• Title/Summary/Keyword: lines detection

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Effects of $17{\beta}$-Estradiol and Estrogen Receptor Antagonists on the Proliferation of Gastric Cancer Cell Lines

  • Kim, Myung-Jin;Cho, Sung-Il;Lee, Kun-Ok;Han, Hyung-Joon;Song, Tae-Jin;Park, Seong-Heum
    • Journal of Gastric Cancer
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    • v.13 no.3
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    • pp.172-178
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    • 2013
  • Purpose: The aims of this study were as follow: 1) to de scribe the expression status of estrogen receptor-${\alpha}$ and -${\beta}$ mRNAs in five gastric carcinoma cell lines; 2) to evaluate in vitro the effects of $17{\beta}$-estradiol and estrogen receptor antagonists on the proliferation of the cell lines. Materials and Methods: Detection of estrogen receptor-${\alpha}$ and estrogen receptor-${\beta}$ mRNA in five human gastric cancer cell lines (AGS, KATO III, MKN28, MKN45 and MKN74) was made by the reverse transcription-polymerase chain reaction system. To evaluate the effect of $17{\beta}$-estradiol and estrogen receptor antagonists on the proliferation of gastric cancer cell line, the cell lines which expressed both es trogen receptors were chosen and treated with $17{\beta}$-estradiol and estrogen receptor antagonists (methyl-piperidino-pyrazole and pyrazolo [1,5-a] pyrimidine). Cell proliferation was assessed with the methylthiazol tetrazolium test. Results: Estrogen receptor-${\alpha}$ and estrogen receptor-${\beta}$ mRNAs were expressed in three (KATO III, MKN28 and MKN45) and all of the five gastric cancer cell lines, respectively. At higher concentrations, $17{\beta}$-estradiol inhibited cell growth of MKN28, MKN45 and KATO III cell lines. Neither estrogen receptor-${\alpha}$ nor estrogen receptor-${\beta}$ antagonist blocked the anti-proliferative effect of $17{\beta}$-estradiol. Conclusions: Our results indicate that estrogen receptor-${\beta}$ mRNAs are preferentially expressed in gastric cancers and also imply that hormone therapy rather than estrogen receptor blockers may be a useful strategy for the treatment of estrogen receptor-${\beta}$ positive gastric cancer. Its therapeutic significance in gastric cancer are, however, limited until more evidence of the roles of estrogen receptors in the gastric cancer are accumulated.

An Automatic Corona-discharge Detection System for Railways Based on Solar-blind Ultraviolet Detection

  • Li, Jiaqi;Zhou, Yue;Yi, Xiangyu;Zhang, Mingchao;Chen, Xue;Cui, Muhan;Yan, Feng
    • Current Optics and Photonics
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    • v.1 no.3
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    • pp.196-202
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    • 2017
  • Corona discharge is always a sign of failure processes of high-voltage electrical apparatus, including those utilized in electric railway systems. Solar-blind ultraviolet (UV) cameras are effective tools for corona inspection. In this work, we present an automatic railway corona-discharge detection system based on solar-blind ultraviolet detection. The UV camera, mounted on top of a train, inspects the electrical apparatus, including transmission lines and insulators, along the railway during fast cruising of the train. An algorithm based on the Hough transform is proposed for distinguishing the emitting objects (corona discharge) from the noise. The detection system can report the suspected corona discharge in real time during fast cruises. An experiment was carried out during a routine inspection of railway apparatus in Xinjiang Province, China. Several corona-discharge points were found along the railway. The false-alarm rate was controlled to less than one time per hour during this inspection.

Automatic Crack Detection on Pressed Panels Using Camera Image Processing with Local Amplitude Mapping (카메라 이미지 처리를 통한 프레스 패널의 크랙결함 검출)

  • Lee, Chang Won;Jung, Hwee Kwon;Park, Gyuhae
    • Journal of the Korean Society for Nondestructive Testing
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    • v.36 no.6
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    • pp.451-459
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    • 2016
  • Crack detection on panels during manufacturing process is an important step for ensuring the product quality. The accuracy and efficiency of traditional crack detection methods, which are performed by eye inspection, are dependent on human inspectors. Therefore, implementation of an on-line and precise crack detection is required during the panel pressing process. In this paper, a regular CCTV camera system is utilized to obtain images of panel products and an image process based crack detection technique is developed. This technique uses a comparison between the base image and a test image using an amplitude mapping of the local image. Experiments are performed in the laboratory and in the actual manufacturing lines to evaluate the performance of the developed technique. Experimental results indicate that the proposed technique could be used to effectively detect a crack on panels with high speed.

