This study was to investigate the effects of a silk peptide, and prune and raspberry mixture on the body fat, serum lipid composition and fat distribution in Korean women during the time they received these supplements. During the 4 month period of this research (June to October, 2002), 75 women (average age 22.9 yr) were selected as subjects. Their nutrient intake was investigated by use of a questionnaire and the 24-hour recall method. Antropometric assessments of the subjects were investigated by using the SBIA Method (Segmental Bioimpedance Assay, Inbody 3.0). The results were as follows: their mean body weight was 59.3 kg, their mean body height was 161.7 cm and their mean Basal Metabolic Indices (BMI) was 22.7. The status of their dietary fiber intake significantly increased (p < 0.05) after use of the supplements. Their body fat significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05), and their percent of body fat signiflcantly decreased after supplementation (p < 0.05). Their triceps and suprailiac circumference significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05). Their waist circumferences and waist-hip ratios (WHR) significantly decreased after both the $2^{nd}$and $3^{rd}$ stage of supplementation (p < 0.05). Their total cholesterol significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their LDL-cholesterol and LDL / HDL ratios significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their 4-HNE and total antioxidant statuses significantly decreased following the $2^{nd}$ and $3^{rd}$stage of supplementation, as compared to their baseline results and those obtained following the $1^{st}$ stage of supplementation (p <0.05). Their $2^{nd}$ stage supplementation results were significantly negatively correlated with their triceps measurements and WHR (p < 0.05). Their $3^{rd}$stage supplementation results were significantly negatively correlated with their anterior thigh measurements (p < 0.05). The above results show that prune, raspberry, silk peptide and dietary fiber supplementation to a routine diet improves body fat distribution, total cholesterol, and triglyceride levels. The decrease of abdominal fat and WHR were especially noteworthy. This implies a decrease of risk factors. (Korean J Community Nutrition 8(3) : 356~367, 2003)003)
Histochemical distribution and varietal variation of schizandrin and oil compounds, and the changes of their extraction yield were investigated in fruits of collected Schizandra chinensis including Inje cultivar. In histochemical analysis on the distribution of schizandrin and oil in fruits of Inje cultivar, higher concentrations of them were found in the seed (1.01% and 27.6%, respectively) than in the epicarp and mesocarp of the whole fruit. Average contents of schizandrin in fruits and oil in seeds of collected lines were 0.84% and 27.9%, respectively. The mean composition of fatty acids in seeds oil was 3.6% of palmitic acid, 0.6% of stearic acid, 19.7% of oleic acid, 73.0% of linoleic acid, and 3.1% of linolenic acid, showing high composition(95.8%) of total unsaturated fatty acid. Oil extracted from seeds of Inje cultivar contained 4.29% of schizandrin, indicating that seed oil contained much schizandrin, a bioactive lipid-soluble compound. Compared with 80% methanol extraction in fruits and seeds, yields of schizandrin and oil were lower showing 23.8% and 17.3%, respectively in boiling water extraction of the fruits and seeds without grinding. The seeds soaked with water during four months contained 1.18% of schizandrin and 25.2% of oil, whose contents were similar to those of the seeds stored at room temperature. These results demonstrated that the seed in the whole fruit could be utilized as a source to extract its functional oil and bioactive lipid-soluble compounds like schizandrin, especially after using Schizandra fruits for the beverage manufacture.
