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The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit (뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정)

  • Choi, Sunmi;Kim, Giyoung;Om, Aeson;Moon, Jihea;Park, Saetbyeol;Lee, Sangdae;Kim, Hyuk Joo
    • Korean Journal of Agricultural Science
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    • v.39 no.4
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    • pp.613-618
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    • 2012
  • This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.

Improving the Accuracy of the Tapped Delay Time-to-Digital Converter Using Field Programmable Gate Array (Field-Programmable Gate Array를 사용한 탭 딜레이 방식 시간-디지털 변환기의 정밀도 향상에 관한 연구)

  • Jung, Do-Hwan;Lim, Hansang
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.9
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    • pp.182-189
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    • 2014
  • A tapped delay line time-to-digital converter (TDC) can be easily implemented using internal carry chains in a field-programmable gate array, and hence, its use is widespread. However, the tapped delay line TDC suffers from performance degradation because of differences in the delay times of dedicated carry chains. In this paper, a dual edge measurement method is proposed instead of a typical step signal to the delay cell to compensate for the performance degradation caused by wide-delay cells in carry chains. By applying a pulse of a fixed width as an input to the carry chains and using the time information between the up and down edges of the signal pulse, the timing accuracy can be increased. Two dedicated carry chain sites are required for the dual edge measurements. By adopting the proposed dual edge measurement method, the average delay widths of the two carry chains were improved by more than 35%, from 17.3 ps and 16.7 ps to 11.2 ps and 10.1 ps, respectively. In addition, the maximum delay times were improved from 41.4 ps and 42.1 ps to 20.1 ps and 20.8 ps, respectively.

Studies on the Micelle Formation of Nonionic Surfactant(1) -1NMR Self-Diffusion and Proton Relaxation of Polyoxyethylene Alkyl Ether- (계면활성제 수용액의 미셀형성(제1보) - Polyoxyethylene Alkyl Ether의 자기확산과 프로톤 이완 -)

  • Choi, Seung-Ok;Jeong, Hwan-Kyeong;Lee, Jin-Hee;Nam, Ki-Dae
    • Applied Chemistry for Engineering
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    • v.9 no.6
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    • pp.822-828
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    • 1998
  • Binary system of water and polyoxyethylene dodecyl ether, $C_{12}H_{25}(OCH_2CH_2)nOH$, have been studied by $^1H$ NMR techniques. For n=5($C_{12}EO_5$) and n=8($C_{12}EO_8$), the self-diffusion coefficients of nonionic surfactants in the isotropic phase($L_1$) have been measured by using pulsed field gradient technique for a range of temperature and concentrations. In addition the line widths of the different proton signals have been monitored, and samples of some liquid crystalline characteristic were also studied. Dramatic Broadening of the methylene signals of the alkyl($C_{12}H_{25}$) chain is observed as the hexagonal liquid crystalline phase is approached in the $C_{12}EO_5-$water system, while only small broadening is observed in the $C_{12}EO_8-$water system. It was shown that there was a growth of $C_{12}EO_5$ micelles to rods with increasing concentrations, while the $C_{12}EO_8-$ micelles at low temperature remain small in the concentration range. The self-diffusion coefficients of the surfactants decrease rapidly with increasing concentration until a minimum is reached after which there is slow increase. The location of the minimum point occurs at lower concentrations the temperature is close to the cloud point, where the system separate into two isotropic phase. In the line width studies, broadening is found at a certain temperature interval when the concentration is increased in the $C_{12}EO_5$ system. The results indicate that the surfactant aggregates grow in size at the cloud point is approached. The aggregates seem to be flexible and probably not to be of a definite shape close to the cloud point. In the $C_{12}EO_8$ system, the micelles are much less affected by an increase in temperature and micellar growth can't be unambiguously established. The methylene signals of the ethylene oxide moieties consistantly show narrower $^1H$ signals, showing that in the aggregates they are less ordered than the chain methylenes. The various changes in aggregate size and shape are correlated with the stability ranges of the isotropic and liquid crystalline phases according to phase diagrams from the literature. Both aggregate size and phase structure are in qualitative agreement with concentration based on the effective shape of the molecules at different temperature and concentration.

