• Journal of Nutrition and Health
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• 제39권3호
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• pp.219-224
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• 2006

This study was performed to determine the effect of soy isoflavones on brain development and function in rats. Forty Sprague-Dawley male rats were provided diets containing different levels of soy isoflavones for 6 weeks; 0 ppm (control), 50 ppm (low isoflavone intake; LI), 250 ppm (medium isoflavone intake; MI) and 500 ppm (high isoflavone intake; HI). Learning ability was evaluated by a Y-shaped water maze and the activity of acetylcholinesterase in brain was assayed after decapitation. Food intake and body weights as well as weights of brain, liver, spleen, heart and kidney showed no significant difference among the four groups, which means 500 ppm of isoflavones is safe. In the water maze test, the frequency of error counted when rats entered one end of the alley without platform was significantly lower in the HI group than in the control group, and the escape latency as swim time taken to escape on the hidden platform was significantly shorter in the HI group than in the LI and control groups. The activity of acetylcholinesterase of the brain was significantly higher in the HI and MI groups than in the control group. Therefore, the results indicate that isoflavones may improve the cognitive function without adverse effects.

• 대한간호학회지
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• 제37권3호
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• pp.324-333
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• 2007

Purpose: The purpose of this study was to develop and evaluate a quality of life scale for Korean patients with cancer (C-QOL). Methods: The C-QOL was developed and validated as follows, item generation, pilot study, and psychometric tests. A total of 337 patients diagnosed with stomach, liver, lung, colon, breast, or cervix cancer were recruited. The patients were asked to complete the preliminary questionnaire comprising the content-validated items, the SF-36, and the ECOG performance status. The obtained data was analyzed using descriptive statistics, factor analysis, multidimensional scaling (MDS), multitrait/multi-item matrix, ANOVA, t-test, and Cronbach's alpha. Results: Preliminarily twenty-six items were generated through content validity and a pilot study. Factor analysis and MDS extracted a total of 21 items with a 5-point Likert-type scale (C-QOL). The C-QOL included five subscales: physical status (6 items), emotional status (6 items), social function (3 items), concern status (2 items), and coping function (4 items). The C-QOL established content validity, construct validity, item convergent and discriminant validity, known-groups validity, reliability, and sensitivity. Conclusion: The Newly developed C-QOL is an easily applicable instrument which established psychometric properties and reflected Korean culture. It is recommended for further study to examine the responsiveness of the C-QOL using a longitudinal research design.

• 동의생리병리학회지
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• 제20권3호
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• pp.663-669
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• 2006

This study was done to investigate the effect of Ganyangsanghang-bang(GYSHB) on hypertension. After administering GYSHB extract to SHR for 5 weeks, changes in blood pressure, pulse rate, aldosterone and catecholamine levels in plasma were examined, and immunohistochemical changes were observed, and liver function test was done. The following results were obtained; blood presure decreased significantly as well as levels of aldosterone and norepinephrine in SHR. But levels of dopamine were unaffected. No capillary vessel dilation in adrenal cortex was observed. Safety against hepatic toxicity was showed. These results support a role for GYSHB might be usefully applied in treatment of hypertension.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1993년도 춘계학술대회
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• pp.94-97
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• 1993

퍼지규칙에 기반을 둔 간기능검사 자동 분석 시스템을 제안하였다. 전문의의 지식과 경험을 수리화한 소속함수를 설정한 후 4가지의 간질환이 있는 118명의 환자를 대상으로 측정된 6종목의 간기능검사 결과에 적용하여 80%의 높은 적중율을 얻었다. 질환별 적중향상은 전문의가 판정한 때 예상되는 것과 일치하였다. 본 연구에서 제안하는 시스템은 유사한 상황의 모든 진단시스템에 동일하게 적용할 수 있다.

• 대한인간공학회지
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• 제29권6호
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• pp.897-905
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• 2010

The purpose of this study is to identify relationships between safety and health indices in a shipbuilding industry. From the results of periodical health examination, job stress test, and health questionnaire for musculoskeletal disorders, the author stratified the 704 shipbuilding workers into categories of smoking, drinking frequency, the amount of exercise per week, obesity, blood pressure, diabetic symptoms, liver function, audibility, job stress, fatigued condition, musculoskeletal symptom, and accident. The results show that occupational injuries are highly related to smoking, drinking frequency, auditory capacity, fatigued condition, and musculoskeletal symptoms. These results can be used to develop more effective accidental occupational injury prevention programs for shipbuilding industries.

