• Toxicological Research
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• 제12권2호
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• pp.213-221
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• 1996

In order to develop a suitable secondary renal disease model and diagnostic markers of renal disease in the rat, the change of PIIIP (aminoterminal procollagen III peptide) in serum and hydroxyproline levels in the renal tissue that reflect the synthesis of extracellular matrix (ECM) during development of experimental renal diseases were observed. Two types of experimental primary diseases, diabetes mellitus administrated by streptozotocin (STZ, 75 mg/kg, i.p.) and liver cirrhosis produced by bile duct ligation/scission (BDL/s) operation, were induced. The hydroxyproline level increased according to the high PIIIP and NCl(carboxyterminal procollagen IV peptide) in Western blot analysis as early as 1 week in the STZ treated-rat kidney. Increased renal ECM was observed at 15 weeks in STZ and BDL/s model under the microscopic examination. High PAS positive reaction was found in capillary basement membrane in STZ treated-rats and mesangium in BDL/s operated rats at this time, showing the histological characteristics of diabetic nephropathy and cirrhotic glomerulonephritis in human, respectively. Such secondary renal failure were supported by additional tests including urinalysis and renal function test. The serum PIIIP detected by ELISA was a useful parameter to estimate synthesis rate of renal ECM during development of renal disease without extrarenal fibrosis i.e. liver cirrhosis in rats. This study is proposed that STZ treatment or BDL/s operation may be a suitable experimental animal model for the induction and development of chronic secondary renal diseases. Morover, it was found that hydroxyproline level in renal tissues was a good parameter of the change of renal ECM at the early stage of the diseases without apparent histological changes. Especially, serum PIIIP could be a choice as a diagnostic or prognostic marker during the development of renal diseases in rats.

• 대한암한의학회지
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• 제24권1호
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• pp.19-28
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• 2019

Objective : The purpose of this study is to report and observe effects of Integrative Cancer Treatment (ICT) on metastatic breast cancer patient. Method : A left breast cancer patient diagnosed with recurrence on liver and bones April 2018. The patient had received paclitaxel chemotherapy for two months and discontinued it because of tumor progression and side effects. The patient has been treated with ICT since March 2018 and has been taking capecitabine since October 2018. The clinical outcomes were measured by computed tomography, laboratory findings including tumor markers (CEA, CA15-3). The clinical outcomes were measured by computed tomography, laboratory findings including tumor markers (CEA, CA15-3), liver function test (AST, ALT), Eastern Cooperative Oncology Group performance status (ECOG PS), and numeric rating scales (NRS). Results : After the ICT, tumor size was partially decreased accompanying by reducing the levels of tumor markers. Major clinical symptoms induced by paclitaxel chemotherapy were improved. There were no severe adverse events induced by ICT based on National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) version 5.0. Conclusion : This case suggests that ICT may have synergistic effect for the treatment of metastatic breast cancer patient in conjunction with capecitabine.

• Journal of Preventive Medicine and Public Health
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• 제28권3호
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• pp.543-550
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• 1995

이 연구는 대구시 소재 일개 검진기관에서 1992년도에 일반건강진단을 받은 근로자를 대상으로 하여 사무직 근로자와 생산직 근로자간의 차이가 선별검사 결과에서 있는지를 알기 위해 시행되었다. 대상자는 남자 6,597명과 여자 3,610명으로 총 10.2명이 선택되었다. 남녀별로 나누어 각 선별검사 항목별로 단일분석을 시행한 후 유의한 변수를 골라 지수 회귀분석을 이용하여 사무직종에 대한 생산직을 비교하였다. 청력검사 항목은 남녀 모두에서 사무직보다 생산직에서 이상률이 더 컸다. 혈구용적치와 고혈압 항목은 조정변수를 교정한 후에도 남녀모두 직종간의 차이가 없었다. 간기능과 요단백 항목은 남자에서 사무직의 이상률이 생산직보다 더 높은 것으로 나타났다. 시력항목은 여자에서만 직종간의 차이를 보였으며 사무직에서 시력교정의 필요성이 더 켰다. 이상의 결과는 사무직종과 생산직종간에 일반건강진단의 검사 결과에서 차이가 있으며 원인규명을 위한 연구가 필요함을 보여준다.

