• Journal of Nutrition and Health
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• 제20권4호
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• pp.237-245
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• 1987

Young male rats were fed for 10 and 20 days on different low-fat experimental diets ; 4% lard + 4% soybean oil, 4% sardine oil + 4% lard, 4% sardine oil with vitamin E+ 4% lard, 4% sardine oil with vitamin E+ 4% soybean oil. Lipid peroxide levels in liver were significantly higher in the sardine oil groups but the addition of vitamin Ehad no effects on the lipid peroxide values. Serum total cholesterol showed no significant difference among the groups but HDL-cholesterol in serum was increased in the sardine oil groups. However, the contents of serum triglyceride and phospholipid were unaffected by the replacement with sardine oil.

• 한국산학기술학회논문지
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• 제16권5호
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• pp.3323-3332
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• 2015

중년 남성 근로자에 대한 알코올 섭취와 혈청지질, 간기능검사치 및 비만지표와의 관련성을 알아볼 목적으로 2013년 1월부터 12월까지의 기간 동안에 종합건강검진을 받았던 30~59세의 남성 근로자 2,175명을 대상으로 이들의 1일 알코올 섭취량과 혈압, 혈청지질, 간기능검사치 및 비만지표와의 관계를 검토하였다. 연구결과, 혈압은 음주량의 증가와 함께 유의하게 높은 값을 보였으며, LDL-콜레스테롤 및 중성지질(TG)도 음주량이 증가할수록 높아지는 경향을 보였으나 HDL-콜레스테롤은 음주량이 증가할수록 낮아지는 경향을 보였다. AST, ALT, ALP 및 ${\gamma}$-GTP도 음주량이 증가할수록 높아지는 경향을 보였으나 비만지표에서는 뚜렷한 차이가 없었다. 혈압(SBP, DBP), 혈청지질치(HDL-C, LDL-C, TG) 및 간기능검사치(AST, ALT)가 비정상으로 높아질 위험비는 소량 음주군에 비해 다량 음주군에서 유의하게 상승하였다. 위와 같은 결과는 다량의 음주는 혈압, 혈청지질 및 간기능검사치 증가와 유의한 관련성이 있었으나 비만지표와는 유의한 관련성이 인정되지 않음을 시사하고 있다.

• 한국응용과학기술학회지
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• 제3권2호
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• pp.49-64
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• 1986

This experiment is carried out to study effect of choline-deficient diet on serum and liver lipid contents of male rats. The experimental animals use 36 male Sprague-Dawley rats weighing about $100{\pm}3g$. They are classified into 7 groups and fed to experimental diets which are composed of 0.8% choline-supplement of deficient diets in addition to 14% corn oil, 14% corn margarine and 14% lipids mixed with 4% corn oil and 10% corn margarine, respectively. After feeding for 4 weeks, I measure lipid concentration of serum and liver, and the result are as follows. 1. The choline-deficient diet group decreases slightly the rates of weight gain and feed efficiency as compared with those of the choline-supplement diet group, but increases liver weight. 2. The choline-dificient diet group decreases the serum concentrations of total cholesterol (TC), free cholesterol, HDL-choelsterol, VLDL, LDL-cholesterol and phospholipid (PL), but increases the contents of triglyceride and the ratios of cholesterol/HDL-cholesterol and triglyceride/PL, and indicates no remarkable-difference in the ratio of TC/PL. 3. As compared with the choline-supplement diet group, the choline-deficient diet group contains the higher liver contents of total lipids, free cholesterol and triglyceride, and gives little difference in the liver contents of total cholesterol and phospholipid(PL), and presents the higher ratios of VLDL, LDL-cholesterol/HDL-cholesterol and TG/PL in the liver.4. In the choline-deficient diet group, the coutents of serum and liver lipid is not influenced by the kind of dietary fat. On the other hand, the choline-supplemented diet group indicates a significantly lower content of phospholipid in the corn margarine-added diet group than in the corn oil-added diet group. As aforementioned results, I think that the choline-deficient diet induces fatty liver in male rats without relation to kind of fatty acid, and cholines-upplement diet with saturated fatty acid makes the more decrease of liver phospholipid than that with polyunsaturated fatty acid.

