• Microbiology and Biotechnology Letters
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• v.33 no.1
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• pp.1-8
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• 2005

Since Anton van Leeuwen­hoek first observed a surface-associated multicellular structure of bacterial cells in the 17th century, it has been shown to exhibit an ability to form a biofilm by numerous bacterial species. The biofilm formation is composed of distinct developmental stages, which include an attachment/adhesion of a single cell, a proliferation toward monolayered coverage, a propagation to aggregated microcolony, a maturation to 3-dimensional structure, and subsequently a local degradation. Investigation to identify the essential factors for bacterial biofilm formation has been performed via classical genetic approaches as well as recently developed technologies. The initial stage requires bacterial motility provided by a flagellum, and outermembrane components for surface signal interaction. Type IV-pilus and autoaggregation factors, e.g., type I-fimbriae or Ag43, are necessary to reach the stages of monolayer and micro colony. The mature biofilm is equipped with extracellular polymeric matrix and internal water-filled channels. This complex architecture can be achieved by differential expressions of several hundred genes, among which the most studied are the genes encoding exopolysaccharide biosyntheses and quorum-sensing regulatory components. The status of our knowledge for the biofilms found in humans and natural ecosystems is discussed in this minireview.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.6
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• pp.620-627
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• 2000

The purpose of this study is to investigate the clinical and histologic changes in distraction osteogenesis according to different distraction rates in the rat's tibia. Eighteen adult rats underwent open osteotomy and attachment of an external unilateral distraction device in the middle of left tibia. Latency was allowed for 7 days before distracton began. The distraction device was activated with varying distraction rates of 0.5mm, 1mm, 2mm and same rhythm of twice a day until 5mm length gain was achieved. The animals were sacrificed at post-distraction 4, 8 weeks to observe the bony healing states. At each group, clinical, radiographic and histologic studies were done. The results obtained from this study were as follows: 1. The 0.5mm group showed excellent osteogenesis than other groups. The new bone was formed by intramembranous bone formation mostly and endochondral bone formation partly. 2. The 1mm group showed delayed osteogenesis and incomplete bony healing at 8 weeks. 3. The 2mm group showed weak osteogenesis and fibrous union or nonunion at 8 weeks. From these results, it could be stated that distraction rate of 0.5mm per day was most useful in rat's tibia. The rate of 1mm showed delayed bony healing and needed more consolidation period. Distraction osteogenesis is a excellent clinical method for regenerating local bone deficiencies in limbs and craniofacial area. The more studies needed for the higher animals and human about distraction rates and other biomechanical factors on the basis of this study.

• Journal of Periodontal and Implant Science
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• v.33 no.2
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• pp.247-257
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• 2003

The purpose of this study was to evaluate the difference of subgingival bacterial compositions between periodontally healthy and diseased sites. Subgingival plaque samples were obtained from 100 sites in 20 untreated adult periodontitis patients(experimental group), and 100 sites in healthy individuals(contro1 group). Before sampling, probing pocket depth(PPD) and clinical level of attachment(CAL), Plaque Index(PI), and Sulcus Bleeding Index(SBI) were recorded for each sampled sites. Microbial samples were collected from the bases of gingival sulci or periodontal pockets with sterile curettes. The samples were examined under darkfield microscope(${\times}$400). At least 150 bacteria were evaluated and categorized on the basis of bacterial morphology and motility, i.e. cocci, non-motile rods, motile rods, and spirochetes. In control group, subgingival microbial flora consisted of 73.7% of cocci, 20.0% of non-motile rods, 4.3% of motile rods, and 2.0% of spirochetes. The microbial samples from experimental group consisted of 51.5% of cocci, 19.4% of non-motile rods, 17.6% of motile rods, and 11.6% of spirochetes. The proportion of cocci was higher in control group than in experimental group. Proportions of motile rods and spirochetes were higher in experimental group than in control group. The proportion of nonmotile rods in experimental group and control group was not significantly different. Sulcus Bleeding Index and Plaque Index showed high correlation with the bacterial composition. These findings suggests that examination of subgingival bacterial proportion may serve as more sensitive mirror of the local periodontal status than clinical parameters.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.65 no.6
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• pp.1083-1088
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• 2016

