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Effects of P/S Ratio of Fatty Acids and Antioxidants Supplement on Serum Lipids Levels and Hepatic Antioxidants Enzyme Activities in Rats  

강민정 (한양대학교 생활과학대학 식품영양학과)
이은경 (한양대학교 생활과학대학 식품영양학과)
이상선 (한양대학교 생활과학대학 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.36, no.3, 2003 , pp. 245-254 More about this Journal
Abstract
The aim of this study was to investigate the effects of P/S ratio of fatty acid and antioxidant (vitamin E, selenium) supplements on the serum lipid levels and hepatic antioxidant enzyme activity in rats. Female 16-week-old Sprague-Dawley rats were fed 6 different experimental diets for 4 weeks. While the peroxidizability index (PI) levels of fatty acids in the experimental diets were fixed at 81.22, the levels of P/S ratio of fatty acids were formulated at 0.38, 1.00, 4.81 (LP, MP, HP). These diets were supplemented with vitamin E (1,000 mg/kg diet) and selenium (2.5 mg/kg diet) (LP-S, MP-S, HP-S). This study showed that the serum concentrations of total-cholesterol and HDL-C increased with the increasing of the P/S ratio in the diet (p <0.05). Antioxidant supplementation significantly lowered the concentrations of triglyceride (TG) and VLDL-C of serum (p<0.05). Levels of thiobarbituric acid reactive substance (TBARS) in the liver tended to decrease with the increasing of the P/S ratio in the diet (p<0.001), but antioxidant enzyme activity in the liver was not significantly different. In addition, antioxidant supplementation significantly lowered TBARS level in the liver (p<0.05), but had no effect on antioxidant enzyme activity except for glutathione reductase (p<0.05). In conclusion, it is necessary to consider the properties of dietary fatty acids and antioxidants supplementation for the prevention of cardiovascular diseases.
Keywords
P/S ratio; peroxidizability index; serum lipid concentration; TBARS; antioxidant;
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1 Annual report on the cause of death statistics, National statistical office, Seoul, 1996
2 Lee JH, Fukumoto M, Nishida H, Ikeda I, Sugano M. The interrelated effects of n-6/n-3 and polyunsaturated/saturated ratios of dietary fats on the regulation of lipid metabolism in rats. J Nutr 119: 1839-1899, 1989
3 Kuratko C, Pence BC. Changes in colonic antioxidant status in rats during long-term feeding of different high fat diets. J Nutr 121: 1562-1569, 1991
4 Vatassery GT, Bauer T, Dysken M. High doses of vitamin E in the treatment of disorders of the central nervous system in the aged. Am J Clin Nutr 70 (5) : 793-801, 1999
5 Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499-502, 1972
6 Lee YC, Kim IM, Chung EJ, Um YS, Kim SY, Ahn HS, Kim ST. Fatty acid intake, serum fatty acid composition and serum se concentration of elementary school children in Korea. Korean J Nutrition 32(7): 802-811, 1999
7 Pietrangelo A, Grandi R, Tripodi A, Tomasi A, Ceccarelli D, Ventura E, Masini A. Lipid composition and fluidity of liver mitochondria, microsomes and plasma membrane of rats with chronic dietary iron overload. Biochem Phamacol 39: 123-128, 1990   DOI   ScienceOn
8 Cosgrove JP, Church DF, Pryor WA. The kinetics of the autoxidation of polyunsaturated fatty acids. Lipids 22: 299-304, 1987   DOI
9 Kashiwagi A, Asahina T, Nishio Y, Ikebuchi M, Tanaka Y, Kikkawa R, Shigeta Y. Glycation, oxidative stress and scavenger activity: glucose metabolism and radical scavenger dysfunction in endothelial cells. Diabetes 45 (suppl 3) : S84-S86, 1996