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MONITORING OBSERVATIONS OF H2O AND SiO MASERS TOWARD POST-AGB STARS

  • Kim, Jaeheon;Cho, Se-Hyung;Yoon, Dong-Hwan
    • Journal of The Korean Astronomical Society
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    • v.49 no.6
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    • pp.261-288
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    • 2016
  • We present the results of simultaneous monitoring observations of $H_2O$ $6_{1,6}-5_{2,3}$ (22GHz) and SiO J=1-0, 2-1, 3-2 maser lines (43, 86, 129GHz) toward five post-AGB (candidate) stars, using the 21-m single-dish telescopes of the Korean VLBI Network. Depending on the target objects, 7 - 11 epochs of data were obtained. We detected both $H_2O$ and SiO maser lines from four sources: OH16.1-0.3, OH38.10-0.13, OH65.5+1.3, and IRAS 19312+1950. We could not detect $H_2O$ maser emission toward OH13.1+5.1 between the late OH/IR and post-AGB stage. The detected $H_2O$ masers show typical double-peaked line profiles. The SiO masers from four sources, except IRAS 19312+1950, show the peaks around the stellar velocity as a single peak, whereas the SiO masers from IRAS 19312+1950 occur above the red peak of the $H_2O$ maser. We analyzed the properties of detected maser lines, and investigated their evolutionary state through comparison with the full widths at zero power. The distribution of observed target sources was also investigated in the IRAS two-color diagram in relation with the evolutionary stage of post-AGB stars. From our analyses, the evolutionary sequence of observed sources is suggested as OH65.5+1.3${\rightarrow}$OH13.1+5.1${\rightarrow}$OH16.1-0.3${\rightarrow}$OH38.10-0.13, except for IRAS 19312+1950. In addition, OH13.1+5.1 from which the $H_2O$ maser has not been detected is suggested to be on the gateway toward the post-AGB stage. With respect to the enigmatic object, IRAS 19312+1950, we could not clearly figure out its nature. To properly explain the unusual phenomena of SiO and $H_2O$ masers, it is essential to establish the relative locations and spatial distributions of two masers using VLBI technique. We also include the $1.2-160{\mu}m$ spectral energy distribution using photometric data from the following surveys: 2MASS, WISE, MSX, IRAS, and AKARI (IRC and FIS). In addition, from the IRAS LRS spectra, we found that the depth of silicate absorption features shows significant variations depending on the evolutionary sequence, associated with the termination of AGB phase mass-loss.

Study on Mathematical Method of Radiation Heat Transfer for Estimating Width of Firebreak in Surface Fire (복사열전달 수치해석을 통한 지표화 방화선 구축 폭 산정에 관한 연구)

  • Kim, Dong-Hyun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.10 no.6
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    • pp.59-64
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    • 2010
  • Building a firebreak against surface forest fire is a typical indirect suppression method that stops spread of flame by removing surface fuel, such as fallen leaves and bushes. In the sense of fire dynamic, building a firebreak is to set a section which will block thermal energy from igniting on virgin fuel. This study suggests and evaluates a calculation method for width of firebreak against surface fire for variant wind and slope conditions by applying the Point Source Model (PSM) to fallen leaves of Pinus densiflora. Width of firebreak was measured based on the distance the threshold radiant heat igniting Pinus densiflora fallen leaves at the heat flux of $4.9\;kW/m^2$ reaches. As a result, at the wind velocity of 0~5 m/s and on the slope of $0{\sim}50^{\circ}$, the appropriate width of a firebreak was 0.35~0.65 m for the mean flame height and 0.75~1.05 m for the maximum flame height. Accordingly, considering the factor of safety, the most appropriate width of a firebreak is 1.05 m based on the maximum flame height. Additional comparative analyses through experiments and field surveys are deemed necessary to determine appropriate widths of firebreak for different types of surface fuel.