Photonic-Assisted Reactive-Near-Field Analysis of a 3 dB-Tapered Ka-Band Array Antenna

  • Lee, Dong-Joon;Kang, Jeong-Jin;Kang, No-Weon;Kim, Wan-Sik;Park, Wee-Sang;Rothwell, Edward J.;Whitaker, John F.
    • Journal of electromagnetic engineering and science
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    • v.10 no.1
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    • pp.18-24
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    • 2010
  • A Ka-band microstrip array antenna for wide-range detection of moving targets is analyzed through a photonicassisted reactive-near-field characterization technique. The antenna array employs a 3-dB-tapered feed network to suppress the sidelobe level while retaining a wide azimuth beamwidth for a wide detection range. The relative nearelectric field patterns of the array and its 3-dB-tapered feed lines have been measured using an electro-optic fieldmapping technique for minimally invasive millimeter-wave sensing. A number of typical limitations on the technique, involving bandwidth, low signal-modulation depth, and high laser-induced noise in high-frequency applications, have been overcome by suppressing the carrier portion of the optical interrogation beam.

Superconducting Strip Ion Detectors for Time-of-flight Mass Spectrometer

  • Zen, N.;Suzuki, K.;Shiki, S.;Ukibe, M.;Koike, M.;Casaburi, A.;Ejrnaes, M.;Cristiano, R.;Ohkubo, M.
    • Progress in Superconductivity
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    • v.14 no.2
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    • pp.92-95
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    • 2012
  • Superconducting detectors are promising as ion detectors for time-of-flight mass spectrometers (TOF MS). They can achieve mass-independent detection efficiency even for macromolecular bombardments, because output signals are produced through the deposited kinetic energy at ion impact instead of secondary electron emission that is the ion detection mechanism of conventional microchannel plate (MCP) detectors or secondary electron multipliers (SEM). Among the superconducting detectors, the superconducting strip ion detectors (SSIDs), which consist of several hundreds of superconducting lines with a width of a few hundreds nm and a thickness of a few tens of nm, have a fast response time of less than 1 ns. Inherently, the response time of SSIDs is determined by kinetic inductance, so that it was difficult to realize a fast SSID with a large detection area. However, we succeeded in realizing the detector size up to $5{\times}5mm^2$ without response time degradation by using a parallel configuration.

Digital Watermarking Technique for Images with Perspective Distortion

  • Chotikakamthorn, Nopporn;Yawai, Wiyada
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.1090-1093
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    • 2004
  • In this paper, a problem of geometrically distorted images is considered. In particular, the paper discusses the detection of a watermark from a photographed image of the watermarked picture. The image is possibly obtained by using a digital camera. This watermark detection problem is made difficult by various geometric distortions added to the original picture through the printing and photographing processes. In particular, the paper focuses on the geometric distortion due to a projective transformation, as part of a camera 3D-to-2D imaging process. It is well-known that a cross ratio of collinear points is invariant under a perspective projection. By exploiting this fact, a projective-invariant digital watermarking technique is developed. By detecting the picture's corners, and the image center point at the intersection of two main diagonal lines, predefined cross ratios are used to compute the watermark embedded locations. From those identified embedding pixel locations, a watermark can be detected by performing a correlation between a watermark pattern and the image over those pixels. The proposed method does not require an inverse transformation on the distorted image, thus simplifying the detection process. Performance of the proposed method has been analyzed through computer experiments

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A Study on Detection of Lane and Situation of Obstacle for AGV using Vision System (비전 시스템을 이용한 AGV의 차선인식 및 장애물 위치 검출에 관한 연구)

