• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.688-688
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• 2003

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.681-681
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• 2003

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.645-646
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• 2003

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.139-144
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• 2016

Synthetic oscillators are gene circuits in which the protein expression will change over time. The delay of transcription, translation, and protein folding is used to form this kind of behavior. Here, we tried to design a synthetic oscillator by a negative feedback combined with a positive feedback. With the mutant promoter P_LacC repressed by LacIq and P_Lux activated by AHL-bound LuxR, two gene circuits, Os-LAA and Os-ASV, were constructed and introduced into LacI-deleted E. coli DH5α cells. When glucose was used as the carbon source, a low level of fluorescence was detected in the culture, and the bacteria with Os-ASV showed no oscillation, whereas a small portion of those carrying Os-LAA demonstrated oscillation behavior with a period of about 68.3 ± 20 min. When glycerol was used as the carbon source, bacteria with Os-ASV demonstrated high fluorescence value and oscillation behavior with the period of about 121 ± 21 min.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.66-71
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• 2016

PikD is a widely known pathway-specific regulator for controlling pikromycin production in Streptomyces venezuelae ATCC 15439, which is a representative of the large ATP-binding regulator of the LuxR family (LAL) in Streptomyces sp. RapH and FkbN also belong to the LAL family of transcriptional regulators, which show greatest homology with the ATP-binding motif and helix-turn-helix DNA-binding motif of PikD. Overexpression of pikD and heterologous expression of rapH and fkbN led to enhanced production of pikromycin by approximately 1.8-, 1.6-, and 1.6-fold in S. venezuelae, respectively. Cross-complementation of rapH and fkbN in the pikD deletion mutant (ΔpikD) restored pikromycin and derived macrolactone production. Overall, these results show that heterologous expression of rapH and fkbN leads to the overproduction of pikromycin and its congeners from the pikromycin biosynthetic pathway in S. venezuelae, and they have the same functionality as the pathwayspecific transcriptional activator for the pikromycin biosynthetic pathway in the ΔpikD strain. These results also show extensive "cross-communication" between pathway-specific regulators of streptomycetes and suggest revision of the current paradigm for pathwayspecific versus global regulation of secondary metabolism in Streptomyces species.

• Journal of the Korean Solar Energy Society
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• v.37 no.1
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• pp.39-45
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• 2017

This paper presents the effect of solar collectors on the performance of solar tracking daylighting systems. A series of measurements were made for two different types of solar collectors mounted on double axis solar trackers: Parabolic dish concentrator and Fresnel Lens. Indoor light levels were measured at different locations of an office space (longitude: 126.33 E, latitude 33.45 N) as photo sensors were placed on a task plane 80 cm above the floor. To accurately monitor the applicability of the systems, measurements were performed under clear and overcast sky conditions with the roll-screen (on the south window) in the down position. Comparing the illuminance data, the system with Fresnel lens outperformed that of parabolic dish concentrator. On clear days, the former delivered the light levels of 400~600 lux on the task plane whereas the latter recorded 100~200 lux. Depending on the amount of cloud cover, on overcast days, illuminance readings fluctuated to some extent.

• Journal of environmental and Sanitary engineering
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• v.11 no.1
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• pp.83-91
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• 1996

It is not too much to say that the territorial inhabitants' concerns are wholly c concentrated on the environmental preservation-problem and development-problem in Korea given effect to the local self-government system. At a time like this I was studied the effect on nutrients removal through lighting period in aerobic high rate pond and we know that waste stabilization pond method is the most economical and energy saving wastewater treatment technology than others. At the results which was studied through operating the reactor-l artifically main-tained at a temperature, $25^{\circ}C$, a light intensity, 3000lux, and a lighting period, 24hrs and the reactor-2 artifically maintained at a tern야rature, $25^{\circ}C$ and a light intensity 3000lux, and a lighting period period, 12hrs, It has appeared for 24hrs.-lighting period -reactor-1 to be prior to the reactor-2. The attained results are that 1. reactor-1 is prior to reactor-2 on oxygen-generation 2. reactor-1 is prior to reactor-2 on algal production 3. COD removal efficiency, 90.76%, T-N removal efficiency, 80%, T-P removal e efficiency, 74.47 % in reactor-2, in reactor-1 COD removal efficiency, 94.85 %, T-N removal efficiency, 98.07%, T-P removal efficiency, 72.13% are, so the treatment efficiency of reactor-1 is more excellent than things of reactor-2 4. it appeared that the detention time is 8, 9days.

• Korean Journal of Microbiology
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• v.41 no.3
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• pp.236-238
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• 2005

The effect of light on production of cordycepin (3'-deoxyadenosine) was investigated using Cordyceps militaris. Among Cordyceps spp. tested, C. militaris KCTC 6064 was the best for cordycepin production, After 120-hr cultivation of C. militaris KCTC 6064 at $24^{\circ}C$ in YMG broth with light (1,000 lux), 51.6 mg/l of cordycepin was produced. In general, production of cordycepin with light illumination was better than that without light. Cordycepin production under light illumination was enhanced up 96-hr cultivation, whereas after 96-hr, cordycepin production was decreased.

• Korean Journal of Head & Neck Oncology
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• v.26 no.2
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• pp.187-192
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• 2010

Background and Objectives : LED(Light emitting diode) is recently introduced as a energy-saving light source in many area including agriculture and environment. In medical field it is known as bright and safe light source in surgical lighting including headlight. This study is aimed to test effectiveness and cost-saving of mountain-climbing headlight in comparison with xenon headlight. Materials and Methods : Internet market-available mountain-climbing headlight was compared with medical xenon headlight regarding heat generation after 30 minutes' usage, intensity of illumination and possible burn to the perioral skin. To get temperature data, 5 cases of tonsillectomy were done with the aid of LED headlight, while another 5 tonsillectomies were done using xenon headlight. Results : The temperatures of all light sources were below 45 degrees Celcius until finish of the surgery without burn or complications. No differences in operation time with both headlights. The maximal intensities of illumination were 24000 Lux for xenon, 20000 Lux for LED. Conclusion : Mountain-climbing headlight could be safe and helpful light source with low cost in simple oral surgery.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1125-1131
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• 2006

A kan'::luxCDABE fusion strain that was both highly bioluminescent and responsive to benzoic acid was constructed by transforming E. coli strain W3110 with the plasmid pUCDK, which was constructed by digesting and removing the 7-kb KpnI fragment from the promoterless luxCDABE plasmid pUCD615. Experiments using buffered media showed that this induction was dependent on the pH of the media, which influences the degree of benzoic acid protonation, and the expression levels seen are likely due to acidification of the cytoplasm by uncoupling of benzoic acid. Consequently, the sensitivity of this strain for benzoic acid was increased by nearly 20-fold when the pH was shifted from 8.0 to 6.5. Benzoic acid derivatives and several phenolics also resulted in significantly increased bioluminescent signals. Although these compounds are known to damage membranes and induce the heat-shock response within E. coli, bacterial strains harboring mutations in the fadR and rpoH genes, which are responsible for fatty acid biosynthesis during membrane stress and induction of the heat-shock response, respectively, showed that these mutations had no effect on the responses observed.