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Dietary Squid Liver Oil and Soybean Oil Ratio on Growth and Body Composition of Korean Rockfish Sebagtes schlegeli (배합사료에 오징어간유와 대두유 첨가비가 성장기 조피볼락의 성장과 체성분에 미치는 효과)

  • 이상민;이종윤;전임기
    • Journal of Aquaculture
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    • v.13 no.3
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    • pp.207-213
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    • 2000
  • Five diets, containing different levels (0.3-1.5%) of n-3 highly unsaturated fatty acids (n-3HUFA) adjusted by adding different ratio of squid liver oil and soybean oil to 8% lipid, were fed to the rockfish (130 g) for 8 weeks. Mean weight gain and feed efficiency were lowest in the fish fed the diet containing 0.3% n-3HUFA. These values improved with increasing squid liver oil, and showed linear relation up to 0.6% n-3HUFA, Using the brocken line model, the dietary n-3HUFA requirement was estimated as about 0.9 % for optimal weight gain of the fish. Crude lipid levels of the liver in 0.3-0.6%) n-3HUFA diets were significantly higher than in the 1.5% n-3HUFA diet (P<0.05). Fatty acid composition of polar lipid in the liver were directly affected by dietary lipid sources. The level of n-3HUFA of polar lipid in the liver increased with dietary n-3HUFA levels, although 18:2n-6 content decreased. Hence the n-3HUFA requirement of a growing rockfish is 0.6-0.9% of diet.

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Use of RAPD-PCR(Random Amplified Polymorphic DNA-Polymerase Chain Reaction) Method for a Detection of Pathogenic Listeria monocytogenes (RAPD-PCR(Random Amplified Polymorphic DNA - Polymerase Chain Reaction) 방법을 이용한 Listeria monocytogenes의 검색)

  • Park Bum-Joon;Sihn Eon-Hwan
    • The Korean Journal of Food And Nutrition
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    • v.17 no.3
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    • pp.254-259
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    • 2004
  • Rapid detection of foodbome pathogens is becoming increasingly important. The requirement for faster, more reliable tests has lead to the development of a wide range of rapid methods. Among these methods, the use of systems based on nucleic acid based detection has been increasing since they offer advantages of reduction in test time and more reliable detection or identification. Random Amplification Polymorphic DNA(RAPD) method has been used to fingerprint foodbome microorganisms; Listeria monocytogenes. In this study, 10-mer primer OPG-13(5'-CTCTCCGCCA-3') was used to generate RAPD-PCR for detection of pathogenic L. monocytogenes of Listeria spp. Among 20 primers tested, OPG-13 showed on acceptable result for the differentiation of a pathogenic Listeria from non-pathogenic microorganisms. Pathogenic Listeria, L. monocytogenes(ATCC 15313, 19111, 19112, 19113) showed two bands for 700 bp and 1,500 bp while non-pathogenic bacteria, L. ivanovii, L. grayi, L. murrayi, L. innocua, L. welshimeri, and L. seeligeri had only one band sizing from 2,000 to 2,300 bp. This RAPD method proved to be a valuable to gain important information on sources of pathogenic bacteria in food industry.

Effect the I-T curve and electrical characteristic of fuse elements by plated tin thickness (주석 도금 두께에 따른 퓨즈 가용체의 I-T 커브 및 전기적 특성의 영향)

  • Jin, Sang-Jun;Kim, Eun-Min;Youn, Jae-Seo;Lee, Ye-Ji;Noh, Seong-Yeo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.80-87
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    • 2018
  • In recent years, due to the spread of various renewable energy power sources and the pursuit of high efficiency and low-power consumption, not only trends in the electric power industry but also the consumption, control methods, and characteristics are diversified. However, in this diversified electric power industry, the fuse (which is the core part responsible for safety) has not developed significantly in classical operation mode, and thus, fires continue to occur. In this paper, the effects of low melting-point metal plating and high melting-point metal plating on operating characteristics and IT curve movement of the fuse are investigated in a cartridge fuse, which is a classic fuse manufacturing method. The effects of plating on the thickness of the fuse are investigated, and various operating characteristics of the fuse are implemented. In addition, it is suggested that the plating of the low melting-point metal moves the rated current line of the fuse to a low rating, and moves operating characteristics to characteristics of delay operation. It is possible to design various operating characteristics using this characteristic.

Mass Production of Gain-of-Function Mutants of Hair Roots in Ginseng (기능획득 돌연변이 인삼 모상근의 대량생산)

  • Ko, Suk-Min;In, Dong-Soo;Chung, Hwa-Jee;Choi, Dong-Woog;Liu, Jang-Ryol
    • Journal of Plant Biotechnology
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    • v.34 no.4
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    • pp.285-291
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    • 2007
  • This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.

