• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.51.2-51.2
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• 2015

Understanding interfacial phenomena has been one of the main research issues not only in semiconductors but only in life sciences. I have been trying to meet the atomic scale surface and interface analysis challenges from semiconductor industries and furthermore to extend the application scope to biomedical areas. Optical imaing has been most widely and successfully used for biomedical imaging but complementary ion beam imaging techniques based on mass spectrometry and ion scattering can provide more detailed molecular specific and nanoscale information In this presentation, I will review the 27 years history of medium energy ion scattering (MEIS) development at KRISS and DGIST for nanoanalysis. A electrostatic MEIS system constructed at KRISS after the FOM, Netherland design had been successfully applied for the gate oxide analysis and quantitative surface analysis. Recenlty, we developed time-of-flight (TOF) MEIS system, for the first time in the world. With TOF-MEIS, we reported quantitative compositional profiling with single atomic layer resolution for 0.5~3 nm CdSe/ZnS conjugated QDs and ultra shallow junctions and FINFET's of As implanted Si. With this new TOF-MEIS nano analysis technique, details of nano-structured materials could be measured quantitatively. Progresses in TOF-MEIS analysis in various nano & bio technology will be discussed. For last 10 years, I have been trying to develop multimodal nanobio imaging techniques for cardiovascular and brain tissues. Firstly, in atherosclerotic plaque imaging, using, coherent anti-stokes raman scattering (CARS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) multimodal analysis showed that increased cholesterol palmitate may contribute to the formation of a necrotic core by increasing cell death. Secondly, surface plasmon resonance imaging ellipsometry (SPRIE) was developed for cell biointerface imaging of cell adhesion, migration, and infiltration dynamics for HUVEC, CASMC, and T cells. Thirdly, we developed an ambient mass spectrometric imaging system for live cells and tissues. Preliminary results on mouse brain hippocampus and hypotahlamus will be presented. In conclusions, multimodal optical and mass spectrometric imaging privides overall structural and morphological information with complementary molecular specific information, which can be a useful methodology for biomedical studies. Future challenges in optical and mass spectrometric imaging for new biomedical applications will be discussed.

• Journal of Preventive Medicine and Public Health
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• 제32권1호
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• pp.9-16
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• 1999

Objectives. Few studies have attempted to explain the association between socioeconomic status (SES) and obesity in Korea Especially the studies on same race with different SES at same time are good for SES influence estimation. The present study highlights the influence of SES of parents on adolescent obesity. The subjects are the same race, but live in different areas, with different SES, Seoul and Yangpung in Korea, and Yanbian in China. Methods. The cross-sectional study was conducted in Mar-Jun 1996. We carried out anthropometry, 24 hour-recall diet survey, self-reported questionnaire about sociodemographic characteristics and physical activity. For SES indicators, we used income and education of parents. Results. The relationship between SES of parents, defined as educational status, and obesity in boys in Korea and China is direct, but not significant. The relationship is inverse and nonsignificant among Korean girls, However, for the Korean girls in severe obesity, the relationship is significant. For the Korean-Chinese girls, there is an inverse relationship between the education level and obesity but a direct relationship between the income level of parents and obesity. For the Korean adolescent, there are a direct association between the SES of parents and the nutrients factors such as energy, protein, and fat intake. The energy expenditure of adolescent has no relationship with SES of parents. Conclusion. Among boys, the higher the SES, the greater the risk of obese. Among girls in Korea and Korean-Chinese, on the other hand, the lower the educational status of parents, the greater the risk of obese.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2011년도 한국컴퓨터종합학술대회논문집 Vol.38 No.1(B)
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• pp.25-28
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• 2011