25.8kV GIS part generated by sensors to measure contact an inflow of noise depending on the extent of the measured discharge occurs often not easy. Partial discharge signal measurement sensor suitable for developing a more useful measurements at the scene to this, partial discharge waveform analysis developed a sensor, and to utilize forSensor on the development of the most important is VSWR decided to (voltage standing wave ratio) voltage standing-wave ratio less than 1.5 and decided less than at the full spectrum bands that are measured, this time Return loss, as measured value by absolute criteria 14.0 dB produced the sensor, designed to or more. UHF 1.5~0.5 GHz bandwidth spectrum to be measured in GIS. UHF bands were designed to be able to measure the best signal. Recently, 25.8kV GIS production company has been increasing variety of GIS were made open spacer in partial discharge in accordance with the not very easy to detect the signal. The sensor is designed height of four cm external spacer is attachment GIS in an influx of outside noise measurement, and be so manufactured as to facilitate the least we've done. Also, since partial discharge which occur can measure the frequency of the 170kV GIS external partial-discharge signals that occur at the scene of insulation applied to the spacer. Features, and also derived good results using global positioning. Also measured discharge point about sensors that are stable and the reliability of the development and local substation equipment failure occurring signal analysis through the discharge for the prevention of widely. There should be to believe that used.

• Journal of Periodontal and Implant Science
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• v.48 no.5
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• pp.326-333
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• 2018

Purpose: The present study investigated the outcomes of a newly-developed, simple, and practical nonsurgical treatment modality suitable for most forms of intrabony defects around failing dental implants using intrasulcular delivery of chlorhexidine solution and minocycline hydrochloride (HCl). Methods: Forty-five dental implants in 20 patients diagnosed with peri-implantitis were included. At baseline and the study endpoint, the probing pocket depth (PPD), clinical attachment level (CAL), and the presence of bleeding on probing (BOP) at 6 sites around each implant were recorded. The radiographic osseous defect morphology at the mesial or distal proximal aspect of each implant was classified as 1) narrow or wide and 2) shallow or deep. For a comparative analysis of bone changes according to the defect morphology, the distance from the implant shoulder to the most coronal bone-to-implant contact point (DIB) at the mesial and distal aspects of each implant was measured at baseline and the endpoint. Patients were scheduled to visit the clinic every 2-4 weeks for intrasulcular irrigation of chlorhexidine and delivery of minocycline HCl. Results: We observed statistically significant decreases in PPD, CAL, and BOP after treatment. At the endpoint, bone levels increased in all defects, regardless of the osseous morphology of the intrabony defect. The mean DIB change in deep defects was significantly greater than that in shallow defects. Although the mean bone gain in narrow defects was greater than in wide defects, the difference was not statistically significant. Conclusions: We propose that significant and sustainable improvements in both clinical and radiographic parameters can be expected when intrabony defects around dental implants are managed through a simple nonsurgical approach involving combined intrasulcular chlorhexidine irrigation and local delivery of minocycline HCl.

• Journal of Internet Computing and Services
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• v.5 no.1
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• pp.25-32
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• 2004

Mobile computing environment makes it possible for computing activities to be maintained during movement even if a user changes its network point of attachment. Mobile IP is a standard protocol designed to be used in such mobile computing environment. However, Mobile IP has a drawback to incur a lot of handoff delays and waste network resources, since CoA(Care of Address) registration packets need to go through a HA(Home Agent) first whenever a mobile node moves. To solve this long-standing problem, this paper proposes a new scheme that, for infra-domain movement, efficiently performs local handoff without notifying the HA Specifically, based on the notion of load balance, the proposed scheme allows every FA(Foreign Agent) in a domain to become the root FA(also known as domain FA) dynamically, thus distributing the registration task into many other foreign agents. The dynamic root assignment through load balancing ultimately leads to fast network response due to less frequent transmission of registration packets.

• Macromolecular Research
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• v.14 no.5
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• pp.565-572
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• 2006

Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly$(\varepsilon-caprolactone)$ (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.