Genetic Analysis on the Bacterial Blight Resistance Gene from a Wild Relative, Oryza minuta (야생벼 Oryza minuta에서 유래한 수원506호의 흰잎마름병 저항성유전자에 대한 고찰)

  • Jeung, Ji-Ung;Roh, Tae-Hwan;Kang, Kyung-Ho;Shin, Young-Seop;Kim, Yeon-Gyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.124-133
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    • 2011
  • Bacterial blight (BB), cuased by the vascular pathogen Xanthomonas oryzae pv. oryzae, is one of the major threats in rice fields worldwide. In Korea, two resistance genes against BB, Xa1 and Xa3 had been intensively used for developing high quality japonica rice cultivars. Those traditional resistance sources have being rapidly ified by the adopting of BB pathogen through mutations of the corresponding avr-genes, such as K3a exhibiting high compatibility to both Xa1 and Xa3. To expanding genetic resource against BB in Korea, the Suweon506, an introgression line between a Korean japonica cultivar, Hwaseong and a wild relative, Oryza minuta, was be subjected for genetic analysis owing to the BB resistance. Through association analyses between the pathotyping and genotyping results for each $F_2$ progenies, derived from a cross between Suweon506 and a Tongil type cultivar, Milyang23, a major resistant dominant gene is localized on the subterminal region of rice chromosome 4, where at least three BB resistancde genes, Xa1, Xa2, and Xa22, were reported previously.

A Study on the Common Features of Western Clothing Style and the Style of Korean clothing - Focusing on the Three Kingdom and Unified Shilla era - (우리 나라 복식(服飾)과 서역복식간(西域服飾間)의 공통성(共通性)에 관(關)한 연구(硏究) - 삼국시대(三國時代), 통일신라시대( 統一新羅時代)를 중심(中心)으로 -)

  • Jeun, Jee-Eun;Shim, Hwa-Jin
    • Journal of Fashion Business
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    • v.5 no.4
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    • pp.127-143
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    • 2001
  • This thesis looks into the characteristics and compares the clothing styles between the Three Kingdom to the Unified Shilla era. The purpose of this study is to see what common themes exists between the foundation of Korean clothing and how it has evolved and through see to what extent the Chinese influence has been to Korean clothing culture. Also by explaining the originality and creativity of Korean clothing, we will be able to make clear the status of Korean culture. These findings include the following; 1. The Korean strait line Gik-ryong Kyo-im can also be found at the Gochang region in the west. 2. The Ban-ryong-ui, worn by the aristocrats of the Three kingdom and Unified Shilla era, began to appear from the nobles from the Nam-Buk cho(South-north era). This combined with a Bokdu(headwear) became a part of casual dress wear. 3. Go(pants) became a traditional part of clothing for Unified Shilla and the west. 4. Ban-bi and Bae-dang, all forms of Bansu-ui ( short sleeve), came from Chinas Kucha and Hotan and came to Korea. 5. Chinas Pae-baek and Pae-za (Shawl) which is the same as Unified Shillas Peo was also to be found to have come from the west. 6. The way a different color line was added to clothing both can be found in Korean and western clothing. Similarities to material used are; First, expensive wool was used and mostly originated from the west. From far away Rome came the O-saek-gae (Five-color wool). Secondly, Kong-gak me (peacock feather) and Bi-chi mo were all originated from the west. Third, Sil-sil is stated in the Sam-kuk Sa-ki (Three Kingdom records) as being prohibited to be worn by Jin-gol women (Highest nobility during Shilla era) as headwear or comb (pin) or as a comb(pin) for yuk-doo poom ( 6th class nobility) women. Suggesting that it existed and other sources tell us that it came from Tashkent region of Russia and was a jewelry of some sort. From what we have seen above, we can see the similarities between Korean and western clothing cultures and that these interactions not only occurred with China but with many other nations. We can see that our ancestors were creative and original that when importing foreign cultures that they transformed them into a Korean style. That these foreign cultures were transformed into our own style is good evidence to these facts.

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A Study of Parallel Test Among Three ADVIA 2120 System (3대의 ADVIA 2120 System 평행시험에 대한 연구)

  • Chang, Sang-Wu;Cho, Eun-Hae;Kim, Nam-Yong;Chu, Kyung-Bok;Lee, Suk-Jong;Hong, Sung-No;Oh, Jong-Do
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.1
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    • pp.16-21
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    • 2006
  • Parallel testing means ordering a number of tests at the same time so abnormalities in any of the tests can be found quickly and used in making the diagnosis. This is a good medical strategy to eliminate diseases and it is relatively inexpensive if all the tests are potential sources of information and performed on the same analyzer. In regression, the equation for the straight line is recast as y = bx + a. This change in terminology leads to confusion. Here a is the y-intercept or constant and b is the coefficient or slope of the line. A few more words of caution about regression - as in all of statistics there are certain assumptions: the x value is a true measure, both X and Y distributions are normal, and homoscedasticity, i.e., the variance of y is the same for each value of x. In this study the linearity classification made by different scientists were always in agreement. Typical examples of curves that were considered linear are presented in Fig. 1-5. Because these automated procedures values were usually within five percent of each other the curvature could be easily detected. The plot of the WBC, RBC, hemoglobin, hematocrit and platelet concentrations from approximately 74.4 to $0{\times}10^3/{\mu}L$ and $80.4-0{\times}10^3/{\mu}L$, $5.6-0{\times}10^6/{\mu}L$ and $6.1-0{\times}1106/{\mu}L$, 18.3-0 g/dL and 19.0-0 g/dL, 54.1-0% and 56.8-0% and 642.0 to $0.03{\times}10^3{\mu}L$ and $754.0-0{\times}10^3/{\mu}L$ on the ADVIA 2120 C Versus and A and B typical of an acceptable linear study as shown in Fig. 1-5. The grand mean of R2, intercept and slope is 0.99898, 0.99459 and 1.54626.