Background and Objectives : Atopic dermatitis is a recurrent or chronic eczematous skin disease with severe pruritus,and has increased in Korea. Although the pathogenic mechanisms of atopic dermatitis are yet unknown, recently skin barrier dysfunction and hyperresponsive Th2 cells in the acute phase have been reported as important mechanisms. Cheonggi-san(CGS) is used in oriental clinics for treatingacute skin lesions of eczema or urticaria. There have been no studies on the therapeutic mechanism of CGS for curing atopic dermatitis. We aimed to find out the therapeutic effects of its internaluse on atopic dermatitis-like skin lesions, induced in NC/Nga mice by the mite antigen D. pteronyssinus and disrupting skin barrier. Materials and Methods : The NC/Nga mice were classified into three groups: control group, atopic dermatitis elicitated group(AD), and CGS treated group (CT). Atopic dermatitis-like skin lesions were induced on the back of female NC/Nga mice, 12 weeks of age, by tape stripping, 5% SDS applied to disrupt skin barrier and painting 3 times a week with D. pteronyssinus crude extract solution for 3 weeks. CT was treated with CGS orally after atopic dermatitis was elicitated. We observed changes of skin damage, mast cells, substance P, angiogenesis, skin barrier, Th2 cell differentiation, nuclear factor-${\kappa}B(NF-{\kappa}B)$ p65 activation and COX-2 in NC/Nga mice with atopic dermatitis-like skin lesions. Results : The skin damages as eczema were seenin AD, but mitigated in CT. The degranulated mast cells in dermal papillae increased in AD, but decreased in CT. The substance P positive reacted cells in CT remarkably decreased. The angiogenesis increased in AD, but decreased in CT. The decrease of lipid deposition and ceramide in AD was seen, but anincrease of lipid deposition and ceramide in CT was seen. The distribution of IL-4 positive reacted cells in dermal papillae increased in AD, but decreased in CT. The distribution of NF-${\kappa}B$ p65 positive reacted cells & COX-2 positive reacted cells in CT decreased. Conclusion : The results may suggest that the CGS per os decreases the dysfunction of the skin barrier, inhibits Th2 cell differentiation and inhibits NF-${\kappa}B$ p65 activation in NC/Nga mice with atopic dermatitis-like skin lesions.
The purpose of this study was to investigate the effects of the nutrition therapy and drug(simvastatin) treatment on the levels of blood lipids for the hyperlipidemic patients according to genetic polymorphism of apo CIII. Subjects of the study consisted of 43(male: 9, female: 34 )hyperlipidemic patients registered to Kyung Hee Medical Center, Intakes of nutrients for the subjects were determined by 24-hr recall method through a personal interviews. The subjects were instructed to take the hypolipidemic and hypocholesterolemic diets. Compliance was monitored through food records, personal interview, and body-weight measurements. The patients changed their basal diet containing 40. 1g fat(182.7mg cholesterol./day) to a diet containing 30.7g fat(139.2mg cholesterol/day) after 12 weeks of nutrition therapy. The distribution of genotypes for apo CIII by Msp I was 32.5% of TT, 50.0% of TC, and 17.5% of CC. aT the beginning of the study, the level of blood triglyceride was the highest for patients with TT and TC. Following the nutrition therapy, patients with CC exhibited the largest drop of blood triglyceride. Following simvastatin treatment with nutrition therapy, blood LDL-cholesterol decreased in most of the patiens with TT. Meanwhile the distribution of genotypes for apo CII by the Fok I was 10.0% of TT, 47.5% of TC, and 42.5% of CC. The level of blood triglyceride was the highest for patients with CC. Following the nutrition therapy, patients with TC exhibited a significant drop for the blood triglyceride. Following simvastatin treatment with nutrition therapy the level of blood LDL-cholesterol decreased in most of the patients with CC. We concluded that dietary habits and food have been changed by nutrition therapy. And blood lipid levels have been decreased by the restriction of intakes of energy, fat and cholesterol. There was variation in the levels of blood lipids according to apo CII polymorphisms. The level of blood lipids responded to nutrition therapy and drug treatment in different was according to genetic polymorphisms. Accordingly, the choice of individualized therapy based on the patient\\`s genetic polymorphism is very important for effective therapy(Korean J Nutrition 33(8) : 813-823, 2000)