• 동의생리병리학회지
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• 제20권1호
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• pp.131-137
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• 2006

This study was done to investigate the effect of Gamisamool-tang(GMSMT) on hypertension. After administering GMSMT extract to SHR for 5 weeks, changes in blood pressure, pulse rate, aldosterone and catecholamine levels in plasma were examined, and immunohistochemical changes were observed, and liver function test was done. The following results were obtained; blood presure decreased significantly as well as levels of aldosterone, norepinephrine and epinephrine in SHR, But levels of dopamine were unaffected. No capillary vessel dilation in adrenal cortex was observed. Safety against hepatic toxicity was showed. These results support a role for GMSMT might be usefully applied in treatment of hypertension.

• 대한약침학회지
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• 제7권1호
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• pp.37-51
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• 2004

Objective : Recently scolopendrid aqua-acupuncture has been a good effect on pain control but it has not been known about clinical safety. The purpose of this study was to investigate acute toxicity of scolopendrid aqua-acupuncture. Method : In order to prove the clinical safety of scolopendrid aqua-acupuncture, We have observed a bacteriological examination and clinical pathology test after scolopendrid aqua-acupuncture treatment. Balb/c mice were injected intravenous with Scolopendrid aquaacupuncture treatment for $LD_{50}$ and acute toxicity test. We analyzed physical reaction(side effect)and clinical pathology test before and after Scolopendrid aqua-acupuncture treatment of mice and 20 patients suffering from pain, who admitted department of Acupunture and Moxibustion, College of Oriental Medicine, Won-Kwang University Kwangju hospital. Results : In the Blood agar plate and Nutrient agar plate, a bacteriological examination did not show a bacillus. In acute $LD_{50}$ toxicity test, there was no mortality thus unable to attain the value. Examining the toxic response in the acute toxicity test, there was no sign of toxication. In acute toxic test, running biochemical serum test couldn't yield any differences between the control and experiment groups. In the 20 patients treated with Scolopendrid aqua-acupuncture, hematologic test did not show remarkable change. In the 20 patients treated with Scolopendrid aqua-acupuncture, Liver function test(AST, ALT, ALP) showed a slight decrease on the contrary, and abnormal rate showed a decrease of 5.0% compared with previous study. Reanl function test(BUN, Cr) and abnormal rate showed a decrease of 5.0% compared with previous study. In the 20 patients treated with Scolopendrid aqua-acupuncture, Electrolyte were normal range before and after treatment. In the Urine analysis of 20 patients, Leukocyte, Protein, Glucose, Keton, Bilirubin, U-bilinogen were not detected before and after Scolopendrid aqua-acupuncture treatment, and the rest almost made no difference.

• 대한한방내과학회지
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• 제32권2호
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• pp.153-169
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• 2011

Objectives : This study was performed to investigate the anti-fibrogenic effect and the effect on cell growth and apoptosis in YJCGT, YJCGT YSO and YJCGT YSCO on thioacetamide-induced rat liver tissue and the immortalized human hepatic cell line LX2. Materials and Methods : LX2 cells were treated with various concentrations (0, 50, 150, 300 ug/ml) of YJCGT, Y+YSO, and Y+YSCO extract for 24, 48 and 72 hours. After the treatment, cell viability was measured by using MTT assay. Caspase inhibitor assay, and cell viability were determined by a colorimetric assay with PMS/MTS solution. Rat liver fibrosis was induced by intraperitoneal thioacetamide injection 150 mg/kg 3 times a week for 5 weeks. After the treatment, body weight, liver & spleen weights, liver function test, the complete blood cell count and the change of portal pressure were studied. After YJCGT, Y+YSO, and Y+YSCO treatment, percentages of collagen in thioacetamide-induced rat liver tissue were measured. Results : The viability of the LX2 cell decreased in a dose- and time-dependent manner. Exposure of LX2 cells to YJCGT, YJCGT+YSO and YJCGT+YSCO induced caspase-3 activation, but co-treatment of YJCGT, YJCGT+YSO and YJCGT+YSCO with the pan-caspase inhibitor Z-VAD-FMK, and the caspase-3 inhibitor Z-DEVE-FMK, blocked apoptosis. There was no difference in rat body weight between the thioacetamide only group and the YJCGT, YJCGT+YSO and YJCGT+YSCO groups. In the YJCGT, YJCGT YSO and YJCGT YSCO groups, the serum level of GPT significantly went down compared with the thioacetamide only group. In the YJCGT, Y+YSO, Y+YSCO groups, white blood cell elevated by thioacetamide injection decreased but RBC, Hgb, and Hct increased. In the Y+YSO group, the portal pressure elevated by thioacetamide injection significantly decreased. In the histological finding, thioacetamide injections caused severe fibrosis, but YJCGT, Y+YSO, and Y+YSCO treatment significantly reduced the amounts of hepatic collagens. Conclusions : YJCGT, Y+YSO, and Y+YSCO inhibit the growth of LX2 cells by inducing apoptosis through caspase activity. YJCGT, Y+YSO, and Y+YSCO have beneficial effects on the treatment of cirrhotic patients as well as patients with chronic hepatitis.

