• Journal of Applied Biological Chemistry
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• v.48 no.2
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• pp.84-88
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• 2005

To develop functional food and anti-obesity drug through inhibition of dietary lipid absorption, inhibitory effects of herb extracts on pancreatic lipase were investigated. Due to high yield and simplicity of isolation, lipase inhibitor (ELI) was isolated from ethyl acetate extract of Eugenia aromaticum, which showed highest inhibitory activity, and characterized for development of novel functional material. Stability of ELI at high temperature and low pH was investigated. Results showed ELI is relatively stable under thermal and acidic conditions, reversible, and noncompetitive inhibitor of pancreatic lipase.

• Plant Disease and Agriculture
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• v.1 no.2
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• pp.18-21
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• 1995

Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.279-282
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• 2011

This study was carried out to development the environment-friendly control method of Myzus persicae using Beauveria bassiana and Environment-friendly agricultural materials (EFAM). Germination rate the dried conidia of B. bassiana was 90 percent at $24{\sim}27^{\circ}C$ temperature condition. Mortality of M. persicae was 56.7% at $1{\times}10^7$ cfu/ml conidia concentration. Four EFAM such as Pachungtan, Wangjungwang, Newbiodakill, Muginchon were low toxicity to B. bassiana growth. Among them, Muginchon (derris extract) and Wangjungwang (neem extract) could have synergistic effect to aphid mortality when mixing treat with B. bassiana, which protection value was 99% in 3 days after treat.

• Journal of Food Hygiene and Safety
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• v.13 no.1
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• pp.6-13
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• 1998

This study was carried out to investigate the antioxidative activity on oxidaton of human low density lipoprotein from marine microorganisms. Bacillus sp. RH-5 producing antioxidative activity have been isolated and identified from coast sea in Pusan. Bacillus sp. RH-5 produced at highest level of antioxidative activity in the medium of 1.0% glucose, 0.25% polypepton, 0.25% yeast extract, 0.01% $FeSO_4{\cdot}7H_2O$ and 50% sea water. The optimal medium pH, cultural temperature and shaking time for the highest production as the antioxidant were 7.0, $30^{\circ}C$ and 48 hr, respectively. The culture broth inhibited the copper catalyzed oxidation of human low density lipoprotein (LDL) at the concentration of 500 and $1,000\;\mu\textrm{g}/ml$ ethylacetate extracts in the presence of $5\;\mu\textrm{M}\;CuSo_4$. The electrophoretic mobility of the LDL oxidized in the presense of $5\;\mu\textrm{M}\;CuSo_4$ was higher than that of native LDL.

• Journal of Acupuncture Research
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• v.26 no.2
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• pp.41-48
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• 2009

Objectives : The conventional direct and indirect moxibustion therapies for prostate treatment could not been applied to the acupuncture point of $CV_1$(Conception Vessel Meridian 1, 會陰) because of its boring body region. The position of $CV_1$(會陰) is the back side of hard part between the anus and the genital organ. The conventional moxibustion methods have many troubles in operating to the acupuncture point of $CV_1$(會陰). In order to get rid of these problems, we have suggested the special heat generating terminal especially for prostate. The features of the special heat generating terminal for prostate are the low temperature infrared heater and the adhesive moxa-pad. These features are no burnt, no fiery and especially suitable for the point of $CV_1$(會陰). Methods: The heat generating terminal which is a part of the moxa-extract moxibustion cauterizer is composed of a PTC(Positive Temperature Coefficients) ceramic heater and the adhesive moxa-pad We had got the experimental demonstrations by the stimulating the acupuncture points which are $CV_1$(會陰), $BL_{28}$(Bladder Meridian 28, 膀胱兪), and $CV_3$(Conception Vessel Meridian 3, 中極) with the special heat generating terminal for the prostatitis and the benign prosthetic hypertrophy. And the stimulation level was 43$^{\circ}C$ infrared heat for one hour. The type of thermography is IRIS-5000. Results : With one subject suffering the prostatitis and another subject suffering the benign prosthetic hypertrophy, we cauterized the acupuncture points $CV_1$(會陰), $BL_{28}$(膀胱兪) and $CV_3$(中極) with the special heat generating terminal for prostate. We measured the temperature variations by the thermography before and after stimulations. Finally we estimated the tendency of temperature decreasing in the region of post-stroke urinary symptoms and the improvement of nocturnal enuresis after the stimulations. Conclusions : We suggest that the special heat generating terminal of moxa-extract moxibustion cauterizer proposed herein is effective for the treatment of prostate by NIH-CPSI and IPSS.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.28 no.2
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• pp.81-88
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• 2016

There are many types of national and international standards for low temperature tests depending on the products. This study conducted CFD analysis and estimation of the energy efficiency of the chamber both with and without a test object by considering variations of COP and specific volume according to temperature. The supply air was located in the upper area to compare the cooling performance for each location using various outlets in mixed ventilated conditions. For cases without the test object, the air temperature of the upper supply and center extract on the opposite side type chamber cooled faster than other areas by 4.3~29.8%. However, for cases with the test object, the object temperature of the upper supply and lower extract type chamber cooled faster than the other areas by 7.2~31.5%. The cooling efficiency of the air inside the chamber and the test object did not show the same pattern, which indicates the need to consider the cooling performance by not only the air but also the test object in the cryogenic chamber design for testing.