Objectives: This study was designed to determine the levels of polybrominated diphenyl ethers (PBDEs) in breast milk and to evaluate the relations with factors affecting these levels. Methods: The congener levels of PBDE in 22 samples of breast milk were analyzed using a high resolution gas chromatograph with a high resolution mass detector. In accordance with our standard operating procedures, the recoveries of internal standards had to range between 68% and 118%. Since the distribution of PBDE concentrations is close to log-normal, the data were logarithmically transformed before analysis. Test subjects were healthy primipara and multipara mothers with a mean age of 32 (SD = 2.7) in 2006. Results: Seven PBDE congeners (BDE-28, 47, 99, 100, 153, 154, and 183) were detected and identified in all of the pooled breast milk samples, indicating widespread contamination from PBDEs in the environment in Korea. Residue levels of total PBDEs (sum PBDEs from tri- to hepta-BDE) ranged from 0.84-13.1 ng/g lipid with median and geometric mean levels of 2.6 ng/g lipid and 2.74 ng/g lipid, respectively. PBDE congeners 47, 99 and 153 markedly predominated and accounted for about 75% of the amount of the PBDE congeners analyzed. BDE-47 was the dominant congener in most samples, whereas BDE-153 was predominant in a few (n = 7/22). BDE-47 was highly correlated with total PBDEs (r = 0.987, p < 0.01). In analyses of the differences of the means of log transformed breast milk PBDE levels for groups of potential covariates, only breast milk BDE-47 and BDE-99 levels were significantly associated with fish (p < 0.05) and meat consumption (p < 0.01). However, we did not find significant correlations between PBDE levels and maternal age, body mass index (BMI), parity, job presence and smoking status. Conclusions: Our findings are mainly limited due to the small sampling size and low doses of PBDEs exposure. Background and human exposure data of PBDEs is lacking, and longitudinal investigations into the environment and biota are encouraged to determine the health impact on future populations in Korea.
The Journal of the Korean life insurance medical association
/
v.2
no.1
/
pp.143-151
/
1985
In medical selection of Daehan Education Insurance, We doubted that 140 cases(male 117 cases, female 23 cases) might have the fatty infiltration of the liver by ultrasonography. We examined the weight and the hypertension of 140 patients by biochemically analyzing the blood extracted under fasting according to the age-distribution. The results are as follows: 1. 90% out of them had body weight than normal subject. 34.29% out of them were hypertension patients and most of them were $30{\sim}39's$ and $40{\sim}49's$. 2. As compared with the normal subjects in serum lipid value, HDL cholesterol value was low, but total cholesterol, Triglyceride, phospholipid and total lipid value were significantly high. Especially the statistical value of Triglyceride was very significantly high. 3. Total abnormal rate of sGOT value in Liver function test is 34.43% and the mean value of it is 66 unit and the abnormal rate of sGPT value is all 3.14% and the value of it is 70 unit. 4. The abnormal rate of Alkaline phosphatase value is 9.29% and the abnormal rate of total Bilirubine value is 14.29%. As HBsAg positive rate is 2.14% and HBs Ab positive rate is 31.43%, the positive rate of HBsAg represents Lower positive rate than Korean mean value. 5. The abnormal rate of the fasting blood glucose is 15.00% and the abnormal rate of Blood sugar pc 2hrs is 30.71%. 6. The abnormal case of protein, Albumin, Globulin, BUN, craetinine and Hemoglobin value was not found to any of them and in the above liver function test except serum lipid value, the rate not showing the abnormal value represents 45.3%(male) and 52.17%(female).