• 한국식품영양과학회지
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• 제43권8호
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• pp.1139-1147
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• 2014

본 연구는 알코올성 간 손상을 일으킨 흰쥐에게 오미자추출물이 혈중 알코올 분해율과 간 기능 보호에 미치는 영향을 구명하기 위하여 단회성 급성주정중독시험과 장기 주정투여시험을 각각 수행하였다. 급성주정중독시험은 흰쥐에게 급성주정중독량과 오미자추출물을 투여한 후 60분, 90분, 150분, 240분, 300분, 360분의 혈중 에탄올 농도를 측정하여 경시적인 알코올 분해율을 측정하였다. 장기 주정투여시험은 전반기(4주)는 급성주정중독량(22,23)을 참고하여 1/2용량의 시험주정(Chivas Regal, 40%)을 투여하였고, 후반기(4주)는 시험주정과 함께 물과 오미자추출물(150 mg, 1.0 mL)을 각각 투여하였다. 급성주정중독량시험에서 주정투여 후 90분의 혈중 알코올 농도가 가장 높았고, 주정투여 후 알코올의 기준 농도 대비 360분의 혈중 알코올 분해율은 주정단독투여군(A) 81.4%, 오미자투여군(B) 96.1%, 주정+물투여군(C) 85.3%로 오미자투여군이 다른 두 주정투여군보다 평균 13% 더 높았다. 장기 주정투여시험에서 간장무게는 두 주정투여군(A, B)이 일반대조군(C)보다 통계적으로 무거웠다(P<0.05). 혈청과 간 조직의 중성지방(TG) 함량은 일반대조군이 두 주정투여군보다 통계적으로 유의하게 낮았다(P<0.05). 간 조직의 TG 함량은 오미자투여군(B)이 주정대조군(A)에 비해 26.5% 낮았다. 혈청 중 총 콜레스테롤(TC) 함량은 실험군 간에 차이가 없었고, 간 조직의 TC 함량은 B군이 A군보다 18.8% 낮았다. 주정과 오미자추출물을 투여한 후 측정한 ${\gamma}$-GT, AST 및 ALT 활성은 오미자추출물군(B)이 주정대조군(A)보다 각각 26%, 12%, 19% 감소하였다. 혈액학치(CBC)는 혈소판을 제외하고는 실험군 간에 통계적인 차이가 있었다(P<0.05). 간세포 조직의 Steatosis score는 주정대조군이 3점인 반면 오미자추출물군과 일반대조군에서는 각각 1점이었고, 붉게 염색된 지방미세과립이 주정대조군에서는 고도 내지 중등도로 세포질내에 침윤(infiltration)된 반면 오미자추출물군에서는 경도로 침윤되었다. 본 연구에서 오미자추출물은 경시적으로 혈중 알코올 분해율을 높여주고, 간 조직의 중성지방량과 간기능치(${\gamma}$-GT, ALT)를 감소시키는 동시에 알코올성 지방변성을 완화시킨 것으로 나타났다. 본 연구 결과는 향후 오미자 가공식품의 개발과 관련한 기초 및 홍보자료로 활용 가능성이 높을 것으로 기대되는 한편 인체적용시험을 통해 알코올성 간 기능 보효 효과와 관련된 보다 정밀한 후속 연구가 필요할 것으로 사료된다.

• 사상체질의학회지
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• 제21권3호
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• pp.216-222
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• 2009

1. Objectives: This case study described a process of the patient diagnosed as acute hepatitis A treated by medication based on the Sasang Constitutional Medicine and acupuctural theraphy and obtained improved effects about several symptoms. 2. Methods: We diagnosed the male patient with acute hepatitis A as Tae-Eumin Exterior-Cold-Disease by the Cold in the Esophagus, and administered Mahwangbalpyo-tang and Handayeolso-tang according to clinical progress. And we evaluated the results by liver function test(LFT; AST, ALT, $\gamma$-GTP ), total bilirubin level and the visual analog scale(VAS) of symptoms. 3. Results and Conclusion: Almost symptoms (except for jaundice) at the time of admission were disappeared, and also jaundice (estimated by color of scleras and color of urine) was alleviated. Also level of AST, ALT, $\gamma$-GTP and total bilirubin decreased in laboratory test. We obtained positive effects to the therapy but we considered necessities of further comparative studies with control group.