최근 사이언스 컴퓨팅 분야에서 대용량 데이터가 발생하고 있고 컴퓨팅 자원들의 수요도 급증하고 있다. 이로 인해 클라우드 컴퓨팅 기술의 필요성이 중요시되고 있고 전 세계적으로 급속히 발전하고 있다. 천문 연구 분야에서 관측기기의 발전으로 대용량의 천문 데이터가 생산되고 있다. 이를 처리하기 위하여 다양한 클라우드 컴퓨팅 기술을 이용한 데이터 분석 환경이 요구되고 있다. 이러한 환경을 구축하기 위해서는 가상 인프라 자원을 효율적으로 관리할 수 있어야 한다. 현재 가상 인프라 자원을 관리하기 위해 오픈 소스를 개발하는 프로젝트들이 활발히 진행되고 있다. 본 논문에서는 오픈 소스인 Eucalyptus와 OpenNebula의 기능적인 측면을 크게 10가지로 분류하여 장단점을 비교 분석하였고 OpenNebula의 세 가지 특징인 중앙 집중화된 구조, live migration과 suspend/resume 기능, 커스터마이징 기능이 대용량 천문 응용 수행을 위한 환경을 구축하는데 반드시 필요하기 때문에 OpenNebula를 채택하였다. OpenNebula를 사용해 구축한 사이언스 클라우드 테스트베드는 NFS 서버의 별도 구성을 통한 성능 개선, 하이브리드 클라우드 컴퓨팅 환경 구축, B클래스 사설 네트워크 구성, GUI 기반 사이언스 클라우드 테스트베드 관리 및 모니터링 기능의 4가지 특징을 가지고 있다. 앞으로도 천문 연구자들에게 안정적인 서비스를 제공하기 위하여 관련 연구를 계속할 것이다.

• Ocean and Polar Research
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• 제24권1호
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• pp.115-121
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• 2002

Yubu Island is located within the estuary of the Geum River, South Chungcheong Province $(35^{\circ}59'N,\;126^{\circ}36'E)$, Korea. The island is surrounded by a broad and sandy mudflat, which is typical in the west coast of Korea, and is located 4km off from Gunsan City. Less than 100 humans live on the island, occupying 30 houses. After we discovered that this island was a very important waterbird habitat especially for the East Asian subspecies of the Eurasian oystercatcher, Haematopus ostralegus osculans. Waterbirds were monitored once every month from August 1999 to July 2000. The highest number of oystercatchers counted was 3,200 in December 1999, and the birds seemed to remain in the area continuously from September to next February. About 200 breeding and non-breeding birds remained during the breeding season. In August, early migrants returned to the island, with the number reaching 1,060 individuals. This island is also very important for other waterbirds. Endangered or significant species occurring at this site, and their maximum counts were: chinese egret Egretta eulophotes (5), black-faced spoonbill Platalea minor (17), brant Branta bernicla (1), common shelduck Tadorna tadorna (8,000), hooded crane Grus monacha (2), spoon-billed sandpiper Ewynorhynchus pygmeus (7), dunlin Calidris alpina (6,500), great knot Calidris tenuirostris (24,000), far eastern curlew Numenius madagascariensis (2,500), spotted greenshank Tringa guttifer (4) and Saunders's gull Larus saundersi (1,200). During the 12 month survey period, we observed 52 waterbird species and the total of the maximum counts for the separate species was 73,308, implying that perhaps 150,000 birds could be using the immediate area, if a turnover rate of 2 was assumed.

• 한국주거학회논문집
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• 제16권5호
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• pp.57-66
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• 2005

Korea has experienced serious changes during the last several decades of industrialization. Limited land resources and excessive rural-to-urban migration inevitably resulted high-rise apartment housing development. However, apartment housing couldn't follow up social change and residents' needs. Turning into the 21st century, there are great demands for the diversification of housing style and amenity of housing, which include enhancing community lift through proper collective environment. To solve these problems, cohousing has been introduced in Scandinavian countries. A primary goal of cohousing is the desire of residents to live in a socially supportive setting. People can do housework together and also can promote active mutual relationship among residents in the community. Physical feature of cohousing, in combination with social organization factors, may serve to enhance or support the sense of community sought by residents. In this point of view, the purpose of this study is to find out the physical feature of cohousing in Denmark and Sweden. First, it is to figure out the background and development of cohousing in Denmark and Sweden. Then, by making clear physical features between similarity and difference of two countries of cohousing through case study; such as housing type, the circulation patterns, common facilities and etc. This paper could suggest a possibility of application of cohousing in Korea to present how they encourage emphasize design aspects that increase the possibilities for social contacts and the sense of community. Also, it goes on to suggest that the educational program and the support from the government.