• 한국식품영양과학회지
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• 제23권4호
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• pp.568-571
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• 1994

This study was conducted to investigate the effect of Platycodi radix Saponin(PRS) on the reduction of lipid status in rats fed on high fat diet for 6 weeks after which lipid contents were measured in serum , liver and feces. The results obtained from this study are as follows : That the levels of total lipid, total cholesterol and triglyceride in serum and liver were significantly lower in the PRS group as compared with the control group. The contents of total lipid and total cholesterol excreted in the feces were tended to be slightly increase in PRS group compared to the control group, which were not significant.

• Journal of Nutrition and Health
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• 제23권7호
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• pp.467-476
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• 1990

This study was performed to investigate the effects of different fat diets on the serum lipid composition and on the morphology of liver tissue of the spontaneously hypertensive rats(SHR), Rats were fed for 17 weeks one of four fat diets, control(corn oil : beef tallow=1:1), beef tallow, grapeseed oil and soybean oil. Blood was withdrawn by heart puncture from each SHR, and used to determine the serum levels of total cholesterol, HDL-cholesterol, trigly ceride and phospholipid. No significant difference in body weight was observed in all diet groups. the total serum cholesterol was significantly lower in SHR fed grapeseed oil than that of other groups. The cholesterol lowering effect of grapeseed oil seemed to results from its high contents of phytosterol and linoleic acid. Also, serum HDL-cholesterol, triglyceride and phospholipid contents were low in all the groups. photochemical microscopic observations of the liver tissue revealed the congestion of sinosoid which is regarded as the characteristics of SHR, but no significant difference was observed among the groups.

• Journal of Ginseng Research
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• 제12권1호
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• pp.76-86
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• 1988

쥐에게 물대신 12% 에탄올(대조군) 또는 인삼사포닌 분획을 포함한 12% 에탄올(시험군)을 6일간 투여한 후 간과 혈청에서의 acetaldehyde 농도와 간의 [$NAD^+$]/ [NADH] 및 [$NADP^+$]/[NADPH] 비율을 조사하였다. 대조군의 간과 혈청의 acetaldehyde 농도는 물로 사육한 정상군에 비해 훨씬 높았으나 물대신 인삼사포닌을 포함한 에탄올을 투여한 시험군의 경우는 정상군에 비해 약간 높았을 뿐이었으며 [$NAD^+$]/ [NADH] 비의 감소율도 대조군보다는 시험군이 훨씬 작았다. l-$^{l4}C$]-ethanol을 함유한 10% ethanol을 1ml 복강으로 투여하고 30분 후의 간의 지방질의 방사능을 분석한 결과 시험군의 간 지방질의 전체 방사능은 대조군보다 훨씬 낮았고 인산지방질, 콜레스테롤, 지방산, 중성지방과 같은 지방질의 분석결과는 에탄올 투여로 인한 인산지방질 생합성 저하와 지방산 및 중성지방의 생합성 증가현상이 인삼사포닌의 투여로 개선되는 것으로 관찰되었다.

• Journal of Nutrition and Health
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• 제16권1호
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• pp.10-20
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• 1983

