Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Quantitative Analysis about Phenolic Compounds of Dendranthema Species in Korean Native Plants

국내 자생 구절초속 중 페놀화합물 함량분석

  • Kim, Kyeong Mi (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Lee, Seon Yu (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Jo, Ara (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Kang, Byoung Man (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Ham, Seongho (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Cho, Junghee (Division of Traditional Korean Medicine Resource, NIKOM) ;
  • Lee, Gukyeo (Division of Traditional Korean Medicine Resource, NIKOM)
  • 김경미 (한약진흥재단 한약자원본부) ;
  • 이선유 (한약진흥재단 한약자원본부) ;
  • 조아라 (한약진흥재단 한약자원본부) ;
  • 강병만 (한약진흥재단 한약자원본부) ;
  • 함성호 (한약진흥재단 한약자원본부) ;
  • 조정희 (한약진흥재단 한약자원본부) ;
  • 이국여 (한약진흥재단 한약자원본부)
  • Received : 2016.09.09
  • Accepted : 2017.03.21
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Dendranthema spp are distributed in various regions in Korea. This report is the quantitative results of phenolic compounds on the 5 resources collected from all over the country. It was known that it has phenolic compounds, as an anticoagulant activities and immunoadjuvant activity of contribute to the cardiovascular effects. This was used for quantitative analysis of chlorogenic acid(CA), 3,5-Dicaffeoylquinic acid(3,5-DCA), 4,5-Dicaffeoylquinic acid(4,5-DCA) and linarin, with UPLC-PDA and LC-IT-TOF-MS. Extraction efficiency of compounds was compared by using ultrasonic extraction and reflux extraction with different extraction conditions (methods and time). In 50% MeOH extracs (30 mins) of Dendranthema zawadskii (Herb.) Tzvelev (SDZ), Dendranthema zawadskii var. yezoense (Maek.) Y.M.Lee & H.J.Choi (NDZ), Dendranthema zawadskii (Herb.) Tzvelev var. teuisectum (Kitag.) J. H. Pak (GDZ), Dendranthema careanum (H. $L{\acute{e}}v$. & Vaniot) Vorosch. (HDC) and Dendranthema zawadskii var. tenuisectum Kitag (PDZ), chlorogenic acid contents were 1.14‰, 6.95‰, 7.27‰, 1.47‰ and 2.64‰, 3,5-dicaffeoylquinic acid contents were 3.30‰, 5.60‰, 10.81‰, 2.67‰ and 1.50‰, 4,5-dicaffeoylquinic acid contents were 0.74‰, 1.93‰, 3.36‰, 0.61‰ and 0.43‰ and linarin contents were 3.90‰, 10.15‰, 0.15‰, 0.73‰ and 0.21‰, respectively.

Keywords

References

  1. 이우철 (1996) 원색한국기준식물도감, 아카데미서적, 서울.
  2. Sato, Y., Itagaki, S., Kurokawa, T., Ogura, J., Kobayashi, M., Hirano, T., Sugawara, M. and Iseki, K. (2010) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int. J. Pharm. 403: 136-138.
  3. Karunanidhi, A., Thomas, R., van Belkum, A., and Neela, V. (2013) In Vitro Antibacterial and Antibiofilm Activities of Chlorogenic Acid against Clinical Isolates of Stenotrophomonas maltophilia including the Trimethoprim/Sulfamethoxazole Resistant Strain. Biomed Res Int. 392058: 1-7.
  4. Belkaid, A., Currie, J. C., Desgagnes, J. and Annabi, B. (2006) The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-60phosphate translocase in brain tumor progression. Cancer cell Int. 6: 7-7. https://doi.org/10.1186/1475-2867-6-7
  5. Wu, Q. Z., Zhao, D. X., Xiang, J., Zhang, M., Zhang, C. F. and Xu, X. H. (2016) Antitussive, expectorant, and antiinflammatory activities of four caffeoylquinic acids isolated from Tussilago farfara. Pharm. Biol. 54: 1117-1124. https://doi.org/10.3109/13880209.2015.1075048
  6. Woo, J. H., Shin, S. L., Chang, Y. D. and Lee, C. H. (2010) Antioxidant effect according to extraction method in extracts of Dendranthema zawadskii var. yezoense and Cosmos bipinnatus. Kor. J. Hort. Sci. Technol. 28: 462-468.
  7. Hyum, M. R., Lee, Y. S. and Park, Y. H. (2011) Antioxidative activity and flavonoid content of chrysanthemum zawadskii flowers. Kor. J. Hort. Sci. Technol. 29: 68-73.
  8. Hwang, J. L., Kim, J. M., Kim, M. S., Kim, H. R., Park, Y. J., Yon, Y. O., Kwon, K. B. and Lee, Y. R. (2013) Chrysanthemum zawadskii var. latilobum extracts inhibits of TPAinduced invasion by reducing MMP-9 expression via the suppression of NF-${\kappa}B$ activation in MCF-7 human breast carcinoma cells. Korean. J. Oriental Physiology & Pathology 27: 782-788.
  9. Jang, D. S., Park, K. H., Choi, S. U., Nam, S. H. and Yang, M. S. (1997) Antibacterial substances of the flower of Chrysanthemum zawadskii Herbich var. latilobum Kitamura. Korean Agricultural Chemistry and Biotechnology 40: 85-88.
  10. 식품의약품안전처 (2015) 대한민국약전외한약(생약)규격집(고시 제 2015-96호), 45-46, 서울.
  11. 이창복 (2003) 원색 대한식물도감(하), 377-378, 향문사, 서울.
  12. Park, Y. C., Kim, S. R. and Han, T. W. (2004) Plant regeneration from leaf segment culture of Chrysanthemum zawadskii ssp. coreanum. Korean J. Plant Res. 17: 43-47.
  13. Chung, H. J. and Jeon, I. S. (2011) Antioxidative activities of methanol extracts from different parts of Chrysanthemum zawadskii. Korean J. Food Preserv. 18: 739-745. https://doi.org/10.11002/kjfp.2011.18.5.739
  14. 대교 (2008) 눈높이대백과, http://newdle.noonnoppi.com/xmlView.Aspx?xmldid=17749, 서울.
  15. 국가표준식물목록. http://www.nature.go.kr/kpni/index.do.
  16. Zhao, H. B., Chen, F. D., Chen, S. M., Wu, G. S. and Guo, W. M. (2010) Molecular phylogeny of Chrysanthemum, Ajania and its allies (Anthemideae, Asteraceae) as inferred from nuclear ribosomal ITS and chloroplast trnL-F IGS sequences. Plant Syst. Evol. 284: 153-169. https://doi.org/10.1007/s00606-009-0242-0
  17. Kim, T. J., Lee, T. R. and Park, H. K. (1991) Simultaneous determination of chlorogenic acid and linarin in chrysanthemum sibiricum fisher by reversed-phase high performance liquid chromatography. Kor. Chemical Society 35: 720-724.
  18. 식품의약품안전처 (2015) 대한민국약전 제11개정 약전, 1654-1656, 서울.
  19. Lee, K. Y. (1993) Antioxidant effects of phenolic compounds isolated from deffated perilla seed flour. Korean J. Food. SCI. Technol. 25: 9-14.
  20. Kim, Y. Y., Park, Y. K., Oh, S. R. and Moon, K. D. (2001) Biological activities of linarin from Chrysanthemum zawadskii var. latilobum. Yakhak Heoji 45: 604-610.