• Journal of Periodontal and Implant Science
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• v.48 no.1
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• pp.47-59
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• 2018

Purpose: To evaluate the clinical and microbiological effects of the local use of egg yolk immunoglobulin against Porphyromonas gingivalis (anti-P.g. IgY) as an adjunct to scaling and root planing (SRP) in the treatment of moderate to severe chronic periodontitis. Methods: This was a randomized, placebo-controlled, double-blind trial involving 60 systematically healthy patients with moderate to severe chronic periodontitis. Subjects (n=20/group) were randomly assigned to receive SRP combined with subgingival irrigation of anti-P.g. IgY and anti-P.g. IgY mouthwash, subgingival irrigation of 0.2% chlorhexidine and 0.2% chlorhexidine mouthwash, or subgingival irrigation of placebo and placebo mouthwash for 4 weeks. Probing pocket depth, clinical attachment level, bleeding on probing, and the plaque index were evaluated at baseline and at 4 weeks. Subgingival plaque, gingival crevicular fluid, and saliva were simultaneously collected for microbiological analysis. Results: Our results showed that anti-P.g. IgY mouthwash was as effective as chlorhexidine at improving clinical parameters over a 4-week period. All the groups showed a significant reduction in levels of P.g. at 4 weeks. No significant difference was observed in the test group when compared to placebo regarding the reduction in the levels of P.g. Anti-P.g. IgY significantly suppressed the numbers of red complex bacteria (RCB) in subgingival plaque and saliva in comparison with placebo. No adverse effects were reported in any of the subjects. Conclusions: Within the limitations of the study, the present investigation showed that passive immunization with anti-P.g. IgY may prove to be effective in the treatment of chronic periodontitis due to its ability to improve clinical parameters and to reduce RCB. No significant differences were found between the anti-P.g. IgY and placebo groups in the reduction of P.g.

• Journal of the Korean association of regional geographers
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• v.10 no.2
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• pp.267-285
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• 2004

Globalization has been the buzzword during the past couple of decades, embroiling humankind into the inescapable maelstrom of homogenization. Some critics identify the globalization process undergoing in the realms of politics, economy, culture and ecology with Americanization which entails Neoconservatives' scheme to stretch out the hegemony of the United States. The top-down transcontinental movement, however, deems to confront localization or a reasoned resistance from the local driven by the spiritual attachment to places and, in that sense, regional identity. What is needed to cope with this complex circumstance of glocalization is to be acquainted with beth the global sense of the local and the localized sense of the global at once. For this, it seems indispensible to do justice to area studies. As far as American studies are concerned, this research field has so far been tainted by an overdose of politics, economics, business administration, law and sociology. Regional analysis which constitutes the reason d'etre of the discipline of geography has remained marginal to the political economic mainstream. It is the mounting concerns about America and enhanced research caliber that raises the regional geographical studies of America on the right track. Particularly, a number of articles have been published since the mid-1990s owing to the combined efforts of practitioners, institutions and periodicals. It is expected that systematic training of new generation of American specialists, advance in research infrastructure, and generous funding will stimulate geographical investigation of America in the coming future.

• Journal of the Korea institute for structural maintenance and inspection
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• v.15 no.2
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• pp.98-106
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• 2011

The deck of steel box girder bridges is composed of deck plate, longitudinal rib, and transverse ribs. The orthotropic steel decks have high possibility to fatigue damage due to numbers of welded connection part, the heavy contact loadings, and the increase of repeated loadings. Generally, the local stress by the repeated loadings of heavy vehicles causes the orthotropic steel deck bridge to fatigue cracks. The increase of traffic volume and heavy vehicle loadings are promoted the possibility of fatigue cracks. Thus, it is important to exactly evaluate the structural behavior of bridge considering the contact loading area of heavy vehicles and real load patterns of heavy trucks which have effects on the bridge. This study estimated the effect of contact area of design loads and real traffic vehicles through the finite element analysis considering the real loading conditions. The finite element analysis carried out 4 cases of loading patterns in the orthotropic steel deck bridge. Also, analysis estimated the influence of contact area of real truck loadings by the existence of diaphragm plate. The result of finite element analysis indicated that single tire loadings of real trucks occurred higher local stress than one of design loadings, and especially the deck plate got the most influence by the single tire loading. It was found that the diaphragm attachment at joint part of longitudinal ribs and transverse ribs had no effects on the improvement of structural performance against fatigue resistance in elastic analysis.