Several samples of tile dried purple laver grown and processed at the major laver producing districts, i. e Mokpo, Wando, Hadong and Jangrim, along the southern coast of Korea were Quantitatively investigated to determine composing patterns of the fatty acids by gas-liquid chromatography. The total lipid contents in dried purple laver of Hadong were $1.8\%$ being the highest value. Upon analyzing fatty acid composition, some differences were observed in their quantitative distribution at different growing places. Dried purple laver of Wando contained mainly $C_{16:0},\;C_{18:1},\;C_{16:1}\;and\;C_{17:0}$ fatty acids, that of Mokpo contained $C_{16:0},\;C_{20:5},\;C_{18:1}\;and\;C_{16:1}$ fatty acids, and that of Hadong $C_{16:0},\;C_{20:5},\;C_{18:0}\;and\;C_{20:1}$ fatty acids, and that of Jangrim $C_{20:5},\;C_{16:0},\;C_{18:1}\;and\;C_{18:0}$ fatty acids, each in order of acid quantity. In regard to the composing pattern of carbon number of fatty acids, the dried purple laver o Mokpo, Hadong, and Jangrim contained $C_{16},\;C_{18}\;and\;C_{20}$ fatty acids with the identical distributional pattern, while that of Jangrim contained $C_{16},\;C_{18}\;and\;C_{17}$ fatty acids as major components. Dried Purple laver of Jangrim contained especially high amount of $C_{20:5}$ fatty acids.
Recently, sustainable production of biofuel using algal biomass is being pursued because of its enormous potential. First and foremost, securing superior strains to develop an efficient production system for algal biodiesel through screening or genetic improvement of microalgae is necessary. The genus of Botryococcus is regarded as one of the superior microalgae for biodiesel production due to its ability to accumulate high amounts of lipids and hydrocarbons. However, its low growth rate is a bottleneck for large-scale production and commercialization. The purpose of this study is to obtain indigenous Botryococcus strains which possess high lipid content and biomass productivity. The Botryococcus sp. was isolated from the Seobu Reservoir in Jeju Island and identified as Botryococcus sudeticus J2 by comparative analysis of 18s rRNA gene and ITS regions. The biomass productivity and lipid content of B. sudeticus J2 were 0.116 g $L^{-1}day^{-1}$ and 40.1% of dry wt., respectively. This was higher than the value of B. braunii UTEX 572, which is widely regarded as a superior strain among Botryococcus species. The relatively high growth rate of B. sudeticus J2 was achieved under a light intensity of 240 ${\mu}mol$ photons $m^{-2}s^{-1}$ with ambient air spargingwhen compared to 120 ${\mu}mol$ photons $m^{-2}s^{-1}$ with 2% $CO_2$ supply. In summary, it is likely that the isolated B. sudeticus J2 can be used for the mass cultivation and biodiesel production.
Jung, A Ram;Ahn, Sang Hyun;Jeong, Han Sol;Kim, Ki Bong
Journal of Physiology & Pathology in Korean Medicine
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v.33
no.2
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pp.102-108
/
2019
This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.
Kim, Yeon-Ju;Kim, Rae-Young;Park, Jae-Hee;Ju, Jong-Chan;Kim, Won-Tae;Chun, Soon-Sil
Journal of the Korean Society of Food Science and Nutrition
/
v.39
no.6
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pp.837-842
/
2010
The physicochemical properties of coarse semolina (CS), medium semolina (MS) and fine semolina (FS) were investigated to research method applied in noodles processing of Korean wheat semolina. Large particle (>250 ${\mu}m$) was over 75% in all semolinas, except for FS, and the particle distribution of MS and durum semolina (DS) was similar. Crude protein and crude ash were the highest in DS followed by CS>MS>FS. Crude lipid of DS was the lowest among samples and CS, MS and FS were not significantly different. L value was high in semolina with small particle distribution and starch damage was the lowest in DS followed by FS>MS. Amylose content was high in DS (29.80%) and FS (29.08%) with small particle distribution. Water binding capacity was the highest in DS, and FS showed the highest water binding capacity among Korean wheat samples. Solubility and swelling power were noticeably high in FS with low starch damage and small particle distribution. In scanning electron microscope (SEM), FS and MS showed distribution of separated fine particles of flours. From these results, the physicochemical properties of semolina showed many differences by grinding methods. FS should be applied in noodles processing through additional examination about characteristic of noodle making.
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