• Journal of Ginseng Research
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• 제36권2호
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• pp.176-189
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• 2012

This study examined the effects of Korean red ginseng (KRG) on obese women and aimed to confirm that the effects of KRG on obesity differ dependently on a gene. Fifty obese women were recruited and randomized to receive KRG (n=24) or placebo (n=26) for 8 wk. Measurements of blood pressure, height, weight, waist circumference, waist-hip ratio (WHR), total fat mass, percentage of body fat, resting metabolic rate, basal body temperature, and daily food intake (FI), blood test (serum lipid, liver and renal function), Korean version of obesity-related quality of life scale (KOQOL), and a gene examination were performed. Comparisons of subjects before and after the administration of KRG revealed significant improvements of obesity in terms of weight, body mass index (BMI), WHR, FI, and KOQOL. However, in the comparison between KRG group and placebo group, only KOQOL was significantly different. KRG displayed significant efficacy on BMI and KOQOL in the CT genotype of the G protein beta 3 gene, but not in the CC genotype, on blood sugar test in the Trp64/Arg genotype of the beta 3 adrenergic receptor gene, but not in Trp64/Trp genotype, on KOQOL in the DD genotype of the angiotensin I converting enzyme gene, but not in the ID and DD genotypes. The effects of KRG on obesity were confirmed to some extent. However, a distinct effect compared to placebo was not confirmed. KRG is more effective for improving the secondary issues of the quality of life derived from obesity rather than having direct effects on the obesity-related anthropometric assessment and blood test indices.

• 한국학교보건학회지
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• 제31권3호
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• pp.178-185
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• 2018

Purpose: This study aims to examine the effects of auriculotherapy on reducing signs of dry eye syndrome. Methods: This study is based on a pretest-posttest nonequivalent control group design. The participants who scored higher than 13 on the Ocular Surface Disease Index (OSDI) were divided randomly into two groups, the experimental group (n=45) and the control group (n=44). Auriculotherapy was performed by researchers for 2 weeks. For the experimental group, seed stickers were applied to the eye, liver, shenmen, and tubercle point of their ears. The pretest and the posttest included the same questionnaires of the OSDI as well as the Visual Function Questionnaire (NEI-VFQ25) and the eye fatigue. Data were analyzed by descriptive statistics, $x^2$ test, t-test and paired t-test, using SAS 20.0 program. Results: The difference in the OSDI's scores showed a significant decrease between the pretest and the posttest (t=-4.12, p<.001) in the experimental group. No significant difference was found in the NEI-VFQ25 (t=-0.10, p=.921, t=-0.91 p=.374). However, the experimental group showed a significant decrease in eye fatigue (t=5.78, p<.001), whereas the control group showed no difference (t=1.38, p=.18). Conclusion: Auriculotherapy can be used effectively on relieving the symptoms of dry eye syndrome and eye fatigue. However, the quality of life related to eyes was not improved by auriculotherapy.

• 대한약침학회지
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• 제6권1호
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• pp.76-94
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• 2003

Objectives : The purpose of this study was to investigate Acute$\cdot$Subacute Toxicity and Anti-cancer Effect of K-Herbal-acupuncture in mice and rats. Methods : Balb/c mice were injected intraperitoneally with K-herbal-acupuncture for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were injected intraperitoneally with K-herbal-acupuncture for subacute toxicity test. K-Herbal-acupuncture was injected on abdomen of mice with S-180 cancer cell line. Result : 1. $LD_{50}$ of K-Herbal-acupuncture was limited $4{\times}10^{-3}$ml/kg~$2{\times}10^{-3}$ml/kg by the test. 2. In acute toxicity test, all of mice were down to the moving reflex, but the weight of mice was increased in treatment group, compared with the normal group. (P<0.05) 3. In acute toxicity test of serum biochemical values of mice, glucose was increased in treatment II group, total cholesterol was increased both treatments.(P<0.05) 4. In subacute toxicity test, the clinical signs of toxication was down to the moving reflex, but it is not severe like acute toxicity test, and observed weight loss at the treatments. 5. In subacute toxicity test, liver weight was decreased compared with the normal group. (P<0.05) 6. In subacute toxicity test of complete blood count test (CBC) of rat, HCT was decreased in treatments, compared with the normal group.(P<0.05) 7. In subacute toxicity test of serum biochemical values of rat, uric acid and triglyceride were decreased, and glucose was increased in treatment groups compared with the control group. (P<0.05) 8. Median survival time was increased about $45\%$ in treatment groups compared with the control group.(P<0.05) 9. Natural killer cell activity was increased in B16F10 lung cancer model, but it was not in sarcoma-180 abdomen cancer. 10. In interleukin-2 productivity test, treatment groups didn't show significant change in lung cancer and abdomen cancer, compared with the normal group.(P<0.005) 11. In making an examination of metastatic cancer with the naked eye, melanoma metastasized in the Lung of C57BL/6 mice. The treated group showed more Melanoma than the control in the numbers and volume. Conclusion : According to the result, K-herbal-acupuncture need further study to know the function and effect in cancer.