In order to establish tissue lipid status in animal on feeding of various dietary fat and oils, each group of rats was fed a semisynthetic diet containing 10%(w/w) mackerel oil (MO), eel oil (EO), soybean oil (SO), rapeseed oil (RO) or beef tallow (BT) for 1, 2 and 4 weeks, After each feeding period, levels of cholesterol, triglyceride and phospholipid were measured in serum. Fatty acid ${\leftarrow}$ composition was also investigated in serum and tissue lipids. Levels of total serum cholesterol were lower but HDL-cholesterol were higher in fish oil groups, which resulted in significantly higher ratio of HDL to total cholesterol in the fish oil groups. Fish oil groups, in general, also had lower levels of serum triglyceride and phospholipid than other groups, but S0 group maintained as low phospholipid levrl as fish oil groups. Fatty acid composition of dietary fat was reflected in all the tissues investigated but with varying degrees. Very long chain fatty acids, specific components exclusively found in fish oils were most well reflected in liver and relatively well in serum, whereas linoleic acid and erucic acid of SO and RO in the diet were better shown up in heart and adipose tissue. It attracted a particular attention that major proportions of long chain monoenoic acids $(C_{22:1})$ occurring both in MO and RO were detected separately in liver and heart plus adipose tissue, the result of which strongly indicates that there is a significant difference in metabolism between isomers $(C_{22:1}\;w\;11\;and\;C_{22:1}\;w\;9,\;respectively)$. It is suggested from this study that differences in lipid status as well as in the levels of serum lipids result from uniqueness in metabolism of each different fatty acid and give rise to distinguishable change in serum lipoprotein pattern, followed by diet with different fat sources.

• Nutrition Research and Practice
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• 제8권2호
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• pp.172-176
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• 2014

BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolic syndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris (C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris water extract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), and proinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulin resistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatic total lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) levels were reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly by reducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.

• 동아시아식생활학회지
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• 제8권1호
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• pp.9-19
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• 1998

The purpose of this study was to investigate the effect of calcium intake on lipid contents and enzyme activities in rats of different ages. Lipid levels in serum and liver and GOT, CPK and LDH activities in serum were compared in rats of different ages(4 weeks and 10 months) that were fed various levels of calcium(50, 100, 200% of requirement)for 3 weeks. Body weight gain and feed efficiency ratio were significantly higher in young rats than in adults. Serum calcium level was increased by elevation of calcium intake levels were decreased. Liver phospholipid and triglyceride levels in the high-cal-terol and triglyceride levels were decreased. Liver phospholipid and triglyceride levels in the high calcium group were significantly lower than those in other groups. Serum GOT and LDH activities of adults were significantly higher in low-calcium group than those in adequate/high-calcium groups. However, serum CPK activity of adults was significantly higher in high-calcium group than that in low/adequate-calcium groups. The results of this study suggest that adequate calcium intake may have protective effects ont he alteration of lipid and enzyme activity in rats.

• Journal of Nutrition and Health
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• 제36권3호
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• pp.245-254
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• 2003

The aim of this study was to investigate the effects of P/S ratio of fatty acid and antioxidant (vitamin E, selenium) supplements on the serum lipid levels and hepatic antioxidant enzyme activity in rats. Female 16-week-old Sprague-Dawley rats were fed 6 different experimental diets for 4 weeks. While the peroxidizability index (PI) levels of fatty acids in the experimental diets were fixed at 81.22, the levels of P/S ratio of fatty acids were formulated at 0.38, 1.00, 4.81 (LP, MP, HP). These diets were supplemented with vitamin E (1,000 mg/kg diet) and selenium (2.5 mg/kg diet) (LP-S, MP-S, HP-S). This study showed that the serum concentrations of total-cholesterol and HDL-C increased with the increasing of the P/S ratio in the diet (p <0.05). Antioxidant supplementation significantly lowered the concentrations of triglyceride (TG) and VLDL-C of serum (p<0.05). Levels of thiobarbituric acid reactive substance (TBARS) in the liver tended to decrease with the increasing of the P/S ratio in the diet (p<0.001), but antioxidant enzyme activity in the liver was not significantly different. In addition, antioxidant supplementation significantly lowered TBARS level in the liver (p<0.05), but had no effect on antioxidant enzyme activity except for glutathione reductase (p<0.05). In conclusion, it is necessary to consider the properties of dietary fatty acids and antioxidants supplementation for the prevention of cardiovascular diseases.