Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
권호 표지

Bone Morphogenic Protein-2 (BMP-2) Immobilized Biodegradable Scaffolds for Bone Tissue Engineering

  • Kim, Sung-Eun (Neuroscience Genome Research Center, The Catholic University of Korea) ;
  • Rha, Hyung-Kyun (Neuroscience Genome Research Center, The Catholic University of Korea) ;
  • Surendran, Sibin (Dept. of Orthopedic Surgery, Daejeon St. Mary's hospital, The Catholic University of Korea) ;
  • Han, Chang-Whan (Dept. of Orthopedic Surgery, Daejeon St. Mary's hospital, The Catholic University of Korea) ;
  • Lee, Sang-Cheon (Nanomaterials Application Division, Korea Institute of Ceramic Engineering and Technology) ;
  • Choi, Hyung-Woo (Nanomaterials Application Division, Korea Institute of Ceramic Engineering and Technology) ;
  • Choi, Yong-Woo (Dept. of Anesthesiology, St. Mary's hospital, The Catholic University of Korea) ;
  • Lee, Kweon-Haeng (Dept. of Pharmacology, The Catholic University of Korea) ;
  • Rhie, Jong-Won (Dept. of Plastic Surgery, Kangnam St. Mary's Hospital, The Catholic University of Korea) ;
  • Ahn, Sang-Tae (Dept. of Plastic Surgery, Kangnam St. Mary's Hospital, The Catholic University of Korea)
  • Published : 2006.10.31
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Abstract

Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly$(\varepsilon-caprolactone)$ (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.

Keywords

References

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