Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Expression of the Genes Involved in the Synthesis of Riboflavin from Photobacterium species of Bioluminescent Marine Bacteria

해양 발광 박테리아 Photobacterium Species의 Riboflavin 생합성에 관여하는 유전자들의 발현

  • Published : 2000.03.01
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Abstract

The genes involved in riboflavin synthesis (ribI, II, III, and IV) were found immediately downstream of luxG in the lux operon from Photobacterium species. The single stranded DNA containing the intergenic region of lux genes and rib genes from Photobacterium phosphoreum was fully protected by P. phosphoreum mRNA from the S1 nuclease mapping assay suggesting that a transcriptional terminator was not present in the region. In addition, the levels of riboflavin synthase activity in P. phosphoreum was increased during the development of bacterial bioluminescence in the same fashion as the luciferase and fatty acid reductase activities. Insertion of the Photobacterium leiognathi DNA extending from luxB to ribII, between a strong lux promoter and a reporter gene (chloramphenicol acetyltransferase, CAT) and transferred by conjugation into P. leiognathi, did not affect expression of reporter gene. Moreover the CAT gene was not expressed in an analogous construct missing the lux promoter indicating that a promoter was not present in this region. Based on the data here, it can be concluded that the lux genes and rib genes in Photobacterium species are under common regulation.

발광 박테리아인 Photobacterium 종들의 lux 오페론 하부 영역에서 riboflavin 생합성에 관여하는 유전자들(ribⅠ,Ⅱ,Ⅲ,Ⅳ)이 발견되었다. Photobacterium phosphoreum의 lux 유전자와 rib 유전자를 포함하는 intergenic 영역의 단일사슬 DNA가 P. phosphoreum의 mRNA에 의하여 S1 nuclease digestion에서 손상받지 않았으며, ribⅠ에 의하여 암호화되는 P. phosphoreum의 riboflavin synthase의 활성도가 lux-specific한 효소들인 luciferase 혹은 fatty acid reductase 활성도와 같이 bioluminescence intensity의 발현과 함께 대수기 말기에서 증가하는 박테리아 발광반응의 특이한 조절 체계인 'autoinduction' 양상을 보였다. 또한 P. leiognathi의 luxB로부터 ribⅡ까지 포함하는 DNA를 강력한 lux 프로모터와 reporter(chloramphenicol acetyl transferase, CAT) 유전자 사이에 삽입하고 접합(conjugation)의 방법으로 P. leiognathi에 유전자 전이(gene transfer)시켜 CAT reporter 유전자의 발현을 P. leiognathi에서 조사한 바, 그 유전자의 발현 정도에 큰 차이가 없었을 뿐만 아니라 이 구조에서 lux 프로모터를 제거하게 되면 CAT reporter 유전자의 발현이 전혀 나타나지 않았다. 이들 실험 결과들은 lux 유전자와 rib 유전자의 intergenic영역에 lux 오페론의 전사 종결 구조(transcriptional terminator)가 존재하지 않으며 ribflavin 생합성 유전자들이 그들 고유의 프로모터에 의하여 전사되는 것이 아니라 lux 오페론의 프로모터에 의하여 발현됨을 나타내는 것으로, 이는 Photobacterium 종들에서 lux 유전자와 rib 유전자들은 공동의 발현 조절 체계를 갖는 것으로 요약된다.

Keywords

References

  1. FEBS Lett. v.302 The haemoglobin-like protein (HMP) of Escherischia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin $NADP^+$ reductases Andrew, S.C.;D.Shipley;J.N. Keen;J.B.C. Findlay;P.M. Harrison;J.R. Guest
  2. Proc. Natl. Acad. Sci. USA v.76 Replication of an origincontaining derivative of plasmid RK2 dipendent on a plasmid function provided in trans Figurski, D.H.;D.R. Hellinski
  3. Annu. Rev. Microbiol. v.50 Census and concensis in bacterial ecosystem: the luxR-luxI family of quprum sensing transcriptional regulators Fukua, C.;S.C. Winans;E.P. Greenberg
  4. The phamacological basis of therapeutics(8th ed.) Water Soluble Vitamin Gilman, A.G.;Gilman(ed.);Rall(ed.);Nies(ed.);Tayler(ed.)
  5. J. Opt. Soc. Am v.53 Total quantum flux of isotropic sources Hastings, J.W.;G. Weber
  6. Adv. Microbiol. Physiol v.26 Biochemistry and physiology of bioluminescent bacteria Hastings, J.W.;C.J. Potrikus;S.C. Gupta;M. Kurfurst;J.C. Makemson
  7. Bioluminescence in Action Herring, P.J.;Herring(ed.)
  8. J. Biolumin. Chemilumin v.1 Systematic distribution of bioluminescence in living organisms Herring, P.J.
  9. J. Bacteriol v.163 Diffusion of autoinducer is involved in regulation of the Vibrio fischeri luminscence system Kaplan, H.B.;E.P. Greenberg
  10. Eur. J. Biochem v.201 The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish Lee, C.Y.;R.B. Szittner;E.A. Meighen
  11. Biochem. Biophys. Res. Commun. v.186 The lux genes in Photobacterium leiognathi are closely linked with genes corresponding in sequence to riboflavin synthesis genes Lee, C.Y.;E.A. Meighen
  12. J. Bacteriol v.176 Riboflavin synthesis genes are linked with the lux operon of Photobacterium phosphoreum Lee, C. Y.;D. O'Kane;E.A. Meighen
  13. J. Biol. Chem. v.263 Cloning and expression of the Photobacterium phosphoreum luminescence system demonstrates a unique lux gene organization Mancini, J.A.;M. Boylan;R.R. Soly;A.F. Graham;E.A. Meighen
  14. Annu. Rev. Microbiol v.42 Enzymes and genes from the lux operons of bioluminescent bacteria Meighen, E.A.
  15. Microbiol Rev. v.55 Molecular biology of bacterial bioluminescence Meighen, E.A.
  16. J. Bacteriol v.172 Transcriptional regulation of the lux genes transferred into Vibrio harveyi. Miyamoto,C.;E. Meighen;A. Graham
  17. Proc. Natl. Acad. Sci. USA v.88 Borrowed proteins in bacterial bioluminescence O'Kane, D.J.;B. Woodward;J. Lee;D.C. Prasher
  18. J. Biol. Chem v.254 Coinduction of fatty acid reductase and luciferase during development of bacterial luminescence Riendeau, D.;E. Meighen
  19. Dictonary of microbiology and molecular biology(2nd ed.) Riboflavin Singleton, P.;P. Sainsbury
  20. J. Biol. Chem. v.265 Delineation of the transcriptional boundaries of the lux operon of Vibrio harveyi demonstrates the presence of two new lux genes Swartzman, E.;C. Miyamoto; A, Graham;E. Meighen
  21. Proc. Natl. Acad. Sci. USA v.82 A bacteriophage T7 RNA polymerase-promoter system for controlled exclusive expression of specific genes Tabor, S.;C.C. Richardson