• Journal of Microbiology and Biotechnology
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• 제12권6호
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• pp.872-877
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• 2002

The hypolipidemic effect of the exe-biopolymer (EXBP) and endo-biopolymer (ENBP) produced from a submerged mycelial culture of Ganoderma lucidum was investigated in dietary-induced hyperlipidemic rats. Hypolipidemic effects were achieved in both the EXBP- and ENBP-treated groups, however, the former proved to be more potent than the latter. The administration of the EXBP (100 mg/kg body weight) substantially reduced the plasma total cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, phospholipid levels, and atherogenic index by 31.0, 39.0, 35.4, 28.1, and 53.5%, respectively, when compared to the control group. The EXBP also lowered the liver total cholesterol, triglyceride, and phospholipid levels by 22.4, 23.1, and 12.9%, respectively. Furthermore, the high-density lipoprotein (HDL) cholesterol and ratio of HDL cholesterol to total cholesterol were significantly increased by as much as 24.2% and 47.6%, respectively.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.115-119
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• 1994

White mutant of Ganoderma lucidum(G4142) induced the non-basediocarpous basidiospores(NBB) from the aerial mycelia on agar media by the light illumination. Light was found to be necessary for NBB formation, but it also inhibited the growth of mycelium. The best sporulation was obtained at the periodic exposure of 16 hour light and 8 hour dark. Blue and yellow light were the most effective on sporulation, however, near UV and red light did not induce any spores. Effective light intensity for NBB bearing was about 1,000 lux as white light. Even after 16 days of culture, this strain did not form the pinhead nor chlamydospore. Optimum temperature for the mycelial growth and NBB formation were 30$\circ $C. Ganoderma lucidum G4142 exhibited the formation of stroma after five days of incubation at 30$\circ $C.

• 한국환경과학회지
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• 제16권1호
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• pp.21-26
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• 2007

The concentrations of fourteen mineral and trace elements (Al, Ca, Fe, K, Mg, Se, Ba, Co, Cu, V, Pb, Hg, Cd and As) were determined in Ganoderma Lucidum and their infusions consumed for medical purposes collected from Vietnam, China and some places in Korea. Concentrated acid digestion procedure was applied under optimized conditions for dissolution of these medicinal fungi. Element concentrations in these fungi and their in-fusions were then determined by ICP-AES. The mineral and trace element content of these samples and their in-fusions showed a wide variability However, distribution of some elements in the infusions is not high.

• 한국버섯학회지
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• 제2권2호
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• pp.45-48
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• 2004

The effect of $CO_2$ concentration (500, 3,000, $6,000{\mu}{\ell}/{\ell}$) on the mycelial growth and fruit body primordium formation of Ganoderma lucidum on nutrient agar medium was examined. Optimum $CO_2$ concentration for vegetative growth was above $3,000{\mu}{\ell}/{\ell}$. Fruit body initiation was accelerated at higher than $3,000{\mu}{\ell}/{\ell}$ $CO_2$ exposure but the maximum number and size of primordia, and primordium color were not influenced by $CO_2$ concentrations. Whereas each atypical fruiting structure forming stock culture showed different fruiting time under each concentration of $CO_2$ exposure.

• 한국가정과학회지
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• 제7권3호
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• pp.49-58
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• 2004

The purpose of the study was to verify if or not as funtional food by estimating antitumor activity and antimicrobial activity of Coriolus versicolor(Fr.)Quel and Ganoderma Lucidum (Fr.)Karst. 1. The contents of moisture. crude fiber and crude protein of GL were higher $(18.28\%,\;10.3\%,\;78.4\%)$ than that of CV, but the content of crude lipid GL was higher than that of CV. 2. Inhibitory effects of on the growth of AGS human gastric adenocarcinoma cells and HT-29 human colon adenocarcinoma cell were increased by increasing of concentration in added methanol extracts of CV and GL. 3. Antimicrobial activity on Escherichia col1; Staphylococcus aureus, Salmonella typhimurium existed methanol extracts of CV. Therefore. it is suggested that CV is worth as functional food.

• 한국응용과학기술학회지
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• 제7권1호
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• pp.81-85
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• 1990

Saccharomyces Cerevisiae was cultured in the malt wort added 0%, 0.001%, 0.01%, 0.1% Chloroform - Methanol and Hexane extract of Ganoderma Lucidum. The more the quantity of Ganoderma Lucidum extract were added the more total alcohol quantity were produced during the culture. Especially higher alcohol contents were more increased about 2.2 times 0.1% added group than on control group 120 hr cultured. Ethanol product was more increased on Hexane extract added group than on Chloroform - Methanol extract added group.

• Natural Product Sciences
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• 제8권2호
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• pp.52-54
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• 2002

The cultured mycelia of fungus Ganoderma lucidum were investigated for the inhibitory effect on the growth of s.c. transplanted Lewis lung carcinoma (LLC) in BDF-1 mice by intraperitoneal (i.p.) administration. The cultured mycelia showed antitumor activity with T/C values of 89.6 and 50.3 % at doses of 100 and 500 mg/kg, respectively, compared to adriamycin, which was used a positive control, with T/C value of 54.6 % at 2 mg/kg.

• 미생물학회지
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• 제40권3호
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• pp.226-229
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• 2004

염색질을 구성하는 histone 단백질 lysine 잔기에 histone acetylase에 의하여 결합된 acetyl기를 제거하는 histone deacetylase (HDAC)는 진핵세포 생물의 염색질 안정 파 및 유전자 발현에 매우 큰 영향을 미친다. 국내에서 분리된 영지의 HDAC 유전자를 클로닝 하고자 cDNA 및 genomic DNA를 대상으로 PCR을 수행한 결과 470bp의 cDNA유전자와, 585 bp, 589 bp 및 630 bp길이의 genomic DNA유전자 조각을 클로닝 하였다. 이들의 염기서열을 근거로 아미노산 서열을 다른 균류의 HDAC와 비교한 결과 59-72％의 상동성을 보였다.

• Archives of Pharmacal Research
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• 제22권5호
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• pp.515-519
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• 1999

The biologically active peptidoglycan was purified form the alkali fraction of the fruiting bodies of Ganoderma lucidum and the composition of the peptidoglycan was investigated by conventional analyses. The alkali-extracted peptidoglycan showed differences in chemical compositions from the water-extracted. The alkali-extracted peptidoglycan contained 6.9% protein and 75.9% carbohydrates composed mainly of $\beta$-glucose, mannose, and $\alpha$-glucose. The molecular weight range of the peptidoglycan was determined as 2,000 kDa-17 kDa. The peptidoglycan is considered to be a hybrid molecule of polysaccaride chains covalently bound as a side chain to the polypeptide core.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.438-442
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• 1994

Antimicrobial activity of GL (the aqueous extract from the carpophores of Ganoderma lucidum ($F_RK_{KARST}$) was tested in vitro aginst Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration (MIC). Among fifteeen species of bacteria tested, the natimicrobial activity of GL was of antimicrobial combinations of GL with four kinds of antibiotics (ampicilin, cefazolin, oxytet-racycline and chloramphenicol), the fractional inhibitory concentraction index (FICI) was determined by checkerboard assy for each stain. The antimicrobial combinations of GL with four antibiotics resulted in additive effect in most instances, synergism in two instances, and antagonism in two instances. Synergism was obversed when GL was combined with cefazolin against Bacillus subtilis and Klebsiella oxytoca.