• Parasites, Hosts and Diseases
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• 제59권3호
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• pp.235-249
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• 2021

Leptin is a type of adipokine mainly produced by adipocytes and reported to be overproduced in prostate cancer. However, it is not known whether it stimulates the proliferation of prostate cells. In this study, we investigated whether benign prostatic hyperplasia epithelial cells (BPH-1 cells) infected with Trichomonas vaginalis induced the proliferation of prostate cells via a leptin signaling pathway. To investigate the effect of crosstalk between adipocyte leptin and inflamed epithelial cell in proliferation of prostate cells, adipocytes 3T3-L1 cells were incubated in conditioned medium of BPH-1 cells infected with T. vaginalis (T. vaginalis-conditioned medium, TCM), and then the adipocyte-conditioned medium (ATCM) was identified to cause proliferation of prostate cells. BPH-1 cells incubated with live T. vaginalis released pro-inflammatory cytokines, and conditioned medium of these cells caused migration of adipocytes. When prostate stromal cells and BPH-1 cells were incubated with adipocyte conditioned medium containing leptin, their growth rates increased as did expression of the leptin receptor (known as OBR) and signaling molecules such as JAK2/STAT3, Notch and survivin. Moreover, blocking the OBR reduced this proliferation and the expression of leptin signaling molecules in response to ATCM. In conclusion, our findings show that inflamed BPH-1 cells infected with T. vaginalis induce the proliferation of prostate cells through leptin-OBR signaling. Therefore, it is likely that T. vaginalis contributes to prostate enlargement in BPH via adipocyte leptin released as a result of inflammation of the prostate.

• Parasites, Hosts and Diseases
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• 제59권6호
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• pp.547-556
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• 2021

Our objective was to investigate whether inflammatory microenvironment induced by Trichomonas vaginalis infection can stimulate proliferation of prostate cancer (PCa) cells in vitro and in vivo mouse experiments. The production of CXCL1 and CCL2 increased when cells of the mouse PCa cells (TRAMP-C2 cell line) were infected with live T. vaginalis. T. vaginalis-conditioned medium (TCM) prepared from co-culture of PCa cells and T. vaginalis increased PCa cells migration, proliferation and invasion. The cytokine receptors (CXCR2, CCR2, gp130) were expressed higher on the PCa cells treated with TCM. Pretreatment of PCa cells with antibodies to these cytokine receptors significantly reduced the proliferation, mobility and invasiveness of PCa cells, indicating that TCM has its effect through cytokine-cytokine receptor signaling. In C57BL/6 mice, the prostates injected with T. vaginalis mixed PCa cells were larger than those injected with PCa cells alone after 4 weeks. Expression of epithelial-mesenchymal transition markers and cyclin D1 in the prostate tissue injected with T. vaginalis mixed PCa cells increased than those of PCa cells alone. Collectively, it was suggested that inflammatory reactions by T. vaginalis-stimulated PCa cells increase the proliferation and invasion of PCa cells through cytokine-cytokine receptor signaling pathways.

• BMB Reports
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• 제55권6호
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• pp.267-274
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• 2022