10 Hwang HJ, Um YS, Chung EJ, Kim SY, Lee YC, Effect of dietary fatty acid and vitamin E supplementation on antioxidant system of the second generation rat brain sections. Korean J Nutrition 34(1): 14-22,2001
11 Pironi L, Ruggeri E, Zolezzi C, Savarino L, Incasa E, Belluzzi A, Munarini A, Piazzi S, Tolomelli M, Pizzoferrato A, Miglioli M. Lipid peroxidation and antioxidant status in adults receiving lipid-based home parenteral nutrition. Am J Clin Nutr 68: 888-893, 1998
12 Keen CL, Tamura T, Lonnerdal B, Hurley LS, Halsted CH. Changes in hepatic superoxide dismutase activities in alchoholic monkeys. Am J Clin Nutr 41: 929-932, 1985
13 Carlberg I, Mannervik B. Glutathione reductase. Meth Enzymology 113: 484, 1985   DOI
14 Hossain MS, Hashimoto M, Gamoh SM. Influence of docosahexaenoic acid on celebral lipid peroxide level in aged rats with and without hypercholesterolemia. Neurosci Letters 244: 157-160, 1998   DOI   ScienceOn
15 Burk RF. Recent developments in trace element metabolism and function: Newer roles of selenium in nutrition.J Nutr 119: 1051-1054, 1989
16 Claiborne A. Catalase activity, CRC handbook of methods for oxygen radical research, pp.283, 1984
17 Skuadorrit GV, Shi-Hua D, Brodie AE, Reed DJ, Wander RC. Effects of dietary oils an methyl ethyl ketone peroxide on in vivo lipid peroxidation and antioxidants in rat heart and liver. Lipid 29: 351-357, 1994   DOI
18 Park HS, Song JH. Effect of dietary fatty acids and the degree of fat unsaturation on fatty acid profile and lipid peroxidation status in rats. Korean Biochem J 25 (7) : 609-617, 1992
19 Chow CK, Reddy K, Tappel AL. Effect of dietary vitamin E on the activities of the glutathione peroxidase system in rat tissues. J Nutr 103: 618-624, 1973
20 Warhorm M, Guthenberg C, Bahr CV, Mannervik B. Glutathione transferases from human liver. Meth Enzymology 113: 499, 1985   DOI
21 Flohe L, Gunzler WA. Assays of glutathione peroxidase. Meth Enzymology 105: 114-121, 1984   DOI
22 Nam JH, Park HS, Effect of quality and quantity of dietary fats on the status of tocopherol and lipid peroxidation of plasma and tissue in rats. Korean J Nutrition 26(5) : 566-577, 1993
23 Kim SH, Hong MY. The changes of body fat accumulation, serum lipid and platelet functions in rat fed the diet containing different common oils in korea: sesame oil, perilla oil, rice bran oil and mixed oil. Korean J Nutrition 25 (5) : 513-523, 1993
24 Crow CK, Tappel AL. Response of glutathione peroxidase to dietary selenium in rats. J Nutr 104: 444-451,1974
25 Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474, 1974   DOI   ScienceOn
26 Sanders TA, Oakley FR, Miller GJ, Mitropoulos KA, Crook D, Oliver MF. Influence of n-6 versus n-3 polyunsaturated fatty acids in diets low in saturated fatty acids on plasma lipoproteins and hemostatic factors. Arterioscler Thromb Vasc Biol 17: 3449-3460, 1997
27 Nordoy A, Hatcher LF, Ullmann DL, Connor WE. Individual effects of dietary saturated fatty acids and fish oil on plasma lipids and lipoproteins in normal men. Am J Clin Nutr 57: 634-639, 1993
28 Diplock AT. Trace elements in human health with special reference to selenium. Am J Clin Nutr 45: 1313-1322, 1987
29 Reeves PG. Components of the AIN-93 diets as improvements in the AIN-76 diets. J Nutr 127: 838S-841S, 1997
30 Wilson PW, Zech LA, Gregg RE, Schaefer EJ, Hoeg JM, Sprecher DL, Brewer HB Jr. Estimation of VLDL cholesterol in hyper-lipidemia. Clin Chem Acta 151 (3): 285-291, 1985   DOI   ScienceOn
