• Asian-Australasian Journal of Animal Sciences
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• v.25 no.1
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• pp.86-91
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• 2012

An in vitro rumen batch culture study was completed to compare effects of common grasses, leguminous shrubs and non-leguminous shrubs used for livestock grazing in Australia and Ghana on $CH_4$ production and fermentation characteristics. Grass species included Andropodon gayanus, Brachiaria ruziziensis and Pennisetum purpureum. Leguminous shrub species included Cajanus cajan, Cratylia argentea, Gliricidia sepium, Leucaena leucocephala and Stylosanthes guianensis and non-leguminous shrub species included Annona senegalensis, Moringa oleifera, Securinega virosa and Vitellaria paradoxa. Leaves were harvested, dried at $55^{\circ}C$ and ground through a 1 mm screen. Serum bottles containing 500 mg of forage, modified McDougall's buffer and rumen fluid were incubated under anaerobic conditions at $39^{\circ}C$ for 24 h. Samples of each forage type were removed after 0, 2, 6, 12 and 24 h of incubation for determination of cumulative gas production. Methane production, ammonia concentration and proportions of VFA were measured at 24 h. Concentration of aNDF (g/kg DM) ranged from 671 to 713 (grasses), 377 to 590 (leguminous shrubs) and 288 to 517 (non-leguminous shrubs). After 24 h of in vitro incubation, cumulative gas, $CH_4$ production, ammonia concentration, proportion of propionate in VFA and IVDMD differed (p<0.05) within each forage type. B. ruziziensis and G. sepium produced the highest cumulative gas, IVDMD, total VFA, proportion of propionate in VFA and the lowest A:P ratios within their forage types. Consequently, these two species produced moderate $CH_4$ emissions without compromising digestion. Grazing of these two species may be a strategy to reduce $CH_4$ emissions however further assessment in in vivo trials and at different stages of maturity is recommended.

• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.175-181
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• 2007

Parasites cause great economic loss in livestock in Korea, and can be categorized as either or indirect losses. In order to monitor the parasites from October 2005 to January 2006, 401 samples were randomly collected from cattle farms and examined the prevalence of gastro-intestinal (GI) parasites by using the sucrose flotation method and formalin-ether centrifugation method. The overall infection rate of GI parasites was about 43.4%. Of these parasites, nematodes were identified as Capillaria bovis (5.5%), Strongyloides papillosus (2.5%), Trichuris discolor (1.2%). Cestoda was observed only one species, Moniezia benedeni (2.2%). The rate of Eimeria spp was 39.2%. A total of five species of Eimeria was identified; among the species identified as above E bovis and E zuernii appeared with relatively higher infection rates. The combined infection of parasites was 35.2% in single species, 29.0% in double and 2.0% in triple infections. No cases of clinical symptoms were observed in this survey. The EPG levels of each species of parasites were very low in all infected cases.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.38-42
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• 1991

The investigation of the kinds and density of white grubs in relation to soil texture, forages, years after pasture establishment, and management methods in pasture were surveyed in Livestock Experiment Station, Suwon by digging 5 sites($100cm{\times}100cm$) in a month from April to October during 1985. The results obtained were summarized as follows; No difference was observed in white grub species divesity in relation to soil texture, forages, years after pasture establishment, and management method of pasture, but the abundance of each species was different from the environmental factors.1. On the soil texture; Miridiba kureana, Popillia uchidai, Holotrichia mmosa and Holotrichia diomphalia occurred in the clay loam field, whereas Anomala corpulenta and Anomala mfocuprea in the sandy loam. 2. On the forage; the density of all species except Popillia uchidai was higher in grass than legume. 3. On the years after pasture extablishment; both of the species diversity and abundance were increased 3 years after pasture establishment and maximized at the 5th year.4. On the management methods of pasture; species diversity and abundance were higher in meadows than pastures.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.305-313
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• 2020

Host shifting and host expansion of fungal plant pathogens increases the rate of emergence of new pathogens and the incidence of disease in various crops, which threaten global food security. Magnaporthe species cause serious disease in rice, namely rice blast disease, as well as in many alternative hosts, including wheat, barley, and millet. A severe outbreak of wheat blast due to Magnaporthe oryzae occurred recently in Bangladesh, after the fungus was introduced from South America, causing great loss of yield. This outbreak of wheat blast is of growing concern, because it might spread to adjacent wheat-producing areas. Therefore, it is important to understand the host range and population structure of M. oryzae and related species for determining the evolutionary relationships among Magnaporthe species and for managing blast disease in the field. Here, we collected isolates of M. oryzae and related species from various Poaceae species, including crops and weeds surrounding rice fields, in Korea and determined their phylogenetic relationships and host species specificity. Internal transcribed spacer-mediated phylogenetic analysis revealed that M. oryzae and related species are classified into four groups primarily including isolates from rice, crabgrass, millet and tall fescue. Based on pathogenicity assays, M. oryzae and related species can infect different Poaceae hosts and move among hosts, suggesting the potential for host shifting and host expansion in nature. These results provide important information on the diversification of M. oryzae and related species with a broad range of Poaceae as hosts in crop fields.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.2
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• pp.62-67
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• 1989

A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between $Lm{\times}Fa$, after 12 days embryos crossing between $Lm{\times}Lp$, Dg, and Ph.p, and after 16 days embryos crossing between $Lm{\times}Fp$. Both callus and shoot formation occurred on 6 % or less of the plated embryos of $Lm{\times}Fp$, Dg, and Ph.p. Embryoderived callus forming shoots have been obtained from 4 days old embryo crossing between $Lm{\times}Dg$ which has not successfully been done anywhere as far as we know. It means that hybrid plants of species without crossability can be obtained through the use of immatured embryo culture. Some of plated embryos developed directly shoots from embryos and the others shoot-forming callus. Cross between related species showed a high frequency of directly shoot formation from plated embryos and cross between remotely related species a high frequency of callus formation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.28-28
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• 1991