Molecular imaging is used to improve the disease diagnosis, prognosis, monitoring of treatment in living subjects. Numerous molecular targets have been developed for various cellular and molecular processes in genetic, metabolic, proteomic, and cellular biologic level. Molecular imaging modalities such as Optical Imaging, Magnetic Resonance Imaging (MRI), Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), and Computed Tomography (CT) can be used to visualize anatomic, genetic, biochemical, and physiologic changes in vivo. For in vivo cell imaging, certain cells such as cancer cells, immune cells, stem cells could be labeled by direct and indirect labeling methods to monitor cell migration, cell activity, and cell effects in cell-based therapy. In case of cancer, it could be used to investigate biological processes such as cancer metastasis and to analyze the drug treatment process. In addition, transplanted stem cells and immune cells in cell-based therapy could be visualized and tracked to confirm the fate, activity, and function of cells. In conventional molecular imaging, cells can be monitored in vivo in bulk non-invasively with optical imaging, MRI, PET, and SPECT imaging. However, single cell imaging in vivo has been a great challenge due to an extremely high sensitive detection of single cell. Recently, there has been great attention for in vivo single cell imaging due to the development of single cell study. In vivo single imaging could analyze the survival or death, movement direction, and characteristics of a single cell in live subjects. In this article, we reviewed basic principle of in vivo molecular imaging and introduced recent studies for in vivo single cell imaging based on the concept of in vivo molecular imaging.

• Journal of Ginseng Research
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• 제48권2호
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• pp.171-180
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• 2024

Background: Epimers of ginsenoside Rg3 (Rg3) have a low bioavailability and are prone to deglycosylation, which produces epimers of ginsenoside Rh2 (S-Rh2 and R-Rh2) and protopanaxadiol (S-PPD and R-PPD). The aim of this study was to compare the efficacy and potency of these molecules as anti-cancer agents. Methods: Crystal violet staining was used to study the anti-proliferatory action of the molecules on a human epithelial breast cancer cell line, MDA-MB-231, and human umbilical vein endothelial cells (HUVEC) and compare their potency. Cell death and cell cycle were studied using flow cytometry and mode of cell death was studied using live cell imaging. Anti-angiogenic effects of the drug were studied using loop formation assay. Molecular docking showed the interaction of these molecules with vascular endothelial growth factor receptor-2 (VEGFR2) and aquaporin (AQP) water channels. VEGF bioassay was used to study the interaction of Rh2 with VEGFR2, in vitro. Results: HUVEC was the more sensitive cell line to the anti-proliferative effects of S-Rh2, S-PPD and R-PPD. The molecules induced necroptosis/necrosis in MDA-MB-231 and apoptosis in HUVEC. S-Rh2 was the most potent inhibitor of loop formation. In silico molecular docking predicted a good binding score between Rh2 or PPD and the ATP-binding pocket of VEGFR2. VEGF bioassay showed that Rh2 was an allosteric modulator of VEGFR2. In addition, SRh2 and PPD had good binding scores with AQP1 and AQP5, both of which play roles in cell migration and proliferation. Conclusion: The combination of these molecules might be responsible for the anti-cancer effects observed by Rg3.

• 한국어류학회지
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• 제22권4호
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• pp.238-248
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• 2010

강원도 고성군 북천의 어류 분포 및 군집 특성을 알아보기 위하여 2009년 3월부터 9월까지 조사를 실시하였다. 본 조사에서 확인된 어종은 모두 12과 31종이었으며, 개체수에서 우점종은 Z. koreanus (30.0%), 아우점종은 P. kaibarae (9.4%), R. steindachneri (9.3%)로 나타났으며, 생체량에서 우점종은 T. hakonensis (35.5%), 아우점종은 Z. koreanus (22.5%), C. pacifica (7.5%)였다. 출현종 중 환경부지정 멸종 위기종 II급인 P. kaibarae와 P. sinensis가, 한국 고유종은 8종이 출현하였다. 회유성 어종 중 소하성 어종은 T. hakonensis, O. masou masou, O. keta, G. aculeatus가 서식하고 있었으며, 양측회유성 어류인 P. altivelis altivelis, G. urotaenia가 출현하였다. 고성 북천에 분포하지 않았던 서해와 남해로 흐르는 하천에 분포하던 종 중에 본 조사에서 서식이 확인된 Z. koreanus, Z. platypus, S. microdorsalis, L. andersoni, C. herzi는 1980년대 이입되었고, P. herzi, P. parva, S. multimaculatus, M. mizolepis, K. rotundicaudata, S. asotus, O. interrupta 등은 1990년대 이후에 이입된 것으로 분석되었다.