• 한국축산식품학회:학술대회논문집
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• 한국축산식품학회 1998년도 정기총회 및 제21차 춘계학술발표회
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• pp.37-54
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• 1998

건국대학교본 연구는 쑥의 추출물을 이용해 생체내에서 에탄올의 분해효능, 간기능 및 Cd 독성 저하작용에 미치는 효과를 알아보기 위하여 실시하였다. 본 실험에서 에탄올 분해효능은 Rat 1kg 당 25% 에탄을 5g을 경구투여한 후 쑥추출물의 카테킨 농도를 측정하여 이를 기준으로 일일 사료섭취량의 2% 원료수준으로 각각 미정맥주사를 하였다. 에탄올만 경구투여한 대조군과 에탄올을 경구투여한 후 쑥추출물을 미정맥주사한 실험군은 시간별(0, 1, 2, 3시간)로 미정맥에서 혈액을 채취해 3000rpm에서 10분간 원심분리하여 혈장을 분리시킨 후 GC(Gas Chromatography)로 혈중 에탄올 농도를 측정하였다. 또한 같은 방법으로 시간만 달리해 (0시간, 5시간) 혈장을 취해 GOT, GPT의 활성치를 측정하여 간손상정도를 측정하였다. 간기능에 미치는 효과는 간실질세포를 기본배지에 배양한 대조군과 쑥추출물을 배지의 1% 및 2% 원료수준으로 기본배지에 첨가하여 간실질세포를 배양한 실험군으로 나누어 36시간 배양한 후 현미경으로 형태를 관찰하고 생존 세포수와 GOT, GPT의 활성치를 함께 측정하였다. Cd 독성 저하작용은 4주령의 Sprague-Dawley 수컷 흰쥐를 구입하여 대조군(CON,0.85% saline 10ml/kg B.W.)과 Cd단독 투여군(CD, 0.4mg $CdCl_2/kg$ B.W.), Cd과 쑥추출물을 복강내 투여한 군(IP, 쑥 추출물은 일일사료섭취량의 1% 수준) 그리고 쑥추출물을 구강내 투여한 군(PO, 쑥 추출물은 일일사료섭취량의 1%수준)으로 분류하여 4주간 사육하였다. 사육 후 각종 장기 및 혈액을 채취하여 장기에서의 GSH 농도, GSH-Px 및 GR의 활성과 혈장 GOT, GPT 활성을 측정하였고 더불어 각 조직에서 Cd축적량을 조사하였다. 실험결과를 요약하면 다음과 같다. (1) 쑥추출물의 카테킨의 함량을 Iwasa와 Torri의 방법을 변형하여 측정한 결과 네 가지의 추출물중에서 카테킨의 함량은 쑥의 열수추출물이 $8{\sim}10mg/100g$으로 가장 많았으며 쑥의 에탄올 추출물은 $3{\sim}4mg/100g$인 것으로 나타났다. (2) 쑥 추출물을 HPLC를 이용해 주요 카테킨의 성분을 비교한 결과 쑥의 열수추출물 및 에탄올추출물은 (-)-Epigallocatechin (EGC)의 함량이 가장 높았다. (3) 시간에 따른 혈중 에탄올 농도를 GC로 측정한 결과 각각 에탄올을 투여한지 1시간 후의 에탄올 농도에서는 CON-E의 경우가 가장 높은 것으로 나타났고 MDW-E, MOH-E군은 CON-E에 비해 유의하게(p<0.05) 감소하는 것을 알 수 있었다. (4) 간실질세포 배양 36시간 후 배지를 걷어내고 밑면에 모인 세포를 모아 현미경으로 수를 측정하여 평균을 낸 결과는 2% MDW-L가 가장 많았으며 2% MOH-L을 제외하고는 CON-L의 세포수가 가장 적었다. 또한 배양 후 취한 세포내의 GOT, GPT의 활성치는 실험군간의 유의한 차이는 없었다. (6) 간 조직중의 GSH 함량수준은 쑥추출물 투여군들(IP, PO)이 CD군에 비해 유의적으로 증가(p<0.05)하여 대조군과 같은 정상수준으로 되었다. (7) 간 신장에서의 GSH-Px 활성은 저농도의 Cd에 의해서는 영향을 받지 않은 것으로 나타났다. (8) 혈장 GOT활성은 IP군이 대조군에 비해 증가하였으나 CD군에 비해 유의적으로 감소(p<0.05)하였다. 이상의 결과에서 쑥추출물이 체내에서 에탄올 분해를 촉진시키고 간기능을 개선시킴은 물론 GSH농도의 정상회복과 GR의 증가 및 혈장에서의 GOT활성의 감소 등으로 보아 Cd의 간독성도 감소시키는 작용이 있는 것으로 사료되었다.