The investigation of the kinds and density of white grubs in relation to soil texture, forages, yearsafter pasture establishment, and management methods in pasture were surveyed in Livestock ExperimentStation, Suwon by digging 5 sites($100cm{\times}100cm$) in a month from April to October during 1985.The results obtained were summarized as follows; No difference was observed in white grub speciesdivesity in relation to soil texture, forages, years after pasture establishment, and management methodof pasture, but the abundance of each species was different from the environmental factors.1. On the soil texture; Miridiba kureana, Popillia uchidai, Holotrichia mmosa and Holotrichia diomphalia occurred in the clay loam field, whereas Anomala corpulenta and Anomala mfocuprea in the sandy loam. 2. On the forage; the density of all species except Popillia uchidai was higher in grass than legume. 3. On the years after pasture extablishment; both of the species diversity and abundance were increased 3 years after pasture establishment and maximized at the 5th year.4. On the management methods of pasture; species diversity and abundance were higher in meadows thanpastures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.298-309
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• 2018

This study examined the multi-functionality of an eco-pastoral system in alpine grassland and measured its economic value. The multi-functionality can be divided into three categories: direct-use value, indirect-use value, and heritage value. Direct-use value includes both extractive (forage and livestock production) and non-extractive (recreation and tourism) functions. Indirect-use value includes the functions of water conservation, soil erosion control, atmospheric control, landscape, livestock-manure management, and forest firebreaks. The heritage value includes the function of species diversity. The results showed that the annual value for 1 hectare of the eco-pastoral system in alpine grassland's direct use was estimated to be 21,090,874 Korean won; the indirect-use value was 15,562,203 won when the landscape in grassland system, and 16,018,224 won when the landscape comprised in silvopastoral system. The value of the species diversity in heritage terms ranged from 767,273 to 1,578,845 won, depending on whether it included any endangered species. The total value of multi-functionality of the eco-pastoral system in alpine grassland was estimated to be a minimum of 37,420,350 won/ha and a maximum of 38,687,942 won/ha. The results of this study can provide useful insights for the eco-pastoral system in alpine grassland policies in Republic of Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.2
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• pp.71-77
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• 1986

The field experiment was conducted to evaluate the effect of herbicide treatment (Banvel: 100, 200, U-46: 150, 300, Hedonal: 150, 300, Simazin: 100, 200g/10 a) on change in the botanical composition and dry matter productivity of ladino clover (Trifolium repens L.) dominated pasture mixtures. The experiment was laid down as a randomized block design with 4 replications at experimental field of Livestock Experiment Station in Suweon from 1983 to 1985. The results obtained are summarized as follows: 1. Against ladino clover in mixie grass-clover swards Banvel, U-46 and Hedonal gave good control. The percentage of ladino clover under herbicide tratment decreased to about 1-2%(Banvel), 11-18%(U-46) and 22-31%(Hedonal), respectively, while it increased to 71% in untreated pastures. However, in the second year this trend stated to reverse and occurred clover dominance again in U-46 and Hedonal application, while those remained approximately constant until end of the years under Banvel treatment. Simazin is to be not recommended. 2. The best time for herbicide treatment was found to be late summer before autumn sown 20-25 days. When it applied in early summer weed infestigation by Digitaria spp., Echinochloa spp. and other species was a severe problem. 3. Emergence and early development of introduced pastures were less satisfactory, if it oversown immediatley after herbicide treatments due to its phytotoxical damage. Residual chemicals remained about 7-10 days in topsoils. Perennial ryegrass and orchardgrass were slightly less tolerant than the other species. 4. In dry matter, taken as average of three year results, Banvel applied pastures produced the remarkably high yield of 1023 kg/10 a, which is as much as 44% higher than that of untreated plot. Dry matter yields under U-46 and Hedonal treatment were 842 and 811 kg/10 a, respectively. 5. Weender components and net energy concentration were affected by change in the botanical composition. Crude protein and NEL value were slightly higher in clover dominance than those in herbicide treatments. Total yields of net energy lactation, however, were the highest in Banvel application with 5401 MJ and the lowest in untreatment with 3889 MJ-NEL/10 a DM.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.3
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• pp.143-150
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• 2005

These experiment was conducted to determine the effect of nitrogen fertilizer levels and mixture small grains on productivity and quality of spring forage rape at south region of Korea(Mokpo). The experiment was arranged in a split plot design with three replications. The main plots consisted of three different levels of nitrogen fertilizer(100, 150 and 200 kg/ha). The sub plots consisted of five kinds of mixed small grain species(Rye, Oat, Barley, Italian ryegrass, and Rape monoculture). The results obtained are summarized as follow. Dry matter(DM) content of Rye + Rape and Ba.ley + Rape mixtures were increased by $2\~3\%$ compared to Rape monoculture. The high level of nitrogen application increased the fresh matter yield, and yield of Rye + Rape and Rape monoculture were higher than that of others. Dry matter yield of Rye + Rape mixture and Rape monoculture with 200kg/ha of nitrogen application were higher by 9,449 and 9,227 kg/ha, respectively. The crude protein(CP) content of rape was high as $18.6\%$ while average CP content was $16\%$. The average total digestible nutrient(TDN) content showed high as $70\%$. It is suggested the Rye + Rape mixture or Rape monoculture would recommendable for spring usage of rape at south region of Korea.