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Isolation, Purification, and Characterization of Five Active Diketopiperazine Derivatives from Endophytic Streptomyces SUK 25 with Antimicrobial and Cytotoxic Activities

  • Alshaibani, Muhanna M.;MohamadZin, Noraziah;Jalil, Juriyati;Sidik, Nik Marzuki;Ahmad, Siti Junaidah;Kamal, Nurkhalida;Edrada-Ebel, RuAngelie
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1249-1256
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    • 2017
  • In our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from endophytic Streptomyces SUK 25 afforded five active diketopiperazine (DKP) compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate their bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., and their cytotoxic activities against the human hepatoma (HepaRG) cell line. The production of secondary metabolites by this strain was optimized through Thornton's medium. Isolation, purification, and identification of the bioactive compounds were carried out using high-performance liquid chromatography, high-resolution mass liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance, and cryopreserved HepaRG cells were selected to test the cytotoxicity. The results showed that endophytic Streptomyces SUK 25 produces four active DKP compounds and an acetamide derivative, which were elucidated as $cyclo-({\text\tiny{L}}-Val-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Leu-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Phe-{\text\tiny{L}}-Pro)$, $cyclo-({\text\tiny{L}}-Val-{\text\tiny{L}}-Phe)$, and N-(7-hydroxy-6-methyl-octyl)-acetamide. These active compounds exhibited activity against methicillin-resistant S. aureus ATCC 43300 and Enterococcus raffinosus, with low toxicity against human hepatoma HepaRG cells. Endophytic Streptomyces SUK 25 has the ability to produce DKP derivatives biologically active against some MDR bacteria with relatively low toxicity against HepaRG cells line.

The Roles of Self-Expression and Identification on the Personal Community Commitment (개인 커뮤니티 몰입에 대한 자아표현 및 동일시의 역할)

  • Choi, Nak Hwan;Lee, Chang Won
    • Asia Marketing Journal
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    • v.9 no.3
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    • pp.117-149
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    • 2007
  • It can be explained by congruity theory as a process that consumers engage in a matching process to identify personal community that is congruent with their self-images to find the identification between the self and the personal community. Personal community cues that evoke certain images are viewed as activating similar beliefs about the self (e.g., high status). Individuals prompt a comparison process to determine whether the personal community and self-image are congruent and imagine prototypical users of the personal community and select ones that maximize similarity to their actual or desired self-concept. Identity is devided into personal identity and social identity. Consumers are likely to be influenced by both personal identity and social identity. In this article the influencing factors of the commitment to on-line personal community are explored by the sources of both personal identification and social identification. The results are as follows. The maintenance expression and enhancement expression of personal self influence the level of personal identification positively and the maintenance expression and enhancement expression of social self influence the level of social identification positively. The level of both social and personal identification positively influence the commitment to on-line personal community which gives positive responses to the source enterprise that allows the cyberspace and the other benefits to be used.

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Experiment of KOMPSAT-3/3A Absolute Radiometric Calibration Coefficients Estimation Using FLARE Target (FLARE 타겟을 이용한 다목적위성3호/3A호의 절대복사 검보정 계수 산출)

  • Kyoungwook Jin;Dae-Soon Park
    • Korean Journal of Remote Sensing
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    • v.39 no.6_1
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    • pp.1389-1399
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    • 2023
  • KOMPSAT-3/3A (K3/K3A) absolute radiometric calibration study was conducted based on a Field Line of sight Automated Radiance Exposure (FLARE) system. FLARE is a system, which has been developed by Labsphere, Inc. adopted a SPecular Array Radiometric Calibration (SPARC) concept. The FLARE utilizes a specular mirror target resulting in a simplified radiometric calibration method by minimizing other sources of diffusive radiative energies. Several targeted measurements of K3/3A satellites over a FLARE site were acquired during a field campaign period (July 5-15, 2021). Due to bad weather situations, only two observations of K3 were identified as effective samples and they were employed for the study. Absolute radiometric calibration coefficients were computed using combined information from the FLARE and K3 satellite measurements. Comparison between the two FLARE measurements (taken on 7/7 and 7/13) showed very consistent results (less than 1% difference between them except the NIR channel). When additional data sets of K3/K3A taken on Aug 2021 were also analyzed and compared with gain coefficients from the metadata which are used by current K3/K3A, It showed a large discrepancy. It is assumed that more studies are needed to verify usefulness of the FLARE system for the K3/3A absolute radiometric calibration.