Simulation Study of a Large Area CMOS Image Sensor for X-ray DR Detector with Separate ROICs (센서-회로 분리형 엑스선 DR 검출기를 위한 대면적 CMOS 영상센서 모사 연구)

  • Kim, Myung Soo;Kim, Hyoungtak;Kang, Dong-uk;Yoo, Hyun Jun;Cho, Minsik;Lee, Dae Hee;Bae, Jun Hyung;Kim, Jongyul;Kim, Hyunduk;Cho, Gyuseong
    • Journal of Radiation Industry
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    • v.6 no.1
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    • pp.31-40
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    • 2012
  • There are two methods to fabricate the readout electronic to a large-area CMOS image sensor (LACIS). One is to design and manufacture the sensor part and signal processing electronics in a single chip and the other is to integrate both parts with bump bonding or wire bonding after manufacturing both parts separately. The latter method has an advantage of the high yield because the optimized and specialized fabrication process can be chosen in designing and manufacturing each part. In this paper, LACIS chip, that is optimized design for the latter method of fabrication, is presented. The LACIS chip consists of a 3-TR pixel photodiode array, row driver (or called as a gate driver) circuit, and bonding pads to the external readout ICs. Among 4 types of the photodiode structure available in a standard CMOS process, $N_{photo}/P_{epi}$ type photodiode showed the highest quantum efficiency in the simulation study, though it requires one additional mask to control the doping concentration of $N_{photo}$ layer. The optimized channel widths and lengths of 3 pixel transistors are also determined by simulation. The select transistor is not significantly affected by channel length and width. But source follower transistor is strongly influenced by length and width. In row driver, to reduce signal time delay by high capacitance at output node, three stage inverter drivers are used. And channel width of the inverter driver increases gradually in each step. The sensor has very long metal wire that is about 170 mm. The repeater consisted of inverters is applied proper amount of pixel rows. It can help to reduce the long metal-line delay.

Identification of shear layer at river confluence using (RGB) aerial imagery (RGB 항공 영상을 이용한 하천 합류부 전단층 추출법)

  • Noh, Hyoseob;Park, Yong Sung
    • Journal of Korea Water Resources Association
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    • v.54 no.8
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    • pp.553-566
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    • 2021
  • River confluence is often characterized by shear layer and the associated strong mixing. In natural rivers, the main channel and its tributary can be separated by the shear layer using contrasting colors. The shear layer can be easily observed using aerial images from satellite or unmanned aerial vehicles. This study proposes a low-cost identification method extracting geographic features of the shear layer using RGB aerial image. The method consists of three stages. At first, in order to identify the shear layer, it performs image segmentation using a Gaussian mixture model and extracts the water bodies of the main channel and tributary. Next, the self-organizing map simplifies the flow line of the water bodies into the 1-dimensional curve grid. After that, the curvilinear coordinate transformation is performed using the water body pixels and the curve grid. As a result, the shear layer identification method was successfully applied to the confluence between Nakdong River and Nam River to extract geometric shear layer features (confluence angle, upstream- and downstream- channel widths, shear layer length, maximum shear layer thickness).

3D Face Dimensions and New Fit Test Panels for the Labor Population Using Respirators in South Korea (호흡보호구 사용 노동인구의 3차원 얼굴사이즈 특징 및 한국형 밀착도 검사 패널 개발)

  • Jung-Keun Park;Se-Dong Kim;Hyoun-Min Cho
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.33 no.2
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    • pp.247-264
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    • 2023
  • Objectives: The study was to examine the features of three-dimensional(3D) face dimensions and to develop fit test panels for the labor population using respirators in South Korea. Methods: This study was part of the first-year work of a two-year-project conducted at Occupational Safety and Health Research Institute in 2021. After 3D head dimensions data were collected from Size Korea Center managing Sixth Size Korea databases, 3D face dimensions DB for the South Korean labor population was established for 21 items of face dimensions in line with the ISO/TS 16976-2 and KS A ISO 15535. With the South Korean labor population 3D face dimensions DB, in accordance with the ISO/TS 16976-2, the descriptive statistics of 3D face dimensions were calculated and two fit test panels were developed. Results: A total of 2,752 subjects were finally determined and they were 52.9% for male, 41.2% for the highest age-group of 15-29, and residents in the capital area. Mean and standard deviation were obtained for each of the 21 3D face dimensions items for the South Korean labor population. Among the items, male and female face widths were 137.6±5.7 mm, 133.2±5.0 mm, respectively. Male and female face lengths were 116.6±7.0 mm, 107.8±6.8 mm, respectively. Two new South Korean fit test panels, a bivariate test panel and a principal component analysis test panel, were developed using the 3D face dimensions DB as well. Conclusions: Using the 3D face dimensions DB, the mean and standard deviation were featured for each of the 21 items and also the two fit test panels were newly developed in the study. It is suggested that the study outputs should be utilized practically and effectively in selection, use, and management of respirators at work, expecting that respiratory protection can be furthermore improved for respirator users including labor population across the country.

