• The Korean Journal of Food And Nutrition
• /
• v.24 no.1
• /
• pp.94-100
• /
• 2011

This study identified the formula of the antioxidant substance separated from the ethyl acetate and the butanol extract and tested the antioxidant properties with the electron donating ability(EDA). Each phase with the fractionated methanol extract from mulberry leaf was screened in advance for the antioxidant substance with EDA. As the result, activity appeared in the ethyl acetate and butanol phase and the antioxidant component was separated. As the consequence, 2 components from the ethyl acetate phase and 1 from the butanol phase were separated, among which the structures of the components from ethyl acetate were determined by wogonin and linarin, whereas the structure of the component from the butanol phase was determined by pectolinarin. In the screening of antioxidant activity by the scavenging effect of the DPPH radical, the wogonin and linarin components from ethyl acetate phase showed more powerful antioxidant property than the component from butanol. The results from this study indicate that the chemical compound separated from the ethyl acetate extract has more powerful antioxidant property than the one separated from the butanol extract. The components separated from the ethyl acetate extract were wogonin and linarin, which are flavonoids, whereas the component from butanol was pectolinarin. Therefore, this study suggested that the feasibility of mulberry leaf as a functional food additive and its value as a natural antioxidant is very high.

• YAKHAK HOEJI
• /
• v.11 no.1_2
• /
• pp.7-16
• /
• 1967

A yellowish microneedles, $C_{28}$ H$_{32}$ $O_{14}$ ${\cdot}$ I$_{1}$/$_2$, H$_{2}$O, m.p.262-$4^{\circ}$ , [${\alpha}$$_{D}^{20}$= -71,$43^{\circ}$(C = 0.42, pyridine), its acetate m.p.123-5.deg., were obtained in 0.3% yield from the leaves of Chrysanthemum sibiricum F$_{ISCHER}$. This substance is insoluble in water and the usual organic solvents except pyridine and ethylene glycol and, is not decomposed by dilute mineral acids but undergoes decomposition on being boiled in 60% H$_{2}$SO$_{4}$ or 35% HCl, giving one moel each of acacetin, glucose and rhamnose. It was not hydrolysed with a rhamnodiastase preparation obtained from the seeds of Rhamnus koraiensis. After permethylation of it, the uncrystallized product was hydrolysed and apigenin-5,4'-dimethyl ehter, m.p.$262^{\circ}$ was obtained, indicating that the disaccharide residue is at the 7 position of acacetin. Partial hydrolysis of this acacetin-7-rhamnoglucoside in cyclohexanol with formic acid gave acacetin-7-glucoside, m.p.246.deg. and rutinose, identifying them with authentic specimen on a paper chromatography. It was thus identified as linarin(acacetin-7-rutinoside) by means of mixed fusion, of paper partition chromatography and of its derivatives. Zemplen and Bognar suggested that the glucosidic linkage of linarin is .betha. by means of synthesis of this substance. But there is no evidence whether it is hydrolysed by emulsin or maltase or not. Linarin itself was not hydrolysed by an emulsin existing in the seed of Apricot or a maltase, but acacetin-7-glucoside(tilianin) which obtained from linarin gave acacetin and glucose on hydrolysis with the same emulsin and accordingly the glucosidic linkages of linarin and tilianin are thus regarded as ${\beta}$.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.1
• /
• pp.1-4
• /
• 2003

Two antioxidative compounds in Chrysanthemum boreale were isolated by a bioassay using a 2, 2-diphenyl-1-picrydrazyl(DPPH) free radical. They were identified as apigenin, linarin(acacetin-7-O-rutinoside) on the basis of $^1H\;and\;^{13}C-NMR$ and MS data. The DPPH radical scavenging activity$(RC_{50}:\;13.3{\mu}g)$ of apigenin was similar to that of ${\alpha}-tocopherol\;(12.1{\mu}g)$ and L-ascorbic acid$(13.1{\mu}g)$

• Korean Journal of Pharmacognosy
• /
• v.15 no.2
• /
• pp.74-77
• /
• 1984

Cirsium xanthocanthum (Compositae) is a perennial herb. From the roots of this plant which were used for arthritis, neuralgia and carbuncular abscess as folkloric remedies, linarin, mp $255{\sim}257^{\circ}$, was isolated and identified by chemical reactions and spectral data with authentic samples.