31 Hu ML, Frankel EN, Leibovitz BE, Tappel AL. Effect of dietary lipids and vitamin E on in vivo lipid peroxidation in rat liver and kidney homogenates. J Nutr 119: 1574-1582, 1989
32 Lee BY, Chang YK. Relationships between fatty acid intakes and serum lipid in postmenopausal Women. Korean J Nutrition 32(4):437-447,1999
33 Bradford M. A rapid and sensitive method for guanititation of microgram guantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976   DOI   ScienceOn
34 Horvath PM, Ip C. Synergistic effect of vitamin E and selenium in the chemoprevention of mammary carcinogenesis in rats. Cancer Res 43: 5335-5341,1983
35 Dougherty RM, Fong AKH, Iacono JM. Nutrient content of the diet when the fat is reduced. Am J Clin Nutr 48: 970-979, 1988
36 Kayden HJ, Wisniewski T. On the biological activity of vitamin E. Am J Clin Nutr 72: 201-202, 2000
37 Conner WE. Importance of n-3 fatty acids in health and disease. Am J Clin Nutr 71 (suppl): 171S-175S, 2000
38 Horwitt MK. Vitamin E: a reexamination. Am J Clin Nutr 29: 569, 1976
39 Phillipson BE, Rothrock DW, Connor WE, Harris WS, Illing-worth DR. Reduction of plasma lipids, lipoproteins, and apoproteins by dietary fish oils in patients with hypertriglyceridemia. N Engl J Med 312: 1210-1216, 1985   DOI   ScienceOn
40 Knezevic V, Mujovic VM, Milosevic A. Effect of vitamin E on erythrocyte enzyme and total antioxidant status in diabetic patients with ischemic heart disease. Srp Arh Celok Lek 128(7-8): 241-246,2000
41 Marklund S. Pyrogallol Autoixdation, CRC handbook of methods for oxygen radical research, pp.243, 1984
42 Bellisola G, Galassini S, Moshini G, Ploi G, Perona G, Guidi G. Selenium and glutathione peroxidase variations induced by polyunsaturated fatty acids oral supplementation in humans. Clin Chem Acta 205(1-2) : 75-85, 1992   DOI   ScienceOn
43 Meydani M, Macauley JB, Blumberg JB. Effect of dietary vitamin E and selenium on susceptibility of brain regions to lipid peroxidation. Lipids 23(5) : 405-409, 1988   DOI
44 Lands WEM, Hamazaki T, Yamazaki K, Okuyama H, Sakai K, Goto Y, Hubbard YS. Changing dietary patterns. Am J Clin Nutr 51: 991-993,1990
45 Sinnbuber RO, Yu TC. Characterization of the red pigment formed in 2-thiobarbituric acid determination of oxidation rancibility. Food Res 23: 620-629, 1958
46 L'abbe MR, Trick KD, Beare-Rogers JL. Dietary n-3 fatty acids affect rat heart, liver and aorta protective enzyme activities and lipid peroxidation. J Nutr 121: 1331-1340, 1991
47 Witting LA, Horwitt MK. Effect of degree of fatty acid unsaturation in tocopherol-deficiency induced creatinuria. J Nutr 82:19-24,1964
48 Meydani M, Felicia N, Barry G, Nancy F, Margo W, Sherwoord LG. Effect of long term fish oil supplementation on vitamin E status and lipid peroxidation in women.J Nutr 121: 484-491, 1991
49 Choi CH, Yoon HS. Docosahexaenoic acid (DHA) and aging. Korean J Nutrition 27(6): 646-654, 1994
50 Park SM, Ahn SH, Choi MY, Choi SB. The effect of vitamin E supplementation on insulin resistance and oxidative stress in Sprague dawley rats fed high ω-6 polyunsaturated fat diet. Korean J Nutrition 32(6): 644-653, 1999
51 Aebi HE. Catalase, Methods in enzymatic analysis 3rd, Vol. 3, pp.273-285, 1984
52 Pigeolet E, Corbisier P, Houbion A. Glutathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides, and oxygen derived free radicals. Mech Ageing Dev 51: 283-297, 1990   DOI   ScienceOn