Quantitative Analysis about Phenolic Compounds of Dendranthema Species in Korean Native Plants

국내 자생 구절초속 중 페놀화합물 함량분석

  • Kim, Kyeong Mi (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Lee, Seon Yu (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Jo, Ara (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Kang, Byoung Man (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Ham, Seongho (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Cho, Junghee (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Lee, Gukyeo (Division of Traditional Korean Medicine Resource, NIKOM)
  • 김경미 (한약진흥재단 한약자원본부) ;
  • 이선유 (한약진흥재단 한약자원본부) ;
  • 조아라 (한약진흥재단 한약자원본부) ;
  • 강병만 (한약진흥재단 한약자원본부) ;
  • 함성호 (한약진흥재단 한약자원본부) ;
  • 조정희 (한약진흥재단 한약자원본부) ;
  • 이국여 (한약진흥재단 한약자원본부)
  • Received : 2016.09.09
  • Accepted : 2017.03.21
  • Published : 2017.03.31

Abstract

Dendranthema spp are distributed in various regions in Korea. This report is the quantitative results of phenolic compounds on the 5 resources collected from all over the country. It was known that it has phenolic compounds, as an anticoagulant activities and immunoadjuvant activity of contribute to the cardiovascular effects. This was used for quantitative analysis of chlorogenic acid(CA), 3,5-Dicaffeoylquinic acid(3,5-DCA), 4,5-Dicaffeoylquinic acid(4,5-DCA) and linarin, with UPLC-PDA and LC-IT-TOF-MS. Extraction efficiency of compounds was compared by using ultrasonic extraction and reflux extraction with different extraction conditions (methods and time). In 50% MeOH extracs (30 mins) of Dendranthema zawadskii (Herb.) Tzvelev (SDZ), Dendranthema zawadskii var. yezoense (Maek.) Y.M.Lee & H.J.Choi (NDZ), Dendranthema zawadskii (Herb.) Tzvelev var. teuisectum (Kitag.) J. H. Pak (GDZ), Dendranthema careanum (H. $L{\acute{e}}v$. & Vaniot) Vorosch. (HDC) and Dendranthema zawadskii var. tenuisectum Kitag (PDZ), chlorogenic acid contents were 1.14‰, 6.95‰, 7.27‰, 1.47‰ and 2.64‰, 3,5-dicaffeoylquinic acid contents were 3.30‰, 5.60‰, 10.81‰, 2.67‰ and 1.50‰, 4,5-dicaffeoylquinic acid contents were 0.74‰, 1.93‰, 3.36‰, 0.61‰ and 0.43‰ and linarin contents were 3.90‰, 10.15‰, 0.15‰, 0.73‰ and 0.21‰, respectively.

Keywords

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