Dose distribution at junctional area for head and neck radiotherapy (두경부 방사선치료시 접합 조사면의 선량분포)

  • 김정기;김기환;오영기;김진기;정동혁;신교철;양광모;조문준;박인규
    • Progress in Medical Physics
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    • v.12 no.2
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    • pp.161-169
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    • 2001
  • For the head and neck radiotherapy, the technique of half beam using independent collimator is very useful to avoid overlapping of fields particularly when the lateral neck fields are placed adjacent to anterior supraclavicular field. Also abutting photon field with electron field is frequently used for the irradiation of posterior neck when tolerable dose on spinal cord has been reached. Using 6 MV X-ray and 9 MeV electron beams of Clinac1800(Varian, USA) linear accelerator, we performed film dosimetry by the X-OMAT V film of Kodak in solid water phantom and the dose distribution at beam center of 2 half beams further examined according to depths(0 cm, 1.5 cm, 3 cm, 5 cm) for single anterior half beam and anterior/posterior half beam. The dose distribution to the junction line between photon and electron fields was also measured. For the single anterior half beam, the absorption doses at 0.3 cm, 0.5 cm and 1 cm distances from beam center were 88%, 93% and 95% of open beam, respectively. In the anterior/posterior half beams, the absorption doses at 0.3 cm, 0.5 cm and 1 cm distances from beam center were 92%, 93% and 95% of open beam, respectively At the junction line between photon and electron fields, hot spot was developed on the side of the photon field and a cold spot was developed on that of the electron field. The hot spot in the photon side was developed at depth 1.5 cm with 7 mm width. The maximum dose of hot spot was increased to 6% of reference doses in the photon field. The cold spot in the electron side was developed at all measured depths(0.5 cm-3 cm) with 1-12.5 mm widths. The decreased dose in the cold spot was 4.5-30% of reference dose in the electron field. With above results, we concluded that when using electron beam or independent jaw for head and neck radiotherapy, the hot and cold dose area should be considered as critical point.

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Dose distribution at junctional area abutting X-ray and electron fields (X-선과 전자선의 인접조사에서 접합 조사면에서의 선량분포)

  • Yang, Kwang-Mo
    • The Journal of Korean Society for Radiation Therapy
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    • v.16 no.1
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    • pp.91-99
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    • 2004
  • Purpose : For the head and neck radiotherapy, abutting photon field with electron field is frequently used for the irradiation of posterior neck when tolerable dose on spinal cord has been reached. Materials and methods : Using 6 MV X-ray and 9 MeV electron beams of Clinac1800(Varian, USA) linear accelerator, we performed film dosimetry by the X-OMAT V film of Kodak in solid water phantom according to depths(0 cm, 1.5 cm, 3 cm, 5 cm). 6 MV X-ray and 9 MeV electron(1Gy) were exposes to 8cm depth and surface(SSD 100cm) of phantom. The dose distribution to the junction line between photon($10cm{\times}10cm$ field with block) and electron($15cm{\times}15cm$ field with block) fields was also measured according to depths(0 cm, 0.5 1.5 cm, 3 cm, 5 cm). Results : At the junction line between photon and electron fields, the hot spot was developed on the side of the photon field and a cold spot was developed on that of the electron field. The hot spot in the photon side was developed at depth 1.5 cm with 7 mm width. The maximum dose of hot spot was increased to $6\%$ of reference doses in the photon field. The cold spot in the electron side was developed at all measured depths(0.5 cm-3 cm) with 1-12.5 mm widths. The decreased dose in the cold spot was $4.5-30\%$ of reference dose in the electron field. Conclusion : When we make use of abutting photon field with electron field for the treatment of head and neck cancer we should consider the hot and cold dose area in the junction of photon and electron field according to location of tumor.

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