• 대한한의학회지
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• 제37권1호
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• pp.34-40
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• 2016

Objectives: To observe safety and efficacy of herbal medicine for psoriasis patients. Methods: We did retrospective chart review for 107 psoriasis patients who had been treated by herbal medicine for 2 months, and we analyzed the liver function test (LFT) results and Psoriasis Area and Severity Index (PASI) scores of before and after the treatment. Results: The mean LFT and the number of patients with abnormal LFT decreased after the treatments. More than 95% of patients with normal LFT maintained normal results at after the treatment, and only 2.1% and 4.6% patients with normal LFT showed abnormal Glutamic Oxaloacetic Transaminase (GOT) and Glutamic Pyruvic Transaminase (GPT) results at after the treatment. Also, both mean PASI scores for all patients and patients with abnormal LFT before the treatment decreased significantly. Conclusions: This study suggest that herbal medicine may be safe and effective treatment for psoriasis.

• 대한약침학회지
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• 제7권3호
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• pp.123-129
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• 2004

Nowadays Asthma is considered to be an inflamatory disease characterized by airway hyperresponsiveness and pulmonary eosinophilia. Hominis Placenta is the dried placenta of a healthy women. It has correspondence to the meridians of lung and kindey. Hominis Placenta acupuncture therapy has effect on invigoration of vital energy nourishing blood and tonifying the essence. It can be applied to the disease as Asthma, pulmonary tuberculosis, chronic hepatitis, liver cirrhosis, degenerative change and cerebrovascular disease. We treated two patients of Asthma with CVA by Homins Placenta Aqua-Acupuncture. The effect of Homins Placenta Aqua-Acupuncture was assessed by analyzing the pulmonary function test(PFT) and Quality of Life Questionnaire for adult Korean Asthmatics(QLQAKA) in patients before and after treatment. Total score was increased. The patients are satisfied our treatment. But further research concerning this is still necessary.

• 대한간호
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• 제12권5호통권67호
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• pp.83-92
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• 1973

1972년 1월부터 1972년 12월말까지 충남대학교 의과대학 부속병원에서 위 수술을 받은 환자 66명을 대상으로 여러 가지 통계 결과 1. 성별 분포에선 남자가 51명 여자가 15명으로 약 3.4:1의 비율을 나타냈다. 2. 연령별 분포에선 $69.7\%$가 40세 이상에서 나타났다. 3. Chief complain은 Indigestion이었다. 4. 증상의 평균 연한은 1년 전후의 Chronic symptom을 경험했다. 5. Vital sign의 변화는 대부분이 별로 나타나지 않았다. 6. 수술 전 수혈은 1000cc 이내로 환자의 $78\%$에 필요했었다. 7. 수술 전의 Hb은 정상보다 낮은 율은 $45.4\%$였고 정상은 $54.6\%$였다. 8. 수술 전의 W.B.C는 정상보다 낮은 율은 $10.6\%$, 정상이 $42.4\%$, 정상보다 높은 율이 $47\%$였다. 9. 수술 전의 Ht치는 정상보다 낮은 율은 $60.6\%$, 정상은 $36.4\%$ 정상보다 높은 율은 $3\%$였다. 10. 수술 전 위액검사 결과는 Cancer인 경우는 Free acid와 Total acid가 모두 거의 정상보다 낮았고 Ulcer인 경우 대부분 높았다. 11. Liver function test에서는 표에서 나타난 대로 많은 변화는 없었다. 12. 병소 부위는 Cancer의 경우 $51.8\%$에서 Pylorus부위에 왔었고 Ulcer의 경우 $62.4\%$에서 Lesser curvature (ciratricial region포함)에 왔었다. 13. 수술방법은 $77.7\%$가 Subtotal gastrectomy였었다. 14. 위 수술을 위한 입원 일자는 $60\%$ 이상에서 $11\~15$일간의 평균을 보였었다.