  • 이진우;이영진;이권순
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2000.11a
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    • pp.207-217
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    • 2000
  • In this paper, we describe an image processing algorithm which is able to recognize the road lane. This algorithm performs to recognize the interrelation between AGV and the other vehicle. We experimented on AGV driving test with color CCD camera which is setup on the top of vehicle and acquires the digital signal. This paper is composed of two parts. One is image preprocessing part to measure the condition of the lane and vehicle. This finds the information of lines using RGB ratio cutting algorithm, the edge detection and Hough transform. The other obtains the situation of other vehicles using the image processing and viewport. At first, 2 dimension image information derived from vision sensor is interpreted to the 3 dimension information by the angle and position of the CCD camera. Through these processes, if vehicle knows the driving conditions which are angle, distance error and real position of other vehicles, we should calculate the reference steering angle.

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Text Detection in Scene Images using spatial frequency (공간주파수를 이용한 장면영상에서 텍스트 검출)

  • Sin, Bong-Kee;Kim, Seon-Kyu
    • Journal of KIISE:Software and Applications
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    • v.30 no.1_2
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    • pp.31-39
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    • 2003
  • It is often assumed that text regions in images are characterized by some distinctive or characteristic spatial frequencies. This feature is highly intuitive, and thus appealing as much. We propose a method of detecting horizontal texts in natural scene images. It is based on the use of two features that can be employed separately or in succession: the frequency of edge pixels across vertical and horizontal scan lines, and the fundamental frequency in the Fourier domain. We confirmed that the frequency features are language independent. Also addressed is the detection of quadrilaterals or approximate rectangles using Hough transform. Since texts that is meaningful to many viewers usually appear within rectangles with colors in high contrast to the background. Hence it is natural to assume the detection rectangles may be helpful for locating desired texts correctly in natural outdoor scene images.

Utilization of Laser Range Measurements for Guiding Unmanned Agricultural Machinery

  • Jung, I. G.;Park, W. P.;Kim, S. C.;Sung, J. H.;Chung, S. O.
    • Agricultural and Biosystems Engineering
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    • v.2 no.2
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    • pp.69-74
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    • 2001
  • Detection of operation lines in farm works, object recognition and obstacle avoidance are essential pre-requisite technologies for unmanned agricultural machinery. A CCD camera, which has been largely used for these functions, is expensive and has difficulty in real-time signal processing. In this study, a laser range sensor was selected as the guiding vision for unmanned agricultural machinery such as a tractor. To achieve this capability, algorithms for distance measurement, signal filtering, object recognition, and obstacle avoidance were developed. Computer simulations were carried out to evaluate performance of the algorithms. Experiments were also conducted with various materials and shapes, Laser beam lost its intensity for poor reflective materials, resulting in less range value than actual, so a compensation technique was considered to be necessary. Object detection system was fabricated on an agricultural tractor and the performance was evaluated. As test result for obstacle detection and avoidance in field, to detect and avoid obstacle for path finding with guiding system for unmanned agricultural machinery was enable.

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Detection of Fusarium Species by Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibody

  • Kwak, Bo-Yeon;Kwon, Byung-Joon;Kweon, Chang-Hee;Shon, Dong-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.13 no.5
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    • pp.794-799
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    • 2003
  • Enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of Fusarium species, known as harmful fungi in food. One of the hybridoma cell lines (lB8) which produced a monoclonal antibody (Mab) specific to Fusarium extracellular polysaccharide (EPS) was screened and the Mab was produced and purified. A detection limit of the sandwich ELISA against F. moniliforme EPS was $0.001\;\mu\textrm{g}/ml$ in the standard curve. Among the 59 strains tested, most Fusarium species showed hight reactivity with Mab lB8, even when the culture broths were diluted 100,000 times. On the other hand, the other genera, except A. versicolor and Trichoderma viride, had no reactivity. When 1 to $100\;\mu\textrm{g}$ of F. moniliforme EPS was spiked into rice, potato, and mandarine orange, the average recoveries were 151%, 84%, and 94%, respectively, determined by sandwich ELISA. The correlation coefficients between the EPS levels determined by sandwich ELISA and the dry mycelial weight of the liquid culture of F. moniliforme, as well as between the EPS and colony forming unit in solid culture of potato, were 0.97 and 0.91, respectively.