lux, and 25,000 Lux, respectively. On PDA, 1 to 8 chlamydospore(s) per catena were formed by all treatments tested and $34.2\~58.9\%$ of them was a single chlamydospore, However, the numbers was affected by neither the light ($3,000\~25,000$ Lux) nor the scrapping the aerial mycelia.

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luxS and smcR Quorum-Sensing System of Vibrio vulnificus as an Important Factor for In Vivo Survival

  • SHIN NA-RI;BAEK CHANG-HO;LEE DEOG-YONG;CHO YOUNG-WOOK;PARK DAE-KYUN;LEE KO-EUN;KIM KUN-SOO;YOO HAN-SANG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.6
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    • pp.1197-1206
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    • 2005
  • Vibrio vulnificus is an opportunistic pathogen that causes a septicemia and expresses numerous virulence factors, in which luxS and smcR are genes encoding for components responsible for quorum-sensing regulation. In the present study, null mutants were constructed with lesions in each or both of these two genes from the V. vulnificus Vv$\Delta$Z strain, which is a lacZ$^{-}$ and chloramphenicol/streptomycin-resistant derivative of the wild-type ATCC29307 strain, and their phenotypes related to virulence were compared with those of the parental cells. $LD_{50}$ and histopathological findings of luxS-, smcR-, or luxS- smcR- deficient mutant were not different from those of the parent strain, a lacZ-deficient streptomycin-resistant strain in mice. However, time of death in mice was delayed, and numbers of bacteria survived in bloodstream after intraperitoneal injection in mice were decreased by mutation, especially luxS and smcR double mutant (VvSR$\Delta$ZSR). These phenomena were supported by increased serum sensitivity and delayed bacterial proliferation in both murine blood and iron-restricted medium. These results suggest that the luxS and luxR homologous genes in V. vulnificus could playa role in bacterial survival in host by enhancing proliferation and adjusting to changed environment.

Intraspecific Variation in the Light Intensity Niche Component of the Diatom Skeletonema Costatum from Korean Coastal Waters (한국연안 산 규조 Skeletonema Costatum 의 조도에 대한 생태적지위 성분의 종내 변이)

  • YIH, WONHO;SHIM, JAE HYUNG
    • 한국해양학회지
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    • v.30 no.5
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    • pp.436-441
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    • 1995
  • Final biomass yields (peak optical density) and growth rates (divisions/day) of seven clones of Skeletonema costatum from Korean coastal waters were measured to understand their intraspecific variations in the light intensity niche component under 25$^{\circ}C$ condition. Daily growth rates of 6 out of 7 S. costatum clones were maximum at 6000 lux while that of YS4, a neritic clone, was maximum at 9000 lux. The final biomass yields of 4 out of the 7 s. costatum clones were maximum at the lowest light intensity of 2000 lux. Minimum final biomass yields were found at 9000 lux in all the S. costatum clones other than an estuarine clone, HDC9. The intraspecific variations of the mean growth rate and mean final biomass yield under each of the three different light intensities in terms of the coefficient of variation were not greater than 10% in any of the 7 S. costatum clones.

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Photosynthetic membranes of Rhodocyclus gelatinosus and Rhodocydlus tenuis (Rhodocyclus gelatinosus와 Rhodocyclus tenuis의 광합성막에 관하여)

  • 이현순
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.144-147
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    • 1987
  • Intracytoplasmic photosynthetic membranes of Rhodocyclus gelatinosus and Rhodocyclus tenuis are known to be of weakly developed type of in the Rhodospirillaceae. We compared the membrane invagination of Rhodocyclus gelatinosus KS-117 (isolated in lur laboratory) with that of Rhodocyclus tenuid (ATCC 25093) after culturing under several different light intensities. We observed the significant membrane invagination in Rhodocyclus gelatinosus, in particular under 3,000 Lux, while none was observed in Rhodocyclus tenuis.

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Measurement of Iron-dependence of pupA Promoter Activity by a pup-lux Bioreporter

  • Khang, Yong-Ho;Yang, Zamin-K.;Burlage, Robert-S.
    • Journal of Microbiology and Biotechnology
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    • v.7 no.5
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    • pp.352-355
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    • 1997
  • The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-dependence of pupA promoter activity. Factors affecting bioluminescence produced by the pup-lux bioreporter were found to be cell activity, iron-chelator concentrations, Fe(III) concentrations, and nutrient components. Light production rates of the pup-lux bioreporter were inversely dependent upon iron molecules when $FeCl_3$ concentrations were between $10^{-2}$ and 1 ${\mu}M$ in nutrient-poor minimal media, and between 0.1 and 10 mM in nutrient-rich complex media.

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Intraspecific Variation in the Temperature Niche Component of the Diatom Skeletonema costatum from Korean Coastal Waters

  • YIH Wonho;SHIM Jae Hyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.6
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    • pp.805-811
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    • 1995
  • Final biomass yields (peak optical density) and growth rates (divisions/day) of seven clones of Skeletonema costatum from Korean coastal waters were measured to understand their intraspecific variations in the light intensity niche component under $25^{\circ}C$ condition. Daily growth rates of 6 of 7 S. costatum, clones were maximum at 6000 lux while that of YS4, a neritic clone, was maximum at 9000 lux. The final biomass yields of 4 of the 7 S. costatum clones were maximum at the lowest light intensity of 2000 lux. Minimum final biomass yields were found at 9000 lux in all the S. costatum clones other than an estuarine clone, HDC9. The intraspecific variations of the mean growth rate and mean final biomass yield under each of the three different light intensity in terms of the coefficient of variation were not greater than 10% in any of the 7 S. costatum clones.

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