• Korean Journal of Pharmacognosy
• /
• v.22 no.3
• /
• pp.166-170
• /
• 1991

Four compounds were isolated from the leaves and stems of Lycopus lucidus and identified as $2{\alpha}-hydroxy$ ursolic acid, tormentic acid, ${\beta}-sitosterol$ glucoside and linarin by spectroscopic methods.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.24 no.6
• /
• pp.906-910
• /
• 1995

Two flavone glycosides have been obtained from the aerial part of Cirsium japonicum var. ussuriense Kitamura(Co,positae) in Korea and identified by means of spectral analysis as linarin and cirsimarin. When 10-2mg/ml of cirsimas was added, lipid peroxide formation in the rat liver decreased by 12% compare to control.

• Korean Journal of Pharmacognosy
• /
• v.31 no.2
• /
• pp.157-162
• /
• 2000

The herb of Melampyrum roseum var. hirsutum Beauv. was extracted with methanol and the extract was fractionated with n-hexane, chloroform, ethylacetate and n-buthanol. Repeated column chromatography of silica gel, Sephadex LH-20 and Lobar-A lichroprep Si 60 led to the isolation of five compounds from n-hexane layer and ethylacetate layer. These compounds were identified as apigenin, linarin, pectolinarin, wogonin and mussaenoside, respectively, by the physicochemical and spectral properties.

• Korean Journal of Pharmacognosy
• /
• v.48 no.1
• /
• pp.70-76
• /
• 2017

Dendranthema spp are distributed in various regions in Korea. This report is the quantitative results of phenolic compounds on the 5 resources collected from all over the country. It was known that it has phenolic compounds, as an anticoagulant activities and immunoadjuvant activity of contribute to the cardiovascular effects. This was used for quantitative analysis of chlorogenic acid(CA), 3,5-Dicaffeoylquinic acid(3,5-DCA), 4,5-Dicaffeoylquinic acid(4,5-DCA) and linarin, with UPLC-PDA and LC-IT-TOF-MS. Extraction efficiency of compounds was compared by using ultrasonic extraction and reflux extraction with different extraction conditions (methods and time). In 50% MeOH extracs (30 mins) of Dendranthema zawadskii (Herb.) Tzvelev (SDZ), Dendranthema zawadskii var. yezoense (Maek.) Y.M.Lee & H.J.Choi (NDZ), Dendranthema zawadskii (Herb.) Tzvelev var. teuisectum (Kitag.) J. H. Pak (GDZ), Dendranthema careanum (H. $L{\acute{e}}v$. & Vaniot) Vorosch. (HDC) and Dendranthema zawadskii var. tenuisectum Kitag (PDZ), chlorogenic acid contents were 1.14‰, 6.95‰, 7.27‰, 1.47‰ and 2.64‰, 3,5-dicaffeoylquinic acid contents were 3.30‰, 5.60‰, 10.81‰, 2.67‰ and 1.50‰, 4,5-dicaffeoylquinic acid contents were 0.74‰, 1.93‰, 3.36‰, 0.61‰ and 0.43‰ and linarin contents were 3.90‰, 10.15‰, 0.15‰, 0.73‰ and 0.21‰, respectively.

• Proceedings of the PSK Conference
• /
• 2000.10a
• /
• pp.179.1-179.1
• /
• 2000

• Proceedings of the PSK Conference
• /
• 2001.04a
• /
• pp.165.3-166
• /
• 2001