• Journal of the Korean Applied Science and Technology
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• v.27 no.1
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• pp.76-86
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• 2010

This study was conducted to evaluate the effects of dietary Korean red pine bark extract as an antibiotic replacements on cholesterol, fatty acids and the shelf-life of chicken meat. To accomplish this, chickens were fed the optimal level of red pine bark extract that was found to replace antibiotics in the diet of broilers. A total of 180 male broilers(Ross strain 308) were divided into three treated groups, T1(control group), T2(8 ppm of avilamycin) and T3(65 ppm of red pine bark extract per kg diet). The lipid content was reduced by 24.67% and 20.49% in T3 group, while the cholesterol level also decreased significantly in the T3 group by 20.49% and 20.55% when compared to the T1 and T2 groups, respectively. In addition, the saturated fatty acid level was lower in the T3 group than in the T1 and T2 groups, while the unsaturated fatty acid level of the T3 group was significantly higher than those of the other groups. The TBARS value of chicken thigh muscle containing its skin on the 7th day of low temperature storage was significantly lower by 23.86% and 21.17% in the T3 group than in the T1 and T2 groups, respectively. Evaluation of the color of the meat revealed that the $L^*$value (lightness) and $b^*$value(yellowness) were higher in the T3 group than in the T1 and the T2 groups, but that the pH was significantly lower in the T3. Based on the results of this study, the addition of 65 ppm red pine bark extract to the diet of broilers should improve their meat quality with respect to the lipid contents and shelf-life when compared to the addition of antibiotics.

• Preventive Nutrition and Food Science
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• v.12 no.4
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• pp.242-250
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• 2007

This study evaluated the storage stability of a variety of sauce products in the Korean market, determined primary quality indices for three typical products, and proposed functional relationships that are useful for determining shelf life at different temperatures. Most of the products examined were found to combine hurdles of low pH, low water activity, and the use of heat processing as methods for producing the required storage stability while maintaining the sensory quality of the products. For a meat extract solution produced for cold noodles (pH=4.3; $a_w=0.98$), the primary quality change determining shelf life was lipid oxidation, determined here by the TBA value. The primary quality index of a soybean paste seasoning mix (pH=4.0; $a_w=0.78$), which had a microbial load of 2.8 log (CFU/g), was a decrease in its pH. The primary quality index for a sandwich spread (pH=4.0; $a_w=0.88$) was changes in its surface color. The temperature dependence of changes in the primary quality indices can be described by the Arrhenius equation, which can estimate the shelf life at any arbitrary limit as a function of temperature. The activation energies for changes in the primary quality indices of the meat extract solution, the soybean paste seasoning, and the sandwich spread were 20.3, 27.2, and 43.5 kJ/mol, respectively.

• Korean Journal of Food Science and Technology
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• v.24 no.3
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• pp.199-203
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• 1992

This study was aimed to optimize the required condition for recovering the low soluble taste component obtained from sea tangle extract using ultrafiltration, and to investigate effects of membrane type, temperature, transmembrane pressure and flow rate respectively. We also compared relationship between the profile of permeate flux and the recovery yield of taste component under the selected optimal condition using ultrafiltration and diafiltration. Hydrophobic GR 51 PP membrane kept higher average permeate flux than hydrophilic FS membrane, and average permeate flux also had increasing tendency in relation to rising flow rate but it showed limit value of 3.7 l/min. Average permeate flux decreased as transmembrane pressure increased but it showed little change with rising temperature. Investigation upon average permeate flux, total dissolved solid and recovery yield of taste components using ultrafiltration and diafiltration resulted in relatively higher recovery yield in ultrafiltration. Compared ultrafiltration and diafiltration, average permeate flux was lower in ultrafiltration.

• Korean Journal of Microbiology
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• v.51 no.1
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• pp.75-80
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• 2015

Pseudoalteromonas donghaensis HJ51, isolated from the East Sea, has been reported as a novel strain to produce extracellular protease. Crude supernatant was used to determine optimal activity and optimal production conditions for the enzyme. It was found that the optimal temperature and pH of the protease were $40^{\circ}C$ and pH 7.5-10.5, respectively. The enzyme activity was kept to 88% at the pH 11. In metal requirement analysis, the enzyme exhibited the highest activity when 10 mM $Fe^{3+}$ was supplied. While supplementation of additional carbon sources used in study showed no positive effect on cell growth and enzyme activity, the addition of beef extract, tryptone, or casamino acids instead of peptone of PY-ASW containing 1% glucose increased enzyme production to 21, 7, 4%, respectively. Taken together these properties, the enzyme produced from P. donghaensis HJ51 can be applied to the industries that require protease activity under alkaline pH and low temperature.